Human Identification Applications on Ion Torrent PGM ™ Lisa Calandro Director, Product Management Human Identification, Life Technologies Special Acknowledgments Dr. Walther Parson, Institute of Legal Medicine, University of Innsbruck, Austria Dr. Kenneth Kidd, Department of Genetics, Yale University School of Medicine, USA
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Human Identification Applications on Ion Torrent PGM™
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Human Identification Applications on Ion Torrent PGM™
Lisa Calandro Director, Product Management Human Identification, Life Technologies Special Acknowledgments Dr. Walther Parson, Institute of Legal Medicine, University of Innsbruck, Austria Dr. Kenneth Kidd, Department of Genetics, Yale University School of Medicine, USA
• A “flow” is the event of exposing the chip to one particular dNTP (T, A, C, or G), followed by a washing step • A “cycle” is four consecutive dNTP flows: T-A-C-G = 1 cycle
Ion Sphere -----Primer------ A G T C A A G C G T C C C A T G
• A “flow” is the event of exposing the chip to one particular dNTP (T, A, C, or G), followed by a washing step • A “cycle” is four consecutive dNTP flows: T-A-C-G = 1 cycle
Ion Sphere -----Primer------ A G T C A A G C G T C C C A T G
• A “flow” is the event of exposing the chip to one particular dNTP (T, A, C, or G), followed by a washing step • A “cycle” is four consecutive dNTP flows: T-A-C-G = 1 cycle
Ion Sphere -----Primer------ A G T C A A G C G T C C C A T G
• A “flow” is the event of exposing the chip to one particular dNTP (T, A, C, or G), followed by a washing step • A “cycle” is four consecutive dNTP flows: T-A-C-G = 1 cycle
Ion Sphere -----Primer------ A G T C A A G C G T C C C A T G