DAKO IHC Staining Steps 1.Deparaffinization and Rehydration 2.Rinse with Buffer 3.Target Retrieval – Cool 4. Rinse in Tap Water and DW 5.Block Endogenous Peroxidase 6.Rinse with Buffer 7.Primary Antibody 8.Rinse with Buffer 9.Envision 10.Rinse with Buffer 11.DAB 12.Rinse with DW 13.Hematoxylin 14.Rinse with TW 15.Coverslip with Aqueous Resin
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DAKO IHC Staining Steps 1.Deparaffinization and Rehydration
2.Rinse with Buffer
3.Target Retrieval – Cool 4. Rinse in Tap Water and DW
5.Block Endogenous Peroxidase 6.Rinse with Buffer
7.Primary Antibody 8.Rinse with Buffer
9.Envision 10.Rinse with Buffer
11.DAB 12.Rinse with DW
13.Hematoxylin 14.Rinse with TW
15.Coverslip with Aqueous Resin
1.Deparaffinization and Rehydration
1. Xylene - 5 mins.2. Xylene - 5 mins.
3. Absolute ethanol -3 mins.4. Absolute ethanol - 3 mins
5. 95% ethanol - 3 mins.6. 95% ethanol - 3 mins.
7. Distilled water (30 seconds to 1 min.)
3.Target Retrieval
Total: 15 – 18 mins. plus 20 mins. cooling time = 35-38 mins)1. High - 5 mins.2. Medium High/Low - 5 mins.3. Medium - 5 mins.4. Medium - 3 mins.5. Cooling time - 20 mins.