EXPLORE THE DIABETES UNIVERSE WITH A SINGLE TECHNOLOGY HTRF ® assays for diabetes investigation
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3MD
050
- 08/
2015
- Co
pyri
ght C
isbi
o B
ioas
says
. All
righ
ts re
serv
ed. H
TRF
and
Tag-
lite
are
regi
ster
ed tr
adem
arks
of C
isbi
o B
ioas
says
.
A
CB
As the disease continues to impact the lives of an ever-growing number of people worldwide, Cisbio is simplifying the investigation of diabetes mechanisms with a portfolio of more than 20 assays—all built around a single technology and detection protocol—ideal for everything from target identification to screening to preclinical sample testing.
A WORLD EPIDEMIC. A GLOBAL SOLUTION.
ExPLORE ThE DIABETES UNIvERSE
WITh A SINGLE TEChNOLOGy
HTRF® assays for diabetes investigationFor more information or
to discuss your specific labeling or custom diabetes assay requirements, please
contact your local Cisbio representative or visit
www.cisbio.com
Cisbio bioassay Europe Sales and HeadquartersParc Marcel BoiteuxBP 8417530200 Codolet, FrancePhone: +33 (0)4 66 79 67 05Fax: +33 (0)4 66 79 19 [email protected]
Cisbio bioassay United States135 South RoadBedford, MA 01730, USAPhone: +1 888 963 4567Fax: +1 781 687 [email protected]
Cisbio bioassay Japan1-3 Nakase Mihama-ku Chiba-shi,Chiba Japan 261-8501Makuhari Techno Garden, 11F Building DPhone: +81-(0)43-306-8712Fax: +81-(0)[email protected]
Cisbio bioassay ChinaSuite 401, Building 21299 Zhangheng RoadZhangjiang Hi-Tech ParkPudong, Shanghai 201203, ChinaPhone: +86-21-5018-9880Fax: [email protected]
www.cisbio.com/metabolism-diabetes
3MD
050
- 08/
2015
- Co
pyri
ght C
isbi
o B
ioas
says
. All
righ
ts re
serv
ed. H
TRF
and
Tag-
lite
are
regi
ster
ed tr
adem
arks
of C
isbi
o B
ioas
says
.
A
CB
As the disease continues to impact the lives of an ever-growing number of people worldwide, Cisbio is simplifying the investigation of diabetes mechanisms with a portfolio of more than 20 assays—all built around a single technology and detection protocol—ideal for everything from target identification to screening to preclinical sample testing.
A WORLD EPIDEMIC. A GLOBAL SOLUTION.
ExPLORE ThE DIABETES UNIvERSE
WITh A SINGLE TEChNOLOGy
HTRF® assays for diabetes investigationFor more information or
to discuss your specific labeling or custom diabetes assay requirements, please
contact your local Cisbio representative or visit
www.cisbio.com
Cisbio bioassay Europe Sales and HeadquartersParc Marcel BoiteuxBP 8417530200 Codolet, FrancePhone: +33 (0)4 66 79 67 05Fax: +33 (0)4 66 79 19 [email protected]
Cisbio bioassay United States135 South RoadBedford, MA 01730, USAPhone: +1 888 963 4567Fax: +1 781 687 [email protected]
Cisbio bioassay Japan1-3 Nakase Mihama-ku Chiba-shi,Chiba Japan 261-8501Makuhari Techno Garden, 11F Building DPhone: +81-(0)43-306-8712Fax: +81-(0)[email protected]
Cisbio bioassay ChinaSuite 401, Building 21299 Zhangheng RoadZhangjiang Hi-Tech ParkPudong, Shanghai 201203, ChinaPhone: +86-21-5018-9880Fax: [email protected]
www.cisbio.com/metabolism-diabetes
[GLP
-1] p
g/m
L
700
600
500
400
300
200
100
00%
glucose2.5%
glucose10%
glucose5%
glucose
With our complete portfolio of diabetes assays, Cisbio gives you the tools you need
to research multiple targets and pathways using the same simple detection protocol.
Whether you are working with perfused pancreas, islets or isolated α or ß cells, our
HTRF assays have a proven track record of simplicity, performance and reliability.
INvESTIGATE DIABETES MORE QUICKLy, EASILy AND DEEPLy.
Adipocyte/inflammation Healthy adipocyte Pancreatic ß-cell/survival, proliferation
Pancreatic ß-cell/inflammation (Type 1 diabetes)
Pancreatic ß-cell/inflammation (Type 2 diabetes)
Hepatocyte Intestinal L-cell Muscle cell Pancreatic α-cell
HIGHLY VALIDATED ON RELEVANT DIABETES MODELS
No matter what your area of investigation, Cisbio assays can accelerate and simplify your day-to-day work, they also have a long history of performance and validation on your cell line for added confidence in your results.
