POSTER PRESENTATIONS Molecular Biology and Biosynthesis Colchicine inhibits intraneuronal transport of neurosecretory material prior to axonal transport. G. ALONSO. Molecular forms of locust neuroparsins. D. BOUREME, A. GIRARDIE & J. GIRARDIE. POMC expression in foetal rat pituitary gland in organotypic culture. A. CARR, M.E. BAILEY & L.W. HAYNES. Proopiomelanocortin (POMC) gene expression in the developing rat spinal cord. A. CARR, S. BERRY & L.W. HAYNES. Melanin concentrating hormone: Structure activity studies of a new hormone. M.E. HADLEY, A.M.L. CASTRUCCI & V.J. HRUBY. Age-related changes in peptide and amine neurons in the rat hypothalamus. Y. IBATA, N. MORIMOTO, J. ABE, K. UDA, M. HASEGAWA, I. NAGATSU & N. YANAIHARA. A schistosome parasite inhibits female reproductive activity of its snail host by (inducing) production of schistosomin(s) which block the action of various female reproductive neuro- hormones. J. 100SSE, R. VAN ELK, R.H.M. EBBERINK, H.H. BOER, M. DE JONG-BRINK, W.J.A.G. DICTUS, M. ELSAADANY. In situ hybridization histochemistry for the analysis of gene expression in the oxytocin and vasopressin neuron system with radio labelled synthetic oligo-nucleotides. M. KAWATA, J.T. McCABE, D.W. PFAFF & Y. SANO. The presence of immunoreactive aMSH in the fish brain. M. KISHIDA & B.I. BAKER. Turnover of the vasopressin precursor in salt loaded rats. L.J. LAWSON & B.T. PICKERING. In search of the natriuretic hormone : Sodium transport inhibitors from hypothalamus. G.J. MOORE, P.J. COGGINS & P.M. KEANE. Processing of neurophysins in the rat neural lobe. R.W. NEWCOMB & J.J. NORDMANN. Environmental influences on the production and urophysial content of urotensins in the goby, Gillichthys mirabilis. D. PEARSON, Dj. ATMANI & D. PULLIAM. 251
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POSTER PRESENTATIONS
Molecular Biology and Biosynthesis
Colchicine inhibits intraneuronal transport of neurosecretory material prior to axonal transport. G. ALONSO.
Molecular forms of locust neuroparsins. D. BOUREME, A. GIRARDIE & J. GIRARDIE.
POMC expression in foetal rat pituitary gland in organotypic culture. A. CARR, M.E. BAILEY & L.W. HAYNES.
Proopiomelanocortin (POMC) gene expression in the developing rat spinal cord. A. CARR, S. BERRY & L.W. HAYNES.
Melanin concentrating hormone: Structure activity studies of a new hormone. M.E. HADLEY, A.M.L. CASTRUCCI & V.J. HRUBY.
Age-related changes in peptide and amine neurons in the rat hypothalamus. Y. IBATA, N. MORIMOTO, J. ABE, K. UDA, M. HASEGAWA, I. NAGATSU & N. YANAIHARA.
A schistosome parasite inhibits female reproductive activity of its snail host by (inducing) production of schistosomin(s) which block the action of various female reproductive neurohormones. J. 100SSE, R. VAN ELK, R.H.M. EBBERINK, H.H. BOER, M. DE JONG-BRINK, W.J.A.G. DICTUS, M. ELSAADANY.
In situ hybridization histochemistry for the analysis of gene expression in the oxytocin and vasopressin neuron system with radio labelled synthetic oligo-nucleotides. M. KAWATA, J.T. McCABE, D.W. PFAFF & Y. SANO.
The presence of immunoreactive aMSH in the fish brain. M. KISHIDA & B.I. BAKER.
Turnover of the vasopressin precursor in salt loaded rats. L.J. LAWSON & B.T. PICKERING.
In search of the natriuretic hormone : Sodium transport inhibitors from hypothalamus. G.J. MOORE, P.J. COGGINS & P.M. KEANE.
Processing of neurophysins in the rat neural lobe. R.W. NEWCOMB & J.J. NORDMANN.
