Improving food safety through the development and implementation of active and biodegradable food packaging systems - ACTIBIOSAFE 1SEE/30.06.2014 Projects financed under health & food safety and renewable energy domains Dr. Cornelia Vasile “P.Poni” Institute of Macromolecular Chemistry International Conference “Achievements and future steps” Bucharest, December 10, 2015
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Improving food safety through the development and
implementation of active and biodegradable food
packaging systems - ACTIBIOSAFE 1SEE/30.06.2014
Projects financed under health & food safety and
renewable energy domains
Dr. Cornelia Vasile
“P.Poni” Institute of Macromolecular Chemistry
International Conference “Achievements and future steps”
Bucharest, December 10, 2015
Project summary
Programme RO14 - “Research within Priority Sectors”
Conference “Achievements and future steps”
Bucharest, December 10, 2015
Promoter “Petru Poni” Institute of Macromolecular Chemistry - Iasi (PPIMC);
Principal Investigator: Prof. Dr. Cornelia VASILE
Project Partners: 1: NOFIMA AS - Team Leader: Dr. Morten SIVERTSVIK ;
2: SC Research Institute of Organic and Auxiliary Products SA (ICPAO);
Team Leader: Dr. Maria RAPA;
3: SC RODAX SRL (RODAX); Team Leader: Dr. Laurentiu MOLDOVAN;
4: University of Agronomic Sciences and Veterinary Medicine Bucharest (USAMVB);
Team Leader: Ass. Prof. Dr. Amalia MITELUT
Budget: 900 000 Eur. Duration: 2014-2017
Objective(s): Scientific: Effective knowledge sharing among the research and scientific communities: between Romania and Norway;
Technological: Realization of packaging prototypes: biodegradable film and trays, encapsulated forms for bioactive packaging
Improve the level of consumers awareness and health: Obtaining of nanostructured encapsulated systems based on
natural oils (essential and cold-pressed oils) and chitosan/ chitosan derivatives to improve the thermal stability and to
manufacture the biodegradable materials with antioxidant/antimicrobial/antifungal or/and biological activities;
Reducing food contamination risk and prolong shelf life of food products;
Environment protection: Decrease of food waste
Training: Courses elaboration, Supporting PhD theses and MSc students dissertations
Target groups/end-users of projects results: food packaging sectors; food industry, delivery/retail systems for food products.
Research groups:PPIMC – IASI
• C. Vasile• L. Profire• R. Darie-Niţă• B. Munteanu• M. Brebu• E. Stoleru• R.P. Dumitriu• A. Sdrobis• E. Părpăriță• D. Pamfil• External co-workers• O. Dragostin• G. M. Pricope, • E. G. Ioanid• T. Zaharescu• L. Oprică• F. Doroftei
J. T. Rosnes, M. Sivertsvik, T. Løvdal, B. T. Rotabakk
ICPAO – Medias
M. RâpăE. GrosuL. Stanulet A.TrifoiT. PapO. Blajan
RODAX - Bucharest
G. PanteaL. MoldovanM. BeceaL. Magdan
USAMV - Bucharest
A. C. MiteluțM. E. PopaM. C. DrăghiciS. Danaila-GuideaM. Geicu-CristeaE. E. Tănase,P. Popescu
Conference “Achievements and future steps”
Bucharest, December 10, 2015
Project Objectives
ACTIBIOSAFE main purpose is to prolong the shelf life of real food, (chicken and salmon fillets etc. at least 20 % increase), keeping the food quality and freshness during the time required for its commercialization and consumption, to be safe and to have no risk of affecting health.
