HURO/0901/1472.2.2 SZEGED - TIMISOARA AXIS FOR THE SAFE FOOD AND FEED SZETISA1 Disclaimer The content of this page does not necessarily represent the official position of the European Union. 3rd Progress Report Meeting Timisoara, 26-27 January, 2012 Macroelements in the Romanian wheat genotypes. Effects of Fusarium infection on macroelement concentrations in maize genotypes Erdei László, Vashegyi Ágnes, Pető Andrea Department of Plant Biology University of Szeged
19
Embed
HURO/0901/147 2.2.2 Szeged - Timisoara axis for the safe food and feed SZETISA1
HURO/0901/147 2.2.2 Szeged - Timisoara axis for the safe food and feed SZETISA1. Macroelements in the Romanian wheat genotypes . Effects of Fusarium infection on macroelement concentrations in maize genotypes. Erdei László, Vashegyi Ágnes, Pető Andrea Department of Plant Biology - PowerPoint PPT Presentation
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
HURO/0901/1472.2.2 SZEGED - TIMISOARA AXIS FOR THE SAFE FOOD AND FEED
SZETISA1
DisclaimerThe content of this page does not
necessarily represent the official position of the European Union.
K, Ca and Mg were determined in both parts by atomic absortion spectrophotometer (Hitachi Z-8200)
Manast
ur - TM
Sanan
drei -
TM
Fage
t-TM
Pietro
asa- T
M
Lovrin
-TM
Aldesti -T
M
Dudesti
i Vech
i -TM
Horea - A
r
Siria
- Ar
Recas -
Ar
Semlac
- Ar
Paulis
- Ar
Barzav
a - Ar
0.00
50.00
100.00
150.00
200.00
250.00 grain
chaff
μmol
K/g
DM
K concentrations (µmol K/g DM)
Manast
ur - TM
Sanan
drei -
TM
Faget-
TM
Pietro
asa- T
M
Lovrin
-TM
Aldesti -T
M
Dudest
ii Vech
i -TM
Horea -
Ar
Siria
- Ar
Recas -
Ar
Semlac
- Ar
Paulis
- Ar
Barzav
a - Ar
0.00
2.00
4.00
6.00
8.00
10.00
12.00
14.00
16.00 grain
chaffμm
ol C
a/g
DM
Ca concentrations (µmol Ca/g DM)
Manast
ur - TM
Sanan
drei - T
M
Faget-
TM
Pietro
asa- T
M
Lovrin
-TM
Aldesti -T
M
Dudesti
i Vech
i -TM
Horea -
Ar
Siria
- Ar
Recas -
Ar
Semlac
- Ar
Paulis
- Ar
Barzav
a - Ar
0.00
5.00
10.00
15.00
20.00
25.00
30.00
35.00 grain
chaffμm
ol M
g/ D
M
Mg concentrations (µmol Mg/g DM)
Conclusions
Potassium concentrations were similar in the grain, in Barzava and Faget slightly higher. In garain and chaff K levels near equal, but in some cases in grain it is higher or lower.
Calcium concentrations are 2 to 3-fold higher in chaff than in grains. In grains, no fluctuation was observed.
Magnesium concentrations are 2-fold higher in grains compared to tose in the chaff. The highest Mg concentrations in grains were fount at Barzava and Aldesti.
It is not known whether the differences are the consequences of the gentype or the agricultural site.
Macroelement concentrations in Fusarium-infected maize genotypes
From field experiments of Prof. Mesterházy Ákos, Cereal Research Nonprofit Ltd.
Maize genotypesZea mays L. cv. Sarolta – Fusarium resistantZea mays L. cv. Boglár – more sensitive
Fusarium inoculates
Fusarium graminearum (Fg), F. culmorum (Fc), F. verticillioides (Fv)
Infection: 8-10 days after flowering, with mycelia, using toothpick
Experimental design: 3 rows in 3 sites with repetitive rows of control and the 3 Fusarium strains. From each row 3 ears (with husks) and the leaves beneath were collected.
26.08.2
011.
13.09.2
011.
26.08
.2011
.
13.09.2
011.
0.00
50.00
100.00
150.00
200.00
250.00
300.00
C Fg
Fc Fv
µmol
K g
-1D
M-1
SAROLTA BOGLÁR
Effects of Fusarium infection on K concentration in maize kernels (from the middle part of the cob). Comparison of two sampling dates and 3 Fusarium species (Fg, Fc, Fv) in 2 maize genotypes. Mean +- S.D., n = 9.
26.08.2
011.
13.09.2
011.
26.08.2
011.
13.09.2
011.