Environmental influences on the production and urophysial content of urotensins in the goby, Gillichthys mirabilis. D. PEARSON, Dj. ATMANI & D. PULLIAM.
251
Co-existence of ir MCH and ir MSH in the carp neurohypophysis. K.A. POWELL & B.I. BAKER.
Insulin-like peptides are encoded on genes expressed in body growth controlling neuroendocrine cells of the mollusc lymnaea stagnalis. A.B. SMIT, R. DIRCKS & J. mOSSE.
Glucocorticoid induced changes in a-N-acetyl endorphin processing in the sheep anterior pituitary are centrally mediated. A.I. SMITH, C.A. WALLACE, I.J. CLARKE & J.W. FUNDER.
Does the pig testis contain two vasopressin-like peptides? A.J. SMITH, H.D. NICHOLSON & B.T. PICKERING.
Two related prohormone genes are expressed in egg-laying behaviour controlling neuroendocrine cells of the mollusc lymnaea stagnalis. E. VREUGDENHIL, W. VAN HEUMEN & W.P.M. GERAERTS.
Cell Biology of Neuropeptide Secretion
Suppression of neurohypophysial hormone release by opioids occurs without apparent change in pituicyte morphology. G. CLARKE & P.F. HEAP.
Role of connective tissue and glial cells in magnocellular neurosecretory axon regeneration. H.-D. DELLMANN, L.-F. LUE & S.I. BELLIN.
Changes in the three dimensional morphology of neurosecretory terminal arborisations during stimulation. R.E.J. DYBALL & F.D. SHAW.
Innervation of the hypophysial pars intermedia in the newt triturus cristatus and control of alpha-MSH release. A. FASOLO, M.F. FRANZONI, V. MAZZI, I. PERROTEAU, J.M. DANGER & H. VAUDRY.
Increased release of vasopressin and oxytocin from isolated rat neurohypophysis by gaba receptor stimulation. B. FJALLAND, J.D. CHRISTENSEN & S. GRELL.
Release of adipokinetic and hypertrehalosaemic neuropeptides from the corpora cardiaca of locusts, cockroaches and stick insects. G. GADE.
Ultrastructural localisation of a vasopressin immunoreactive precursor in the homozygous Brattleboro rat. S.E.F. GULDENAAR & B.T. PICKERING.
On separate roles of two types of gonadotrophin releasing hormone (GNRH) in the bird. S. ISHII, A. HATTORI, K. KUBOKAWA, S. MIKAMI, S. YAMADA & M. WADA.
Two functional and structural hemal compartments in the organum vasculosum laminae terminalis of the rat. B. KRISCH.
Receptors for atrial natriuretic peptide on pituicyte-like cells in culture. S.M. LUCKMAN & R.J. BICKNELL.
252
Effect of propranolol on immobilization stress-induced changes in oxytocin in the pituitary, hypothalamus and spinal cord. C. MIASKOWSKI, G.L. ONG & J. HALDAR.
Relaxin potentiates vasopressin release in rats in vivo. L.P. PARRY & A.J.S. SUMMERLEE.
The central delivery of neuropeptide hormones following neural transplantation in the endocrine hypothalamus of the brattleboro rat. D.E. SCOTT.
Hormone release from isolated neurohypophyseal nerve endings and swellings in...:rilm.. F.D. SHAW & J.F. MORRIS.
Release of antidiuretic hormone from specific axons in the corpus cardiacum of the house cricket, acheta domesticus, in response to water stress. J.H. SPRING.
Characterisation of an enriched fraction of nerve terminals (neurosecretosomes) from the bovine neurohypophysis. E.C. TOESCU, F.D. SHAW & J.F. MORRIS.
A morphological study of neurosecretory axons and endings in the rat neurohypophysis. C.D. TWEEDLE, K. SMITHSON & G.I. HATTON.
Effects of adrenalectomy on spinal cord vasopressin and oxytocin levels. J. VRBA, D. LUKIC & J. HALDAR.
Neurohypophysial actions of opioids involve K-opioid receptors localised on secretory terminals of magnocellular neurones. B-G. ZHAO, C. CHAPMAN & R.J. BICKNELL.