• Effective knowledge sharing the research results among scientific communities: between Romania and Norway;
• Obtaining of nanostructured encapsulated systems based on natural oils (essential and cold-pressed oils) with chitosan and chitosan derivatives in order to improve the thermal stability and to manufacture the biodegradable materials with antioxidant/ antimicrobial/ antifungal or/and biological activities;
• Minimizing food packaging waste (millions of tons every year) and to preserve natural resources through replacing traditional plastic packaging materials with new biodegradable antimicrobial packaging biocomposites;
• Reducing food contamination risk and prolong shelf life of food products;
• Developing and implementing courses and supporting for individual PhD and MSc student projects;
• High level scientific publications
Conference “Achievements and future steps”
Bucharest, December 10, 2015
Project Objectives
• Integration of technologies:
– extrusion, thermoforming,
– activated coating by gamma irradiation/ plasma exposure,
– Electrospinning/electrospraying coating with bioactive agents
• Industrial validation of prototypes by in vivo and on food packaging equipments tests.
Conference “Achievements and future steps”
Bucharest, December 10, 2015
• WP1: Screening and characterization of raw materials
• WP2: Processes development at laboratory scale and characterization
• WP 3: Development of active food packaging
• WP 4: Quality and Safety Validation of active food packaging systems
• WP5: Dissemination, Exploitation and IPR Management
• WP6: Consortium Management
WORK PACKAGES:
Conference “Achievements and future steps”
Bucharest, December 10, 2015
Inter-multi-and trans-disciplinary key activities
• a) development of new formulations based on biodegradable polymers and chitosan/chitosan derivatives by melt-mixing processing, to meet requirements of mechanical and thermal properties and stability, cost effective and testing them from biological and toxicological point of view
• b) elaboration of methods to obtain encapsulated nanostructures. Active oils of natural substances and natural compounds (cinnamon, thyme, basil, oregano, eugenol as natural extracts, etc.) will be encapsulated into chitosan. Nanostructured materials will be obtained by optimization of electrospinning/spraying conditions.
• c) development of kinetic models for volatile compounds release to foodstuff, antioxidant and antimicrobial/antifungal efficiency, and stability.
• d) to achieve of new food packaging with antimicrobial /antioxidant /bioactive features by integration of technologies (extrusion, thermoforming, electrospinning, electrospraying, active packaging). To prove the viability of the new food packaging material, validation of the packaging prototypes will be carried out in vivo tests on real food products (shelf-life studies, physical-chemical, microbiological and sensorial evaluation) and by testing on food packaging equipments.
• e) create a consortium with producers and consumers from delivery/retail system to
implement the new materials on market.
Conference “Achievements and future steps”
Bucharest, December 10, 2015
Project outcomes (planned vs. achieved)
[…your text in max. 2 bullets:
outcomes to date …]
expected outcomes
Programme RO14 - “Research within Priority Sectors”
Conference “Achievements and future steps”
Bucharest, December 10, 2015
…
Expected outcomes Outcomes to date – 3 gold medals at EUROINVENT 2015
• Selection and testing of bioactive compounds
• Chemical modification of chitosan
• Obtaining of biodegra dable,
bioactive packaging materials –composites, nanocomposites, stratified composites and encapsulated formulations with antimicrobial /antifungal /antioxidant/biological functions
• Training of young researchers and students – PhD students and Master students
• Chitosan and eight vegetable oils were tested for their composition, antimicrobial/antifungal and antioxidant activity – 1 paper accepted for publication
• Sulfadiazine- and other chitosan derivatives are obtained by original procedures – 1 ISI paper published
