HPLC • GC • SPE - Separationsseparations.co.za/fileadmin/themes/default/pdf/2... · HPLC SPE Strata ® Accessories BioSeparations PREP / Process GC 2 Strata Impact Precipitation

2009 Key Products Buyer’s GuideHPLC • GC • SPE

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TrademarksClarity, Gemini, Jupiter, Luna and Strata are registered trademarks of Phenomenex, Inc. Axia, BioSep, Cool-Lock, EZ:faast, FilterSys, Giga, Impact, Inferno, MercuryMS, MultiResidue, Oligo-RP, Oligo-WAX, Onyx, pH-LC, Phenex, PREPLC, QSP, Rezex, SecurityGuard, Strata-X, Synergi, Triophilic, TWIN, TWIN-NX, WAXPLUS, Z-Guard and Zebron are trademarks of Phenomenex, Inc. Alltima is a trademark of Alltech Associates, Inc. Bio-Sil and Aminex are registered trademarks of Bio-Rad Laboratories. CHIRALCEL and OD-H are registered trademarks of DAICEL Chemical Industries, Ltd. Cyrolite is a trademark of CY/Ro Industries. HYPERSIL is a registered trademark of Thermo Hypersil-Keystone. OBD is a trademark of Waters Corp. Pursuit is a registered trademark of Varian, Inc. Sugar-Pak is a trademark of Waters Corporation. Santoprene is a trademark of Exxon Mobil Corporation. Supelcogel is a trademark of Supelco, Inc. Symmetry and XTerra are registered trademarks of Waters Corp. J&W is a registered trademark of Agilent. DB-5ht is a trademark of Agilent Technologies. SOURCE is a trademark of GE Healthcare Companies. Stabilwax is a registered trademark of Restek. GE is a trademark of General Electric Company. Teflon is a registered trademark of E.I. du Pont de Nemours and Co. ZORBAX is a registered trademark of Agilent Technologies. Phenomenex Inc. is not affiliated with the above companies.

DisclaimersGemini-NX is patent pending. Onyx is a product based on monolithic technology under license from Merck KGaA, Darmstadt, Germany. The comparative data presented may not be representative for all applications. Subject to Phenomenex Standard Terms & Conditions which may be viewed at www.phenomenex.com/TermsAndConditions.

© 2008 Phenomenex, Inc. All rights reserved.

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GC

SPE

ACCESSORIES

HPLC

PREP/PROCESS

BIOSEPARAtIOnS

AMInO ACID AnALYSIS

FOOD AnD BEVERAGE AnALYSIS


HP

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Strata Impact Precipitation PlatesPart No. Description Unit/Box PriceStrata Impact Precipitation PlatesCE0-7565 Strata Impact Protein Precipitation, Square Well, Filter Plate, 2 mL 2 Strata Impact Starter Kit for Protein PrecipitationCE0-8201 Strata Impact Protein Precipitation Plate (2 ea) Collection Plate 2 mL

(2 ea) Sealing Mat, Santoprene™ (AH0-8199) (2 ea)ea

Strata® Impact Protein Precipitation Plates • Direct in-plate precipitation for faster analysis • Compatibility with organic solvents for clean, leak-free analysis • Efficient precipitation with no cross-contamination or clogging

Protein Precipitation using Acetonitrile (4:1 Solvent Sample Ratio)

App ID 15587 Precipitation Conditions

Format: Strata Impact Protein Precipitation Plate, 2 mL Part No.: CE0-7565 Dispense: 400 μL Acetonitrile into empty wells Add: 200 μL sample to each well Mix: Sample and organic for 30 seconds Apply: Vacuum to sample, collect and inject filtrate

Analytical Column

HPLC Column: Onyx® Monolithic C18 Dimensions: 100 x 4.6 mm Part No.: CH0-7643 Mobile Phase: A: 0.1 % Formic acid in Water B: 0.1 % Formic acid in Acetonitrile Gradient: 10-65 % B in 4 min Flow Rate: 4.0 mL/min Temperature: Ambient Detection: LC/MS ESI+ Peaks: 1. Pseudoephedrine (m/z = 166) 2. Propranolol (m/z = 260) 3. Diltiazem (m/z = 415) 4. Verapamil (m/z = 455)

Protein Precipitation using Acetonitrile (3:1 Solvent Sample Ratio)

App ID 15712 Precipitation Conditions

Format: Strata Impact Protein Precipitation Plate, 2 mL Part No.: CE0-7565 Dispense: 300 μL Acetonitrile into empty wells Add: 100 μL of porcine plasma to each well Mix: For 30 sec or let sample stand for 2 min Apply: Vacuum to sample and collect the filtrate and blow down to dryness under slow stream of nitrogen @ 40 ºC Reconstitute: With 100 μL of mobile phase containing 10 ng of analyte of interest

Analytical Column

HPLC Column: Gemini® 3 μm C18 110 Å Dimensions: 150 x 3.0 mm Part No.: OOF-4439-Y0 Mobile Phase: A: 0.1 % Formic acid in Water B: 0.08 % Formic acid / Acetonitrile Gradient: A/B = 90/10 to 50/50 in 5 min, hold for 2 min Flow Rate: 0.5 mL/min Temperature: Ambient Detection: LC/MS ESI+ Injection: 10 μL of sample Sample: 1. Pindolol 2. Metoprolol

0 1 2 3 min

1

2

34

15712

Blank Pindolol

Sample Pindolol

Blank Metoprolol

Sample Pindolol

0 1 2 3 4 50 1 2 3 4 5 6 min

1

2

SIMPLE & CLEAn PRECIPItAtIOn

No More Spin Down

No More Transfer Steps

No More Long Waiting

Acrylic ChamberManifold Lid (Top)

Strata ImpactPrecipitation Plate

Manifold Base (Bottom)

Collection Plate(Hidden Inside)

*Square well sealing mats compatible with 2 mL Strata Impact plates, Strata and Strata-X 96-well plates, and 96 square well collection plates.

Sealing Mats*Part No. Description Unit PriceAH0-7195 Sealing Mats, Pierceable, 96 Square Well, Ethylene Vinyl Acetate (EVA) 50/pk AH0-8199 Sealing Mats, Pierceable, 96 Square Well, Santoprene™ 100/pk

Collection Plates* and 96-Well Plate Vacuum ManifoldPart No. Description Unit PriceAH0-7284 96-well plate manifold, acrylic eaAH0-7192 350 µL/well 96 Square Well Conical V-bottom Collection Plate 50/pk AH0-7193 1 mL/well 96 Square Well Conical V-bottom Collection Plate 50/pkAH0-7194 2 mL/well 96 Square Well Conical V-bottom Collection Plate 50/pk AH0-7279 1 mL/well 96 Round Well Round Bottom Collection Plate 50/pk

ORDERING INFORMATION


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Sample Preparation – SPEStrata™-X* Polymeric Sorbents • Excellent tube-to-tube and well-to-well reproducibility • Simple and fast method development • High recoveries for acidic, basic, polar, and non-polar analytes

Sorbent Functional Group Mode Analyte

Strata-X

Strata-XLReversed Phase Polar

& Non-Polar

Strata-X-C

Strata-XL-C

Reversed Phase & Strong Cation Exchange

Bases

Strata-X-CWReversed Phase & Weak Cation Exchange

Bases (including Quaternary Amines)

Strata-X-AWReversed Phase & Weak Anion Exchange

Acids

N ..

n )

)

n )

)

n )

)

n )

)

O

OH

H+

O- S

O

O

NH NH2

O

N ..

n )

)

n )

)

n )

)

n )

)

O

OH

H+

O- S

O

O

NH NH2

O N ..

n )

)

n )

)

n )

)

n )

)

O

OH

H+

O- S

O

O

NH NH2

O N ..

n )

)

n )

)

n )

)

n )

)

O

OH

H+

O- S

O

O

NH NH2

O


Traditional Tubes 1 mL (100/box) 3 mL (50/box) 6 mL (30/box)

Phase 30 mg 60 mg 200 mg 500 mg 100 mg 200 mg 500 mg

Strata-X 8B-S100-TAK 8B-S100-UBJ 8B-S100-FBJ 8B-S100-HBJ 8B-S100-ECH 8B-S100-FCH 8B-S100-HCH

Strata-X-C 8B-S029-TAK 8B-S029-UBJ 8B-S029-FBJ 8B-S029-HBJ 8B-S029-ECH 8B-S029-FCH 8B-S029-HCH

Strata-X-CW* 8B-S035-TAK 8B-S035-UBJ 8B-S035-FBJ 8B-S035-HBJ 8B-S035-ECH 8B-S035-FCH 8B-S035-HCH

Strata-X-AW 8B-S038-TAK 8B-S038-UBJ 8B-S038-FBJ 8B-S038-HBJ 8B-S038-ECH 8B-S038-FCH 8B-S038-HCH

*Strata-X-CW in 1 g/6 mL is available. Please call Phenomenex for more information.

Strata-X Giga™ Tubes 12 mL (20/box) 20 mL (20/box) 1

Phase 500 mg 1 g 1 g

Strata-X 8B-S100-HDG 8B-S100-JDG 8B-S100-JEG

Strata-X-C 8B-S029-HDG 8B-S029-JDG ––

Strata-X-CW 8B-S035-HDG 8B-S035-JDG 8B-S035-JEG

Strata-X-AW 8B-S038-HDG 8B-S038-JDG ––

High-Throughput 96-Well Plates (2/box)

Phase 5 mg 10 mg 30 mg 60 mg

Strata-X 8E-S100-NGB 8E-S100-AGB 8E-S100-TGB 8E-S100-UGB

Strata-X-C –– 8E-S029-AGB 8E-S029-TGB 8E-S029-UGB

Strata-X-CW –– 8E-S035-AGB 8E-S035-TGB 8E-S035-UGB

Strata-X-AW –– 8E-S038-AGB 8E-S038-TGB 8E-S038-UGB

POLYMERIC SORBEntS FOR SIMPLIFIED SPE

Strata-X Giga™ Tubes12 mL (20/box) 20 mL (20/box) 60 mL (16/box)

Phase 2 g 2 g 5 g 5 g 10 g

Strata-XL 8B-S043-KDG 8B-S043-KEG 8B-S043-LEG 8B-S043-LFF 8B-S043-MFF

Strata-XL-C 8B-S044-KDG 8B-S044-KEG 8B-S044-LEG 8B-S044-LFF 8B-S044-MFF

*Strata-X is patented by Phenomenex, Inc.


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top Solutions for Environmental Laboratories

Zebron™ ZB-5MSi • Temperature limits: -60 to 360/370 ˚C (Isothermal/TPGC) • Highly inert - improved peak shape for acidic/basic compounds • MS certified low-bleed levels provide maximum sensitivity • Great results for pesticides, phenols, nitrosamines, and EPA Methods

QUALItY HIGH PERFORMAnCE GC COLUMnS

Alternative to Any 5 %-Phenyl- 95 %-Dimethylpolysiloxane Phase:DB-5 Rtx-5ms MDN-5S HP-5ms Rtx-5Amine HP-5msi Rxi-5ms

Certified

MSeSCequipped

6 8 10 12 14 16 18 20 22 min

1 23

4,5

67

812

9,10,11

13,14,15

161718 19 20

21

2223

24

25

2627

If you need a 5 in. cage, simply add a (-B) after the part number, e.g., 7HG-G018-11-B.

ProtECt your GC CoLumn. try Z-Guard witH your nExt ZEbron ordEr.

App

ID 1

6410 Phenols

1. Phenol 2. 2-Chlorophenol 3. 2-Methylphenol 4. 4-Methylphenol 5. 3-Methylphenol 6. 2,6-Dimethylphenol 7. 2-Nitrophenol 8. 2-Ethylphenol 9. 2,4-Dimethylphenol 10. 3,5-Dimethylphenol 11. 2,5-Dimethylphenol 12. 4-Ethylphenol 13. 3-Ethylphenol 14. 2,4-Dichlorophenol

15. Benzoic Acid 16. 2,3-Dimethylphenol 17. 3,4-Dimethylphenol 18. 2.6-Dichlorophenol 19. 4-Chloro-3-methylphenol 20. 2,4,6-Trichlorophenol 21. 2,4,5-Trichlorophenol 22. 2,4-Dinitrophenol 23. 4-Nitrophenol 24. 2,3,4,6-Tetrachlorophenol 25. 4,6-Dinitro-2-methylphenol 26. Pentachlorophenol 27. Dinoseb

Column: Zebron ZB-5MSi Dimensions: 30 meter x 0.25 mm x 0.25 μm Part No.: 7HG-G018-11 Injection: Split 5:1@ 240 ˚C, 1 μL Carrier Gas: Helium @ 1.2 mL/min (constant flow) Oven Program: 60 ˚C to 140 ˚C @ 5 ˚C/min to 280 ˚C @ 10 ˚C/min Detector: MSD @ 230 ˚C; 45-450 amu Sample:

ID(mm) df(µm) Temp. Limits °C Part No. Price15-Meter I

I I I 0.25 0.25 -60 to 360/370 7EG-G018-11 30-Meter0.25 0.25 -60 to 360/370 7HG-G018-110.25 0.50 -60 to 360/370 7HG-G018-17 0.32 0.25 -60 to 360/370 7HM-G018-11 0.32 0.50 -60 to 360/370 7HM-G018-17 60-Meter0.25 0.25 -60 to 360/370 7KG-G018-11 Test MixZebron ZB-5MSi AGO-8362



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Zebron™ ZB-5Ht Inferno™ • First non-metal column stable to 430 ˚C • Bake off contaminates at extremely high temperatures • Longer column lifetime for high boiling compounds, long-chained hydrocarbons, and surfactants


16144

6 8 10 12 14 16 18 20 min

1

2

34

6

78

910

111213

14

1516

1718

19

5Zebron Inferno at

350 ºCGreat detection of late eluting peak!

2007 R&D 100 Award Recipient

If you need a 5 in. cage, simply add a (-B) after the part number, e.g., 7HG-G015-11-B.*0.53 mm ID columns are rated to 400 ˚C max operational



I I I 0.25 0.10 -60 to 400/430 7EG-G015-02 0.32 0.10 -60 to 400/430 7EM-G015-02 0.32 0.25 -60 to 400/430 7EM-G015-11 0.53 0.15 -60 to 400 7EK-G015-05 20-Meter0.18 0.18 -60 to 400/430 7FD-G015-08 30-Meter0.25 0.10 -60 to 400/430 7HG-G015-02 0.25 0.25 -60 to 400/430 7HG-G015-11 0.32 0.10 -60 to 400/430 7HM-G015-02 0.32 0.25 -60 to 400/430 7HM-G015-11 0.53 0.15 -60 to 400 7HK-G015-05 Test MixZebron ZB-5HT AG0-515530-Meter 5 Meter Guardian0.25 0.10 -60 to 400/430 7HG-G015-02-GGA

Alternative to Any 5 %-Phenyl-95 %-Dimethylpolysiloxane High-temperature Phase:DB-5ht VF-5ht HT-5 Stx-5HT XTI-5HT

PolyBrominated Diphenyl Ethers (PBDEs)

Column: Zebron ZB-5HT Inferno

Dimensions: 15 meter x 0.25 mm x 0.10 μm

Part No.: 7EG-G015-02

Injection: On-Column @ 73 ºC, 0.5 μL

Carrier Gas: Helium @ 1.5 mL/min (constant flow)

Oven Program: 70 ºC to 160 ºC @ 25 ºC/min to 350 ºC @ 10 ºC/min for 5 min

Detector: ECD @ 400 ºC

Sample: 1. BDE-25 11. BDE-992. BDE-28 12. BDE-853. BDE-75 13. BDE-1544. BDE-49 14. BDE-1535. BDE-71 15. BDE-1386. BDE-47 16. BDE-1837. BDE-66 17. BDE-1908. BDE-77 18. BDE-2039. BDE-100 19. BDE-209

10. BDE-119

App

ID 1

6144

Note: Sample was 2.5 ppm in iso-octane.

