9/29/21 1 I. Protein Structure A. Primary 1. Peptide Bond a. Planar, strong, f/y angles 2. Determination a. Sequence determination; CHEMICAL i. aa composition; Divide & conquer; Edman degradation b. Sequence determination; PHYSICAL i. Tandem Mass Spectrometry for proteins c. Sequence determination; BIOLOGICAL i. Genome sequenced; need partial sequence d. Determination of Disulfide bonds B. Secondary 1. Conformational structure; Levinthal paradox 2. Pauling & Corey’s predictions a. a-Helix b. b-sheets/strands c. Connections between b-strands d. Connections between a-helices; angle not important 3. Super secondary structure C. Tertiary 1. Picturing and classifications 2. Topology 3. Domains 4. Intrinsically disordered 5. Stability Lecture 9 (9/29/21) • Reading: Ch4; 114-115, 120- 121,123-124 • Problems: Ch4; 13 NEXT • Reading: Ch4; 116-117, 126-128 • Problems: Ch4; 7,15,16 Secondary Structure
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
9/29/21
1
I. Protein StructureA. Primary
1. Peptide Bonda. Planar, strong, f/y angles
2. Determination a. Sequence determination; CHEMICAL
i. aa composition; Divide & conquer; Edman degradationb. Sequence determination; PHYSICAL
i. Tandem Mass Spectrometry for proteinsc. Sequence determination; BIOLOGICAL
i. Genome sequenced; need partial sequenced. Determination of Disulfide bonds
B. Secondary1. Conformational structure; Levinthal paradox2. Pauling & Corey’s predictions
a. a-Helixb. b-sheets/strandsc. Connections between b-strandsd. Connections between a-helices; angle not important
3. Super secondary structure C. Tertiary
1. Picturing and classifications2. Topology3. Domains4. Intrinsically disordered5. Stability