Implementation of Bovine Brucellosis Eradication Programme in Portugal 2014 PAFF Standing Committee Brussels, 7-8 July 2015
Implementation of
Bovine Brucellosis Eradication Programme
in Portugal 2014
PAFF Standing Committee Brussels, 7-8 July 2015
Regions Number of herds
Number of animals
Norte 17 333 256 472
Centro 6 350 118 247
LVT 1 080 85 409
Alentejo 4 196 533 978
Açores 4 619 131 414
TOTAL 33 578 1 125 520
Approved by Commission Decision 2013/722/UE
Algarve is Officially Free since April 2012 (Commission Decision 2012/204/EU)
Norte
Centro
LVT
Alentejo
Algarve
Bovine Brucellosis eradication programme Characterization 2014 – Mainland & Azores
Officially brucellosis free and Free herds - annual herd screening test Rose Bengal Test (RBT) to all animals over 12 months
If RBT positive results are found samples are submitted to
Complement Fixation Test (CFT) Control of animal movement control and pre-movement testing
Bovine Brucellosis eradication programme Strategy 2014
Bovine Brucellosis eradication programme Strategy 2014
In positive suspended herds (at least 1 animal RB positive and CFT >= 20 IU/mL)
• Farmer is notified by DSAVR - herd is under sanitary sequestration
• Animal movement is prohibited unless for immediate slaughter
• Control of milk according with Reg. 853/2004
• Positive animals are marked, collected and slaughter under official control, in the 30 days following the date of notification
• Samples are collected from slaughtered animals of B3/B4 suspended herds and submitted to bacteriology
• Remaining animals are submitted to a serological control within 30 days after the withdrawal to slaughter of positive animals
• Suspended status is lifted with 2 consecutive negative tests to all animals over 12 months of age, with 30 and 60 days interval
In infected herds:
Epidemiological evaluation and identification of contact herds and possible sources of infection
• Decision on stamping out • Supervision of cleaning and disinfection of premises, equipment and
other utensils when herd is depopulated
In total, lifting of restrictions require, from infected to officially free status, 4 negative surveys covering all animals over 6 months of age, with 30, 60, 90 and 90 days intervals
Bovine Brucellosis eradication programme Strategy 2014
RB51 Vaccination Programmes
Mainland: Montalegre and Vieira do Minho Ribeira de Pena Alentejo Region Cuba/Alvito (at Atentejo Region)
Autonomous Region of Azores: Terceira São Jorge São Miguel
Bovine Brucellosis eradication programme Strategy 2014
0.61%
0.56%
0.31% 0.27% 0.28%
0.15%
0.08% 0.06% 0.04% 0.04%
0.39% 0.43%
0.23% 0.22% 0.24%
0,00%
0,10%
0,20%
0,30%
0,40%
0,50%
0,60%
0,70%
2010 2011 2012(RÑOI)
2013(RÑOI)
2014(RÑOI)
Bovine Brucellosis (Continent & Azores) prevalence & incidence
Herd prevalence Animal prevalence Herd incidence
Years
Bovine Brucellosis eradication programme Results 2014
Nº Herds tested 31 515 Nº animals tested 785 303
Bovine Brucellosis eradication programme Results 2014
257 216 108 88 88
1355
713
518
328 352
0
200
400
600
800
1000
1200
1400
1600
2010 2011 2012(RÑOI)
2013(RÑOI)
2014(RÑOI)
Bovine Brucellosis (Continent & Azores) No positive herds & No positive animals
N.º positive herds N.º positive animals
Years Years
8 holdings with bacterial isolation (13%, n=62) 6 by B. abortus and 2 by B. melitensis
Regions Number of positive
herds
Number of new
positive herds
% positive herds
% new positive
herds
Norte 39 28 0.23 0.16
Centro 4 4 0.06 0.06
LVT 0 0 0 0.00
Alentejo 25 24 0.60 0.58
Açores 20 19 0.69 0.66
TOTAL 88 75 0.28 0.24
Bovine Brucellosis eradication programme Results 2014
72.2%
38.9% 33.3%
16.7% 11.1%
5.6%
0,0%
10,0%
20,0%
30,0%
40,0%
50,0%
60,0%
70,0%
80,0%
Direct contactwith ruminants
from other flocks
Introduction ofanimals
Common pasture Unknown / othersources
Recurrence Introd. animals/premovementtests not inaccordance
Epidemiological inquiries Source of infection
BB - 2014
Direct contact with ruminantsfrom other flocks
Introduction of animals
Common pasture
Unknown / other sources
Recurrence
Introd. animals/ premovementtests not in accordance
Bovine Brucellosis eradication programme Results 2014
Vaccination programme
Bovine Brucellosis eradication programme Results 2014
BB 2014
TOTAL Norte Alentejo Açores