Certified

MSeSCequipped

Outperforms Agilent J&W DB-5htLifetime Comparison†

7.0

7.5

8.0

200 40 60 80

Hours at 400 °C

Ret

entio

n Ti

me

(min

)

Zebron™ ZB-5HT Inferno™

Agilent® J&W® DB-5ht™

Conditions for all columns:


Injection: 1.0 μL of test mix AG0-7578

Carrier Gas: Constant Flow Helium @ 1.9 mL/min

Oven Program: 120 °C Isothermal

Detector: Flame Ionization Detector (FID) @ 400 °C

Sample: Pentadecane

Longer Lifetime!

Zebron ZB-5HT outperforms Agilent J&W DB-5ht. Columns were held at 400 °C for 2 hours, then the oven was lowered to 120 °C for Pentadecane analysis.† All columns were new, never used, prior to this testing and purchased either directly from the original manufacturer or through an authorized distributor. All testing was carefully controlled to ensure conditions were similar for all columns involved. The comparative data is not representative of every application.



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Similar to:MDN-12 DB-XLB Rtx-XLB

top Solutions for Environmental Laboratories

Zebron™ ZB-XLB • Low polarity Si-arylene column • Provides alternate selectivity to standard 5-type phases • Low bleed and sensitivity levels designed for MS detectors • Great for confirmation of pesticides, PCBs, or other environmental samples • Good tool for identifying unknown contaminants

If you need a 5 in. cage, simply add a (-B) after the part number, e.g., 7HG-G019-11-B.*Thicker films(≥1.0 um df) are rated to 320/340 ˚C (Isothermal/TPGC).



I I I 0.25 0.25 30 to 340/360 7EG-G019-11 20-Meter0.18 0.18 30 to 340/360 7FD-G019-08 30-Meter0.25 0.25 30 to 340/360 7HG-G019-11 0.25 0.50 30 to 340/360 7HG-G019-17 0.32 0.25 30 to 340/360 7HM-G019-11 0.32 0.50 30 to 340/360 7HM-G019-17 0.53 1.50 30 to 320/340 7HK-G019-28 60-Meter0.25 0.25 30 to 340/360 7KG-G019-11 Test MixZebron ZB-XLB AG0-7578

8 9 10 11 12 13 14 15 16 min

1 2

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4

56 7

89

10

11

12

13

1415

1617 18

19

20

Certified

MSeSCequipped

App

ID 1

6414 Chlorinated Pesticides: EPA Method 8081A

Column: Zebron ZB-XLB


Part No.: 7HG-G019-11

Injection: Split 111:1 @ 250 ˚C, 1.5 μL

Carrier Gas: Helium @ 0.9 mL/min (constant flow)

Oven Program: 110 ˚C to 320 ˚C at 15 ˚C and hold until last peak elutes

Detector: ECD @ 350 ˚C

Sample: 1. a-BHC 2. g-BHC 3. b-BHC 4. d-BHC 5. Heptachlor 6. Aldrin 7. Heptachlor epoxide 8. g-Chlordane 9. a-Chlordane 10. Endosulfan I

11. 4,4’-DDE 12. Dieldrin 13. Endrin 14. 4,4’-DDD 15. Endosulfan II 16. Endrin aldehyde 17. 4,4’-DDT 18. Endosulfan sulfate 19. Methoxychlor 20. Endrin ketone



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Zebron™ MultiResidue™ 1 & 2 • Novel phase designed for pesticides, herbicides, and insecticides • Designed for dual-column analysis of pesticide samples • Baseline resolution and confirmation of 20 chlorinated pesticides in < 10 min! • Low bleed performance for pesticide confirmation by MS


43

1

23

4

5

7

6

8 91011

1213

14

15161719

1820

21

2223

6 85 7 9 min

1

2

3

4

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76

8

9 101112

13 14

151617 19

18

20

21

2223

43 6 85 7 9 10 min

MR-2MR-1

Baseline Resolution in 10 Minutes!

If you need a 5 in. cage, simply add a (-B) after the part number.


ZB-MR-1ID(mm) df(µm) Temp. Limits °C Part No. Price30-Meter I

I I I 0.25 0.25 -60 to 320/340 7HG-G016-11 0.32 0.50 -60 to 320/340 7HM-G016-17 0.53 0.50 -60 to 320/340 7HK-G016-17

ZB-MR-2ID(mm) df(µm) Temp. Limits °C Part No. Price30-Meter I

I I I 0.25 0.20 -60 to 320/340 7HG-G017-10 0.32 0.25 -60 to 320/340 7HM-G017-11 0.53 0.50 -60 to 320/340 7HK-G017-17

Organochlorine Pesticides - Fast: EPA Method 8081 Part Nos.: 7HM-G016-17 (MR-1) 7HM-G017-11 (MR-2) Injection: Split @ 250 °C, 1 µL Carrier Gas: Helium @ 3.4 mL/min (constant flow) Oven Program: 100 °C for 0.5 min to 220 °C @ 35 °C/min to 340 °C @ 20 °C/min to for 2 min Detector: ECD @ 350 °C Sample: Performed using a 5 m Z-Guard™ Column and a 'Y' splitter.

App

ID 1

6153

, 161

54

1. TCMX (Surr) 2. 1-Bromo-2-Nitrobenzene (IS) 3. a-BHC 4. g-BHC (Lindane) 5. b-BHC 6. d-BHC 7. Heptachlor 8. Aldrin 9. Heptachlor Epoxide 10. trans-Chlordane (gamma) 11. cis-Chlordane (alpha)

12. Endosulfan I 13. 4, 4' -DDE 14. Dieldrin 15. Endrin 16. 4, 4' -DDD 17. Endosulfan II 18. Endrin Aldehyde 19. 4, 4' -DDT 20. Endosulfan Sulfate 21. Methoxychlor 22. Endrin Ketone 23. Decachlorobiphenyl (Surr)

Certified

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top Solutions for Pharmaceutical Laboratories

Zebron™ ZB-624 • Great separation of volatile organic flavor and fragrance additives and residual solvents in industrial or pharmaceutical products (OVIs) • Specifically designed for the separation of volatile organic compounds (VOCs) • Formulated for low bleed • Speed up run times and re-equilibration with high temperature limit • Equivalent to USP Phase G43


Alternative to Any 6 %-Cyanopropylphenyl- 94 %-Dimethylpolysiloxane Phase:DB-624 AT-624 DB-VRX Rtx-1301 CP-1301 SPB-624 Rtx-624

BP624 007-502 HP-VOC 007-624 Rtx-VMS DB-1301 SPB-1301

CP-Select 624 CB


I I

I 0.18 1.00 -20 to 260 7FD-G005-22 30-Meter0.25 1.40 -20 to 260 7HG-G005-27 0.32 1.80 -20 to 260 7HM-G005-31 0.53 3.00 -20 to 260 7HK-G005-36 60-Meter0.25 1.40 -20 to 260 7KG-G005-27 0.32 1.80 -20 to 260 7KM-G005-31 0.53 3.00 -20 to 260 7KK-G005-36 75-Meter0.53 3.00 -20 to 260 7LK-G005-36 105-Meter0.53 3.00 -20 to 260 7NK-G005-36 Test MixZebron ZB-624 AG0-5159

Conditions same for all runs

Column: Zebron ZB-624 Dimensions: 30 meter x 0.32 mm x 1.80 µmPart No.: 7HM-G005-31Injection: Split 5:1 @ 140 °C, 1 mLCarrier Gas: Helium @ 35 cm/sec (constant flow)Oven Program: 40 °C for 20 min to 240 °C @ 10 °C/min for 20 minDetector: FID @ 250 °C

USP Method <467> Residual Solvents Procedure A. A) Class 1 for water soluble compounds. B) Class 2 mix A for water soluble compounds. C) Class 2 mixB for water soluble compounds.

A Sample: 1. 1,1-Dichloroethene 2. 1,1,1-Trichloroethane 3. Carbon tetrachloride 4. Benzene 5. 1,2-Dichloroethane

4 6 8 10 12 min

APPID 16348

2

3

1

4

5

B Sample: 1. Methanol 2. Acetonitrile 3. Dichloromethane 4. cis-1,2-Dichloroethene 5. THF 6. Cyclohexane 7. Methylcyclohexane

10 155 20 25 30min

12

3

4

5

6 7

8

9 10

11

12,13

14

APPID 16349

8. 1,4-Dioxane 9. Toluene 10. Chlorobenzene 11. Ethylbenzene 12. m-Xylene 13. p-Xylene 14. o-Xylene

C Sample: 1. Hexane 2. Nitromethane 3. Chloroform 4. 1,2-Dimethoxyethane

5. Trichloroethylene 6. Pyridine 7. Methylbutylketone 8. Tetralin

10 20 30 min

APPID 16350

1

3

2

5

46

7

8


App

ID: 1

6348

App

ID: 1

6349

App

ID: 1

6350


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12

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7

8

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1112 13

16 19

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3334 36

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38 39

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48

45, 46

3524

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Zebron™ ZB-WAXPLUS™

• 100 % aqueous stable • Extremely inert for acidic compounds • Enhanced selectivity for low boiling solvents • High retention of alcohols and other chlorinated solvents • Solvent rinsible • Equivalent to USP Phase G16


Alternative to Any Polyethylene Glycol Phase:DB-WAX PEG 20M HP-20M Stabilwax®

Supelcowax-10 AT-WAX CP-WAX 52 CB AT-AquaWax

Carbowax-20M Permabond CW 20M CAM BP20

Solvents

Column: Zebron ZB-WAXPLUS Dimensions: 30 meter x 0.25 mm x 0.25 µmPart No.: 7HG-G013-11Injection: Split 50:1 @ 220 °C, 0.2 µLCarrier Gas: Helium @ 1.2 mL/min (constant flow)Oven Program: 30 °C for 1 min to 70 °C @ 14 °C/min to 220 °C @ 25 °C for 3 minDetector: MSD @ 250 °C; 18-350 amuSample: 1. Air

2. Pentane 3. Hexane 4. Ethyl ether 5. 2-Methoxy-2-methyl-2-propane (MTBE) 6. Heptane 7. Cyclohexane 8. 1,1-Dichloroethene 9. Methylcyclohexane 10. Acetone 11. Formic acid ethyl ester (Ethyl formate) 12. Acetic acid methyl ester (Methyl acetate) 13. Tetrahydrofuran (THF) 14. Carbon tetrachloride 15. 1,1,1-Trichloroethane 16. Ethyl acetate 17. Isopropyl acetate 18. 2-Butanone (MEK) 19. 1,2-Dimethoxyethane 20. Dichloromethane 21. Isopropanol 22. Benzene 23. Propyl acetate 24. 1,2-Dichloroethene 25. Trichloroethylene 26. Acetonitrile 27. Methylisobutylketone (MIBK) 28. Isobutyl acetate 29. Chloroform

30. Toluene 31. n-Propanol 32. Water 33. 1,4-Dioxane 34. 1,2-Dichloroethane 35. Butyl acetate 36. 2-Hexanone (MBK) 37. 2-Methylpropanol 38. Ethylbenzene 39. p-Xylene 40. m-Xylene 41. Butanol 42. Nitromethane 43. Cumene 44. 2-Methoxy ethanol 45. Pyridine 46. o-Xylene 47. 3-Methyl butanol 48. Chlorobenzene 49. n-Pentanol 50. DMF 51. Anisole 52. Tetralin 53. DMSO

54. Ethylene glycol (1,2-ethanediol) 55. Methylacetamide 56. n-Methylpyrrolidinone 57. Formamide


I I I 0.10 0.10 20 to 250/260 7CB-G013-02 15-Meter0.25 0.25 20 to 250/260 7EG-G013-11 20-Meter0.18 0.18 20 to 250/260 7FD-G013-08 30-Meter0.25 0.25 20 to 250/260 7HG-G013-11 0.32 0.25 20 to 250/260 7HM-G013-11 0.32 0.50 20 to 250/260 7HM-G013-17 0.53 1.00 20 to 230/240 7HK-G013-22 60-Meter0.25 0.25 20 to 250/260 7KG-G013-11 0.32 0.50 20 to 250/260 7KM-G013-17 0.53 1.00 20 to 230/240 7KK-G013-22 Test MixZebron ZB-WAXPLUS AG0-7869

App

ID 1

5926

If you need a 5 in. cage, simply add a (-B) after the part number, e.g., 7HG-G018-11-B.

*Thicker films (≥1.0 μm df) are rated to 230/240 ˚C (Isothermal/TPGC).


GC/MS analysis of class 1, 2, and 3 solvents



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10

Phenex™ Syringe Filters • Rapid sample and solvent filtration prior to chromatography • Particulate, PVC, and extractable-free filters • More consistent, reproducible results

Phenex™ SYRInGE FILtERS

Syringe Filter Selection Guide

3. Choose a filter membrane according to the characteristics of your sample and filtering objective Membrane Type Recommended Uses

RC(Regenerated Cellulose)

Hydrophilic Regenerated Cellulose filter membranes are compatible with a very broad range of aqueous and mixed-organic solutions, making them one of the most universal filter materials prior to chromatography. Phenex-RC filters also exhibit fast-flow and ultra-low protein and non-specific binding characteristics. Due to the beneficial material characteristics, Phenex-RC membranes are broadly recommended as an excellent general purpose/high-performance sample filter for most applications.

PTFE Teflon®

(Polytetrafluoroethylene) Phenex-PTFE membranes are inherently hydrophobic and excellent for filtration of 100 % organic or highly acidic or basic samples and solvents. Widely used in chromatography, it is especially well suited for the clarification of non-aqueous samples. Although this membrane is hydrophobic, it can be made hydrophilic by wetting the membrane with alcohol and then flushing with deionized water.

PES(Polyethersulfone)

Polyethersulfone membranes exhibit very fast-flow and ultra-low protein binding characteristics and are ideally suited for use in many life science clarification applications. Phenex-PES membranes typically offer better chemical resistance than cellulose acetate and are broadly recommended for filtering critical biological samples, tissue culture media, additives and buffers.

NY(Nylon)

Nylon has inherent hydrophilic characteristics and works well for filtration of many aqueous and mixed-organic sample types. For applications that require low protein or non-specific binding characteristics, Phenomenex recommends Phenex-RC (Regenerated Cellulose) filters.

