IACUC Updates / Health and Safety Training Elaine Major, Director of Institutional Compliance 215 Wannalancit, ext. 3452 Dr. Scott Perkins, VMD, MPH, ACLAM Cell 617-947-5770 or email [email protected]A my Finneral, Animal Research Compliance Manager 618 Olsen, ext. 4698
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IACUC Updates /Health and Safety Training
Elaine Major, Director of Institutional Compliance215 Wannalancit, ext. 3452
Dr. Scott Perkins, VMD, MPH, ACLAMCell 617-947-5770 or email [email protected]
Amy Finneral, Animal Research Compliance Manager618 Olsen, ext. 4698
OverviewUpdates
Dr. Peter Gaines, IACUC Chair in Jan. 2013! Updated Policies and Procedures on webGuide for the Care and Use of Laboratory Animals: Eighth Edition
(Guide), adopted by NIH 1-1-12 http://grants.nih.gov/grants/olaw/Guide-for-the-care-and-use-of-Laboratory-animals.pdf
Facility updates Per diems- estimate 10% increase, possibly billed through CRF Occupational Health Evaluation REQUIRED Schedule all activities in facility (esp. procedure room)
Risks from Working with Animals and Zoonoses
Policy and Procedure UpdatesCore Research Facility process for billingUpdated Information on website
Forms - http://www.uml.edu/Research/OIC/animal-use/forms.aspx
Guidelines and Policies Murine Tail Biopsy Physical Restraint and Immobilization Use of Freund’s Adjuvant
Protocol Process!!! Protocols approval PRIOR to animal use
To assure NIH OLAW in compliance with PHS and USDA requirements
AV available to assist and provide recommendations Include options to provide flexibilityProcure all supplies before initiating project Review approved protocol with all research personnelReview protocol often and update with amendments If not enough detail, will not go on meeting agenda
Facility RequirementsTraining Required
CITI training (regulatory information), every 3 years Occupational Health Clearance, annually EHS Lab Safety Training, annually Animal Handling Training Session, as needed Research specific activities (PI), as needed
No eating or drinking in facilityNo cell phone use (photography policy)PPE requiredSchedule activities with facility manager
Facility Reminders (cont.)
Use controlled substance log as necessaryNO transport of animals outside facility without
specific approval (Refer to Animal Transport Policy)Report ANY incident
Include description of what occurred, reasons why you think it occurred, what was done to alleviate pain/distress for the animal, suggested revisions to address and prevent the problem from recurring
OIC investigates and files reports with OLAW, as appropriate
IACUC can stop activities if necessary
Supplies OIC provides:
Cages (unless special) Regular food and bedding PPE Euthanasia supplies Supplies for facility training Basic housekeeping supplies
Researchers provide: All needles, syringes, surgical equipment, etc. Drugs/materials as noted on protocol Other materials specific to research activity
Visitor Policy
• Submit visit requests to OIC with justification for approval
• Generally not approved to see how animal research is conducted
• Visitors must verify that they have not been in any other animal facility on the day of the visit
• Visitors must wear appropriate PPE
Disposal of Dead Animals
• For animals found dead, update census information on cage card and review protocol to follow procedures for carcasses
• PI is notified (referencing protocol number and cage number or cage card information) to determine if necropsy desired
• If no handling instructions or PI is unavailable, animal is placed in a plastic bag and put in designated freezer
• For USDA regulated species, record Found Dead (FD) is noted in the animal's medical record
• ORS Manager contacts EH&S for pickup and disposal of carcasses when the freezer is full.
Working Reminders
Schedule all activities in facilityBring all research suppliesWear protective clothing (for you and animals!)
Gloves, shoe covers, lab coat, mask, goggles Dispose of used PPE in facility
Report ANY injury from an animalReport ANY incident affecting an animalClean up work area and wash hands before
leavingDispose of sharps and materials appropriately
Reporting ConcernsDocument concern or incident asap
Date, time, and location incident occurred Describe what happened Include names of personnel involved Outline actions to prevent re-occurrence Notify OIC and AV immediately!
