Implantable Infusion Pumps: Insights For Your Next Animal Dosing Study A webinar for scientists interested in the use of implantable pumps as an alternative to repetitive injections when administering compounds to laboratory animals. Presenter, José Gadea, clarifies misconceptions surrounding the use of implantable pumps and presents facts supporting their value in preclinical research.
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Implantable Infusion Pumps: Insights For Your Next Animal Dosing Study
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Implantable Infusion Pumps: Insights For Your Next Animal Dosing Study
A webinar for scientists interested in the use of implantable pumps as an alternative to repetitive injections when administering compounds to laboratory animals. Presenter, José Gadea, clarifies misconceptions surrounding the use of implantable pumps and presents facts supporting their value in preclinical research.
InsideScientific is an online educational environment designed for life science researchers. Our goal is to aid in
the sharing and distribution of scientific information regarding innovative technologies, protocols, research
FACT - ALZET pumps are capable of delivering homogeneous solutions with a viscosity of less than 100,000 cP or mPa s.
ALZET Pump: Drug Formulation
MYTH – Using nominal specifications for dose calculations
FACT – Use actual pumping rate and fill volume listed on the specifications sheet for each lot of pumps when making dose calculations Model 2002 (Lot #10196-08)
Nominal Actual
Release Rate 0.5 ml/hr 0.53 ml/hr
Fill Volume 200 ml 216 ml
Duration 14 days 16.1 days
ALZET Pump: Filling
• Use aseptic technique
• Weigh pump and flow moderator before filling
• Use the filling tube provided
• Fill the pumps with the curved end down
• Fill with the flow moderator removed
• A small amount of backpressure is normal
Expert tip: If you experience too much pressure, angle the needle slightly to allow air to escape, or insert and remove the flow moderator a few times to widen the opening.
ALZET Pump: Priming
Priming is essential when:
• Immediate pumping is required• A catheter is used with the pump• A viscous solution is delivered• The drug solution may have acute toxic effects
Priming ensures that the pumps deliver at their specified pumping rate at time of implantation. To prime, place the filled ALZET pumps into an aqueous solution at 37 C for a specified time (3-60 hours depending on pump model).
ALZET Pump: Routes of Administration
Subcutaneous Intraperitoneal
ALZET Pump: SC Implantation Procedure
The usual site for SC implantation in rats and mice is on the back, slightly posterior to the scapulae.
Procedure:• Anesthetize and shave the animal• Make a mid-scapular incision suitable for the pump• Create a subcutaneous pocket by blunt dissection using a hemostat• Insert the pump into the pocket, delivery portal first• Close the incision with wound clips
MYTH - ALZET pump implantation is too difficult
FACT – The SC implantation procedure can be performed in under a minute
Minimum Size for Implantation of ALZET Pumps
ALZET Models1003D, 1007D, 1002,
10042001D, 2001, 2002,
2004, 20062ML1, 2ML2, 2ML4
MICE
Subcutaneous 10 g 20 g N/A
Intraperitoneal 20 g N/A N/A
RATS
Subcutaneous 10 g 20 g 150 g
Intraperitoneal 20 g 150 g 300 g
Note: Estimates based on experience with Sprague Dawley rats and Swiss Webster mice.
ALZET Pump: Implantation
MYTH - ALZET pumps restrict animal movement
FACT – Rodents have very loose skin on the back. ALZET pumps are used in mice and rats that are used in various behavioral tests (I.e., open field, rotarod, Morris water task, swim test, etc.)
