Analysis of Nitrosodiethylamin and Nitrosodiprophylamin in Sausage using Single Drop Microextraction Coupled with GC Zarah Nur Intissar (080710150) Counsellor : Reviewer : 1.Dra. Aning Purwaningsih, M.Si. Dra. Usreg Sri Handajani, M.Si 2.Yanuardi Raharjo, S.Si., M.Sc.
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Analysis of Nitrosodiethylamin and Nitrosodiprophylamin in Sausage using Single Drop
Microextraction Coupled with GC
Zarah Nur Intissar
(080710150)
Counsellor : Reviewer :
1.Dra. Aning Purwaningsih, M.Si. Dra. Usreg Sri Handajani, M.Si
2.Yanuardi Raharjo, S.Si., M.Sc.
Introduction
CHAPTER I
BACKGROUND
Food Preservative
Nitrite Carcinogen
NitrosamineNitrosodiethylamine
(NDEA)Nitrosodiprophylamine
(NDPA)
Sausage
NitrosamineLow Concentration
( ppb )Sample preparation
(extraction)
Single Drop Microextraction
(SDME)
Direct-SDME
Headspace-SDME
BACKGROUND
PROBLEMS OF RESEARCH
o Are the Nitrosodiethylamine and Nitrosodiprophylamin can be analyzed using HS-SDME?
o How the optimation result of speed of squealer, volume of organic phase and time of extraction for HS-SDME applicated to the analysis of NDEA and NDPA?
o Are the HS-SDME can be used for analysis of NDEA and NDPA in sausage?
OBJECTIVE OF RESEARCH
o To analysis of NDEA and NDPA using HS-SDME.
o To determine the optimum for speed of squealer, volume of organic phase and time of extraction for anaysis NDEA and NDPA using HS-SDME.
o To know that the result of optimum parameters can be used for analysis of NDEA and NDPA in sausage.
BENEFIT OF RESEARCH
To produce a new method to the analysis NDEA and NDPA quickly, selective and cheap in sausage.
To know the concentration of nitrosamine in sausage so that we can anticipation for the negative effect from nitrosamine.
BAB II
Tinjauan Pustaka
• Karsinogenik• Nitrit yang bereaksi pada
kondisi sangat asam• Salah satu senyawa
turunan nitrosamin yakni NDEA dan NDPA
Nitrosamin
• Makanan favorit• Pembuatan sosis• Macam sosis yakni sosis
mentah dan sosis siap makan
Sosis
Single-Drop Microextraction
(SDME)
Direct-SDME
Headspace-SDME(HS-SDME)
Drop pelarut organik di rendam langsung di dalam
larutan sampel
Drop pelarut organik di letakkan di atas
larutan sampel (menggantung)
Gas Chromatography (GC)
Jenis kromatografi untuk memisahkan dan menganalisa campuran zat
Komponen instrumen kromatografi gas
BAB III
Metode Penelitian
ALAT DAN BAHAN PENELITIAN
Alat Penelitian :
•Gelas beaker 25 ml
•Botol kaca 15 ml
•Botol kaca 2 ml
•Botol kaca 100 ml
•Mortar
•GC
•Microsyringe 25 µl
•Mikropipet 1000 µl
•Tube mikropipet
•Labu ukur 10 ml
•Shaker
•Saringan
Bahan Penelitian
•Metanol 70 %
•NDEA 99,99 %
•NDPA 99,99 %
•Sosis
•n-heksan
SKEMA KERJA
Syringe berisi pelarut organik
Jarum syringe
Larutan pengekstraksi (drop pelarut organik)
Pengaduk magnetik
Larutan Standar/sampel
Proses Ekstraksi HS-SDME
Setelah proses ekstraksi selesai, pelarut organik ditarik ke dalam syringe kemudian diinjeksikan ke GC