Insu
lin o
utpu
t (ng
/min
)
40
30
20
10
0
35
25
15
5
25 45 8565
Time (min)
Methylsuccinate 2.5 µM+ ATP alpha S 2 µM
Methylsuccinate 2.5 µM
+ ATP alpha S 2 µM
[Glu
cago
n] p
g/m
L
700
600
500
400
50
25
0+High glucose
(10 mM)+Low glucose
(1 mM)+Adrenaline
(5 µM)
A B CGLP-1 secretion assay on human intestinal L-cells
Insulin assay on isolated perfused rat pancreas
Glucagon secretion assay on isolated mouse pancreatic islets
FEATURES AND BENEFITS
HTRF diabetes assays
• Homogeneous, mix-and-read, non-radioactive assays offer simpler, safer handling and enhanced throughput
• Proven HTRF technology features low sensitivity to interfering compounds and media for exceptional reliability
• Easy miniaturization delivers dramatic cost efficiencies
• Quick and easy development and implementation optimizes lab productivity
• Save time and money by consolidating your testing onto a single homogeneous platform
• Excellent reliability with minimal false positives
PRODUCT LIST: IL-1ß IL-6 TNFα GLP-1 Glucagon Insulin
[Ins
ulin
] ng/
mL
4000
3000
2000
1000
0-3.0 -2.5 -1.5-2.0
Log [Glucose] M
EC50 = 1.02 M S/B = 22
CYTOkINES AND HORMONES
Chronic inflammation and activation of the immune system have a proven connection to diabetes. The release of pro-inflammatory cytokines from adipocytes, including TNFα, IL-6 and IL-1ß correlates with the risk of insulin resistance in patients. Cisbio’s biomarker portfolio includes a number of markers directly associated with diabetes to further advance research in this area.
PRODUCT LIST: pAKT Total AKT Advanced pERK Total ERK pCREB pmTOR pS6RP pEIF4e pBAD p4EBP-1 pP70S6K pSTAT3 Total STAT3 pJNK pP38MAPK pIKKß pMEK1/2
kINASES AND pATHwAY READOUT
The ability to activate, inhibit or simply modulate major diabetes signaling nodes may lead to important drug discoveries. Pharmacological agents that selectively modulate kinase activities have the potential to reverse the metabolic abnormalities observed with type 2 diabetes. Cisbio’s ever-expanding portfolio of cell-based phospho-protein assays covers many of the pathways linked to diabetes.
PRODUCT LIST: Second Messengers cAMP IP-One Ligand Binding Tag-lite GLP-1 Tag-lite GIP
GpCRs
The importance of GPCRs in diabetes signal transduction and the resulting impact on glucose levels is widely recognized. Cisbio helps unravel the role of GPCRs in diabetes signaling with a range of tools stretching from the most established second messenger assays in the industry (IP-1 and cAMP) to our award-winning Tag-lite® technology for studying receptor ligand binding.
[cA
MP]
nM
500
400
300
200
100
0Basal [Glucose]
1 mM[Glucose]
10 mM[Glucose]
5 mM
Glucose-stimulated cAMP accumulation of pancreatic ß-cell MIN6
HTR
F Ra
tio
5000
3000
2000
1000
0
4000
-4.0 -3.0 -1.0-2.0
Log [Glucose] M-3.5 -2.5 -1.5
EC50 = 1.02 M S/B = 6
Glucose-stimulated ERK phosphorylation of pancreatic ß-cell MIN6
Glucose-stimulated insulin secretion of pancreatic ß-cell MIN6
Pancreatic ß-cell/ insulin secretion
INTERACTIVE ASSAY SELECTION TOOL
For help finding the ideal HTRF assay for your specific targets, please visit www.cisbio.com/metabolism-diabetes and click on the interactive Diabetes Navigator.
[GLP
-1] p
g/m
L
700
600
500
400
300
200
100
00%
glucose2.5%
glucose10%
glucose5%
glucose
With our complete portfolio of diabetes assays, Cisbio gives you the tools you need
to research multiple targets and pathways using the same simple detection protocol.
Whether you are working with perfused pancreas, islets or isolated α or ß cells, our
HTRF assays have a proven track record of simplicity, performance and reliability.
INvESTIGATE DIABETES MORE QUICKLy, EASILy AND DEEPLy.
Adipocyte/inflammation Healthy adipocyte Pancreatic ß-cell/survival, proliferation
Pancreatic ß-cell/inflammation (Type 1 diabetes)
Pancreatic ß-cell/inflammation (Type 2 diabetes)
Hepatocyte Intestinal L-cell Muscle cell Pancreatic α-cell
HIGHLY VALIDATED ON RELEVANT DIABETES MODELS
No matter what your area of investigation, Cisbio assays can accelerate and simplify your day-to-day work, they also have a long history of performance and validation on your cell line for added confidence in your results.
