IF1IF2 DF1DF2DF3DF4 MS LASER microscope (observation) objectives (observation, focus) a) b)b) Figure 1.

IF1IF2 DF1DF2DF3DF4

MS

LASER

microscope (observation)

objectives (observation, focus)

a)

b)

Figure 1

CCD camera(500/200 * 5= X12.5)

f=500mm condenser lens

Coaxial epi-illumination

355nm LASER

X5 infinity-corrected objectives(NA=0.13, WD=35mm)Spot diameter R=1.6mm （ theoretical ）Focal depth 263≒ mm(R=20mm)

Dichroic mirror

Half mirror

a)

b)

Figure 2c)

Figure 3

Peak picking

.

.

.

.

.

MS slice images

Average spectrum viewer

MS slice viewer

TIC image viewer

a)

b)

Figure 4

A B

C D

Figure 5

A

B C

D E

Figure 6

273.03904

382.82639

667.34340 795.53110 931.657030.5

1.0

1.5

2.0

2.5

5x10

347.00358441.29799

667.34340

795.53110

931.65703

1.0

1.5

2.0

2.5

4x10

200 300 400 500 600 700 800 900 m/z

321.04874

321.07601

321.09105 321.12783

321.20400

0.80

0.85

0.90

0.95

1.00

4x10

321.09463321.18384

321.21053

2

4

6

4x10

321.04895

321.09390 321.13211

0.0

0.2

0.4

0.6

0.8

1.05x10

321.04 321.06 321.08 321.10 321.12 321.14 321.16 321.18 321.20 321.22 m/z

C

B

A

D

Figure 7

E

Figure 8

Figure 9

a)

b)

Figure 10

a)

b)

c)

A

A

A

B

B

B

C

C

C

LDI MALDI/DHBA

MALDI/CHCA

246E(m/z)=495.401

771E(m/z)=490.752

86E(m/z)=336.988

132E(m/z)=536.278

2652E(m/z)=373.679

2242E(m/z)=244.300

868E(m/z)=475.115

Figure 11