Heriot-Watt University Research Gateway Improved bovine embryo production in an oviduct-on-a-chip system: prevention of poly-spermic fertilization and parthenogenic activation Citation for published version: Ferraz, MAMM, Henning, HHW, Costa, PF, Malda, J, Melchels, F, Wubbolts, R, Stout, TAE, Vos, PLAM & Gadella, BM 2017, 'Improved bovine embryo production in an oviduct-on-a-chip system: prevention of poly- spermic fertilization and parthenogenic activation', Lab on a Chip, vol. 17, no. 5, pp. 905-916. https://doi.org/10.1039/C6LC01566B Digital Object Identifier (DOI): 10.1039/C6LC01566B Link: Link to publication record in Heriot-Watt Research Portal Document Version: Peer reviewed version Published In: Lab on a Chip General rights Copyright for the publications made accessible via Heriot-Watt Research Portal is retained by the author(s) and / or other copyright owners and it is a condition of accessing these publications that users recognise and abide by the legal requirements associated with these rights. Take down policy Heriot-Watt University has made every reasonable effort to ensure that the content in Heriot-Watt Research Portal complies with UK legislation. If you believe that the public display of this file breaches copyright please contact [email protected] providing details, and we will remove access to the work immediately and investigate your claim. Download date: 28. Jul. 2022
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Heriot-Watt University Research Gateway
Improved bovine embryo production in an oviduct-on-a-chipsystem: prevention of poly-spermic fertilization andparthenogenic activation
Citation for published version:Ferraz, MAMM, Henning, HHW, Costa, PF, Malda, J, Melchels, F, Wubbolts, R, Stout, TAE, Vos, PLAM &Gadella, BM 2017, 'Improved bovine embryo production in an oviduct-on-a-chip system: prevention of poly-spermic fertilization and parthenogenic activation', Lab on a Chip, vol. 17, no. 5, pp. 905-916.https://doi.org/10.1039/C6LC01566B
Digital Object Identifier (DOI):10.1039/C6LC01566B
Link:Link to publication record in Heriot-Watt Research Portal
Document Version:Peer reviewed version
Published In:Lab on a Chip
General rightsCopyright for the publications made accessible via Heriot-Watt Research Portal is retained by the author(s) and /or other copyright owners and it is a condition of accessing these publications that users recognise and abide bythe legal requirements associated with these rights.
Take down policyHeriot-Watt University has made every reasonable effort to ensure that the content in Heriot-Watt ResearchPortal complies with UK legislation. If you believe that the public display of this file breaches copyright pleasecontact [email protected] providing details, and we will remove access to the work immediately andinvestigate your claim.
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ISSN 1473-0197
Lab on a ChipMiniaturisation for chemistry, physics, biology, materials science and bioengineering
PAPERYong Zhang, Chia-Hung Chen et al.Real-time modulated nanoparticle separation with an ultra-large dynamic range
Volume 16 Number 1 7 January 2016 Pages 1–218
Lab on a ChipMiniaturisation for chemistry, physics, biology, materials science and bioengineering
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H. H.W. Henning, P. F. D. Costa, J. Malda, F. W.P. Melchels, R. Wubbolts, T. A.E. Stout, P. L.A.M. Vos and B.
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Figure 1. Perspective visualization of the open device’s 3D printable model from 602 above (A) and below (B). Schematic top (C), bottom (D), right (E) and front (F) view 603 of the open device. Schematic right (G) and front (H) cross section of the closed 604 device while being separately perfused with two (pink and blue) different types of 605 media/cells. Dimensions are represented in millimeters. 606
607 Figure 2. Confocal immune fluorescent images of bovine oviduct epithelial cells 608 (BOECs) in 3D culture at an air-liquid interface for 28 days (A, B and C) and from 609 paraffin sections of oviductal isthmus and ampulla (D and E, respectively). 610 Acetylated α-tubulin was used to stain secondary cilia (green), phalloidin to label 611 actin filaments (red in A, B and C) and Hoechst 33342 to stain nuclei (blue). A, B and 612 C: Note the presence of ciliated cells (green, white arrows), actin rich secretory 613 protrusions (red, yellow arrows) and primary cilia (yellow arrow heads). In B, note 614 the cuboid to columnar pseudostratified epithelium. D and E: Note columnar 615 pseudostratified morphology of oviduct paraffin sections, similar to the one 616 encountered in the 3D cultured BOEC. In paraffin embedded sections the phalloidin 617 staining was not observed. The Z-stacks from top to bottom of the cells cultured on 618 the 3D system can also be observed in the supplementary movie 2. Bars = 25 µm. 619 620 Figure 3. Average percentage of ciliated BOECs in 3D culture during weeks 3, 4, 5 621 and 6 of air-liquid interface culture (n=4 animals). No difference was observed in 622 the percentages of ciliated cells across the period studied (p>0.05). 623 624 Figure 4. Mean percentage of COCs placed in maturation medium that were 625 penetrated by sperm. In vitro fertilization was performed in four replicates using 626 different systems: 3D culture (n=200 COCs), 2D culture (n=200 COCs) and in the 627 absence of oviductal epithelial cells (with or without activation factors; n=300 COCs 628 for each group). Total penetrated: different letters indicate values that differ 629 statistically (p<0.05); Polyspermy: different numbers indicate values that differ 630 statistically (p<0.05); Parthenogenesis: no differences were observed (p>0.05). 631 Activation factors: heparin, penicillamine and hypothaurine. 632 633 Figure 5. Monospermic oocyte penetration; Hoechst 33342 used to stain DNA (blue) 634 and MTG used to label sperm mid pieces (green). Note the presence of maternal and 635 paternal pronuclei (PN), the mid piece (white arrow) of the spermatozoa that 636 penetrated the zona pellucida (ZP) and fertilized the oocyte, and spermatozoa 637 attached to the zona pellucida (blue arrows). Bar = 50 µm. 638 639 Figure 6. Confocal Z-stacks of a polyspermic penetrated oocyte, stained with Hoechst 640 33342 for DNA (blue) and MTG for sperm mid piece (green). Note the presence of 641 multiple pronuclei (PN), the mid piece (white arrows) of two sperm cells that 642 penetrated the zona pellucida (ZP) and fertilized the oocyte; a sperm cell that 643