Result “Anbody seems to specifically label βIII-tubulin, highlighng neurites of the cultured cells.“ - J. Heller, University Col- lege London. Cell Line: Rat dissociated cortical cul- ture (14 DIV) Method of validaon: Immunocytochemistry Primary Anbody: STJ97123 An-β III Tubulin anbody Secondary Anbody Goat an-rabbit AlexaFluor 647 (1:500) Diluon rao: 1:100 Protocol Treatment of materials: Corcal cells were fixed with 3.7% PFA for 12 min at 37°C. Membrane wash: PBS 2x 5 min, gently rocking at RT ; Quenching: 0.1% NaBH4 in PBS for 7 min, gently rocking at RT ; Membrane wash: PBS 1x 2 and 3x 5 min, gently rocking at RT ; Permeabilizaon: PBS supplemented with 0.1% triton X-100 (PBS-T) for 10 min, gently rocking at RT ; Membrane wash: PBS 1x 2 and 3x 5 min, gently rocking at RT ; Blocking: blocking buffer (PBS supplemented with 3% BSA) for 30 min, gently rocking at RT ; Primary anbody probing: PBS supplemented with 0.5% BSA overnight at 4°C ; Membrane wash: PBS 1x 2 and 3x 5 min, gently rocking at RT ; Secondary anbody probing: Fuorescently-Labelled secondary anbodies diluted in PBS for 1 h, gently rocking at RT; Membrane wash: PBS 5x 10 min, gently rocking at RT; Fixaon: 4% PFA in PBS for 10 min at RT ; Membrane wash: PBS 3x 10 min, gently rocking at RT ; Visualizaon: Imaging in PBS. Anbody Customer Review: STJ97123 An-β III Tubulin Anbody Anbody Specificity: Anbody Rang: Fig.1 The anbody labelling reveals neurites of cultured cells.