Morphological Techniques in the Study of Neuropeptides
Combined immunoperoxidase and radioimmunocytochemistry: a tool for double immunocytochemical labelling of peptidergic neurons. G. ALONSO.
Interconnections between vasopressinergic neurons in the suprachiasmatic nucleus. M. CASTEL, N. FEINSTEIN & S. COHEN.
Neurophysin-containing innervation of the mouse forebrain. M. CASTEL & J.F. MORRIS.
Immunocytochemical studies on the hypothalamo-hypophysial system of reptiles. P. FERNANDEZ-LLEBREZ, J. PEREZ, J.M. PEREZ-FIGARES & E.M. RODRIGUEZ.
Photoperiod induces changes in the immunostaining of the LHRH system of the Japanese quail. R.G. FOSTER, G.C. PANZICA, D.M. PARRY & C. VIGLIETTI-PANZICA.
Neuropeptides and serotonin in the nervous system of schistosoma mansoni. M.K.S. GUSTAFSSON.
Prolactin-like immunoreactivity in the brain and affiliated neuroendocrine structures of the insect leucophaea maderae. G.N. HANSEN, B.L. HANSEN & B. SCHARRER.
Oxytocin and the morphology of 9-day old rat testes. J. HUMPHRYS & R.T.S. WORLEY.
253
Cytochemical asymmetry of 5'-nucleotidase in the retinal muller (glial) plasma membrane: A possible role of edenosine as a neuromodulator. S.T. HUSSAIN & G.W. KREUTZBERG.
Development of neurosecretory nerve endings in the hypothalamus of bufo japonicus deprived of the pituitary primordium. K. KAWAMURA, S. KIKUYAMA & T. SHIBASAKI.
Development of preoptic recess organ in toad tadpoles. S. KIKUYAMA, K. KAWAMURA, M. MIYAKAWA, Y. ARAI & I. NAGATSU.
A system of liquor-contacting neurons, containing immunoreactive serotonin and somatostatin, in the hypothalamus of elasmobranchs. P. MEURLING, E.M. RODRIGUEZ & C.R. YULIS.
Fine structure of avian vasotocin-immunoreacting neurons. G.C. PANZICA, G.C. ANSELMETTI, G. CORVETTI & C. VIGLIETTI-PANZICA.
Substance P-Iike immunoreactivity demonstrated by the pap method in hydra attenuata. P. PIEROBON & M. KEMALI.
Morphometric analysis of the postnatal development of magnocellular neurones in the supraoptic nucleus of brattleboro and long evans rats. D.V. POW & I.F. MORRIS.
Differential distribution of acetylcholinesterase activity among immunocytochemically identified magnocellular neurosecretory neurones. D.V. POW & I.F. MORRIS.
Morphological and immunocytochemical evidence for exocytotic release of granules from multiple anatomically distinct compartments in the neural lobe of long evans and homozygous brattleboro rats. D.V. POW & I.F. MORRIS.
Histomorphological changes in the neurosecretory cells of an indian earthworm metaphire posthuma (vaillant) during development. O. PRASAD & PAWAN KUMAR.
Subsets of neurons in platyhelminths visualized by 5-HT, RF-amide and FMRF-amide antisera. M.REUTER.
Aminergic and peptidergic systems in the suprachiasmatic nucleus of an hibernator, Spermophilus richardsonii. C.M. SCHINDLER, A. KRIETE & F. NURNBERGER.
Peptidergic neurons in the Colorado potato beetle identified with polyclonal and monoclonal antibodies to regulatory peptides. H. SCHOONEVELD & I.A. VEENSTRA.
Detection of locust neuroparsin immuno-reactivity in invertebrates and vertebrates. M. TAMARELLE & I. GIRARDIE.
Urotensin-Iike immunoreactivity in the nervous system of flatworms - whole mount immunocytochemistry. M. WIKGREN.
Towards oxytocin function in the testes. R.T.S. WORLEY, I.R. CHALLIS, V.I. AYAD & M.A.C. APPLETON.
254
Electrical Activity of Peptidergic Neurones
Electrophysiological studies of cholecystokinin action on neurones in the ventromedial nucleus of rat hypothalamic slices. P. BODEN & R.G. HILL.