• Three ways are developing at laboratory/micropilot scale:
- melt processing , selection of plasticizers for PLA/PHB/PHBV – 3 ISI co-
authored papers in press- coating by a two step procedure: gamma irradiation or cold plasma pre-
treatment followed by immersion or electrospinning/coupling reaction – 1 co-authored paper sent to ISI journal – gold medal at EUROINVENT 2015
- freezing-thawing method – 1 ISI paper in press and 1 book chapter published
• 1 PhD Thesis and 2 MSc dissertations defended; international ERASMUS courses on Application of Radiation technology to food packaging materials and 3 chapters in a book ready for evaluation before sending to press
Characterization of bioactive agents
• Determination of total content of phenols and flavonoids of essential and cold press- bioactive oils
• Determination of average composition of essential oils by GC-MS
• Determination of antioxidant/radical scavenger activity of bioactive compounds by 2,2-difenil-1-picrilhidrazil (DPPH)) and acid 2,2`-azino-bis(3-etilbenzotiazolin-6-sulfonic) (ABTS), methods
• Determination of antimicrobial activity by standardized (Food safety accreditated laboratory) and new developed methods (NOFIMA)
Conference “Achievements and future steps”
Bucharest, December 10, 2015
Conference “Achievements and future steps”
Bucharest, December 10, 2015
• Essential oils:
• - Thyme F (Thymus Vulgaris) – Fares company; - Cloves F
(Caryophylli aetheroleum, dried flower buds of Syzygium
aromaticum) ; - Rosemary F (Rosmarini Aetheroleum); Ti
Tree F (Melaleuca alternifolia aetheroleum) – Fares
Tasks 2.2 Preparation and optimization of PHA (PLA)/ CS formulations
Tasks 2.3. Caracterization of the developed composites
Conference “Achievements and future steps”
Bucharest, December 10, 2015
I) Melt processing: Optima formulations have been established:
Plasticized PLA properties
(a) (b)
(c)
PLA plasticization with about 15 % non-toxic selected plasticizers could yield flexible and transparent films (PLA/USE) with appropriate optical and barrier properties (PLA/PEG) for applications in the food-packaging.
Conference “Achievements and future steps”
Bucharest, December 10, 2015
Analyses of antimicrobial effects from treated packaging films
• 3 gold medals and 1 Excellence diploma to EUROINVENT’2015
• 8 oral communications and 12 posters to international conferences
Conference “Achievements and future steps”
Bucharest, December 10, 2015
Task 2.9. Dissemination of the resultsby participation at conferences andpublication of 2 ISI papers
1. Sulfadiazine—Chitosan Conjugates and Their Polyelectrolyte Complexes with Hyaluronate Destined to the Management of Burn Wounds, R. P. Dumitriu, L. Profire, L. E. Nita, O. M. Dragostin, N. Ghetu, D. Pieptu and C. Vasile, Materials 2015, 8, doi:10.3390/ma70x000x
2. Evaluation of some eco-friendly plasticizers for PLA films processing, R.N. Darie-Nita, C. Vasile, R. Lipsa, M. Rapa, J. Appl. Polym. Sci. in press
3. Poly(vinyl alcohol)/chitosan/montmorillonite nanocomposites for food packaging applications: Influence of montmorillonite content. E. Butnaru, C. N. Cheaburu, O. Yilmaz, G. M. Pricope and C. Vasile, High Performance Polymers, DOI: 10.1177/0954008315617231.
Published Papers ISI Journals
Conference “Achievements and future steps”
Bucharest, December 10, 2015
Chapters in books
Eco-Friendly Chitosan-Based Nanocomposites:
Chemistry and Applications,
C. N. Cheaburu-Yilmaz, O. Yilmaz, and C, Vasile, Chapter in Eco-friendly
Polymer Nanocomposites. Chemistry and Applications, Eds, V. Kumar Thakur, M. K. Thakur, 341-387 , 2015, Springer, ISSN 1869-8433 ISSN 1869-8441 (electronic), Advanced Structured Materials, ISBN 978-81-322-2472-3 ISBN 978-81-322-2473-0 (eBook)
DOI 10.1007/978-81-322-2473-0, Springer New Delhi, Heidelberg, New York, Dordrecht London,http://www.springer.com,series/8611
• Effect of plasticizers on melt processability and properties of PHB, M. Râpă, R.N. Darie-Niță, E. Grosu, E.E.Tănase, A.R. Trifoi, T. Pap, C. Vasile J.Opto. Adv. Mater.
• Sustainable Alternative for Food Packaging :
Chitosan Biopolymer – a review, A. C.