CA(Cellulose Acetate)

Cellulose Acetate membranes exhibit ultra-low protein binding and are broadly used in the filtration of biological samples. In combination with a glass pre-filter (Phenex-GF/CA), this membrane is excellent for filtration of tissue culture media, general biological sample filtration and clarification.

GF(Glass Fiber)

Phenex-GF (Glass Fiber) filters are made of inert borosilicate glass and have a nominal 1.2 µm pore size. They are commonly used with highly viscous samples or samples that contain high concentrations of particulate matter (e.g., food analysis, biological samples, soil samples, fermentation broth samples, removal of yeasts, molds, etc.). Glass fiber filters can be used alone or in series with other Phenex filter membranes such as the 0.45 µm pore Phenex-RC filter to reduce clogging of the membrane and optimize flow.

1. Choose filter diameter based on sample volume

2. Choose pore size based on the nature of your sample and chromatographic methodSample Description Recommended Filter Pore Size

General aqueous or mixed organic samples prior to HPLC analysis with column packed with > 3 µm particles.General clarification of GC, SFC, CE, and GPC samples. 0.45 µm

Viscous samples or samples containing high levels of particulate matter.

General aqueous or mixed organic samples prior to HPLC analysis with columns packed with < 3 µm particles. Removal of fine particulate matter prior to GC, SFC, CE, and GPC samples. 0.20 µmGas samples prior to GC. Liquid samples prior to UHPLC or LC/MS. Other particulate-sensitive methods.

Viscous samples such as serum, plasma or other biological matrices. Solutions with high particulate load such as, some environmental or food and beverage applications.

In-line Glass Fiber Pre-Filter or Integrated Glass Fiber Filter Unit

Maximum chemical compatibility 100 % integrity tested Minimum extractables Low hold-up volume Excellent flow rate Low protein adsorption High total throughput Bi-directional use Certified quality

4 mm Diameter I 15 - 17 mm Diameter I 25 - 28 mm Diameter

≤ 2 mL Sample Volume 2 to 10 mL Sample Volume 10 to 100 mL Sample Volume

Phenex Offers:

Phenomenex | Web: www.phenomenex.com Acc

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Phenex™ SYRInGE FILtERS

For high load and particulate-laden samples you may consider placing a Glass Fiber (GF) pre-filter in line with the membrane syringe filter of your choice.

Phenex™ Syringe Filters (cont'd)

Above syringe filters are non-sterile. Housing is made of medical-grade polypropylene (PP). Luer lock inlet/slip outlet connections unless otherwise indicated.

1. 17 mm diameter.2. Glass fiber filters are 28 mm diameter and made of borosilicate. They will remove 90 % of all particles >1.2 µm. 3. Housing material is methacrylate butadiene styrene (MBS) polymerisate. Also known as Cyrolite™.4. Cellulose acetate is surfactant-free.5. 26 mm diameter.6. Hydrophobic membrane. Can be made hydrophilic by pre-wetting with IPA.7. 28 mm diameter.8. Additional dimensions and membrane types are available. Please contact your local Phenomenex technical consultant or distributor for availability or assistance.9. Larger quantity purchases at significant savings are available.

Application / Sample I Recommended Filter I First Alternative I Second Alternative

HPLC and GC Sample Prep RC PTFE PES Aggressive or Pure Organic Solvents PTFE RC NY Protein Analysis/ Biological Samples PES RC GF/CA High Particulate Loads RC NY PTFE Environmental Methods RC PTFE NY Food and Beverage RC PTFE NY Clinical / Toxicology RC PES NY Dissolution Testing RC PTFE NY Ion Chromatography RC PES PTFE Trace Metals (ICP-MS, AAS) RC PES NY Capillary Electrophoresis (CE) RC PES NY Tissue Cultures, Media, Buffers GF/CA PES RC

Syringe Filter Applications and Recommended Membranes


Phenex Syringe Filters 4 mm Diameter 15 mm Diameter 25 mm Diameter for ≤ 2 mL sample volumes for 2 - 10 mL sample volumes for 10 - 100 mL sample volumes Membrane Type/Size Part No. Unit Price Part No. Unit Price Part No. Unit Price 0.20 µm Phenex-RC AF0-3203-12 100/Pk AF0-2203-12 100/Pk AF0-8203-12 5 100/Pk (Regenerated Cellulose) AF0-3203-52 500/Pk AF0-2203-52 500/Pk AF0-8203-52 5 500/Pk Phenex-PES 3 — — — — — — AF0-8208-12 7 100/Pk (Polyethersulfone) — — — — — — AF0-8208-52 7 500/Pk Phenex-PTFE 6 AF0-3202-12 100/Pk AF0-2202-12 100/Pk AF0-1202-12 100/Pk (Polytetrafluoroethylene) AF0-3202-52 500/Pk AF0-2202-52 500/Pk AF0-1202-52 500/Pk Phenex-NY AF3-3207-12 100/Pk AF2-5207-12 1 100/Pk AF0-1207-12 100/Pk (Nylon) AF3-3207-52 500/Pk AF2-5207-52 1 500/Pk AF0-1207-52 500/Pk Phenex-GF/CA 2,3,4 An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a CA membrane. AF0-8A09-12 7 100/Pk (Glass Fiber/Cellulose Acetate) Excellent for filtration of tissue culture media, general biological sample filtration and clarification. AF0-8A09-52 7 500/Pk Outlet connection is luer lock. 0.45 µm Phenex-RC AF0-3103-12 100/Pk AF0-2103-12 100/Pk AF0-8103-12 5 100/Pk (Regenerated Cellulose) AF0-3103-52 500/Pk AF0-2103-52 500/Pk AF0-8103-52 5 500/Pk Phenex-PES 3 — — — AF2-5108-12 1 100/Pk AF0-8108-12 7 100/Pk (Polyethersulfone) — — — — — — AF0-8108-52 7 500/Pk Phenex-PTFE 6 AF0-3102-12 100/Pk AF0-2102-12 100/Pk AF0-1102-12 100/Pk (Polytetrafluoroethylene) AF0-3102-52 500/Pk AF0-2102-52 500/Pk AF0-1102-52 500/Pk Phenex-NY AF3-3107-12 100/Pk AF2-5107-12 1 100/Pk AF0-1107-12 100/Pk (Nylon) AF3-3107-52 500/Pk AF2-5107-52 1 500/Pk AF0-1107-52 500/Pk Phenex-GF/CA 2,3,4 An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a CA membrane. AF0-8B09-12 7 100/Pk (Glass Fiber/Cellulose Acetate) Excellent for filtration of tissue culture media, general biological sample filtration and clarification. AF0-8B09-52 7 500/Pk Outlet connection is luer lock. 1.20 µm Phenex-GF 2,3 Prefiltration of heavily contaminated or highly viscous samples. When used in-line preceding AF0-8515-12 7 100/Pk (Glass Fiber) a membrane filter, clogging of the membrane filter is prevented and sample clean up is optimized. AF0-8515-52 7 500/Pk Outlet connection is luer lock.


HP

LC S

ecurityGuard

™

12

SecurityGuard™

• Extends your HPLC column lifetime • Won’t alter your chromatography • Compatible with virtually any HPLC column • Easy to use

SecurityGuard can protect valuable HPLC columns from the damaging effects of chemical contaminants and microparticulates, without altering chromatographic results. This protection can lead to an extension of the column life. Simply put, every chromatographer must have one.

Does Not Alter Your Chromatography

WITH SecurityGuard WITHOUT SecurityGuard

Compare, Virtually No Change!vs.0

0

100

200

300

400

500

mAU

5 20 25 30 min10 15

0

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5 20 25 30 min10 15

UnIVERSAL GUARD CARtRIDGE SYStEM

0

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5 20 25 30 min10 15

0

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300

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5 20 25 30 min10 15

Phenomenex Luna®

Waters Symmetry®

Thermo Fisher HYPERSIL®

Alltech Alltima™

It’s a Universal Fit*

SecurityGuard will fit virtually ANY manufacturer’s HPLC column, making it truly a universal guard system.

*patented

Universal Fit* Floating outlet fits ANY manufacturer’s female/inverted endfitting. Feature applies to analytical-sized guard systemonly, and does not apply to semi-prep or PREPguard cartridges.

Contaminants Trapped

Compounds of InterestCompounds of Interest

ContaminantsContaminants

HP

LC C

olumn

From Injector

Guard

Cartrid

ge System

Cutaway view showingCartridge – can be easily inspected for contaminants

Universal fingertight connection to HPLC column - no wrenches required

One Holder for All

How it Works


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SecurityGuard™

UnIVERSAL GUARD CARtRIDGE SYStEM

2.0-3.0

4.0 x 2.0

3.2-8.0

4.0 x 3.0

9.0-16.0

10.0 x 10.0 15.0 x 21.2 15.0 x 30.0

18.0-29.0 30.0-49.0

ANALYTICAL SEMI-PREP PREP

If your HPLC column ID (mm) is:

Use Cartridges (mm):

Material Description pH Stability

Cartridges for General Purpose/Pharmaceutical /10pk /10pk /3pk /ea /ea

C18 (ODS, Octadecyl) 1.5 - 10 AJ0-4286 AJ0-4287 AJ0-7221 AJO-7839 AJO-8301

C12 (Dodecyl) 1.5 - 10 AJ0-6073 AJ0-6074 AJ0-7275 AJO-7842 AJO-8304

C8 (MOS, Octyl) 1.5 - 10 AJ0-4289 AJ0-4290 AJ0-7222 AJO-7840 AJO-8302

C5 (Pentyl) 1.5 - 10 AJ0-4292 AJ0-4293 AJ0-7372 — —

C1 (TMS) 2 - 9 AJ0-4298 AJ0-4299 AJ0-7373 — —

Silica — — AJ0-4347 AJ0-4348 AJ0-7223 AJO-7229 AJO-8312

HILIC HILIC 1.5 - 8 AJ0-8328 AJ0-8329 — — —

NH2 (Amino, Aminopropyl) 1.5 - 11 AJ0-4301 AJ0-4302 AJ0-7364 AJ0-8162 AJO-8309

CN (Cyano, Cyanopropyl) 2 - 7.5 AJ0-4304 AJ0-4305 AJ0-7313 AJ0-8220 AJO-8311

Phenyl (Phenylhexyl) 1.5 - 10 AJ0-4350 AJ0-4351 AJ0-7314 AJO-7841 AJO-8303

PFP(2) (pentafluorophenylpropyl) 1.5 - 8 AJ0-8326 AJ0-8327 AJ0-8376 AJ0-8377 AJ0-8378

SCX (SA, Strong Cation Exchanger) 2.5 - 7.5 AJ0-4307 AJ0-4308 AJ0-7369 — —

SAX (SB, Strong Anion Exchanger) 2.5 - 7.5 AJ0-4310 AJ0-4311 AJ0-7370 — —

RP-1 (Reversed Phase - Polymer) 0 - 14 AJ0-5808 AJ0-5809 AJ0-7368 AJO-8358 —

Polar-RP (Ether-linked Phenyl) 1.5 - 7 AJ0-6075 AJ0-6076 AJ0-7276 AJO-7845 AJO-8307

Fusion-RP (C18 Polar Embedded) 1.5 - 10 AJ0-7556 AJ0-7557 AJ0-7558 AJO-7844 AJO-8306

AQ C18 (Polar Endcapped C18) 1.5 - 7.5 AJ0-7510 AJ0-7511 AJ0-7512 AJO-7843 AJO-8305

Gemini®-NX (C18 TWIN™ Technology) 1 - 12 AJ0-8367 AJ0-8368 AJ0-8369 AJ0-8370 AJ0-8371

Gemini® C18 (C18 TWIN™ Technology) 1 - 12 AJ0-7596 AJ0-7597 AJ0-7598 AJO-7846 AJO-8308

Gemini® C6-Phenyl (C6-Phenyl TWIN Technology) 1 - 12 AJ0-7914 AJ0-7915 — — —

Oligo-RP (C18 TWIN Technology) 1 - 12 AJ0-8134 AJ0-8135 AJ0-8136 AJ0-8210 AJ0-8310

Oligo-WAX (WA, Weak Anion Exchanger) 1.5 - 11 — AJ0-8324 AJ0-8325 — —

Cartridges for Protein and Polypeptide Reversed Phase /10pk /10pk /3pk /ea /ea

For use with silica columns for separation of proteins & peptides, such as Jupiter® (Phenomenex); Vydac® 218TP, 214TP (Alltech Associates, Inc.); SynChropak® 300 C18, C4 (Eprogen, Inc.); Nucleosil® 300 Å C18, C4; HyPErSIL® 300 Å, and other widepore or 300 Å brands.

Widepore C18 (ODS, Octadecyl) 1.5 - 10 AJ0-4320 AJ0-4321 AJ0-7224 AJO-7230 AJO-8313

Widepore C5 (Pentyl) 1.5 - 10 AJ0-4326 AJ0-4327 AJ0-7371 — —

Widepore C4 (Butyl) 1.5 - 10 AJ0-4329 AJ0-4330 AJ0-7225 AJO-7231 AJO-8314

Cartridges for Silica GFC — /10pk /3pk — —(Aqueous SEC) For use with silica GFC columns, such as BioSep™ (Phenomenex); ZorBAx® GF-Series; Bio-Sil® (Bio-rad).

GFC-2000 – 2 - 7.5 — AJ0-4487 AJ0-7365 — —

GFC-3000 – 2 - 7.5 — AJ0-4488 AJ0-7366 — —

GFC-4000 – 2 - 7.5 — AJ0-4489 AJ0-7367 — —

Cartridges for Chiral /10pk /10pk /3pk /ea /ea

For use with chiral columns, such as Lux™ (Cellulose-1 & Cellulose-2 (Phenomenex); CHIrALCEL® oD-H® (DAICEL Chemical industries LTD).

Lux™ Cellulose-1

(Cellulose tris (3, 5-dimethylphenyl-carbamate)

2 - 8 AJ0-8402 AJ0-8403 AJ0-8404 AJ0-8405 AJ0-8406

Lux™ Cellulose-2

(Cellulose tris (3-chloro-4-methyl-phenylcarbamate)

2 - 8 AJ0-8398 AJ0-8366 AJ0-8399 AJ0-8400 AJ0-8401

Cartridges for Carbohydrate/Organic Acid /10pk

For organic acid and carbohydrate analysis, such as rezex™ (Phenomenex); Aminex® (Bio-rad); Interaction; Sugar-Pak™ (Waters).

Carbo-H+ – 1.0 - 7.5 — AJ0-4490 — — —

Carbo-Ag+* – Neutral — AJ0-4491 — — —

Carbo-Pb+2 – Neutral — AJ0-4492 — — —

Carbo-Ca+2 – Neutral — AJ0-4493 — — —

* For use with saccharide and oligosaccharide columns in Ag+ form.

