2 Immuno Tools Your source for innovations .com Hotline 04 interbiotech@ Interchim provides more than 100 000 primary antibodies and all secondary and accessory reagents to perform your immunodetections in ELISA, Blotting, FCM or microscopy techniques. This includes : » Our high quality II antibodies labeled by HRP, Alkaline Phosphatase, Biotin and our great FluoProbes dyes » Fluograde microplates (FPlyte) » Ultimate sensitivity HRP substrates (TMB plus) » Remarkable saturating agents (Seablock).
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Hotline 04 70 03 73 06interbiotech@ interchim.com
Hotline 04 70 03 73 06interbiotech@ interchim.com
Interchim provides more than 100 000 primary antibodies and all secondary and accessory reagents to perform your immunodetections in ELISA, Blotting, FCM or microscopy techniques. This includes :» Our high quality II antibodies labeled by HRP, Alkaline Phosphatase, Biotin and our great FluoProbes dyes» Fluograde microplates (FPlyte)» Ultimate sensitivity HRP substrates (TMB plus)» Remarkable saturating agents (Seablock).
Hotline 04 70 03 73 06interbiotech@ interchim.com Prim'Ab specific topics
Introduction
See Prim'Ab full table in chap.E
The use of primary antibodies (Prim’Ab) as research tools is, in many ways, responsible for much of the progress seen in modern biological research. These antibodies, specific of target antigens, are a first step to probes samples in various detection. They can also be used to immobilize antigens on supports. Secondary antibodies are used in a second step to detect and amplify Prim’Ab signal by colour or light emission as fluorescence or chemiluminescence.
INTERCHIM is proud to offer one of the largest choice of high quality primary antibodies (more than 100 000 items) from the most famost manufacturers all over the world.
The following pages present a list of :» Interchim’s reliable suppliers for primary antibodies» Interesting and unique antibody technical features (such as Rabbit monoclonals...)» Anti-tags and labels antibodies» Loading control antibodies» Phosphospecific antibodies
One of the earliest use for antibodies, came in the early part of the 20th Century when Karl Lands-teiner develop a serological test for identifying different blood groups (ABO system for typing blood). Today antibodies are used as versatile reagents for an extremely wide range of assays, in technics as varied as screening phage libraries, biosensors, affinity purifications, and conventional immunoassays including ELISA, blotting, flow cytometry, agglutinations...Because of these multiple uses, a wide range of antibodies is needed (regarding antigen-specificity, binding affinity, and format/purity). Fortunately, a large number of companies now provides antibodies for researchers. This means that each individual lab no longer has to go through the hassle of generating, purifying and testing their own antibodies. However it is often difficult to find out the antibody companies, and the best supplier.
Some History...
A large selection of our antibodies and antigens are listed in chapter E. This table (by alphanumerical order of specificities) displays key information: species cross reactivity, host and quantity, compatible immunotechniques.
These listings cannot be exhaustive as there are too many items available. Please visit our website and Prim’Ab search engine to get the most updated list.
Prim’Ab search engineOver 100 000 primary antibodies available online with our powerful Prim'Ab search. Prim'Ab search offers an easy way to compare antibodies at a glence by displaying key datas, such as species reactivity, tested applications, epitope...
If the antibody your are looking for is not available on the market, keep in mind our custom services : Custom Rabbit and Chicken polyclonals, as well as RABBIT MONOCLONALS or GENE IMMUNIZATION (see our “Custom Services” chapter).
Thanks to : » its hudge database» its excellent technical support» and its ability to provide all associated products (secondary, substate, kits…)
INTERCHIM is your reliable supplier for primary antibodies.
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Hotline 04 70 03 73 06interbiotech@ interchim.com
Hotline 04 70 03 73 06interbiotech@ interchim.comPrim'Ab specific topics
Suppliers
Interchim Prim’Ab suppliers are famous labs around the world, each of them with their own speciality.
■ Novus Biologicals» Wide range (almost 15000 with hundreds new each month)» Very well referenced in litterature» More and more sample sizes available» HIF (Hypoxia Inducible Factor) specialist
■ LifeSpan Biosciences» The IHC specialist » Wide range (over 35000 items with hundreds new each month)
LifeSpan,the IHC specialistSee our "Custom Services" chapter (p. xx) for more details.
■ Abnova» One of the largest antibody manufacturer in the world (over 20 000 available with hundreds new each month)» Over 50% from the catalogue is unique» Mouse polyclonals, monoclonals, and MaxPab®
MaxPab® antibodies :a new collection of polyclonal antibodies generated from annotated, sequence verified full-length proteins.• Power, precision, performance• Silencing efficiency assesment (most RNAi are available)• Both conformational and linear epitopes are recognized
■ Abgent» Sample sizes available for each catalog item» Core range focus on post-translationnal modification» Unique range in Autophagy area» Great antibody sampler kits for each research topic
Antibody sampler kitsSampler kits offer an affordable way to purchase multiple validated antibodies.