Miteluţ, E. E. Tănase, V. I. Popa, M. E. Popa,
AgroLife Scientific Journal, Vol 4, No 2, 2015
Conference “Achievements and future steps”
Bucharest, December 10, 2015
Participation to international and national meetings
• 9th International Conference on Materials Science and Engineering –BRAMAT 2015, Brasov, Romania
• Oral presentation:
• II.O.10. Influence of plasticizers over some physico-chemical properties of PLA• R.N. DARIE-NIȚĂ, C. VASILE, PPIMC, Iasi, ROMANIA; M. RÂPĂ, ICPAO S.A., Medias, ROMANIA• Posters:• II.P.64. Assessment of the antifungal activity of chitosan films for new packaging materials
design• A.C. MITELUT, E.E. TANASE, A.L. MIHAI, C.P. CORNEA, M.E. POPA, M.C. DRAGHICI, University
of Agronomic Sciences and Veterinary Medicine Bucharest, ROMANIA; E. STOLERU, B.S. MUNTEANU PPICM, ROMANIA
• II.P.77. Effect of plasticizers on melt processability and properties of PHB • M. RÂPĂ, E. GROSU, A.R. TRIFOI, T. PAP,ICPAO S.A., Medias, ROMANIA; R.N. DARIE-NIȚĂ, C.
VASILE, PPIMC Iasi, ROMANIA; E.E. TĂNASE, USAMV- Bucharest, ROMANIA• II.P.92. New PLA based composites and their behaviour for food packaging applications• E.E. TĂNASE, M.E. POPA, O. POPA, USAMV, Bucharest, ROMANIA; M. RÂPĂ, S.C. I.C.P.E.
BISTRITA S.A., Bistrita, ROMANIA
Conference “Achievements and future steps”
Bucharest, December 10, 2015
COST Action MP1206 Workshop: Applications of Electrospinning in Composites, Nanofabrication, Food packaging, Pharma and Controlled Release, March, 25-27, 2015, Novi Sad,Serbia.
Oral presentations
1. Sulfadiazine release from electrospun meshes, B.S. Munteanu, R.P.
Dumitriu,E. Stoleru, L. Profire, C. Vasile – oral
2. Comparative study between electrospinning and immersion methods for
chitosan deposition onto poly(lactic acid) E. Stoleru, B.S. Munteanu, G.M.
Pricope, C. Vasile - oral.
3. Antibacterial membranes based on polyurethane/biological polymers/silver
nanoparticles, B.S. Munteanu, E. Stoleru, R.P. Dumitriu, G.M. Pricope, D.
Macocinschi, D. Filip, C. Vasile - oral.
Conference “Achievements and future steps”
Bucharest, December 10, 2015
EUROINVENT May, 14-16, 2015 Iasi
NEEFOOD – Brasov, May 2015
ORAL1, ENG. 6 Development of innovative biodegradable packaging system to improve
shelf life, quality and safety of fresh products, L. Moldovan, G. Pantea – RODAX Bucharest
2. ENG. 8 FOOD PACKAGING MATERIAL BASED ON CHITOSAN AND POLY(LACTIC ACID) E. Stoleru, B. S. Munteanu, R. N. Darie-Niță, G. M. Pricope, E. G. Ioanid, C. Vasile – PPIMC IasiA.C. Miteluț, M. E. Popa, E. E. Tănase, A. L. Mihai, M. C. Drăghici –USAMVBucharest
3. ENG. 9 CHITOSAN/NATURAL OILS AS COMPONENTS IN INNOVATIVE FORMULATIONS FOR FOOD PACKAGING, C. Vasile, B.S. Munteanu, M. Brebu, E. Stoleru, R. N. Darie-Nita – PPIMC - IasiA. C. Miteluț, M. E. Popa, E. E. Tănase, A. L. Mihai, M. C. Drăghici USAMVBJ. T. Rosnes, M. Sivertsvik, T. Løvdal, B. T. Rotabakk –NOFIMA
4. ENG. 7 POLY(VINYL ALCOHOL)/CHITOSAN NANOCOMPOSITES FOR FOOD PACKAGING APPLICATIONSE. Parparita, C- N.Yilmaz, O. Yilmaz, G-M. Pricope, C. Vasile – PPIMC
Conference “Achievements and future steps”
Bucharest, December 10, 2015
Posters• POS. 4. Assessment of the Antifungal Activity of Essential Oils for New
Food Packaging Materials Design, A. C. Mitelut, A. L. Mihai, E. E. Tănase, M. E. Popa, M. Drăghici, C. P. Cornea, M. E. Popa, M. Draghici, M. A. Brebu, C. Vasile, E. Stoleru, A. Irimia. Neefood proceedings.