Guard Cartridge Holders (one-time purchase only) /kit /holder /kit /kit

KJ0-4282 AJ0-7220 AJO-8223 AJO-8277



Accessories

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Gemini®-nX C18 • Control sensitivity and peak purity • Robust method transfers • Decrease purification time

HIGH PERFORMAnCE pH StABLE HPLC COLUMnS

APP

ID 1

6601

pH 7.0 pH 10.5

APP

ID 1

6600 Column: Gemini-NX 5 µm C18

Dimensions: 150 x 4.6 mmPart No.: 00F-4454-E0

Mobile Phase: A: 20 mM Potassium Phosphate pH 7.0B: Acetonitrile

Gradient: A/B (95:5) to (5:95) in 10 min, Hold for 2 min

Flow Rate: 1.5 mL/minTemperature: Ambient

Detection: UV @ 254 nmSample: 1.

2.3.

Amitriptyline Naproxen Toluene

Column: Gemini-NX 5 µm C18Dimensions: 150 x 4.6 mm

Part No.: 00F-4454-E0Mobile Phase: A: 10 mM Ammonium

Bicarbonate pH 10.5B: Acetonitrile




2.3.

Amitriptyline NaproxenToluene

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APP

ID 1



Mobile Phase: A: 0.1 % Formic Acid in WaterB: 0.1 % Formic Acid in Acetonitrile




2.3.


pH 4.8

APP

ID 1



Mobile Phase: A: 10 mM Ammonium Acetate pH 4.8B: Acetonitrile




2.3.


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1 2

3

O

SiCH2

CH2

O

O

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Si S

O

O

Si

O O

O

O

Si

O

O Si Si

O

O

Si

O

O

O

Si Si

O

Si SiOOCH2

CH2

CH2

CH2

CH2

CH2

CH2

CH2

CH2

CH2

C

C

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SiCH2

CH2

O

O

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Si Si

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Si

O O

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O Si Si

O

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O

Si Si

O

Si SiOOCH2

CH2

CH2

CH2

CH2

CH2

CH2

CH2

CH2

CH2

CH2

CH2

C

Second-Generation TWIN-NX™ TechnologyGemini-NX uses an improved patent pending organo-silica grafting process which incorporates highly stabilizing ethane cross-linking. These organic groups are evenly incorporated into the grafted layers on the silica surface while maintaining a pure silica core. This not only provides resistance to high pH attack, but also maintains the high efficiency and mechanical strength of a silica particle.

The uniform TWIN-NX grafting technology presents an ordered surface silane distribution. This provides homogeneously active bonding sites ideal for low pH resistant, multi-point ligand attachments.

Create Compound Optimized Methods Using pH

new

Gemini-NX is Patent Pending.


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HIGH PERFORMAnCE pH StABLE HPLC COLUMnS

For more information about SecurityGuard see pp. 12-13

Gemini®-nX C18 (cont’d)

Excellent Performance in Volatile Buffers!

APP

ID 1

6618 Gemini®-NX 5 µm C18

0 2 4 6 8 10 min

1

2

3

4

5

6

7

8

9

10Excellent Performance in

Non-Volatile Buffers!

APP

ID 1

6616 Gemini®-NX 5 µm C18

0 2 4 6 8 10 12 min

1

2

3

4

5

6

78

9

10

Mixtures of Acids, Neutrals, and Bases in Formic Acid pH 2.7


Part No.: 00F-4454-E0Mobile Phase: A: 0.1 % Formic Acid in Water

B: 0.1 % Formic Acid in AcetonitrileGradient: A/B (95:5) to (20:80) in 8 min,

Hold for 2 minFlow Rate: 1.5 mL/min

Temperature: AmbientDetection: UV @ 254 nm

Sample: 1.2.3.4.5.6.7.8.9.

10.

PyridineQuinidineSulfathiazoleTriprolidineBenzyl alcoholNortriptylinePhenol3-Methyl-4-nitrobenzoic acidMethylsalicylaldehydeHexanophenone

Mixtures of Acids, Neutrals, and Bases in Potassium Phosphate pH 2.5


Part No.: 00F-4454-E0Mobile Phase: A: 20 mM Potassium Phosphate pH 2.5

B: AcetonitrileGradient: A/B (95:5) to (20:80) in 8 min,

Hold for 2 minFlow Rate: 1.5 mL/min

Temperature: AmbientDetection: UV @ 254 nm

Sample: 1.2.3.4.5.6.7.8.9.

10.

PyridineQuinidineSulfathiazoleTriprolidineBenzyl alcoholPhenol Nortriptyline3-Methyl-4-nitrobenzoic acidMethylsalicylaldehydeHexanophenone

Robust Method Transfers

10 μm Axia™ Packed Preparative Columns (mm) continued SecurityGuard™Cartridges (mm)Phases 250 x 30 50 x 50 100 x 50 150 x 50 250 x 50 15 x 21.2** 15 x 30.0

/ea /ea

C18 00G-4455-U0-AX 00B-4455-V0-AX 00D-4455-V0-AX 00F-4455-V0-AX 00G-4455-V0-AX AJ0-8370 AJ0-8371

for ID: 18-29 mm 30-49 mm

5 μm Axia™ Packed Preparative Columns (mm) continued SecurityGuard™ Cartridges (mm)Phases 75 x 30 100 x 30 150 x 30 250 x 30 50 x 50 15 x 21.2** 15 x 30.0

/ea /ea

C18 00C-4454-U0-AX 00D-4454-U0-AX 00F-4454-U0-AX 00G-4454-U0-AX 00B-4454-V0-AX AJ0-8370 AJ0-8371

for ID: 18.0-29 mm 30-49 mm

10 μm Axia™ Packed Preparative Columns (mm) SecurityGuard™Cartridges (mm)Phases 50 x 21.2 100 x 21.2 150 x 21.2 250 x 21.2 100 x 30 150 x 30 15 x 21.2** 15 x 30.0

/ea /ea

C18 00B-4455-P0-AX 00D-4455-P0-AX 00F-4455-P0-AX 00G-4455-P0-AX 00D-4455-U0-AX 00F-4455-U0-AX AJ0-8370 AJ0-8371

for ID: 18-29 mm 30-49 mm

3 μm Minibore Columns (mm) SecurityGuard™ Cartridges (mm)Phases 20 x 2.0 30 x 2.0 50 x 2.0 100 x 2.0 150 x 2.0 4 x 2.0*

/10pkC18 00M-4453-B0 00A-4453-B0 00B-4453-B0 00D-4453-B0 00F-4453-B0 AJ0-8367

for ID: 2.0-3.0 mm

5 μm Minibore Columns (mm) SecurityGuard™ Cartridges (mm)Phases 30 x 2.0 50 x 2.0 100 x 2.0 150 x 2.0 4 x 2.0*

/10pk

C18 00A-4454-B0 00B-4454-B0 00D-4454-B0 00F-4454-B0 AJ0-8367

for ID: 2.0-3.0 mm

3 μm Analytical Columns (mm) SecurityGuard™ Cartridges (mm)Phases 30 x 3.0 50 x 3.0 100 x 3.0 150 x 3.0 50 x 4.6 100 x 4.6 150 x 4.6 250 x 4.6 4 x 2.0* 4 x 3.0*

/10pk /10pk

C18 00A-4453-Y0 00B-4453-Y0 00D-4453-Y0 00F-4453-Y0 00B-4453-E0 00D-4453-E0 00F-4453-E0 00G-4453-E0 AJ0-8367 AJ0-8368

for ID: 2.0-3.0 mm 3.2-8.0 mm

5 μm Axia™ Packed Preparative Columns (mm) SecurityGuard™ Cartridges (mm)Phases 50 x 21.2 100 x 21.2 150 x 21.2 250 x 21.2 50 x 30 15 x 21.2** 15 x 30.0

/ea /ea

C18 00B-4454-P0-AX 00D-4454-P0-AX 00F-4454-P0-AX 00G-4454-P0-AX 00B-4454-U0-AX AJ0-8370 AJ0-8371

for ID: 18.0-29 mm 30-49 mm

5 μm Analytical Columns (mm) SecurityGuard™ Cartridges (mm)Phases 50 x 3.0 100 x 3.0 150 x 3.0 250 x 3.0 50 x 4.6 75 x 4.6 100 x 4.6 150 x 4.6 250 x 4.6 4 x 2.0* 4 x 3.0*

/10pk /10pk

C18 00B-4454-Y0 00D-4454-Y0 00F-4454-Y0 00G-4454-Y0 00B-4454-E0 00C-4454-E0 00D-4454-E0 00F-4454-E0 00G-4454-E0 AJ0-8367 AJ0-8368

for ID: 2.0-3.0 mm 3.2-8.0 mm

*SecurityGuard™ Analytical Cartridges require holder, Part No.: KJ0-4282**PREP SecurityGuard™ Cartridges require holder, Part No.: AJ0-8223♦PREP SecurityGuard™ Cartridges require holder, Part No.: AJ0-8277


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TM

Onyx™ Analytical & Semi-Prep Monolithic Columns • 2 mm ID column for improved sensitivity and less solvent usage • Reduce run times by more than 50 % • Eliminate time consuming sample preparation by using “dilute-and-shoot” methods • Extremely low backpressures preserves column and system lifetime

Traditional Silica “Particle-Based” Column

• Individual silica particles

• High flow resistance: Limits ability to shorten run times

• High backpressure: Reduces life of pumps, seals, and column

• Reduced throughput: Long run times

• Bed splitting possible: Shortens column life and lessens reproducibility

“Monolithic” Column

• Monolithic porous silica rod

• High flow rates: Due to high porosity

• Low backpressure: Less stress on system and column

• Increased throughput: Significantly shorter run times

• No inlet bed settling: Increased reliability, reproducibility, and lifetime


Monolithic Silica HPLC ColumnsAnalytical ColumnsPart No. Description Size (mm) Price

CH0-8373 Onyx Monolithic C18 50 x 2.0






CH0-7648 Onyx Monolithic Si 100 x 4.6

SemiPrep ColumnsPart No. Description Size (mm) Price

CH0-7878 Onyx SemiPrep Monolithic C18 100 x 10.0

FASt HIGH-tHROUGHPUt SEPARAtIOnS


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App ID: 15585

mAU

Onyx™ Capillary Columns • Ideal for peptide mapping • Load samples at µL/min flow rates to significantly decrease cycle time • Trap loading unnecessary due to minimal carryover • Long lifetime when working with biological samples

TM

Tryptic Digest of b-Amylase

b-Amylase is digested by Trypsin to generate a complex peptide map. No desalting or trap column is used, demonstrating Onyx’s resistance to matrix contaminants.

• The monolithic phase of Onyx delivers high peak efficiency and good peak shape even in a capillary format.

• Onyx monolithic silica allows for wide flow rate flexibility resulting in loading at 10 µL/min flow rates. A 20 µL sample can be directly loaded in less than 2 minutes without the use of a trap column.


Monolithic Silica HPLC Columns

Capillary Columns

Part No. Description Size (mm) Price



CH0-8389 Onyx Monolithic HD-C18 150 x 0.1


CH0-8391 Onyx Monolithic HD-C18 150 x 0.2

CH0-8392 Onyx Monolithic C18 Trapping Column 150 x 0.2


Conditions: Column: Onyx Monolithic C18 Dimensions: 150 x 0.1 mm Part No.: CH0-7646 Mobile Phase: A: 0.1 % TFA in Water B: 0.085 % TFA in Water / 95 % Acetonitrile Gradient: 5-65 % B in 60 minutes Flow Rate: 3.0 µL/min Temperature: Ambient Detection: UV @ 210 nm Sample: b-Amylase Tryptic Digest

FASt HIGH-tHROUGHPUt SEPARAtIOnS

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Synergi™ 4 µm Method Development Columns • Simplified column selection • High efficiency at low backpressures • Hydrophobic/philic and aromatic retention

Four Unique Phases to Advance Your LC Selectivity

ORDERING INFORMATION 4 µm Microbore Columns (mm)Phase 50 x 1.0 150 x 1.0 250 x 1.0

Max-RP 00B-4337-A0 00F-4337-A0 —

Hydro-RP 00B-4375-A0 00F-4375-A0 00G-4375-A0

Polar-RP 00B-4336-A0 00F-4336-A0 —

Fusion-RP 00B-4424-A0 00F-4424-A0 —SecurityGuard™ Cartridges require universal holder Part No.: KJ0-4282

4 µm Minibore and Narrow Bore Columns (mm) SecurityGuard™ CartridgesPhase 50 x 2.0 150 x 2.0 50 x 3.0 150 x 3.0 250 x 3.0 4 x 2.0 mm

/10pk

Max-RP 00B-4337-B0 00F-4337-B0 00B-4337-Y0 00F-4337-Y0 00G-4337-Y0 AJ0-6073

Hydro-RP 00B-4375-B0 00F-4375-B0 00B-4375-Y0 00F-4375-Y0 00G-4375-Y0 AJ0-7510

Polar-RP 00B-4336-B0 00F-4336-B0 00B-4336-Y0 00F-4336-Y0 00G-4336-Y0 AJ0-6075

Fusion-RP 00B-4424-B0 00F-4424-B0 00B-4424-Y0 00F-4424-Y0 00G-4424-Y0 AJ0-7556

for ID: 2.0-3.0 mm

4 µm Analytical Columns (mm) SecurityGuard™ CartridgesPhase 30 x 4.6 50 x 4.6 75 x 4.6 150 x 4.6 250 x 4.6 4 x 3.0 mm

/10pk

Max-RP 00A-4337-E0 00B-4337-E0 00C-4337-E0 00F-4337-E0 00G-4337-E0 AJ0-6074

Hydro-RP 00A-4375-E0 00B-4375-E0 00C-4375-E0 00F-4375-E0 00G-4375-E0 AJ0-7511

Polar-RP 00A-4336-E0 00B-4336-E0 00C-4336-E0 00F-4336-E0 00G-4336-E0 AJ0-6076

Fusion-RP 00A-4424-E0 00B-4424-E0 00C-4424-E0 00F-4424-E0 00G-4424-E0 AJ0-7557

for ID: 3.2-8.0 mm

4 µm Axia™ Packed Columns (mm) SecurityGuard™ CartridgesPhase 50 x 21.2 100 x 21.2 150 x 21.2 250 x 21.2 100 x 30 15 x 21.2** mm 15 x 30.0♦ mm

Max-RP 00B-4337-P0-AX 00D-4337-P0-AX 00F-4337-P0-AX 00G-4337-P0-AX 00D-4337-U0-AX AJ0-7842 AJ0-8304

Hydro-RP 00B-4375-P0-AX 00D-4375-P0-AX 00F-4375-P0-AX 00G-4375-P0-AX 00D-4375-U0-AX AJ0-7843 AJ0-8305

Polar-RP 00B-4336-P0-AX 00D-4336-P0-AX 00F-4336-P0-AX 00G-4336-P0-AX 00D-4336-U0-AX AJ0-7845 AJ0-8307

Fusion-RP 00B-4424-P0-AX 00D-4424-P0-AX 00F-4424-P0-AX 00G-4424-P0-AX 00D-4424-U0-AX AJ0-7844 AJ0-8306

for ID: 18-29 mm 30-49 mm

SIMPLIFIED HPLC MEtHOD DEVELOPMEnt

Advancing LC Selectivity

Hydrogen Bonding

Silanol ActivityMultiplied by 2

MethyleneSelectivityMultiplied by 3

Polar SelectivityHydrophobicityDivided by 5

0.800

0.700

0.600

0.500

0.400

0.300

0.200

0.100

0.000

Columns: Synergi 4 µm Fusion-RP Synergi 4 µm Max-RP Synergi 4 µm Hydro-RP Synergi 4 µm Polar-RPDimensions: 150 x 4.6 mm Mobile Phase: 1. Water / Methanol (20:80) for k Amylbenzene