Neurohypophysial hormone release evoked by intraventricular hypertoxic sodium chloride: The significance of the subfornical organ. G. CLARKE & S.I. MacMILLAN.
Antagonists of 5-hydroxytryptamine reduce the effect of opioids on the milk-ejection reflex. G. CLARKE & D.M. WRIGHT.
The bed nucleus of the stria terminalis (BNST): A possible site for mediating the oxytocininduced facilitation of milk ejection. K.L. CUTLER, C.D. INGRAM & I.B. WAKERLEY.
Investigation of the faciHtatory effect of stimulation of the hypothalamo-neurohypophysial system on milk ejection. I. DAVISON, C.D. INGRAM & I.B. WAKERLEY.
Autoradiographic mapping of different types of neurohypophysial hormone-binding sites in the long evans rat forebrain and pituitary gland. M.I. FREUND-MERCIER, M.M. DIETL, M.E. STOECKEL, I.M. PALACIOS & PH. RICHARD.
Reduced oxytocin response to stress, hyperosmolality and electrical stimulation of the hypothalamo-neurohypophysial system in lactating rats. T. HIGUCHI, K. HONDA, S. TAKANO & H. NEGORO.
Activation of the paraventricular neurosecretory cells in osmotic stimulation of the anteroventral third ventricle in the rat. K. HONDA, H. NEGORO, T. HIGUCHI & S. T AKANO.
Patch clamp study of potassium channels in teleost fish rostral pars distalis cells. C.A. LORETZ & C.R. FOURTNER.
Functional interactions between oxytocin cells of the four magnocellular hypothalamic nuclei in anaesthetized suckled rats. F. MOOS, G. KUNTZELMAN & PH. RICHARD.
Specific release of oxytocin (OT) inside the hypothalamic magnocellular nuclei during the milk ejection reflex in rats. F. MOOS, D. POULAIN, Y. GUERNE, F. RODRIGUEZ, I.D. VINCENT & PH. RICHARD.
Frequency-dependent effects of different potassium channel blockers on the neurohypophysial release of vasopressin evoked by electrical stimulation. K. RACKE, D. JOST & H.P. HOBBACH.
Activation of centrally-projecting oxytocin neurones by naloxone in rats treated chronically with intracerebroventricular (i.c.v.) morphine. I.C.A.F. ROBINSON, J.E. COOMBES & J.A. RUSSELL.
Activity of putative vasopressinergic neurones in male and female rabbits. A.I.S. SUMMERLEE.
Relaxin effects the activity of oxytocin neurones in the conscious rat. A.I.S. SUMMERLEE, S.A. JONES & K.T. O'BYRNE.
255
Dynorphin-related pep tides act at kappa opiate receptors to inhibit secretion of oxytocin but not vasopressin from the magnocellular system during dehydration. J.Y. SUMMY-LONG, L.M. ROSELLA-DAMPMAN & G. McLEMORE.
Electrical and secretory activities of nerve terminals visualized by digital imaging. S. TERAKAWA & M. NAGANO.
Frequency facilitation of hormone release from the neural lobe may be mediated partly by stimulus-evoked increase in extracellular (K+). S.A. WAY. K. SHIBUKI & G. LENG.
256
PARTICIPANTS
G. ALONSO
B.I. BAKER
R.J. BICKNELL
G. BILBE
S.D. BIRKETT
P. BODEN
D. BOUREME
K. BROWN-GRANT
B.J. CANNY
M. CASTEL
V. CHAN-PALA Y
H.M. CHARLTON
J.D. CHRISTENSEN
G.CLARKE
I.J. CLARKE
Laboratoire de Neuroendocrinologie, Universite de Montpellier II, PI. E. Baraillon 3G060, Montpellier, Cedex, France.
School of Biological Sciences, Bath University, Claverton Down, Bath, BA2 7AY, UK.
A.F.R.C. Institute of Animal Physiology & Genetics Research, Babraham, Cambridge, CB2 4AT, UK.
2MBH, 1m Neuenheimer Feld 282, D-6900 Heidelberg I, West Germany.