Insu
lin o
utpu
t (ng
/min
)
40
30
20
10
0
35
25
15
5
25 45 8565
Time (min)
Methylsuccinate 2.5 µM+ ATP alpha S 2 µM
Methylsuccinate 2.5 µM
+ ATP alpha S 2 µM
[Glu
cago
n] p
g/m
L
700
600
500
400
50
25
0+High glucose
(10 mM)+Low glucose
(1 mM)+Adrenaline
(5 µM)
A B CGLP-1 secretion assay on human intestinal L-cells
Insulin assay on isolated perfused rat pancreas
Glucagon secretion assay on isolated mouse pancreatic islets
FEATURES AND BENEFITS
HTRF diabetes assays
• Homogeneous, mix-and-read, non-radioactive assays offer simpler, safer handling and enhanced throughput
• Proven HTRF technology features low sensitivity to interfering compounds and media for exceptional reliability
• Easy miniaturization delivers dramatic cost efficiencies
• Quick and easy development and implementation optimizes lab productivity
• Save time and money by consolidating your testing onto a single homogeneous platform
• Excellent reliability with minimal false positives
PRODUCT LIST: IL-1ß IL-6 TNFα GLP-1 Glucagon Insulin
[Ins
ulin
] ng/
mL
4000
3000
2000
1000
0-3.0 -2.5 -1.5-2.0
Log [Glucose] M
EC50 = 1.02 M S/B = 22
CYTOkINES AND HORMONES
Chronic inflammation and activation of the immune system have a proven connection to diabetes. The release of pro-inflammatory cytokines from adipocytes, including TNFα, IL-6 and IL-1ß correlates with the risk of insulin resistance in patients. Cisbio’s biomarker portfolio includes a number of markers directly associated with diabetes to further advance research in this area.
PRODUCT LIST: pAKT Total AKT Advanced pERK Total ERK pCREB pmTOR pS6RP pEIF4e pBAD p4EBP-1 pP70S6K pSTAT3 Total STAT3 pJNK pP38MAPK pIKKß pMEK1/2
kINASES AND pATHwAY READOUT
The ability to activate, inhibit or simply modulate major diabetes signaling nodes may lead to important drug discoveries. Pharmacological agents that selectively modulate kinase activities have the potential to reverse the metabolic abnormalities observed with type 2 diabetes. Cisbio’s ever-expanding portfolio of cell-based phospho-protein assays covers many of the pathways linked to diabetes.
PRODUCT LIST: Second Messengers cAMP IP-One Ligand Binding Tag-lite GLP-1 Tag-lite GIP
GpCRs
The importance of GPCRs in diabetes signal transduction and the resulting impact on glucose levels is widely recognized. Cisbio helps unravel the role of GPCRs in diabetes signaling with a range of tools stretching from the most established second messenger assays in the industry (IP-1 and cAMP) to our award-winning Tag-lite® technology for studying receptor ligand binding.
[cA
MP]
nM
500
400
300
200
100
0Basal [Glucose]
1 mM[Glucose]
10 mM[Glucose]
5 mM
Glucose-stimulated cAMP accumulation of pancreatic ß-cell MIN6
HTR
F Ra
tio
5000
3000
2000
1000
0
4000
-4.0 -3.0 -1.0-2.0
Log [Glucose] M-3.5 -2.5 -1.5
EC50 = 1.02 M S/B = 6
Glucose-stimulated ERK phosphorylation of pancreatic ß-cell MIN6
Glucose-stimulated insulin secretion of pancreatic ß-cell MIN6
Pancreatic ß-cell/ insulin secretion
INTERACTIVE ASSAY SELECTION TOOL
For help finding the ideal HTRF assay for your specific targets, please visit www.cisbio.com/metabolism-diabetes and click on the interactive Diabetes Navigator.
3MD
050
- 08/
2015
- Co
pyri
ght C
isbi
o B
ioas
says
. All
righ
ts re
serv
ed. H
TRF
and
Tag-
lite
are
regi
ster
ed tr
adem
arks
of C
isbi
o B
ioas
says
.
A
CB
As the disease continues to impact the lives of an ever-growing number of people worldwide, Cisbio is simplifying the investigation of diabetes mechanisms with a portfolio of more than 20 assays—all built around a single technology and detection protocol—ideal for everything from target identification to screening to preclinical sample testing.
A WORLD EPIDEMIC. A GLOBAL SOLUTION.
ExPLORE ThE DIABETES UNIvERSE
WITh A SINGLE TEChNOLOGy
HTRF® assays for diabetes investigationFor more information or
to discuss your specific labeling or custom diabetes assay requirements, please
contact your local Cisbio representative or visit
www.cisbio.com
Cisbio bioassay Europe Sales and HeadquartersParc Marcel BoiteuxBP 8417530200 Codolet, FrancePhone: +33 (0)4 66 79 67 05Fax: +33 (0)4 66 79 19 [email protected]
Cisbio bioassay United States135 South RoadBedford, MA 01730, USAPhone: +1 888 963 4567Fax: +1 781 687 [email protected]
Cisbio bioassay Japan1-3 Nakase Mihama-ku Chiba-shi,Chiba Japan 261-8501Makuhari Techno Garden, 11F Building DPhone: +81-(0)43-306-8712Fax: +81-(0)[email protected]
Cisbio bioassay ChinaSuite 401, Building 21299 Zhangheng RoadZhangjiang Hi-Tech ParkPudong, Shanghai 201203, ChinaPhone: +86-21-5018-9880Fax: [email protected]
www.cisbio.com/metabolism-diabetes
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