2. Water / Methanol (20:80) for a (CH2) 3. Water / Methanol (70:30) for a C/P 4. Methanol / 20 mM Potassium phosphate pH 2.7 for a B/P, pH 2.75. 5. Methanol /20 mM Potassium phosphate pH 7.6 for a B/P, pH 7.6

Flow Rate: 1.0 mL/minDetection: UV @ 254 nmInjection: 5 mL Temperature: 30 °CSample: 1. Amylbenzene

2. Amylbenzene and Butylbenzene 3. Caffeine and phenol (C/P) 4. Benzylamine and phenol (B/P) @ pH 2.75 5. Benzylamine and phenol (B/P) @ pH 7.6

Synergi Fusion-RPSynergi Max-RPSynergi Hydro-RPSynergi Polar-RP

**PREP SecurityGuard™ Cartridges require holder, Part No.: AJ0-8223♦PREP SecurityGuard™ Cartridges require holder, Part No.: AJ0-8277


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*4 μm Synergi materials available upon request. ** Requires MercuryMS Cartridge Holder

0.5 1.0 1.5 2.0 min0

50

100

150

200

250

300

350

400

Intensity,cps

0.2 0.4 0.6 0.8 1.0 1.2 1.4 min0

100

200

300

400

500

600

700

800

Intensity,cps

0.5 1.0 1.5 2.0 min0

50

100

150

200

250

300

350

400

Intensity,cps

0.2 0.4 0.6 0.8 1.0 1.2 1.4 min0

100

200

300

400

500

600

700

800

Intensity,cps

Synergi™ 2.5 µm MercuryMS™

• Perform ultra-fast separations on your HPLC • High efficiency in small format • Achieve 2x increase in sensitivity

™

Synergi Polar-RP 4 μm 50 x 2.0 mm

1 mL/min, 100 bar 2.1 min run time

Synergi Polar-RP 2.5 μm 20 x 2.0 mm

1 mL/min, 220 bar 1.3 min run time

Data courtesy of GSK 2005

SIMPLIFIED HPLC MEtHOD DEVELOPMEnt

Same LC/MS

Advancing LC Selectivity


• 100 % Sensitivity gain • 30 % Reduction in run time

Cartridges (mm)** Columns (mm)Phase 10 x 2.0 10 x 4.0 20 x 20 20 x 4.0 20 x 2.0 20 x 4.0

2.5 μm PhaseSynergi Max-RP* 00N-4372-B0-CE 00N-4372-D0-E0 00M-4372-B0-CE 00M-4372-D0-CE 00M-4372-B0 00M-4372-D0

Synergi Hydro-RP* 00N-4387-B0-CE 00N-4387-D0-E0 00M-4387-B0-CE 00M-4387-D0-CE 00M-4387-B0 00M-4387-D0

Synergi Polar-RP* — 00N-4371-D0-CE 00M-4371-B0-CE 00M-4371-D0-CE 00M-4371-B0 00M-4371-D0

Synergi Fusion-RP* 00N-4423-B0-CE 00N-4423-D0-CE 00M-4423-B0-CE 00M-4423-D0-CE 00M-4423-B0 00M-4423-D0


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HSt: High Speed technology • Ultra-fast results on your current HPLC system • Easy method transfer

HIGH SPEED tECHnOLOGY

High Speed Technology

Synergi 4 µm Polar-RP Synergi 2.5 µm Polar-RP-HST

1

0 1.0 min

0 10 20 min

0 10 20 30 min

23

4

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7 8 9

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7 8 9

1

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23

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7 8 9

1

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0 10 20 30 min

23

4

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7 8 9

12 3

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7 8 9

Column: Synergi 2.5 µm Polar-RP-HSTDimensions: 50 x 2.0 mmPart No.: 00B-4371-B0 Mobile Phase: A: Water

B: AcetonitrileGradient: 10 % B hold for 0.5 min, to 95 % B

in 1.5 min, hold for 1 min, then to 10 % B in 0.1 min (or 1 sec.)

Flow Rate: 1.2 mL/minDetection: UV @ 270 nm; 1.9 min

re-equilibrationInjection: 1 µL Temperature: 60 °CSample: 20 mg/mL in Acetonitrile

1. Acetone 2. 2-Butanone 3. 2-Pentanone 4. 2-Hexanone 5. 2-Heptanone 6. 2-Octanone 7. 2-Decanone 8. 2-Tridecanone 9. 2-Hexadecanone

10 x Increase in speed

!

Column: Synergi 4 µm Polar-RPDimensions: 250 x 4.6 mmPart No.: 00G-4336-E0 Mobile Phase: A: Water

B: AcetonitrileGradient: 10 % B hold for 1 min, to 95 % B

in 25 min, hold for 10 min, then to 10 % B in 0.1 min

Flow Rate: 1.5 mL/minDetection: UV @ 270 nm; 9.9 min

re-equilibrationInjection: 5 µLTemperature: 60 °CSample: 20 mg/mL in Acetonitrile

1. Acetone 2. 2-Butanone 3. 2-Pentanone 4. 2-Hexanone 5. 2-Heptanone 6. 2-Octanone 7. 2-Decanone 8. 2- Tridecanone 9. 2-Hexadecanone

Synergi 4 µm Polar-RP250 x 4.6 mm

App ID 16263

App ID 16262

Synergi 2.5 µm Polar-RP-HST50 x 2.0 mm


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ORDERInG InFORMAtIOn

High Speed technology (HSt) Columns (mm)

30 x 2.0 50 x 2.0 100 x 2.0 50 x 3.0 100 x 3.0 50 x 4.6

Phases

Luna 2.5 µm C18(2)-HST 00A-4446-B0 00B-4446-B0 00D-4446-B0 00B-4446-Y0 00D-4446-Y0 ––

Alternative Selectivities

Synergi 2.5 µm Max-RP 00A-4372-B0 00B-4372-B0 00D-4372-B0 00B-4372-Y0 00D-4372-Y0 00B-4372-E0

Synergi 2.5 µm Polar-RP 00A-4371-B0 00B-4371-B0 00D-4371-B0 00B-4371-Y0 00D-4371-Y0 00B-4371-E0

Synergi 2.5 µm Hydro-RP 00A-4387-B0 00B-4387-B0 00D-4387-B0 00B-4387-Y0 00D-4387-Y0 00B-4387-E0

Synergi 2.5 µm Fusion-RP 00A-4423-B0 00B-4423-B0 00D-4423-B0 00B-4423-Y0 00D-4423-Y0 00B-4423-E0

HIGH SPEED tECHnOLOGY

High Speed Technology

HSt Columns• High performance efficiencies comparable to sub-2 µm particle size columns at roughly half the backpressure

0

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Flow Rate: 0.55 mL/min

0

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5

0.4 0.8 min

Luna 2.5 µm C18(2)-HST

254 bar

Competitor A 1.7 µm C18


Competitor A 1.7 µm C18Flow Rate: 0.55 mL/min

0

1

23 4

5

0.4 0.8 min

400 bar

Linear Velocity (mm/s)


400 bar

0.20

1

23 4

5

0.4 0.6 min


Competitor A 1.7 µm C18Flow Rate: 1.00 mL/min

0

2

1

3 45

0.2 0.4 0.6 min

850 bar

Conditions: (same for both columns) Columns: Luna 2.5 µm C18(2)-HST Competitor A 1.7 µm C18 Dimension: 50 x 2.0 mm (Luna) 50 x 2.1 mm (Competitor A) Mobile Phase: Acetonitrile / Water (65:35) Flow Rate: As noted Detection: UV @ 254 nm Temperature: 30 ºC Sample: 1. Uracil 2. Acetophenone 3. Benzene 4. Toluene 5. Naphthalene

To learn more about Synergi phases, see page 18 of this guide or visit www.phenomenex.com

APP

ID 1

6271

APP

ID 1

6272

APP

ID 1

6269

APP

ID 1

6270


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OUR tOP SELLInG HPLC COLUMn

Luna®

• Easy method scalability with 2.5, 3, 5, 10, 10-PREP, and 15 µm medias • Extensive batch traceability and reproducibility data supplied with every column • Exceptionally rugged USP phases


3 µm Microbore and Minibore Columns (mm) SecurityGuard™ CartridgesPhase 30 x 1.0 50 x 1.0 150 x 1.0 30 x 2.0 50 x 2.0 100 x 2.0 150 x 2.0 4 x 2.0 mm SecurityGuard

Cartridges require universal holder

Part No.: KJO-4282

/10pk

Silica(2) — — — 00A-4162-B0 00B-4162-B0 00D-4162-B0 00F-4162-B0 AJ0-4347

C8(2) 00A-4248-A0 00B-4248-A0 00F-4248-A0 00A-4248-B0 00B-4248-B0 00D-4248-B0 00F-4248-B0 AJ0-4289

C18(2) 00A-4251-A0 00B-4251-A0 00F-4251-A0 00A-4251-B0 00B-4251-B0 00D-4251-B0 00F-4251-B0 AJ0-4286

CN — — — 00A-4254-B0 00B-4254-B0 00D-4254-B0 00F-4254-B0 AJ0-4304

Phenyl-Hexyl 00A-4256-A0 00B-4256-A0 — 00A-4256-B0 00B-4256-B0 00D-4256-B0 00F-4256-B0 AJ0-4350

NH2 — — 00F-4377-A0 00A-4377-B0 00B-4377-B0 00D-4377-B0 00F-4377-B0 AJ0-4301

for ID: 2.0-3.0 mm

3 µm Narrow Bore and Analytical Columns (mm) SecurityGuard™ CartridgesPhase 30 x 3.0 50 x 3.0 150 x 3.0 30 x 4.6 50 x 4.6 75 x 4.6 100 x 4.6 150 x 4.6 4 x 2.0 mm 4 x 3.0 mm

/10pk /10pk

Silica(2) — — 00F-4162-Y0 — 00B-4162-E0 — 00D-4162-E0 00F-4162-E0 AJ0-4347 AJ0-4348

C8(2) 00A-4248-Y0 00B-4248-Y0 00F-4248-Y0 00A-4248-E0 00B-4248-E0 00C-4248-E0 00D-4248-E0 00F-4248-E0 AJ0-4289 AJ0-4290

C18(2) 00A-4251-Y0 00B-4251-Y0 00F-4251-Y0 00A-4251-E0 00B-4251-E0 00C-4251-E0 00D-4251-E0 00F-4251-E0 AJ0-4286 AJ0-4287

CN — 00B-4254-Y0 00F-4254-Y0 00A-4254-E0 00B-4254-E0 00C-4254-E0 00D-4254-E0 00F-4254-E0 AJ0-4304 AJ0-4305

Phenyl-Hexyl — 00B-4256-Y0 00F-4256-Y0 00A-4256-E0 00B-4256-E0 00C-4256-E0 00D-4256-E0 00F-4256-E0 AJ0-4350 AJ0-4351

NH2 — 00B-4377-Y0 00F-4377-Y0 00A-4377-E0 00B-4377-E0 — 00D-4377-E0 00F-4377-E0 AJ0-4301 AJ0-4302

for ID: 2.0-3.0 mm for ID: 3.2-8.0 mm

5 µm Microbore and Minibore Columns (mm) SecurityGuard™ CartridgesPhase 50 x 1.0 150 x 1.0 250 x 1.0 30 x 2.0 50 x 2.0 150 x 2.0 250 x 2.0 4 x 2.0 mm SecurityGuard

Cartridges require universal holder

Part No.: KJO-4282

/10pk

Silica(2) — — — — 00B-4274-B0 00F-4274-B0 00G-4274-B0 AJ0-4347

C5 — — — 00A-4043-B0 00B-4043-B0 00F-4043-B0 — AJ0-4292

C8 — — — 00A-4040-B0 00B-4040-B0 00F-4040-B0 — AJ0-4289

C8(2) 00B-4249-A0 00F-4249-A0 00G-4249-A0 00A-4249-B0 00B-4249-B0 00F-4249-B0 00G-4249-B0 AJ0-4289

C18 — — — 00A-4041-B0 00B-4041-B0 00F-4041-B0 00G-4041-B0 AJ0-4286

C18(2) 00B-4252-A0 00F-4252-A0 00G-4252-A0 00A-4252-B0 00B-4252-B0 00F-4252-B0 00G-4252-B0 AJ0-4286

CN — — — 00A-4255-B0 00B-4255-B0 00F-4255-B0 00G-4255-B0 AJ0-4304

Phenyl-Hexyl 00B-4257-A0 00F-4257-A0 00G-4257-A0 00A-4257-B0 00B-4257-B0 00F-4257-B0 00G-4257-B0 AJ0-4350

NH2 — 00F-4378-A0 — 00A-4378-B0 00B-4378-B0 00F-4378-B0 00G-4378-B0 AJ0-4301

for ID: 2.0-3.0 mm

5 µm Narrow Bore and Analytical Columns (mm) SecurityGuard™ CartridgesPhase 30 x 3.0 50 x 3.0 150 x 3.0 250 x 3.0 30 x 4.6 50 x 4.6 75 x 4.6 4 x 2.0 mm 4 x 3.0 mm SecurityGuard

Cartridges require universal holder.

Part No.: KJO-4282

/10pk /10pk

C8 — — 00F-4040-Y0 00G-4040-Y0 00A-4040-E0 00B-4040-E0 00C-4040-E0 AJ0-4289 AJ0-4290

C8(2) — 00B-4249-Y0 00F-4249-Y0 00G-4249-Y0 00A-4249-E0 00B-4249-E0 00C-4249-E0 AJ0-4289 AJ0-4290

C18 — — 00F-4041-Y0 00G-4041-Y0 00A-4041-E0 00B-4041-E0 00C-4041-E0 AJ0-4286 AJ0-4287

C18(2) 00A-4252-Y0 00B-4252-Y0 00F-4252-Y0 00G-4252-Y0 00A-4252-E0 00B-4252-E0 00C-4252-E0 AJ0-4286 AJ0-4287

CN — 00B-4255-Y0 00F-4255-Y0 00G-4255-Y0 00A-4255-E0 00B-4255-E0 00C-4255-E0 AJ0-4304 AJ0-4305

Phenyl-Hexyl 00A-4257-Y0 00B-4257-Y0 00F-4257-Y0 00G-4257-Y0 00A-4257-E0 00B-4257-E0 00C-4257-E0 AJ0-4350 AJ0-4351

NH2 — 00B-4378-Y0 00F-4378-Y0 00G-4378-Y0 00A-4378-E0 00B-4378-E0 00C-4378-E0 AJ0-4301 AJ0-4302

SCX — — — — — 00B-4398-E0 — AJ0-4307 AJ0-4308

for ID: 2.0-3.0 mm for ID: 3.2-8.0 mm


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Luna®

Method Validation Document

over 25 individual Chemical & Physical

tests!