Department of Anatomy, The Medical School, Bristol, BS8 lTD, UK.
Parke-Davis Research Unit, Addenbrookes Hospital Site, Cambridge, UK.
Universite de Bordeaux I, Laboratoire de Neuroendocrinologie, UA CNRA 1138, Avenue des Facultes, 33405 Talence Cedex, France.
Memorial University, Faculty of Medicine, St. John's, Newfoundland, Canada, AlB 3V6.
Medical Research Centre, Prince Henry's Hospital, St. Kilda Road, Melbourne, Australia 3004.
Department Experimental Zoology, Life Sciences, Hebrew University of Jerusalem, Israel.
Neurology Institute, University Hospital of Zurich, Zurich, CH-8091, Switzerland.
Department of Human Anatomy, South Parks Road, Oxford, OXI 3QX, UK.
Royal Danish School of Pharmacy, Department of Biology, Universitetsparken 2, 2100 Copenhagen 0, Denmark.
Department of Anatomy, The Medical School, Bristol, BS8 lTD, UK.
Medical Research Centre, Prince Henry's Hospital, St. Kilda Road, Melbourne, Australia 3004.
257
P.COBBETT
D. COLIN
B.A. CROSS
H.D. DELLMANN
P. DENNING-KENDALL
J.J. DREIFUSS
H.DUVE
R.E.J. DYBALL
R.G. DYER
A. FASOLO
A.F.R.C., Institute of Animal Physiology & Genetics Research, Babraham, Cambridge, CB2 4AT, UK.
Centre de Neurochimie, 5 rue Blaise Pascal, 67084 Strasbourg Cedex, France.
Institute of Animal Physiology & Genetics Research, Babraham, Cambridge, CB2 4AT, UK.
Department of Veterinary Anatomy, Iowa State University, Ames, IA 500 II, USA.
Department of Anatomy, The Medical School, Bristol, BS8 lTD, UK.
Department de Physiologie, Centre Medical Universitaire, 9 av de Champel, 1211 Geneve 4, Switzerland.
School of Biological Sciences, Queen Mary College, London University, Mile End Road, London, EI 4NS, UK.
University of Cambridge, Department of Anatomy, Downing Street, Cambridge, CB2 3DY, UK.
A.F.R.C., Institute of Animal Physiology & Genetics Research, Brabraham, Cambridge, CB2 4AT, UK.
Departimento Biologia Animale, Via Accademia Albertino, 17, 10123 Torino, Italy.
P. FERNANDEZ-LLEBREZ Department of Animal Physiology, Faculty of Sciences, Departamento de Fisiologia Animal, Facultad de Ciencias, 29071 Malaga, Spain.
B. FJALLAND
B.K. FOLLETT
G.GADE
H. GAINER
W.P.M. GERAERTS
C.L. GILBERT
A. GIRARDIE
J. GIRARDIE
258
Department of Biology, Royal Danish School of Pharmacy, Universitetsparken 2, 2100 Copenhagen 0, Denmark.
Department of Zoology, University of Bristol, Woodland Road, Bristol, BS8 lUG, UK.
Institut fUr Zoologie IV, der Universitlit DUsseldorf, Universitsstr. I, D-4000 DUsseldorf I, F.R.G.
National Institute of Child Health, NIH, Building 36, Room 2A21, Bethesda, Maryland 20205, USA.
Free University of Amsterdam, Biological Laboratory, de Boelelaan 1007, 1007 MC Amsterdam, The Netherlands.
Department of Anatomy, The Medical School, Bristol, BS8 lTD, UK.
Universite de Bordeaux I, Laboratoire de Neuroendocrinologie, Avenue des Facultes, 33405 Talence Cedex, France.
Universite de Bordeaux I, Laboratoire de Neuroendocrinologie, A venue des Facultes, 33405 Talence Cedex, France.
D.W. GOLDING
S.E.F. GULDENAAR
M.GUSTAFSSON
M.E. HADLEY
J. HALDAR
M.HAMAMURA
B.L. HANSEN
G.N. HANSEN
A.J. HARMAR
G.I. HATTON
L.W. HAYNES
P.M. HEADLEY
P.F. HEAP
T. HIGUCHI
R.G. HILL
S. HOLLINGSWORTH
K.HONDA
J. HUMPHRYS
S.T. HUSSAIN
Department of Zoology. The University. Newcastle upon Tyne. NEI 7RU. UK.
