Identification of possible biological stains for differential staining of Normal and Malignant tumour tissue Nor Hayati Othman, Pathology dept, PPSP [email protected]
Dec 21, 2015
Identification of possible biological stains for differential
staining of Normal and Malignant tumour tissue
Nor Hayati Othman,
Pathology dept, PPSP
Objectives
• To search for suitable natural stains derived from national plants of Malaysia
• To determine the differential staining of the new stain on human normal and malignant tumour tissue
Normal Vs cancer cell
Normal celln:c 1:4-8smooth nuclear membraneno nucleus
Malignant celln:cmore hyperchromatic [more basic]nuclear membrane coarsechromatic coarse, stippledmitosispleomorphic
Cytoplasm - ± opaque
Those are easy cases!!
• Subtle changes in cell require further tests
• Immunohistochemsitry– We stain the proteins found in cells eg muco-
proteins, keratins, vimentins, osteobilins, neurofilaments etc
– Every protein has different characteristics and could be stained
The stain should be
• Permanent
• Able to differentiate acidic nucleus from basic cytoplasm
• Able to pick up loss of desmosomes when normal epithelial cell become malignant
• Able to detect changes in nucleus when cells become malignant
How to verify the stains?
• 100 Normal and 100 Epithelial Malignant human tissues obtained from the archives of Pathology laboratory.
• The ability to differentiate will be scored and statistically analysed