Luna®

OUR tOP SELLInG HPLC COLUMn

For bulk media pricing and availability, please contact the Phenomenex Prep

Team at [email protected]

15 µm Preparative and Pilot Scale Columns (mm)Column Guards

Phase 250 x 4.6 250 x 21.2 250 x 30 250 x 50 50 x 50

Silica(2) 00G-4271-E0 00G-4271-P0 00G-4271-U0 00G-4271-V0 03B-4271-V0

C8(2) 00G-4272-E0 00G-4272-P0 00G-4272-U0 00G-4272-V0 03B-4272-V0

C18(2) 00G-4273-E0 00G-4273-P0 00G-4273-U0 00G-4273-V0 03B-4273-V0

Phenyl-Hexyl 00G-4286-E0 00G-4286-P0 00G-4286-U0 00G-4286-V0 03B-4286-V0


5 µm Analytical, Semi-Prep and Axia™ Packed Preparative Columns (mm) SecurityGuard™ Cartridges (mm)Phase 100 x 4.6 150 x 4.6 250 x 4.6 250 x 10 250 x 15 250 x 21.2 250 x 30 4 x 3.0* 10 x 10‡

/10pk /3pk

Silica(2) 00D-4274-E0 00F-4274-E0 00G-4274-E0 00G-4274-N0 — 00G-4274-P0-AX 00G-4274-U0-AX AJ0-4348 AJ0-7223

C5 00D-4043-E0 00F-4043-E0 00G-4043-E0 — — — — AJ0-4293 AJO-7372

C8 00D-4040-E0 00F-4040-E0 00G-4040-E0 00G-4040-N0 — — — AJ0-4290 AJ0-7222

C8(2) 00D-4249-E0 00F-4249-E0 00G-4249-E0 00G-4249-N0 00G-4249-AK 00G-4249-P0-AX 00G-4249-UO-AX AJ0-4290 AJ0-7222

C18 00D-4041-E0 00F-4041-E0 00G-4041-E0 00G-4041-N0 — — — AJ0-4287 AJ0-7221

C18(2) 00D-4252-E0 00F-4252-E0 00G-4252-E0 00G-4252-N0 00G-4252-AK 00G-4252-P0-AX 00G-4252-U0-AX AJ0-4287 AJ0-7221

CN 00D-4255-E0 00F-4255-E0 00G-4255-E0 00G-4255-N0 — 00G-4255-P0-AX — AJ0-4305 AJ0-7313

Phenyl-Hexyl 00D-4257-E0 00F-4257-E0 00G-4257-E0 00G-4257-N0 — 00G-4257-P0-AX 00G-4257-U0-AX AJ0-4351 AJ0-7314

NH2 00D-4378-E0 00F-4378-E0 00G-4378-E0 00G-4378-N0 — 00G-4378-P0-AX 00G-4378-U0-AX AJ0-4302 AJ0-7364

SCX 00D-4398-E0 00F-4398-E0 00G-4398-E0 00G-4398-N0 — 00G-4398-P0-AX — AJ0-4308 AJ0-7369

for ID: 3.2-8.0 mm for ID: 9-16 mm

10 µm Semi-Prep and Axia™ Packed Preparative Columns (mm) SecurityGuard™ Cartridges (mm)Phase 250 x 4.6 250 x 10 250 x 15 250 x 21.2 250 x 30 250 x 50 10 x 10‡ 15 x 21.2** 15 x 30.0♦

/3pk /ea /ea

Silica(2) 00G-4091-E0 00G-4091-N0 — 00G-4091-P0-AX 00G-4091-U0-AX — AJO-7223 AJ0-7229 AJ0-8312

C5 00G-4092-E0 00G-4092-N0 — 00G-4092-P0-AX — — AJO-7372 — —

C8 00G-4093-E0 00G-4093-N0 — 00G-4093-P0-AX — — AJO-7222 AJ0-7840 AJ0-8302

C8(2) 00G-4250-E0 00G-4250-N0 — 00G-4250-P0-AX 00G-4250-U0-AX 00G-4250-V0-AX AJO-7222 AJO-7840 AJ0-8302

C18 00G-4094-E0 00G-4094-N0 — 00G-4094-P0-AX — — AJO-7221 AJ0-7839 AJ0-8301

C18(2) 00G-4253-E0 00G-4253-N0 00G-4253-AK 00G-4253-P0-AX 00G-4253-U0-AX 00G-4253-V0-AX AJO-7221 AJO-7839 AJ0-8301

CN 00G-4300-E0 00G-4300-N0 — 00G-4300-P0-AX — — AJO-7313 AJO-8220 AJ0-8311

Phenyl-Hexyl 00G-4285-E0 00G-4285-N0 — 00G-4285-P0-AX 00G-4285-U0-AX — AJ0-7314 AJO-7841 AJ0-8303

NH2 00G-4379-E0 00G-4379-N0 — 00G-4379-P0-AX — — AJ0-7364 AJO-8162 AJ0-8309

SCX 00G-4401-E0 00G-4401-N0 — 00G-4401-P0-AX — — AJ0-7369 — —

for ID: 9-16 mm 18-29 mm 30-49 mm

For Luna HILIC, see p. 24For Luna PFP(2), see p. 25

*SecurityGuard™ Analytical Cartridges require holder, Part No.: KJ0-4282‡SemiPrep SecurityGuard™ Cartridges require holder, Part No.: AJ0-7220



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SecurityGuard™ Cartridges require universal holder Part No.: KJ0-4282

HPLC - SMALL MOLECULE

Luna® HILIC • Retains polar compounds • Elute in high organic mobile phase for optimized mass spectrometry results • Reproducible performance

0.50 1.0 1.5 2.0

1

1

2

2

3

3

2.5 3.0 3.5 4.0 4.5 min0

1000

2000

3000

4000

5000

6000

7000

0.50 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 min0

1000

2000

3000

4000

5000

6000

7000

Ap

p ID

163

34

Ap

p ID

163

44

0.50 1.0 1.5 2.0

1

1

2

2

3

3

2.5 3.0 3.5 4.0 4.5 min0

1000

2000

3000

4000

5000

6000

7000

0.50 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 min0

1000

2000

3000

4000

5000

6000

7000

Ap

p ID

163

34

Ap

p ID

163

44

Column: Luna 5 µm HILICDimensions: 100 x 2.0 mmMobile Phase:

A: 90:10 Acetonitrile / 100 mM NH Formate, pH 3.2B: 50:50 Acetonitrile / 20 mM NH Formate, pH 3.2

Gradient: 100:0 A/B for 1 min100:0 A/B to 0:100 A/B in 2 min 0:100 A/B for 1.5 min100:0 A/B for 5 min


Detection: MS

Sample: 1. Morphine-3-b-Glucuronide 2. Normorphine 3. Morphine

Column: Gemini 5 µm C18Dimensions: 100 x 2.0 mmMobile Phase:

A: Water with 0.1 % Formic acidB: Acetonitrile with 0.1 % Formic acid

Gradient: 97:3 A/B for 1 min97:3 A/B to 70:30 A/B in 2 min97:3 A/B for 2.49 min

Reversed Phase LC

HILIC

HILIC Mode LC

O

C

O

O

N

CH3

OH

OH

HO

HO O

OH

Morphine-3-b-Glucuronide

O

NH

HO

HO

H

Normorphine

O

NCH3

H

HO

HO

Morphine

Reversal of Elution Order. Longer Retention for Glucuronide!

Interested in exploring HILIC chromatography? Request the HILIC Method Screening Approach paper from your authorized Phenomenex distributor.

Polar Retention & Complimentary Selectivity to Reversed Phase

Luna 3 µm HILIC SecurityGuard™ Cartridges (mm)

Part No. Dimensions (mm) /10pk

00B-4449-B0 50 x 2.0 AJ0-8328

00D-4449-B0 100 x 2.0 AJ0-8328

00F-4449-B0 150 x 2.0 AJ0-8328

00B-4449-Y0 50 x 3.0 AJ0-8328

00D-4449-Y0 100 x 3.0 AJ0-8328

00F-4449-Y0 150 x 3.0 AJ0-8328

00D-4449-E0 100 x 4.6 AJ0-8329

00F-4449-E0 150 x 4.6 AJ0-8329

Luna 5 µm HILICPart No. Dimensions (mm) /10pk

00F-4450-Y0 150 x 3.0 AJ0-8328

00D-4450-E0 100 x 4.6 AJ0-8329

00F-4450-E0 150 x 4.6 AJ0-8329

00G-4450-E0 250 x 4.6 AJ0-8329



Detection: MS

Sample: 1. Morphine-3-b-Glucuronide 2. Normorphine 3. Morphine


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HPLC - SMALL MOLECULE

Luna® PFP(2) • Multiple retention mechanisms for alternate selectivity • Orthogonal selectivity (to traditional C18 phases) for more success in your method screening • Unique polar interactions for trace impurity identification

Positional Isomers of Halogenated Phenols on Luna 3 µm PFP(2)

Column: Luna 3 µm PFP(2) Dimension: 150 x 4.6 mm Part No.: 00F-4447-E0 Mobile Phase: A: 0.1 % Formic acid in Water B: 0.1 % Formic acid in Acetonitrile Gradient: A/B (60:40) to (50:50) in 10 min Flow Rate: 1 mL/min Temperature: 22 °C Detection: UV @ 254 nm Sample: 1. 2,3-Dimethylphenol 6. 2,5-Dichlorophenol 2. 2,5-Dimethylphenol 7. 2,6-Dichlorophenol 3. 2,6-Dimethylphenol 8. 3,4-Dichlorophenol 4. 3,4-Dimethylphenol 9. 3,5-Dichlorophenol 5. 3,5-Dimethylphenol 10. 2,4-Dibromophenol

12

34

5

6

7

8

9

10

0 2 4 6 8 10 12 14 min

baseline ! separation

App

ID: 1

6296

16297

0 2 4 6 8 10 12 14 min

12

34

5

67

8

9 & 10

Positional Isomers of Halogenated Phenols on Luna 3 µm C18(2)

Column: Luna 3 µm C18(2) Dimension: 150 x 4.6 mm ID Part No.: 00F-4251-E0 Mobile Phase: A: 0.1% Formic acid in Water B: 0.1% Formic acid in Acetonitrile Gradient: A/B (60:40) to (50:50) in 10 min Flow Rate: 1 mL/min Temperature: 22 °C Detection: UV @ 254 nm Sample: 1. 2,3-Dimethylphenol 6. 2,5-Dichlorophenol 2. 2,5-Dimethylphenol 7. 2,6-Dichlorophenol 3. 2,6-Dimethylphenol 8. 3,4-Dichlorophenol 4. 3,4-Dimethylphenol 9. 3,5-Dichlorophenol 5. 3,5-Dimethylphenol 10. 2,4-Dibromophenol

App

ID: 1

6297

Luna 3 µm PFP(2) SecurityGuard™ Cartridges (mm)


00B-4447-B0 50 x 2.0 AJ0-8326

00F-4447-B0 150 x 2.0 AJ0-8326

00B-4447-Y0 50 x 3.0 AJ0-8326

00F-4447-Y0 150 x 3.0 AJ0-8326

00B-4447-E0 50 x 4.6 AJ0-8327

00D-4447-E0 100 x 4.6 AJ0-8327

00F-4447-E0 150 x 4.6 AJ0-8327

00G-4447-E0 250 x 4.6 AJ0-8327



00B-4448-B0 50 x 2.0 AJ0-8326

00F-4448-B0 150 x 2.0 AJ0-8326

00B-4448-Y0 50 x 3.0 AJ0-8326

00F-4448-Y0 150 x 3.0 AJ0-8326

00B-4448-E0 50 x 4.6 AJ0-8327

! co-elution



00B-4448-P0-AX 50 x 21.2 AJ0-8377**

00D-4448-P0-AX 100 x 21.2 AJ0-8377**

00F-4448-P0-AX 150 x 21.2 AJ0-8377**

00G-4448-P0-AX 250 x 21.2 AJ0-8377**

00B-4448-U0-AX 50 x 30 AJ0-8378♦

00D-4448-U0-AX 100 x 30 AJ0-8378♦

00F-4448-U0-AX 150 x 30 AJ0-8378♦

00G-4448-U0-AX 250 x 30 AJ0-8378♦

for ID: 18.0 - 49.0 mm

PFP(2)

C18(2)

ORDERING INFORMATION SecurityGuard™ Cartridges require universal holder Part No.: KJ0-4282



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CHIRAL StAtIOnARY PHASES

LUX™ Cellulose-2 • Unique enantioselectivity • Excellent for chiral screening • Complimentary selectivity with Lux™ Cellulose-1 • Pressure rated to 300 bar

Phenyl rings contribute to π- π interactions.

Lux™ Cellulose-2 uses the chiral selector cellulose tris(3-chloro-4-methylphenylcarbamate)

Electronegative atoms, such as oxygen, nitrogen, and chlorine are responsible for hydrogen bonding.

Chlorine atoms have strong electronegativity, contributing to dipole interactions.

Cellulose backbones form linear chains, creating various grooves and cavities which provide steric and inclusion interactions.

17349

0 2

2

1

4 6 8 min

0

200

400

mAU

17350

0 2

2

1

4 6 8 min

0

200

400

mAU

Mephenytoin

N

HN

O

CH3

H 3C

O

Lux™ Cellulose-2a = 1.83

*CHIRALCEL® OD-H®

a = 1.19

Ap

p ID

173

49A

pp

ID 1

7350

Column: Lux™ 5 µm Cellulose-2Dimensions: 250 x 4.6 mm ID

Part No.: 00G-4457-E0Mobile Phase: 0.1 % Diethylamine in Hexane /

0.1 % Diethylamine in Isopropanol (80:20)Flow Rate: 1 mL/minDetection: UV @ 220 nm

Temperature: AmbientSample: Mephenytoin

Column: *CHIRALCEL® 5 µm OD-H®

Dimensions: 250 x 4.6 mm IDMobile Phase: 0.1 % Diethylamine in Hexane /


Temperature: AmbientSample: Mephenytoin

*CHIRALCEL and OD-H are registered trademarks of DAICEL Chemical Industries, Ltd. Comparative separations may not be representative of all applications.


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CHIRAL StAtIOnARY PHASES

LUX™ Cellulose-1 • Cost-effective replacement to *CHIRALCEL® OD-H®

• Broad enantioselectivity • Complimentary selectivity with Lux™ Cellulose-2 • Pressure rated to 300 bar

Lux™ Cellulose-1 uses the chiral selector cellulose tris(3,5-dimethylphenylcarbamate)

Phenyl rings contribute to π- π interactions.

Electronegative atoms, such as oxygen and nitrogen, are responsible for hydrogen bonding.

Cellulose backbones form linear chains, creating various grooves and cavities which provide steric interactions.

