Human Fibroblasts Replicate the in vivo environment with quality fibroblasts Research using fibroblasts plays an essenal role in the understanding of healing, human disease, and drug discovery. Fibroblasts synthesize the extracellular matrix and collagen, the structural framework (stroma) for ssues. Fibroblasts also play a crical role in wound healing. Research shows that dysfuncon of fibroblasts oſten leads to fibrosis. Fibrosis may in turn lead to other disorders such as anemia (1). Research using fibroblasts can also contribute to a beer understanding of genec diseases such as mucolipidosis II (ML-II) (2). These cells have also been used as a screening tool in the discovery of novel anbiocs in the fight against tuberculosis, an oſten lethal infecous disease (3). DV Biologics offers fibroblasts derived from normal donors as well as from donors with chronic or genec diseases (Table 1). These cells can be an invaluable source for your invesgaonal studies. Our product, skin fibroblasts (PI001-F), can be expanded over 40 populaon doublings (Fig. 1), without losing its morphological characteriscs. Immunocytochemical staining also shows that PI001-F cells synthesize collagen I/ III as they express fibroblasc markers (Fig. 2). Funconal assays of our fibroblasts demonstrate collagen secreon in the extracellular matrix (Fig. 3). As research moves away from animal models, it is oſten challenging for researchers to find cellular models that best represent the human physiological sengs. DV Biologics offers an expanding product porolio of unique cell types and ssue-derived products to help meet that challenge. Our team of dedicated sciensts is able to customize products to meet your specific research parameters. Our mission is to provide you with the biological tools needed for the innovaon of new technology that will one day be used to treat, or prevent, human degenerave disorders and diseases. All of our products are guaranteed and manufactured under ISO 9001:2008 guidelines. Figure 1. Growth Characteriscs of DV Biologics Skin Fibroblasts (PI001-F). A: Growth Curve: Cells were grown in DV Biologics I-GRO media, dissociated, counted, and subcultured (2×103 cells/ cm2) every 7-day period. The results indicate that DV Biologics Skin Fibroblasts (PI001-F) proliferate in culture for 8 or more passages, totaling over 40 populaon doublings. B: Phase Contrast Photomicrograph of passage 2 cells at 10X. PI001-F cells maintain the typical fibroblast morphology. Figure 2. Immunocytochemical Characterizaon of DV Biologics Skin Fibroblasts (PI001-F). Sub-confluent PI001-F culture was fixed, and labeled with an-fibroblast (green) and an-Collagen I/III (red) anbodies. Nuclei were labeled using DAPI (blue). A: 10× magnificaon; B: 40× magnificaon. The results indicate that DV PI001-F are posive for Collagen I/III, a funconal characterisc of fibroblasts.