• POS. 58. Antimicrobial Resistance of Staphylococcus aureus and E. coli to Essential Oils, J. T. Rosnes, L. Shinde, C. Vasile, M. A. Brebu. Neefood proceedings.
• POS. 3. Chitosan as a Biopolymer for Food Packaging Applications - A Review, A. C.Mitelut, E. E. Tănase, M. E. Popa, V. I. Popa. Neefood proceedings.
• POS. 15. Food Packaging Materials: Current Trends and Future Opportunities. E. E. Tanase, M. E. Popa, O. Popa, M. Rapa, Neefood proceedings.
The 14th International Symposium Prospects for the 3rd Millennium Agriculture, Improving food safety through the development and implementation of active and biodegradable food packaging systems. A.C. Miteluţ, M. E. Popa, E. E. Tănase, M. C. Drăghici, 24-26 September 2015, Cluj Napoca, Romania.
NEEFOOD – Brasov May 2015 continued
Conference “Achievements and future steps”
Bucharest, December 10, 2015
Papers submitted for publication
• Influence of Plasticizers Over Some Physico-chemical Properties of PLA, M. Râpă, R. N. Darie-Niță, C. Vasile, JOAM
• Novel procedure to enhance PLA surface properties by chitosan irreversible immobilization, E.Stoleru, R. P.Dumitriu, B. S. Munteanu, T. Zaharescu, E. E. Tănase, A. Mitelut, G.-L. Ailiesei, C. Vasile, Appl. Sur. Sci.
Conference “Achievements and future steps”
Bucharest, December 10, 2015
Reflections on partnership: role and contributions to the project
Programme RO14 - “Research within Priority Sectors”
Conference “Achievements and future steps”
Bucharest, December 10, 2015
PP and P2: elaboration of formulations for bioactive packaging and their general characterization, elaboration of prototypes
P1: antimicrobial and migration tests
P4: antifungal test, ecotoxicity and degradability
Strong points: Strong and ethical collaboration based on: deep consideration, loyalty, partners expertise, confidence in quality of results. It started since 2008 in the framework of one FP7 project; research teams is composed by experts in their fields of interest which are interrelated. Weak points: The formulations properties should be modulated to correlate their good bioactive
activities with mechanical, permeability and migration properties What could be improved? By detailed study of relationship: composition of formulations –
properties.
P3 and P1: Upscale the research results and testing on
packaging manufacture equipments and specific characterization.
Market analysis and consumers demands evaluation
Future steps
• Future steps:- Detailed characterization of bioactive biodegradable materials obtained - Development of the nanosized encapsulated structures;- Modernization and improved functionality of electrospinning
device- Continue dissemination of results by publications, website
up-date, leaflets, newsletters, brochures, etc. sustainability of consortium: we intend to continue our collaboration
beyond this project by application to H2020, joint applications, co-authored publications, etc.
There are already proposals in MNT-ERA projects in second stage. PP is partner in two projects: IAEA and Erasmus with similar topics or related
with this, where project results are disseminated; NOFIMA also have 24
proposals (4 European ,10 regional, 8 national and 2 in other programmes)
including some aspects of this topic and training.
Programme RO14 - “Research within Priority Sectors”
Conference “Achievements and future steps”
Bucharest, December 10, 2015
Thanks for inviting us at this prestigious conference,
for financial support from European Economic Area
(EEA) and Norwegian Grants, Romanian National
Authority for Scientific Research and Innovation
(ANCSI) and Romanian Ministry of European Funds
Thanks for your kind attention!
Programme RO14 - “Research within Priority Sectors”