■ Spring Bioscience» Sample sizes available for each catalog item» Rabbit monoclonal antibodies (RabMabs)» Wide range (mainly focus on IHC)
Rabbit Monoclonal antibodies (RabMabs)RabMabs combine the high affinity of rabbit antibodies and very high monoclonal specificity.
■ Fitzgerald Industries International and Research Diagnostics Inc (RDI now parts of Fitzgerald)
» Wide range with a large part dedicated to Infectious diseases» Antigens
Meridian LifeScience now merges 4 brands :• Biodesign International• OEM concept• Viral antigens• Meridian Biologics
■ Genway Biotech » Chicken IgY specialist
IgY Chicken antibodiesIgY antibodies have multiple advantages over IgG antibodies, including higher yields, lower backgrounds, higher specificity, stronger avidity, and applicability to many immmunoassays.
■ And many othersInterchim works with over 50 antibodies suppliers, including :
Hotline 04 70 03 73 06interbiotech@ interchim.com Prim'Ab specific topics
Interesting topics and unique technical features
■ MaxPab® antibodies (over 9000 MaxPab® antibodies available and hundreds new each month)MaxPab® is a new collection of polyclonal antibodies generated from annotated, sequence verified full-length proteins. Each MaxPab® antibody is validated with a full-length human protein expressed from a mammalian transfected lysate, demonstrating high reactivity, sensitivity and specificity.
» Power, precision, performance» Silencing efficiency assesment (most RNAi are available)» Both conformational and linear epitopes are recognized» More epitopes recognized = chance to cross-react with other species» Broad application range
ORC6L MaxPab® polyclonal antibodyImmunoperoxidase of purified MaxPab® antibody to ORC6L on formalin- fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml]
ING2 MaxPab® polyclonal antibodyImmunofluorescence of MaxPab® antibody to ING2 on HeLa cell .
■ Antibody sampler kitsSampler kits offer an affordable way to purchase multiple validated antibodies from Abgent's comprehensive antibody product lines. Assemble a collection of antibodies to your pathway of interest, or economically screen for the antibody that is perfect for your application.
Name P/N : QtyAcetylation Sampler Kit DS8960 5 antibodies or more (20ug each) to choose among the acetylation listApoptosis Sampler Kit KN1370 5 antibodies or more (20ug each) to choose among the apoptosis listAutophagy Sampler Kit KN1380 5 antibodies or more (20ug each) to choose among the autophagy listMethylation Sampler Kit DS8970 5 antibodies or more (20ug each) to choose among the methylation listNeurosciences Sampler Kit DS8980 5 antibodies or more (20ug each) to choose among the neurosciences listPhosphospecific Sampler Kit DS8910 5 antibodies or more (20ug each) to choose among the phosphospecific listStem Cell Sampler Kit DS8990 5 antibodies or more (20ug each) to choose among the stem cell listSumoylation Sampler Kit DS9000 5 antibodies or more (20ug each) to choose among the sumoylation listUbiquitination Sampler Kit DS9020 5 antibodies or more (20ug each) to choose among the ubiquitination list
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Interesting topics and unique technical features
■ Rabbit monoclonal antibodies (RabMabs)RabMAbs combine the high affinity of rabbit antibodies and very high monoclonal specificity, previously only seen with mouse antibodies.These combined advantages account for the superior performance of RabMAbs in IHC.Technically RabMAbs provide superior performance :
» Affinity and specificity produce superior quality staining. (These can be markedly demonstrated with clone SP1 for Estrogen Receptor). Low expressing tissues in particular show superb clarity of staining.» High sensitivity ensures perfect staining and economical use of antibody at higher working dilutions.» The superior diagnostic capability of RabMAbs can be demonstrated using clone SP4 for Cyclin D1.» RabMAbs are ideally suited and greatly simplify double staining methods in IHC.» For research purposes RabMAbs eliminate most of the problems associated using mouse antibodies on mouse tissues.
Why Rabbit Monoclonals ?Rabbits are known to have more complex immune system than mice. They produce a high affinity polyclonal response to challenge by immunogens. The complexity of clones produced and their high affinity enables selection of the most appropriate antibody, combining high sensitivity and specificity.Advanced cloning methods have lead to the successful fusion of rabbit-B lymphocytes and a rabbit plasmacytoma cell line (240E) to produce very stable rabbit-rabbit hybridoma to guarantee continuity and quality of RabMAb supply.
