Improvement of insect ecdysome on the hatching rate of Artemia eggs Jia Qinxian 1 Chen Lijing 2 Zhen Mianping 1 1 Open Laboratory of Saline Lake Resources and Environment, The Chinese Academy of Geological Sciences, Beijing, China 2 Shanghai Fisheries University, Shanghai, China
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Improvement of insect ecdysome on the hatching rate of Artemia eggs Jia Qinxian 1 Chen Lijing 2 Zhen Mianping 1 1 Open Laboratory of Saline Lake Resources.
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Improvement of insect ecdysome on the hatching rate of Artemia eggs
Jia Qinxian1 Chen Lijing2 Zhen Mianping1
1 Open Laboratory of Saline Lake Resources and Environment, The Chinese Academy of Geological Sciences, Beijing, China
2 Shanghai Fisheries University, Shanghai, China
Content
1. Introduction 2.Treatment and Establish normal
criterion method 3. Results 4. Conclusion
1.Introduction
Concept : Diapause and Dormancy
DDIIAAPPAAUUSSEE DDOORRMMAANNCCYY
Conditionunsuitability
No developing No developing
Condition suitability No developing Developing
Regulation Physiological Circumstance
Control mechanism Nerve system -hormone-body fluid-target cells
Conditionreflection
Diapause----- 生长的停滞期,间歇期Dormancy---- 睡眠,冬眠,隐匿
1. Introduction:Understanding of Artemia eggs structure
Outer membrane
Outer lay shuck
Inner lay shuckEmbryonEmbryonic membrane
Inner membrane
1. Introduction:Understanding of Artemia eggs structure
Embryon
Embryonic membrane
1. Introduction:Study progress of Artemia eggs diapause (1)
Structure
Outer membrane
Diapause Activation
Morris and Afzelius (1967): Outer membrane brokenAnderson et al. (1970) : shuck thinned
1.Introduction:Study progress of Artemia eggs diapause (2)
Structure
Diapause
Lavens and Sorgeloos (1987)
Diapause
New productive eggsDecapsulation
but no activation
1.Introduction:Study progress of Artemia eggs diapause (3)
pHi
pHi
pHi>7.9 diapause eggs
Diapause Activation
pHi
Some scholars find that the eggs was diapause at pHi over7.9 and guess the diapause eggs will activation at pHi reduced to acidity
1. Introduction:Study progress of Artemia eggs diapause (4)
Biotic active matter
Biotic activematter
Dutrieu (1960)Nelis et al. (1984 a,b; 1987)Konrad Dabrowski (19991)
Biotic active mater have important regulation for diapause eggs activition
1. Introduction:Study progress of Artemia eggs diapause (5)
problem
The Artemia eggs diapause mechanism not be complete comprehended
The methods of activation of diapause eggs was notalways efficiency for all strains
Up to now, there are a great lot of low hatching quality eggs produced in Asia ,Europe and other places. Which should be improvement.
(Collected and experimental time of Artemia eggs: 2000~2001)
Efficiency compare of different treatment method
0
10
20
30
40
50
60
70
80
90
100
Not treatmentDecapsul ati onNaOHHClCH3OHFreezeHormone
Not treatment 20 45 12
Decapsul ati on 71 74 35
NaOH 56 50 46
HCl 38 42 35
CH3OH 59 44 25
Freeze 74 80 64
Hormone 85 90 72
SHY QGH KSK
0
20
40
60
80
100
SHY QGH KSK RUS
Not treatment
Decapsulation
NaOH
HCl
H2O2
CH3OH
Freeze
Hormone
Hat
chin
g ra
te
4.Conclusion The superexcellent concentration and
fitness treatment time of each strain is difference.
The raise of the hatching quality is very obvious at its fit concentration and time.
The shortage concentration of hormone will no obvious effect on most strains
4.Conclusion More over concentration of hormone will
obvious decrease the hatching rate. When the concentration over 35 ppb the hatching rate was maybe littler than that of no treatment
At the ecdysome concentration range 10 to 20 ppb and the treatment time range 30 to 60 min, the hatching rate of most strains was greatly improved.
4.Conclusion
At superexcellent concentration and fitness treatment time, the hormone treatment was higher than the treatment by chemical reagent and physical method (-25 freeze 4 ℃to 20 weeks).
The highest hatching of Haiyang strain was over 57.1% than no treatment, over 10.5% than treatment by H2O2 and freeze.