For reporting any general concerns Email (Amy Finneral or Elaine Major) Phone (Anonymous Hotline x3100) Intercampus mail
For Success with the IACUC Provide enough detail in your protocolFollow protocol procedures as approvedAll personnel must complete trainingFollow the protocol and submit amendments if
necessaryUnderstand risks and how to minimize themBe respectful- may not agree but need to follow AV and
IACUC’s directives to ensure UML is in complianceUse the resources provided by the AV
Risks and Precautions for Work with Animals
LOW risk if follow precautionsEXTREMELY LOW risk of disease using
animals from reputable sources Immunization awareness
Tetanus required, Hepatitis B recommended
Occupational Health Clearance Baseline Annual review
Occupational Health Program
Essential part of the overall Program of Animal Care and Use at UML
Required for anyone working with animals or entering facility
Goal to identify personnel with animal allergies and determine PPE or equipment available to protect themComplete Baseline Health History QuestionnaireEmail to [email protected] or Fax with Cover Sheet to All One Health, Attn: Jeanne
Cotter, (781) 938-4686Reviewed by a health professional and clearance sent to EHS
and the Facility Manager before allowed access to the facility
Common Exposure Routes
Aerosol (inhalation) Ex. gases, centrifuging, flaming, shaking, pouring, etc.
Ingestion (swallowing) Ex. Water or food with Salmonella or Giardia
Absorption (skin, mucus membranes, open wounds) Ex. Material splashed on skin or open wound exposed directly
to animal or materials used
Skin penetrated (punctures) Ex. Accidental needle sticks, cuts, or bites
Common Hazards
Injuries Bites, scratches, needle sticks Repetitive motions Working with equipment
AllergiesAirborne contaminantsZoonosesBloodborne pathogens (biohazards) Biological and Chemical agents
If Injured...Notify your supervisorThoroughly wash site with soap and waterReport any bite or scratch
Flu like symptoms w/in several days after bite
Seek medical attention as necessary Call ext. 2911 if serious or go to an ERPrevention: Complete animal handling training
and use proper restraint techniques
AllergiesAllergies affect ~30% of all workers
Caused by proteins from a variety of fluids/tissues from many animal species
AnaphylaxisAirway problem due to severe allergic reactionLife threatening and can develop quicklySymptoms:
Hives: red, itchy, raised blotches on the skin Itchy watery eyes, runny nose Increased heart rate Wheezing, stridor, coughing Nausea & vomiting Swelling of the face
Zoonoses
Disease transmitted from animals to humans viruses, bacteria, fungi, protozoa internal and external parasites
Very rare in commercially bred animals and sporadicPrevention: use PPE, get animals from reputable
vendors, personal hygiene, avoid bedding and contaminated water, monitor animals for infection
Recognize Zoonoses Risk Factors
Learn about animals you work withUnderstand mode of transmission for
infectious agentsAssess potential for aerosolization, splash, or
contamination and reduce risk of exposureEvaluate waste handlingIdentify how emergencies or spills could occur
& implement preventative measures
Testing of Biological Products
• Biological products (cell lines, blood, etc.) are the most common source for introduction of infectious disease to a colony
• ALL biological products must be tested prior to use (PCR, etc.)
• Even products (blood, serum) that have been passaged in rodents may be contaminated
Treatment/Prevention:Treatment/Prevention: oral & topical medications oral & topical medications and good sanitation, use PPE’sand good sanitation, use PPE’s
Ringworm Ringworm ((TrichophytonTrichophyton or or MicrosporumMicrosporum sp.) sp.)
Ringworm Ringworm ((TrichophytonTrichophyton or or MicrosporumMicrosporum sp.) sp.)
Treatment/Prevention:Treatment/Prevention: Oral medication, Oral medication, through sanitation/removal of wastes and through sanitation/removal of wastes and routine fecal analysis of colonyroutine fecal analysis of colony
Mode of Transmission: Mode of Transmission: exposure to exposure to saliva/urinesaliva/urine
Diagnosis:Diagnosis: ELISA & antibody test ELISA & antibody test
Treatment/Prevention:Treatment/Prevention: supportive care of supportive care of symptoms, sentinel program, us of PPE’ssymptoms, sentinel program, us of PPE’s
Note:Note: risk is primarily for wild-caught animalsrisk is primarily for wild-caught animals
Treatment/Prevention:Treatment/Prevention: anti-viral therapy, anti-viral therapy, supportive care of symptoms, good sanitation supportive care of symptoms, good sanitation and use of PPE’s and use of PPE’s
Note:Note: risk is primarily for wild-caught animalsrisk is primarily for wild-caught animals
Hantaviruses
Risk higher!!! Risk higher!!! RabiesRabies ListeriosisListeriosis LeptospirosisLeptospirosis TyphusTyphus Typhoid feverTyphoid fever Yersinia pestisYersinia pestis (plague) (plague) Other ecto- and endoparasitesOther ecto- and endoparasites
Scrub typhusScrub typhus Other rickettsial diseasesOther rickettsial diseases
Wild-caught Rodents
Summary
Complete all trainingFollow facility policiesUse PPEUnderstand risksFollow protocols and safety precautionsAsk questionsReport ANY incidents, problems, or injuriesContact us for help Questions???