References :1. Rossi S. et al "Rabbit Monoclonal Antibodies. A comparative study between a novel category of immunoreagents and the corresponding mouse monoclonal antibodies." Am J Clin Pathol 2005 Aug 124(2):292-3022. Cano G., et al." Estimation of hormone receptor status in fine needle aspirates and paraffin-embedded sections from breast cancer using the novel rabbit monoclonal antibodies SP1 and SP2", Diagn Cytopathol,2003 Oct ; 29(4):207-113. Cheuk W., et al "Consistent Immunostaining for Cyclin D1 can be achieved on a routin basis using newly available Rabbit monoclonal Antibody", .Am J Surg Pathol 2004 June 28(6):801-07
Selected list of rabbit monoclonal antibodies (full list includes about 700 antibodies and is available on our website : Prim’Ab search).
Hotline 04 70 03 73 06interbiotech@ interchim.com Prim'Ab specific topics
Interesting topics and unique technical features
■ Chicken IgY antibodiesIgY antibodies have multiple advantages over IgG antibodies, including higher yields, lower backgrounds, higher specificity, stronger avidity, and applicability to many immunoassays.
Despite the overall similarities between IgY and conventionally used IgG antibodies, there are some profound differences in their chemical structures and biological features. IgY has a larger Fc region and a shorter hinge region than IgG. The IgY Fc region also has an additional pair of carbohydrate chains with unique sugar residues, important for highly efficient covalent coupling to solid surfaces such as microbeads or microarrays.
The affinity maturation process of IgY is via gene conversion, different from that of IgG via somatic mutation.
The phylogenetic distance between birds and mammals makes the chicken immune system often far more responsive to mammalian protein antigens thus generating highly reactive antibodies, particularly targeted to conserved proteins against which IgGs are difficult or impossible to raise.
The IgY production process is non-invasive and accumulative from collecting eggs, in contrast to that of IgG which requires bleeding and collecting sera from immunized animals.
The productivity of IgY is also superior, since one egg can generate about 100 mg of total IgY, about the yield of 50-100 mice.
» Polyclonal IgY antibodies have been shown to be suitable for all the conventional immunoassays such as Western blot, immunoprecipitation, immunohistochemistry, cell sorting, cell/tissue staining, etc. The quality of these assays using IgY antibodies is either comparable to or better than using conventional IgG antibodies.» IgY antibodies have also been shown to be very useful for cell or tissue immuno-staining (Immunohistochemistry and Immunocytochemistry). Both types of applications can implement high-throughput screening via tissue or cell array methodology. IgY antibodies are particularly more suitable for array (chip), nano-bar, or microbead applications, due to their higher stability, lower cross-reactivity, stronger binding (avidity), and greater detection sensitivity than those of IgG antibodies against the same antigens. Each of these features has been confirmed through side-by-side comparisons of the two types of antibodies under identical conditions.
A large selection of our antibodies and antigens are listed in chapter E. This table (by alphanumerical order of specificities) displays key information : species cross reactivity, host and quantity, compatible immunotechniques.
Advantages of IgY over IgGComparison IgY IgGAnimal Chicken of Avian mammalSource Egg Yolk PlasmaAvidity Hight ModerateQuantity Large LimitedCross Reactivity Low HightIsolation Simple ExpensiveStability Good GoodProductivity High/Fast Low/Slox
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■ Loading Control AntibodiesTarget Host Species cross-reactivities Tested applications Size P/N :Actin, cytoskeletal Rabbit Hu, Ms WB, IP 0.1 ml BY4270Actin, cytoskeletal Rabbit Hu, Ms WB, IP 0.1 ml BY4272Actin, cytoskeletal SuperNovus pack Rabbit Hu, Ms WB, IP 0.1 ml KU4770G6PD Rabbit Hu, Ms WB, IP 0.1 ml AW6700G6PD, agarose immobilized Rabbit Hu, Ms IP 0.1 ml GP8250GAPDH Rabbit Hu, Ms WB 0.1 ml KY4162GAPDH Rabbit Hu, Ms WB 0.1 ml KY4163GAPDH Rabbit Hu, Ms WB 0.1 ml KY4161GAPDH Rabbit Hu WB 0.1 ml KY4164GAPDH Rabbit Ms WB 0.1 ml KY4160Histone H4 Rabbit Hu WB, IP 0.1 ml KW0560Histone H4 Rabbit Hu WB, IP 0.1 ml KW0561Tubulin beta Rabbit Hu, (Ms, Rt, Ck, Pg...) WB 0.1 ml KZ4690
Non-exhaustive list. See Prim’Ab table in chapter E or the Prim’Ab search engine at www.interchim.com for more antibodies.
