iGEM @ Imperial
Jan 05, 2016
iGEM @ Imperial. Only three ideas made the cut. Bio-Clock (Re-defining Time). Pulse of AHL moves along a gutter of medium Cells fluoresce when activated Refractory period The time period is controlled by the radius gutter width cell density. Bio-Memory. - PowerPoint PPT Presentation
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iGEM @ ImperialiGEM @ Imperial
Bio Hard Drive
Cell Networking
Bacterial balloon
Biological to Electrical Interface
Bio-Clock
Bio Calculator
Remote Controlled
Bacteria
Crazy Ideas
Only three ideas Only three ideas made the cut.made the cut.
Bio-Clock Bio-Clock (Re-defining Time)(Re-defining Time)
• Pulse of AHL moves along a gutter of medium
• Cells fluoresce when activated
•Refractory period
•The time period is controlled by
•the radius
•gutter width
•cell density
Bio-MemoryBio-Memory
•Data stored in bacteria written using a green laser
•Data read using a UV laser + fluorimeter
•Cells either 1 (RFP) or 0 (no RFP)
•Data stored in switch
Very High Compression due to small size of bacteria
The OscillatorThe Oscillator
Changing Concentration of AHL
Culture Wide Culture Wide oscillations of AHLoscillations of AHL
Frequency must Frequency must be tuned easilybe tuned easily
Oscillations must Oscillations must be stablebe stable
Lotka-Voltarra Model output
Predator Prey DynamicsPredator Prey Dynamics
Simply Make a Bio-
chemical system
that can do this.
DesignDesign
A
B
A B
• Positive Feedback of A
• AB Induces production of more B
• Both A and B are used to make AB
Two Cell SystemTwo Cell SystemTwo independent populations of CellsTwo independent populations of Cells
These cells These cells do notdo not kill each other kill each other
AB
Altering the initial ratios of these cells will Altering the initial ratios of these cells will alter the frequency of oscillationsalter the frequency of oscillations
Design Cell1 (Prey)Design Cell1 (Prey)
Prey cell must produce molecule A exponentiallyPrey cell must produce molecule A exponentially
Pc
Plux
Lux R
Lux I
Pc is always on
Lux R is produced which detects molecule A
Then initiates transcription at Plux
Which Produces More AA
A
A
A
Design (isolated models)Design (isolated models)
Modelling of Prey Cell (system 1)Modelling of Prey Cell (system 1)
Design (isolated models)Design (isolated models)
Modelling of Prey Cell (system 1)Modelling of Prey Cell (system 1)
DesignDesign
The Predator CellThe Predator Cell
The role of a predator is to reduce the prey numbers as a function of the predator population numbers.
PredatorDetect Prey Population Size
Reduce Prey Population Size
DesignDesign
Plux Lux R aiiA
The Predator CellThe Predator Cell
Detects Prey Population Size
Reduces Prey Population Size
DesignDesign
Plux Lux R aiiA
The Predator CellThe Predator Cell A
A
LuxR
LuxR A
aiiA
Design (Entire System)Design (Entire System)
Extra cellular pool of A (HSL) (this should oscillate)
Diffusion
Plux
Lux R aiiA
The Predator CellThe Predator CellA
A
LuxR
LuxR
A
aiiA
A
Pc
Plux
Lux R
Lux I
A
The Prey Cell
ModellingModelling
Tom’s Tom’s MonsterMonster
ModellingModelling
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