N.º vaccinated herds 2 717 517 16 2 184
N.º vaccinated animals 25 194 1 309 1 238 22 647
Regions with favourable indicators of evolution of the disease - Additional measures for a better understanding of possible cases of
False Positive Serological Reactions - Herd depopulation
Ongoing work
Regions with more difficulties in decreasing prevalence - Thorough epidemiological investigation of outbreak´s - Animal movement control - Reinforcement of vaccination - Improve quality of bacteriological investigation
Bovine Brucellosis eradication programme Results 2014
PAFF Standing Committee Brussels, 7-8 July 2015
Implementation of
Sheep and Goats Brucellosis
Eradication Programme
in Portugal 2014
Regions Number of herds
Number of animals
Norte 16 741 419 284
Centro 27 959 551 923
LVT 7 106 184 539
Alentejo 7 926 1 065 055
Algarve 1 224 59 858
TOTAL 60 873 2 280 659
Approved by Decision 2013/722/UE
S&G Brucellosis eradication programme Characterization 2014
The Autonomous Region of Azores is Officially Free of Brucella melitensis Commission Decision 2003/644/CE, 17th January
Serological control is mandatory for animals over 6 months or over 3 months in the case of vaccinated with Rev1
In areas where 99.8% of S&G flocks are B3/B4, the representative fraction of the population over the age of 6 months to be tested is the following:
- all non-castrated male - all animals brought into the holding - reproductive females N.º of females at
reproductive age
N.º of females to
be tested
0 - 50 all
50 - 200 50
> 200 25% Measures in positive and infected flocks are identical to those applied in bovines
S&G Brucellosis eradication programme Strategy 2014
In regions with higher prevalence :
Trás-os-Montes (part of DSAVR Norte) Centro Region Algarve Region
S&G Brucellosis eradication programme Strategy 2014
REV1 Vaccination Programmes
Young animals 3-6 months of age
1.27% 1.35%
1.21% 1.10%
0.88%
0.41% 0.46% 0.32%
0.23% 0.23%
0.56% 0.61% 0.66% 0.68%
0.58%
0,00%
0,20%
0,40%
0,60%
0,80%
1,00%
1,20%
1,40%
1,60%
2010 2011 2012 2013 2014
Sheep & Goats brucellosis Incidence and prevalence
Herd prevalence Animal prevalence Herd incidence
S&G Brucellosis eradication programme Results 2014
98.2% coverage of herds under the programme 2 124 294 animals controlled
841 867 746 670
529
1.27% 1.35%
1.21% 1.10%
0.88%
0
100
200
300
400
500
600
700
800
900
1000
0,0%
0,2%
0,4%
0,6%
0,8%
1,0%
1,2%
1,4%
1,6%
2010 2011 2012 2013 2014
Sheep & Goats brucellosis N.º positive herds / herd prevalence
N.º positive herds Herd prevalence
years
S&G Brucellosis eradication programme Results 2014
62 holdings with Brucella melitensis isolation (15.3%, n=404)
Regions Number of positive herds
Number of new positive
herds
% positive herds
% new positive herds
Norte 374 228 2.28 1.39
Centro 35 35 0.13 0.13
LVT 53 36 0.81 0.55
Alentejo 44 39 0.57 0.51
Algarve 23 9 1.88 0.74
TOTAL 529 347 0.88 0.58
S&G Brucellosis eradication programme Results 2014
51.5%
33.3% 31.3%
26.3%
16.2%
7.1% 6.1%
0,0%
10,0%
20,0%
30,0%
40,0%
50,0%
60,0%
Epidemiological inqueries Source of infection
Bpr -2014
Introduction of animals
Direct contact with ruminants from other flocks
Common pasture
Recurrence
Unknown / other sources
Direct contact with flocks of the same owner
Transhumance
S&G Brucellosis eradication programme Results 2014
2014 TOTAL Norte Centro Alentejo Algarve
N.º vaccinated flocks 2 827 2 411 327 25 64
N.º vaccinated animals 32 379 25 203 5 530 833 813
S&G Brucellosis eradication programme Results 2014
Vaccination programme
Regions with favourable indicators of evolution of the disease - Additional measures for a better understanding of possible cases of False Positive Serological Reactions
Ongoing work
Regions with more difficulties in decreasing prevalence - Thorough epidemiological investigation of outbreaks - Animal movement control - Reinforce of vaccination - Investigate interference of vaccination in the serological diagnosis - Revision of quality of bacteriological investigation
S&G Brucellosis eradication programme
Direção Geralde Alimentaçãoe Veterinária
Year N.º of human cases
2010 86
2011 79
2012 47
2013 35
2014 52
Data from the Directorate-General of Health (DGS)
Brucellosis eradication programme
Notified human cases
Thank You for your attention