Intravenous
Brain cannulation
Targeted Delivery
• Blood vessels
• Central Nervous System
– Cerebral ventricles, brain tissue, Spinal Cord
• Peripheral nerves
• Various organs and tissues
– Tumor, bone, eye, ear, muscle, wound
ALZET Pump: Routes of Administration
ALZET Pump: Catheter Applications
1. To delay drug delivery
• Pump filled with drug
• Catheter filled with vehicle
2. To reduce drug waste
• Pump filled with vehicle
• Catheter filled with drug
3. To deliver incompatible solvents
1. Measurement of plasma levels
2. Measurement of residual volume
3. In vitro release rate testing
ALZET Pump: Verifying Delivery
MYTH – Weighing pumps at the end of the study to confirm delivery
FACT – The weight of a partially empty pump or cutting a pump is not a reliable means of verifying pump performance
iPRECIO Programmable Pump
24.8 mm
15.0 mm
H: 7.2 mm
Weight: 3.3 g
19.2 mm
H: 9.7 mm
Weight: 7.9 g
38.7 mm
SMP-300 SMP-200
iPRECIO Pump: Key Features
• Implantable
• Refillable
• Programmable
• Fully implantable in mice, rats and larger animals• SMP-200: >230 grams
• SMP-300: >22 grams
• Unrestrained dosing
• Group housing
• Reduced animal stress
iPRECIO Pump: Implantable
• In vivo refilling via percutaneous access
• Eliminate multiple surgeries
• Increasing dose studies
• Multiple drugs
• Poor drug stability/solubility
iPRECIO Pump: Refillable
MYTH – iPRECIO pumps can be reused
FACT – iPRECIO pumps are refillable, but not reusable
• Full control and flexibility over dosing protocols
• Set your own infusion protocol: start/stop time, flow rate, duration, etc
• Download infusion protocol to the pump via communication device• SMP-200: IR communication
• SMP-300: wireless communication
iPRECIO Pump: Programmable
iPRECIO Pump: Programmable
• Constant/variable dosing
• Delayed delivery
• Dose escalation
• Chronic bolus
• Accurate and reliable “Rotary Finger” mechanism (Pulse micro-motor)
• Microprocessor and associated circuitry for pump control (CPU, memory)
• Power source (battery)
• Fluidics (Reservoir, catheter, filling port)
• Pump programming and communication interface (software and base station)
iPRECIO Pump: Key Components
iPRECIO SMP-200
Bottom ViewTop View
Antenna: 50 mm titanium wire (0.1 mm OD) inside PU tubing (0.4mm OD)
Top ViewBottom View
iPRECIO SMP-300
iPRECIO Pump: Mechanism of Operation
Patented "Rotary Finger" mechanism
• A micro-motor slowly revolves in a clockwise direction turning the cam with its four projections.
• In each quarter rotation, a single cam projection sequentially pushes up each of the seven finger pins.
• This continuous cycle compresses the liquid filled tube, creating a peristaltic-like movement of the fluid.
• As the solution moves through the tube, it is expelled from the pump reservoir into the test subject.
Rotary Finger Mechanism
• Accuracy: +/- 5%
• Each pump calibrated at factory
iPRECIO® Management System IMS-200• Data communication Device UCD-200• USB cable• iPRECIO® Software Installation CD• iPRECIO® Users Manual• (2) AAA cell batteries
iPRECIO® Management System
iPRECIO® Management System IMS-300• Data communication Device UCD 300• LAN cable• iPRECIO® Software Installation CD• iPRECIO® Users Manual
iPRECIO Programming (SMP-200)
1. Enter study details• Name/ID
• Date
• User
• Animal: species/strain/age
• Route
• Duration
• Compound name and concentration
• Groups / # of animals
iPRECIO Programming (SMP-200)
2. Enter group details• Est. min and max animal
weight
• Drug concentration
• Select infusion mode/group
• Instant vs. delayed
• Select flow rate mode/group
• Constant vs. variable
iPRECIO Programming (SMP-200)
3. Enter animal details• Individual animals per group
• Animal ID
• Weight
• Sex
• Set infusion mode
• Start date/time
• End date/time
• Detect pump
iPRECIO Programming (SMP-200)
4. Pump information• Individual pump programming
• Step 1-10– Dose
– Duration
– Start/end time
– Flow rate
• Program pump
• Print report
iPRECIO Programming (SMP-300)
Monitor function:Allows the user to follow the infusion profile in detail. Refill dates/exchange dates and alarms are also managed and displayed here.
iPRECIO Pump: Key Specs Comparison
SMP-300 SMP-200
Size [L] x [W] x [H] (Weight/volume)
24.8 x 15.0 x 7.2 mm(3.3 g / 2.15 cc)
38.7 x 19.2 x 9.7mm(7.9 g / 7.20 cc)
Communication Wireless Infrared
Animal Species Mice or larger Rats or larger
Animal Size Suggested: 25 g (Min. 22 g) Suggested: 230 g (Min. 160 g)
Reservoir Volume 130 μL 900 μL
Flow Rate(Setting Resolution)
0.1 – 10.0 μL/hr (0.1 μL/hr)
0.1 – 30.0 μL/hr (0.1 μL/hr)
Duration Up to 47 days Up to 6 months
Battery Life: iPRECIO SMP-200
Flow Rate
Continuous InfusionTotal Infusion
Volume(Time) (hours)
1.0 μL/hr 6 months 4,328 hr 4.3 mL
8.5 μL/hr 1 month 669 hr 5.6 mL
19.0 μL/hr 1.8 weeks 307 hr 5.8 mL
30.0 μL/hr 1 week 196 hr 5.8 mL
Comm.Interval Every Minute Every 2 hrs Every 6 hrs Every 24 hrs None
Flow Rate (ul/hr)
Time (days) Time (days) Time (days) Time (days) Time (days)