0.00
0.50
1.00
1.50
2.00
2.50
C Fg
Fc Fv
umol
Ca
g-1
DM
-1
SAROLTA BOGLÁR
Effects of Fusarium infection on Ca concentration in maize kernels (from the middle part of the cob). Comparison of two sampling dates and 3 Fusarium species (Fg, Fc, Fv) in 2 maize genotypes. Mean+- S.D., n= 9.
26.08.2
011.
13.09
.2011
.
26.08.2
011.
13.09.2
011.
0.00
10.00
20.00
30.00
40.00
50.00
60.00
70.00
80.00 C
Fg
Fc
Fv
umol
Mg
g-1D
M-1
SAROLTA BOGLÁR
Effects of Fusarium infection on Mg concentration in maize kernels (from the middle part of the cob). Comparison of two sampling dates and 3 Fusarium species (Fg, Fc, Fv) in 2 maize genotypes. Mean+- S.D., n= 9.
Conclusions
Element concentrations (K, Ca, Mg) were higher in the infected kernels compared to the control.
Increase in element concentrations was proportional to the time passed after inoculation. Fist samling date was 8-10 days after inculation, the second one 3 weaks later, at harvest. By that time the infection was maximal and the kernels shrivelled.
Intensity of infection depended on the Fusarium species. Most agressive was F. graminearum, followed by F. culmorum and F. verticillioides.
Element concentration gradients along maize ears (base, middle, apex), in control and infected
samples
Control and Fusarium graminearum – infected maize genotype „Sarolta”
Base Middle Apex
Control - 36 kernels10 g
Infected -80 kernels10 g
Volume (and surface) relations of control and Fusarium – infected maize kernels
Base Middle Apex0
20
40
60
80
100
120
140
160SAROLTA C
FgFcFv
µmol
K g
-1D
M-1
Base Middle Apex0
50
100
150
200
250 BOGLÁR CFgFcFv
µmol
K g
-1D
M-1
Base Middle Apex0
20406080
100120140160180200 SAROLTA C
FgFcFv
µmol
K g
-1D
M-1
Base Middle Apex0
50
100
150
200
250
300 BOGLÁR CFgFcFv
µmol
K g
-1D
M-1
K concentration gradients along the maize ear as affected by Fusarium strains Upper row: samples at 26 August, lower row: samples at 13 September, 2011
Base Middle Apex0.00
0.20
0.40
0.60
0.80
1.00
1.20
1.40 SAROLTAC
Fg
Fc
Fv
µmol
Ca
g-1D
M-1
Base Middle Apex0.00
0.20
0.40
0.60
0.80
1.00
1.20 BOGLÁR CFgFcFv
µmol
Ca
g-1D
M-1
Base Middle Apex0.000.200.400.600.801.001.201.401.601.802.00
SAROLTAC
Fg
Fc
Fv
µmol
Ca
g-1D
M-1
Base Middle Apex0.0000.2000.4000.6000.8001.0001.2001.4001.6001.8002.000 BOGLÁR C
FgFcFv
µmol
Ca
g-1D
M-1
Ca concentration gradients along the maize ear as affected by Fusarium strains Upper row: samples at 26 August, lower row: samples at 13 September, 2011
Base Middle Apex0.05.0
10.015.020.025.030.035.040.045.0 SAROLTA C
FgFcFv
µmol
Mg
g-1D
M-1
Base Middle Apex0
10
20
30
40
50
60
70
80 BOGLÁR CFgFcFv
µmol
Mg
g-1D
M-1
Base Middle Apex0.0
10.0
20.0
30.0
40.0
50.0
60.0 SAROLTA CFgFcFv
µmol
Mg
g-1D
M-1
Base Middle Apex0.0
10.020.030.040.050.060.0
70.0BOGLÁR
CFgFcFv
µmol
Mg
g-1D
M-1
Mg concentration gradients along the maize ear as affected by Fusarium strains Upper row: samples at 26 August, lower row: samples at 13 September, 2011
Conclusions
In control, non-infected ears the element concentrations were near the same in the kernels taken from the basal, middle or apical part of the ear.In the dgnotype Sarolta, K concentration slightly increased, whereas Ca and Mg slightly decereased along the ear from base to apex.
At the infected middle partthe concentrations of all the elements incrreased. The enhancement was prportional with the agression of the Fusarium species, in the same order, as before.
Concentration values were calculated on unit mass basis. Since the infected kernels have much less mass than conntrol ones, per unit mass they containe larger number of kernels than the control sample. The higher element concentrations in the infected samples thus are du to the larger surface area of the kernels and/or the remaining tissues where these elements are lovalized.
Acknowledgements
Thanks are due toProf. Mesterházy Ákos and Dr. Toldiné dr. Tóth Éva for the field samples of wheat and maize and for their help.