Department of Anatomy. The Medical School. Bristol. BS8 lTD. UK.
Department of Biology. Abo Akademi. Porthansgatan 3. SF-20500 Abo. Finland.
Department of Anatomy. University of Arizona. Tucson. AZ 85724. USA.
St. John's University. Department of Biological Sciences. Grand Central and Utopia Parkways. Jamaica. New York 11439. USA.
Department of Physiology. Jichi Medical School. Minamikawachi-Machi. Tochigi. Japan 329-04.
Institute of Medical Microbiology. University of Copenhagen, 22 Juliane Maries Vej, DK 2100 Copenhagen 0, Denmark.
Institute of Cell Biology and Anatomy. University of Copenhagen, 15 Universitetsparken, DK-2100 Copenhagen 0, Denmark.
Neuroscience Program, Michigan State University. East Lansing, MI 48824-1117, USA.
Department of Zoology, University of Bristol. Woodland Road, Bristol, BS8 lUG, UK.
Department of Physiology. The Medical School, Bristol, BS8 lTD, UK.
Department of Anatomy, The Medical School, Bristol, BS8 lTD, UK.
Fukui Medical School, Department of Physiology. Matsuoka, Fukui 910-11. Japan.
Parke-Davis Research Unit, Addenbrookes Hospital Site, Cambridge, UK.
A.F.R.C., Institute of Animal Physiology & Genetics Research, Babraham, Cambridge, CB2 4A T, UK.
Department of Physiology, Fukui Medical School, Matsuoka, Fukui, 910-11, Japan.
Department of Anatomy, The Medical School, Bristol, BS8 lTD, UK.
Department of Biological Sciences, Yarmouk University, Irbid, Jordan.
259
Y. IBATA
T. ICHIKAWA
C.D. INGRAM
S. ISHII
R.IVELL
J. JOOSSE
H. KANNAN
M. KASAl
K. KAWAMURA
M. KAWATA
P.M. KEEN
M. KEMALI
S. KIKUYAMA
M. KISHIDA
H. KOBAYASHI
B. KRISCH
K. KUBOKAWA
K. KUROSUMI
L.J. LAWSON
260
Department of Anatomy, Kyoto Prefectural University of Medicine, Kawaramachi Hirokoji, Kamikyoku Kyoto, Japan 602.
Tokyo Metropolitan Institute for Neurosciences, 2-6 Musashidai, Fuchu-City, Tokyo, Japan.
Department of Anatomy, The Medical School, Bristol, BS8 lTD, UK.
Department of Biology, Waseda University, Nishi-Waseda 1-6-1, Shiniuku-Ku, Tokyo 160, Japan.
Institute for Hormone and Fertility Research, Grandweg 64, 2000 Hamburg 54, West Germany.
Vrije U niversiteit, Biologisch Laboratorium, Postbus 7161, 1007 MC Amsterdam, The Netherlands.
Department of Physiology, University of Occupational and Environmental Health, Japan School of Medicine, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, 807 Japan.
Department of Physiology, University of Occupational and Environmental Health, Japan School of Medicine, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, 807 Japan.
Department of Biology, School of Education, Waseda University Nishiwaseda 1, Shinjuku-ku, Tokyo 160, Japan.
Department of Anatomy, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamikyo-ku, Kyoto 602, Japan.
Department of Pharmacology, The Medical School, Bristol, BS8 lTD, UK.
of bursting neurones in aplysia, 227-232 correlates of hormone release from sinus gland,
201-203 of cultured supraoptic neurones, 235-241 of GnRH neurones, 191-195 of neurosecretory terminals, 199-203 of oxytocin and vasopressin cells, 167-176, 181-
188, 219-223, 235-241 of sinus gland neurosecretory system, 207-216
Endocytosis, 144 Endocytotic vacuoles, 148 Endorp~,3O Enkephalins (see also Opioids), 93 Enkephalin-containing peptides, ~ Enkephalin