Homatropine

0 1 2 3 4 5 min

1

2

0

200

400

600

800

mAU

17378

0 1 2 3 4 5 min

1

2

0

200

400

600

800

mAU

17379

Lux™ Cellulose-1a = 1.82

*CHIRALCEL® OD-H®

a = 1.67

Ap

p ID

173

78A

pp

ID 1

7379

N+

O

O

O H

CH3

CH3

Column: Lux™ 5 µm Cellulose-1Dimensions: 250 x 4.6 mm ID

Part No.: 00G-4459-E0Mobile Phase: 0.1 % Diethylamine in Methanol /


Temperature: AmbientSample: Homatropine

Column: *CHIRALCEL® 5 µm OD-H®

Dimensions: 250 x 4.6 mm IDMobile Phase: 0.1 % Diethylamine in Methanol /


Temperature: AmbientSample: Homatropine

3 μm Analytical Columns (mm) SecurityGuard™ Cartridges (mm)150 x 2.0 100 x 4.6 150 x 4.6 250 x 4.6 4 x 2.0* 4 x 3.0*

Phases /10pk /10pk

Lux Cellulose-1 00F-4458-B0 00D-4458-E0 00F-4458-E0 00G-4458-E0 AJ0-8402 AJ0-8403

Lux Cellulose-2 00F-4456-B0 00D-4456-E0 00F-4456-E0 00G-4456-E0 AJ0-8398 AJ0-8366

for ID: 2.0–3.0 mm 3.2–8.0 mm

5 μm Analytical Columns (mm) SecurityGuard™ Cartridges (mm)100 x 4.6 150 x 4.6 250 x 4.6 4 x 3.0*

Phases /10pk

Lux Cellulose-1 00D-4459-E0 00F-4459-E0 00G-4459-E0 AJ0-8403

Lux Cellulose-2 00D-4457-E0 00F-4457-E0 00G-4457-E0 AJ0-8366

for ID: 3.2–8.0 mm

5 μm Axia™ Packed Preparative Columns (mm) SecurityGuard™ Cartridges (mm)150 x 21.2 250 x 21.2 250 x 30 250 x 50 15 x 21.2** 15 x 30.0+

Phases /ea /ea

Lux Cellulose-1 00F-4459-P0-AX 00G-4459-P0-AX 00G-4459-U0-AX 00G-4459-V0-AX AJ0-8405 AJ0-8406

Lux Cellulose-2 00F-4457-P0-AX 00G-4457-P0-AX 00G-4457-U0-AX 00G-4457-V0-AX AJ0-8400 AJ0-8401

for ID: 18–29 mm 30–49 mm

Column Performance Check StandardPart No. Description Unit Price

AL0-8412 Chiral Test Mix No. 5 (Lux) ea


*CHIRALCEL and OD-H are registered trademarks of DAICEL Chemical Industries, Ltd. Comparative separations may not be representative of all applications.

*SecurityGuard Analytical Cartridges require holder, Part No. : KJ0-4282**PREP SecurityGuard Cartridges require holder, Part No. : AJ0-8223+PREP SecurityGuard Cartridges require holder, Part No. : AJ0-8277


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Now Available in 150 & 250 mm Lengths - 21.2, 30, and 50 mm ID’s

tHE FUtURE OF PREPARAtIVE CHROMAtOGRAPHY

Compare LifetimeAn Axial Compression System in a High-Throughput Preparative Column!

Axia Packed Leading Competitive Preparative Column

Luna® 5 µm C18(2) Axia Packed Waters® XTerra® 5 µm Prep MS C18 OBD™

First Run 800th run First Run 120th run** Six columns tested. Best lifetime chosen for comparison.

Axia™

• Award winning Prep LC column technology • Significantly longer lifetime with patent-pending technology • Improved column-to-column reproducibility • Recover higher compound purity with analytical-like efficiency • Axia columns packed with high surface area media ideal for preparative chromatography

3

0

500

2 4 min

mAu

3

3

0

500

2

21 321

4 min

mAu

vs. Conditions: (same for both columns except where noted) Columns: Luna 5 µm C18(2) Axia Packed Waters XTerra 5 µm Prep MS C18 OBD Dimension: 50 x 21.2 mm (Luna) 50 x 19 mm (XTerra) Mobile Phase: A: 0.5 % TFA in Water B: 0.5 % TFA in Acetonitrile Gradient: Linear 95:5 (A/B) to 5:95 (A/B) over 7 min, hold 3 min Injection Volume: 500 μL Flow Rate: 30 mL/min (Luna) 24 mL/min (XTerra)‡

Temperature: Ambient Detection: UV @ 254 nm Sample: 1. Triprolidine 1.6 mg 2. Methacycline 16 mg 3. Amitriptyline 5.25 mg ‡ Same linear velocity

Reproducible Column-to-Column Efficiency

Reproducible Column-to-Column Peak Asymmetry

Average Efficiency (N) with Synergi 4 µm Hydro-RP 100 x 21.2 mm

Average Peak Asymmetry with Gemini 5 µm C18 50 x 21.2 mm

27 % Improved Avg. Efficiency 13 % Improved Avg. Peak Shape

Expect an Excellent Axia Column. Every Time. Consistent Quality. Column-to-Column. Batch-to-Batch.

: Visit www.axiaprep.com to view the Award winning technology in action.Conventional

Slurry PackingAxia Packed

Hydraulic Piston Compression Packing

ConventionalSlurry Packing

Axia PackedHydraulic Piston

Compression Packing

Effic

ienc

y (N

)

Peak

Asy

mm

etry

1

1.1

1.2

1.3

1.4

40000

60000

80000

100000

120000

11.8

2.5

5.2

9.2

% RSD % RSDAxia Hydraulic Piston Compression (Patent Pending)

With the Axia—hydraulic piston compression technology—the piston compresses the bed and pressure is never released (no chance of media loss). The optimum slurry amount and packing pressure are automated for reproducible packing control. And in the final product, the piston remains in place, yielding an excellent column with extended lifetime and consistent peak shape. Every time.


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tHE FUtURE OF PREPARAtIVE CHROMAtOGRAPHY


Conditions: (same for both columns except where noted) Columns: Luna 5 µm C18(2) Axia Packed Waters XTerra 5 µm Prep MS C18 OBD Dimension: 50 x 21.2 mm (Luna) 50 x 19 mm (XTerra) Mobile Phase: A: 0.5 % TFA in Water B: 0.5 % TFA in Acetonitrile Gradient: Linear 95:5 (A/B) to 5:95 (A/B) over 7 min, hold 3 min Injection Volume: 500 μL Flow Rate: 30 mL/min (Luna) 24 mL/min (XTerra)‡

Temperature: Ambient Detection: UV @ 254 nm Sample: 1. Triprolidine 1.6 mg 2. Methacycline 16 mg 3. Amitriptyline 5.25 mg ‡ Same linear velocity

50 x 21.2 mm 100 x 21.2 mm 150 x 21.2 mm 250 x 21.2 mm 50 x 30 mm 100 x 30 mmGemini 5 µm C18 00B-4435-P0-AX 00D-4435-P0-AX 00F-4435-P0-AX 00G-4435-P0-AX 00B-4435-U0-AX 00D-4435-U0-AXGemini 10 µm C18 — — — 00G-4436-P0-AX — —Gemini 5 µm C6-Phenyl 00B-4444-P0-AX 00D-4444-P0-AX 00F-4444-P0-AX 00G-4444-P0-AX 00B-4444-U0-AX 00D-4444-U0-AXGemini-NX 5 µm C18 00B-4454-P0-AX 00D-4454-P0-AX 00F-4454-P0-AX 00G-4454-P0-AX 00B-4454-U0-AX 00D-4454-U0-AXGemini-NX 10 µm C18 — — — 00G-4455-P0-AX — —

Luna 5 µm C18(2) 00B-4252-P0-AX 00D-4252-P0-AX 00F-4252-P0-AX 00G-4252-P0-AX 00B-4252-U0-AX 00D-4252-U0-AXLuna 10 µm C18(2) — — — 00G-4253-P0-AX — —Luna 5 µm C8(2) 00B-4249-P0-AX 00D-4249-P0-AX 00F-4249-P0-AX 00G-4249-P0-AX 00B-4249-U0-AX 00D-4249-U0-AXLuna 10 µm C8(2) — — — 00G-4250-P0-AX — —Luna 5 µm CN 00B-4255-P0-AX — 00F-4255-P0-AX 00G-4255-P0-AX — 00D-4255-U0-AXLuna 5 µm NH2 — 00D-4378-P0-AX 00F-4378-P0-AX 00G-4378-P0-AX — 00D-4378-U0-AXLuna 5 µm PFP(2) 00B-4448-P0-AX 00D-4448-P0-AX 00F-4448-P0-AX 00G-4448-P0-AX 00B-4448-U0-AX 00D-4448-U0-AXLuna 5 µm Phenyl-Hexyl 00B-4257-P0-AX 00D-4257-P0-AX 00F-4257-P0-AX 00G-4257-P0-AX 00B-4257-U0-AX 00D-4257-U0-AXLuna 10 µm Phenyl-Hexyl — — — 00G-4285-P0-AX — —Luna 5 µm Silica(2) 00B-4274-P0-AX 00D-4274-P0-AX 00F-4274-P0-AX 00G-4274-P0-AX 00B-4274-U0-AX 00D-4274-U0-AXLuna 10 µm Silica(2) — — — 00G-4091-P0-AX — —

Lux 5 µm Cellulose-1 — 00D-4459-P0-AX — 00G-4459-P0-AX — —Lux 5 µm Cellulose-2 — 00D-4457-P0-AX — 00G-4457-P0-AX — —

Synergi 4 µm Max-RP 00B-4337-P0-AX 00D-4337-P0-AX 00F-4337-P0-AX 00G-4337-P0-AX 00B-4337-U0-AX 00D-4337-U0-AXSynergi 10 µm Max-RP — — — 00G-4350-P0-AX — —Synergi 4 µm Hydro-RP 00B-4375-P0-AX 00D-4375-P0-AX 00F-4375-P0-AX 00G-4375-P0-AX 00B-4375-U0-AX 00D-4375-U0-AXSynergi 10 µm Hydro-RP — — — 00G-4376-P0-AX — —Synergi 4 µm Polar-RP 00B-4336-P0-AX 00D-4336-P0-AX 00F-4336-P0-AX 00G-4336-P0-AX 00B-4336-U0-AX 00D-4336-U0-AXSynergi 10 µm Polar-RP — — — 00G-4351-P0-AX — —Synergi 4 µm Fusion-RP 00B-4424-P0-AX 00D-4424-P0-AX — — — —

150 x 30 mm 250 x 30 mm 50 x 50 mm 100 x 50 mm 150 x 50 mm 250 x 50 mm 21.2* 30.0**Gemini 5 µm C18 00F-4435-U0-AX 00G-4435-U0-AX 00B-4435-V0-AX — — — AJ0-7839 AJ0-8301Gemini 10 µm C18 — 00G-4436-U0-AX — 00D-4436-V0-AX 00F-4436-V0-AX 00G-4436-V0-AX AJ0-7839 AJ0-8301Gemini 5 µm C6-Phenyl 00F-4444-U0-AX 00G-4444-U0-AX — — — — AJ0-7841 AJ0-8303Gemini-NX 5 µm C18 00F-4454-U0-AX 00G-4454-U0-AX 00B-4454-V0-AX — — — AJ0-8370 AJ0-8371Gemini-NX 10 µm C18 — 00G-4455-U0-AX — 00D-4455-V0-AX 00F-4455-V0-AX 00G-4455-V0-AX AJ0-8370 AJ0-8371

Luna 5 µm C18(2) 00F-4252-U0-AX 00G-4252-U0-AX 00B-4252-V0-AX — — — AJ0-7839 AJ0-8301Luna 10 µm C18(2) — 00G-4253-U0-AX — 00D-4253-V0-AX — 00G-4253-V0-AX AJ0-7839 AJ0-8301Luna 5 µm C8(2) 00F-4249-U0-AX 00G-4249-U0-AX 00B-4249-V0-AX — — — AJ0-7840 AJ0-8302Luna 10 µm C8(2) — 00G-4250-U0-AX — — — 00G-4250-V0-AX AJ0-7840 AJ0-8302Luna 5 µm CN 00F-4255-U0-AX 00G-4255-U0-AX — — — — AJ0-7840 AJ0-8302Luna 5 µm NH2 00F-4378-U0-AX 00G-4378-U0-AX — — — — AJ0-8162 AJ0-8309Luna 5 µm PFP(2) 00F-4448-U0-AX 00G-4448-U0-AX — — — — AJ0-8162 AJ0-8309Luna 5 µm Phenyl-Hexyl 00F-4257-U0-AX 00G-4257-U0-AX — — — — AJ0-7841 AJ0-8303Luna 5 µm Silica(2) 00F-4274-U0-AX 00G-4274-U0-AX — — — — AJ0-7229 AJ0-8312Luna 10 µm Silica(2) — 00G-4091-U0-AX — — — 00G-4091-V0-AX AJ0-7229 AJ0-8312

Lux 5 µm Cellulose-1 — 00G-4459-U0-AX — — — 00G-4459-V0-AX AJ0-8405 AJ0-8406Lux 5 µm Cellulose-2 — 00G-4457-U0-AX — — — 00G-4457-V0-AX AJ0-8400 AJ0-8401

*PREP SecurityGuard™ Cartridges require holder, Part No.: AJO-8223**PREP SecurityGuard™ Cartridges require holder, Part No.: AJO-8277


21.2* 30.0**AJ0-7839 AJ0-8301AJ0-7839 AJ0-8301AJ0-7841 AJ0-8303AJ0-8370 AJ0-8371AJ0-8370 AJ0-8371

AJ0-7839 AJ0-8301AJ0-7839 AJ0-8301AJ0-7840 AJ0-8302AJ0-7840 AJ0-8302AJ0-7840 AJ0-8302AJ0-8162 AJ0-8309AJ0-8162 AJ0-8309AJ0-7841 AJ0-8303AJ0-7841 AJ0-8303AJ0-7229 AJ0-8312AJ0-7229 AJ0-8312

AJ0-8405 AJ0-8406AJ0-8400 AJ0-8401

AJ0-7842 AJ0-8304AJ0-7842 AJ0-8304AJ0-7843 AJ0-8305AJ0-7843 AJ0-8305AJ0-7845 AJ0-8307AJ0-7845 AJ0-8307

AJ0-7844 AJ0-8306

SecurityGuard™ Cartridges (mm)

Additional Axia dimensions available-please contact your authorized Phenomenex distributor.


