Image Processing Using ImageJ Norbert Vischer Centre for Advanced Microscopy University of Amsterdam
Image Processing Part 1
Feb 22, 2016
Image Processing Part 1. Image Processing Using ImageJ. Norbert Vischer. Centre for Advanced Microscopy University of Amsterdam. From Images to Results. From Images to Results. Image processing Image Analysis Data Organization. ImageJ Menus. Image as a matrix (CCD). - PowerPoint PPT Presentation
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Image Processing Using ImageJ
Norbert Vischer
Centre for Advanced MicroscopyUniversity of Amsterdam
Image processing Image AnalysisData Organization
From Images to Results
Full well capacity:15 000 electrons
Image as a matrix
4 MP camera:
1 MPixel red2 MPixel green1 MPixel blue
4 MP color image
4 MPixel red4 MPixel green4 MPixel blue
Missing fields are interpolated
Color images
File types
Norbert Vischer
This is my Comment
TIFF (Tag Image File Format) : 8-bit 16 bit, 32bit, RGB,
uncompressed in ImageJ
JPEG / JFIF: Lossy Compression, 1:25
GIF (Graphics Interchange Format) : 8 bit Graphics, Animation,
lossless
PNG (Portable Network Graphics): RGB Graphics, Transparency,
lossless, superior to GIF, for screenshots
DICOM
FITS
File types
- 8 Bit- 16 Bit- RGB color-32 Bit Floating point----------------------------- (Hyper-)Stacks- Metadata- Spatial calibration-Density calibration
- Only uncompressed TIFF in ImageJ
TIFF is the most versatile format
TIFF: A File system within a file
JPEG Compression / Decompression
JPEG Artifacts
8 * 8 cell
Bit depth of image types
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