Légende :NP : means Non-Phosphospecific match-paired antibody is available ; BP : means Blocking Peptide is available
Prim'Ab specific topicsPhosphospecific antibodies
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Epitope Tag Antibodies
Tag Host Tag sequenceP/N :
Unconj. HRP FITC Other*6-His Chicken HHHHHH X MD1790 KX2390 X6-His Mouse Mab HHHHHH GK2290 OA2180 OA2190 OA2170 (B)6-His Rabbit HHHHHH MD7681 X OG2950 XAU1 Goat DTYRYI KW3521 KX0160 KX0170 GP8180 (A)AU1 Rabbit DTYRYI KU6151 X X XAU5 Goat TDFYLK KU5902 KX0090 KY2270 GP8310 (A)AU5 Rabbit TDFYLK KU5311 X X XBTag Rabbit QYPALT CF5920 X X XC-myc Chicken AW4270 ME6630 MG1060C-myc Goat AW4520 AW4560 AW4550 AW4540 (A)
C-myc Mouse Mab AEEQKLISEEDLLRKRREQLKHKLE GK2260 X OA1800 X
C-myc Rabbit ME6930(monoclonal)
AW6490 (polyclonal)
DYKDDDDK Mouse Mab DYKDDDDK OB0000 OB0010 OB0020 (B)DYKDDDDK Rabbit DYKDDDDK CF5910 X X XECS Chicken DDDDK AW4290 X X XECS Goat DDDDK AW4570 AW4610 AW4600 AW4590 (A)ECS Rabbit DDDDK AW9660 AW6620 E-Tag Goat GAPVPYPDPLEPR KU6842 KZ3760 KZ1170 KU5230 (A)E-Tag Rabbit GAPVPYPDPLEPR KU6672 KU6671 X XGalactosidase beta Rabbit GS8760 GS8800 GS8790 GS8770 (A)GFP Mouse Mab GK2280 X X X
* A = Agarose Immobilized ; B = Biotin
Western blot analysis of anti-GFP Mab (Cat. # GK2280) using purified GFP, YFP and BFP proteins expressed in bacteria : Both GFP (Lanes 1-3) and YFP (Lanes 4-6) but not BFP (data not shown) were detected using the purified Mab.
Immuno-fluorescence for GFP Tag Antibody (Cat # GK2280). Antibody target is a YFP tagged protein (the inner membrane complex) of Toxoplasma gondii, which localizes around the exterior of the parasite. Brighter parasites represent actively dividing cells and darker ones are not actively dividing.Data courtesy of Seth Robertson, Boston College.
Hotline 04 70 03 73 06interbiotech@ interchim.com Prim'Ab specific topics
Epitope Tag Antibodies
HA-tagged recombinant protein was expressed in COS7 cells. 15 ug of cell lysate was loaded onto a gel to test the HA tag antibody (Cat.# GK2270).
Western blot analysis of anti-HA Tag Antibody (Cat.# GK2270). in 12tag recombinant protein. 12tag recombinant protein was detected using the purified Mab.
Tag Host Tag sequenceP/N :
Unconj. HRP FITC Other*Glu-Glu Chicken EYMPME AW4310 X X XGlu-Glu Goat EYMPME AW4620 AW4660 AW4650 AW4640 (A)Glu-Glu Rabbit EYMPME AW9680 AW6920 X XGST Goat AW4670 AW4700 AW4710 AW4690 (A)
Mouse Mab GK2300GST Rabbit GK4380 AW6940 X XHA Chicken YPYDVPDYA AW4360 X X XHA Goat YPYDVPDYA AW4720 AW4760 AW4750 AW4740 (A)HA Mouse Mab YPYDVPDYA GK2270 X X XHA Rabbit YPYDVPDYA S42812 AW6950 X XHSV Goat SQPELAPEDPED KU6591 KZ3110 KY6380 KX1860 (A)HSV Rabbit SQPELAPEDPED KU7061 KZ0280 X XKT3 Goat KPPTPPPEPET KY4042 KY4041 KY4040 GP8410 (A)KT3 Rabbit KPPTPPPEPET KZ3210 X X XLuciferase Rabbit N91750 KU5200 KU5250 XMBP Mouse Mab GU8980 GU9010 X XProtein C Rabbit CEDQVDPRLIDGK CF5930 X X XS-Tag Goat KETAAAKFERQHMDS KU6622 KZ3810 KZ3590 KU5821 (A)S-Tag Rabbit KETAAAKFERQHMDS KU7622 KZ4290 X XT7 Chicken MASMTGGQQMG AW4370 X X XT7 Goat MASMTGGQQMG AW4810 AW4850 AW4840 AW4830 (A)T7 Rabbit MASMTGGQQMG AW9780 AW8250 X XV5 Chicken GKPIPNPLLGLDST AW4390 X X XV5 Goat GKPIPNPLLGLDST AW4860 AW4900 AW4890 AW4880 (A)V5 Rabbit GKPIPNPLLGLDST KM1760 AW8340 X XVSV-g Goat YTDIEMNRLGK KX5690 KW7700 KW4110 GP8160 (A)VSV-g Rabbit YTDIEMNRLGK AK9461 X X X