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Clarity® BioSeparations • Extensive product portfolio for synthetic DNA/RNA purification & characterization • Purify several types of synthetic oligonucleotides, including DNA, RNA/RNAi, longmers, dye-labeled, & modified • Recoveries and purities ranging from 70 – 95 % depending on solution selected • High-throughput, high purity solution available with R&D 100 Award winning Clarity QSP™

YOUR PARtnER In OLIGOnUCLEOtIDE PURIFICAtIOn

Clarity Desalting • Quick, simple removal of salt and excess reagent • Reversed phase desalting method results in low conductivity • 80 % typical recovery of target synthetic DNA/RNA sequence • Economical & disposable format

Clarity Desalting TubesPhase 200 mg/3 mL* 500 mg/3 mL**

50/box 50/box

C18 8B-S041-FBJ 8B-S041-HBJ

* For 200 µmole synthesis** For 1 µmole synthesis

Clarity QSP Clarity Oligo-RP Clarity Oligo-WAX Clarity Desalting

Format(s) 50 mg/ 96-well plate

150 mg/ 3 mL cartridges 5 g/ 60 mL cartridges

Analytical, semi-prep, & preparative HPLC columns

Analytical, semi-prep, & preparative HPLC columns

200 mg/ 3 mL tubes 500 mg/ 3 mL tubes

Equipment Required VVacuum manifold or automated liquid handling system HPLC system HPLC OR FPLC systems Vacuum manifold

Purification Time ~8 minutes per cartridge

~45 minutes per well platee15 – 30 minutes 3 20 – 60 minutes 3 15 minutes

Oligo Length ≤ 100 nt ≤ 60 nt ≤ 80 nt ≤ 60 nt

Synthesis Scale Load 1 nmole – 50 µmole 1 nmole – 50 µmole 1 nmole – 50 µmole ≤ 1 µmole

Typical Purity 90 – 95 % 1 90 – 95 % 4 ≥ 90 % ~70 % 4

Typical Recovery Yield ~90 % 2 ~70 % ≥ 90 % ~80 %

1. Purity value based on ion-exchange chromatography and capillary electrophoresis 2. OD260 used for quantitation 3. Time range is dependent on dimension of HPLC column and flow rate4. Purity value based on reversed phase chromatography

30



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Clarity® QSP™ Cartridges and Well Plates • High-throughput, high purity cartridge & 96-well plate solutions for synthetic RNA & DNA • 90 % typical purities and yields • Simple 3-step, economical process delivers highly purified DNA/RNA in minutes • Formats easily amenable to parallel processing equipment • No toxic ion pairing reagents to interfere with downstream applications


Detritylated Failure SequencesTrityl GroupFull Length Target Oligo

Full Length Trityl - On OligoImpurityN-1 Sequence

STEP 3

Elution of Target Oligo

Organic M

ixture

Elute target oligopH buffered solutions used to

maintain safe pH for oligo;select elution buffer based on

downstream requirements

STEP 2

Detritylate

DC

A

DetritylateLess than 2 %

depurination observed.A faint orange band

will appear at top halfof cartridge indicating

DMT retention

STEP 1

Loading of Crude Oligo

Load crude oligo cocktailAll trityl-off impurities flowdirectly through; no wash

required

Part No. Description Unit PriceFormats

8E-S102-DGB Clarity QSP Well Plate 50 mg/well 1/box8B-S102-SBJ Clarity QSP Cartridge 150 mg/3 mL 50/box8B-S042-LFF Clarity QSP Cartridge 5 g/60 mL 16/box

Buffers*

AL0-8279 Clarity QSP DNA Loading Buffer 100 mL eaAL0-8280 Clarity QSP DNA Loading Buffer 1 L eaAL0-8281 Clarity QSP RNA-TDBMS Loading Buffer 100 mL eaAL0-8282 Clarity QSP RNA-TDBMS Loading Buffer 1 L eaAH0-7858 Clarity Nuclease Free Water 1 L ea

Accessories

AH0-6024 24-Position Vacuum Manifold Complete Set eaAH0-7284 96-Well Plate Manifold Acrylic eaAH0-7194 96 Square Well Collection Plate, Conical V-bottom 2 mL/well (Polypropylene) 50/pkAH0-7408 Solvent Waste Reservoir Tray for Well Plate Manifolds 25/pkAH0-7195 96-Well Pierceable Sealing Mat Square Well 50/pk


* RNA-TOM loading buffer available upon request.

2008 R&D 100 Award Recipient


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Material Characteristics

Clarity® Oligo-RP™

• Easily purify N-1 failure sequences from target oligo with > 90 % purities • MS-friendly protocol for characterization of synthetic DNA and RNA • Analytical columns through bulk material available to support various loading capacities • Excellent column for reversed phase HPLC quality control testing


Excellent selectivity characteristics of Clarity oligo-rP allow baseline separation of failure N-1 sequences from target N sequences.

min 0 5 10 15 20 25 30 35 40

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30

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50

21 nt20 nt

15935

App

ID 1

5935

Column: Clarity 3 μm Oligo-RP C18Dimensions: 50 x 4.6 mm

Part No.: 00B-4441-E0Mobile Phase: A: 10 mM TPAC

B: MethanolGradient: A/B (75:25) to A/B (55:45) in 80 min (50

min run time)Flow Rate: 1 mL/minDetection: UV @ 260 nm

Sample: 1. 20 nt RNA with sequence CUGUAAUCUCUUGUCUATT (2.5 μg) 2. 21 nt RNA with sequence UCUGUAAUCUCUUGUCUATT (2.5 μg)

Clarity® Oligo-RP™

Particle Support TWIN™ (silica organic composite)Bonded Phase C18Particle Size 3, 5, and 10 µmPore Size 110 ÅSurface Area 375 m2/gpH Stability 1.0 – 12.0 pH Effective Range 1.0 – 12.0

RNA Purification of Failure N-1 Sequence from Target N Sequence

3 µm Columns (mm)Phases 50 x 1.0 100 x 1.0 50 x 2.0 100 x 2.0 150 x 2.0Oligo-RP 00B-4441-A0 00D-4441-A0 00B-4441-B0 00D-4441-B0 00F-4441-B0

Phases 50 x 4.6 100 x 4.6 150 x 4.6 50 x 10.0Oligo-RP 00B-4441-E0 00D-4441-E0 00F-4441-E0 00B-4441-N0

5 µm Columns (mm)Phases 50 x 1.0 150 x 2.0 50 x 4.6 150 x 4.6 250 x 4.6Oligo-RP 00B-4442-A0 00F-4442-B0 00B-4442-E0 00F-4442-E0 00G-4442-E0

Phases 50 x 10.0 100 x 10.0 250 x 10.0 250 x 21.2 250 x 30.0

Oligo-RP 00B-4442-N0 00D-4442-N0 00G-4442-N0 00G-4442-P0-AX 00G-4442-U0

10 µm Columns (mm)Phases 150 x 4.6 150 x 10.0 150 x 30.0 150 x 50.0Oligo-RP 00F-4445-E0 00F-4445-N0 00F-4445-U0 00F-4445-V0

Phases 250 x 10.0 250 x 21.2 250 x 50.0

Oligo-RP 00G-4445-N0 00G-4445-P0-AX 00G-4445-V0

*SecurityGuard Analytical Cartidges require holder, Part No.: KJ0-4282 ‡SecurityGuard Analytical Cartidges require holder, Part No.: AJ0-7220 **SecurityGuard Analytical Cartidges require holder, Part No.: AJ0-8223

AJ0-8134 SecurityGuard™ Cartridges Clarity Oligo-RP 4 x 2.0 mm* 10/Pk 2.0 - 3.0 mmAJ0-8135 SecurityGuard Cartridges Clarity Oligo-RP 4 x 3.0 mm* 10/Pk 3.2 - 8.0 mmAJ0-8136 SecurityGuard Semi-Prep Cartridges Clarity Oligo-RP 10 x 10 mm‡ 3/Pk 9 - 16 mmAJ0-8210 SecurityGuard PREP Cartridge Clarity Oligo-RP 15 x 21.2 mm ID** Ea 18 - 29 mmAJ0-8310 SecurityGuard PREP Cartridge Clarity Oligo-RP 15 x 30 mm ID** Ea 30 - 49 mm

Part No. Description Unit For ID

GUARD CARTRIDGE SYSTEM



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Clarity® Oligo-WAX™

• Higher loading capacity ion-exchange, silica material for prep/process purifications • Better fractionation of closely eluting compounds resulting in higher purity • Increase productivity by running at higher flow rates and pressures

Two purification runs were performed on each column with fractional QC being taken after each run. Passing fractions from the two purification runs were combined into one pooled lot for each column. That pooled lot was then divided equally and run through a Clarity desalting tube. Final OD’s and QC were taken after desalting, including ESI, CE, and conductivity. The purity and resolution of Clarity Oligo-WAX was considerably better than SOURCE 15Q. Though SOURCE had a slightly higher recovery of full length oligo, it was not a wide enough margin to offset the purity advantage.

GE™ Healthcare SOURCE™ 15Q 150 x 10 mm (self-packed) Clarity Oligo-WAX 10 µm 150 x 10 mm (pre-packed)

• Final Purity = 88.8 %, N-1 = 2.0 % • Final amount = 205.9 OD’s• Recovery of full-length product = 28.9 % • Conductivity = 200 µS/cm

• Final Purity = 95.1 %, N-1 = 1.3 % • Final amount = 188.9 OD’s• Recovery of full-length product = 28.4 % • Conductivity = 151 µS/cm

Data courtesy of a large, Iowa-based oligo manufacturer. Comparative separations may not be representative of all applications.

* SecurityGuard™ Analytical Cartridges require universal holder Part No.: KJ0-4282 ‡ Semi-prep SecurityGuard™ require universal holder Part No.: AJ0-7220 ** Prep SecirutyGuard™ require universal holder Part No.: AJ0-8223 u Prep SecirutyGuard™ require universal holder Part No.: AJ0-8277

CE Purity Analysis of Ion-Exchange Purification

Clarity Oligo-WAX™ HPLC Columns SecurityGuard™ Cartridges (mm)

10 µm Minibore, Analytical, Semi-Prep & Prep Columns (mm)

Phase 100 x 4.6 150 x 4.6 150 x 10.0 150 x 21.2 150 x 30.0 250 x 10.0 4 x 3.0* 10 x 10‡

/10 pk /3 pkOligo-WAX 00D-4451-E0 00F-4451-E0 00F-4451-N0 00F-4451-P0 00F-4451-U0 00G-4451-N0 AJ0-8324 AJ0-8325

for ID: 3.2-8.0 mm 9-16 mm



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RP-HPLC PROtEIn/PEPtIDE CHARACtERIZAtIOn AnD PURIFICAtIOn

Jupiter® 300 300 Å columns designed to analyze and purify intact proteins (> 10,000 MW)

• C18, C5, and C4 bonded phases • 1.5 – 10 pH stability for method ruggedness and easy protein removal • Direct scale-up to preparative and bulk materials

Jupiter® Proteo 90 Å columns engineered for increased peak capacity and resolution of peptide maps/peptide separations

(< 10,000 MW)

• Novel C12 bonded phase for excellent selectivity • Identify post-translational modifications • Capillary columns available for increased sensitivity

a-Chymotrypsin Tryptic Digest on Jupiter Proteo

Column: Jupiter 4 μm Proteo 90 ÅDimensions: 150 x 0.5 mmPart No.: 00F-4396-AFMobile Phase: A: 0.01 % TFA in Water B: 0.008 % TFA in Acetonitrile

Gradient: A/B (95:5) for 5 min then A/B (55:45) in 45 min Switch to A/B (95:5) in 1 sec then hold for 10 min Flow Rate: 15 µL/minTemperature: 40 °CDetection: UV @ 210 nmSample: a - Chymotrypsin tryptic digest

App ID 14336

0 10 20 30 40 min

for ID: 2.0-3.0 mm

3 μm, 4 μm & 5 μm microbore and minibore Columns (mm) SecurityGuard™ Cartridges (mm) 50 x 1.0 150 x 1.0 250 x 1.0 50 x 2.0 150 x 2.0 250 x 2.0 4 x 2.0*Phases /10pk5 μm C4 300 Å I 00B-4167-A0 I 00F-4167-A0 I 00G-4167-A0 I 00B-4167-B0 I 00F-4167-B0 I 00G-4167-B0 AJ0-43295 μm C5 300 Å 00B-4052-A0 00F-4052-A0 00G-4052-A0 00B-4052-B0 00F-4052-B0 00G-4052-B0 AJ0-43265 μm C18 300 Å 00B-4053-A0 00F-4053-A0 00G-4053-A0 00B-4053-B0 00F-4053-B0 00G-4053-B0 AJ0-43204 μm Proteo 90 Å 00B-4396-A0 00F-4396-A0 00G-4396-A0 00B-4396-B0 00F-4396-B0 00G-4396-B0 AJO-60733 μm C18 300 Å — — — 00B-4263-B0 00F-4263-B0 — AJO-4320

4 μm & 5 μm Capillary Columns (mm) 50 x 0.30 150 x 0.30 250 x 0.30 50 x 0.50 150 x 0.50 250 x 0.50 Phases

5 μm C4 300 Å I 00B-4167-AC I 00F-4167-AC I 00G-4167-AC I 00B-4167-AF I 00F-4167-AF I —5 μm C18 300 Å 00B-4053-AC 00F-4053-AC 00G-4053-AC 00B-4053-AF 00F-4053-AF 00G-4053-AF4 μm Proteo 90 Å 00B-4396-AC 00F-4396-AC 00G-4396-AC 00B-4396-AF 00F-4396-AF 00G-4396-AF


18-29 mmfor ID: 3.2-8.0 mm 9-16 mm

*SecurityGuard™ Analytical Cartridges require holder, Part No.: KJ0-4282

‡SemiPrep SecurityGuard™ Cartridges require holder, Part No.: AJ0-7220

**PREP SecurityGuard™ Cartridges require holder, Part No.: AJ0-8223

Large Proteins on Jupiter 300 C4Column: Jupiter 300 5 µm C4Dimensions: 150 x 4.6mmPart No.: 00F-4167-E0Mobile Phase: A) 0.1% TFA in Water B) 0.1% TFA in 90% AcetonitrileGradient: A/B (95:5) to A/B (20:80) in 20 minutesFlow Rate: 1 mL/minTemperature: 35 °CDetection: UV @ 280 nmInjection Volume: 20 µLSample: Large Proteins 1. Bovine Serum Albumin (66 kDa) 2. Glutamic Dehydrogenase (56 kDa) 3. Ovalbumin (44 kDa) 4. Phosphorylase b (97 kDa)

min0 2.5 5 7.5 10 12.5 15 17.5 20

mAU

0

10

20

30

40

50

60

70

801

2

3

4 App ID 14761

10 μm and 15 μm also available-please contact your authorized Phenomenex distributor.

3 μm, 4 μm & 5 μm analytical and Preparative Columns (mm) SecurityGuard™ Cartridges (mm)

50 x 4.6 150 x 4.6 250 x 4.6 250 x 10 250 x 21.2 4 x 3.0* 10 x 10‡ 15 x 21.2**Phases

5 μm C4 300 Å I 00B-4167-E0 I 00F-4167-E0 I 00G-4167-E0 I 00G-4167-N0 I 00G-4167-P0 AJO-4330 I AJO-7225 I AJO-7231 5 μm C5 300 Å 00B-4052-E0 00F-4052-E0 00G-4052-E0 00G-4052-N0 00G-4052-P0 AJO-4327 AJO-7371 —5 μm C18 300 Å 00B-4053-E0 00F-4053-E0 00G-4053-E0 00G-4053-N0 00G-4053-P0 AJO-4321 AJO-7224 AJO-72304 μm Proteo 90 Å 00B-4396-E0 00F-4396-E0 00G-4396-E0 00G-4396-N0 00G-4396-P0-AX AJO-6074 AJO-7275 AJO-78423 μm C18 300 Å — 00F-4263-E0 00G-4263-E0 — — AJO-4321 — —


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PROtEIn/PEPtIDE SIZE EXCLUSIOn/FILtRAtIOn


BioSep™ SEC-SEconomical protein/peptide size exclusion/filtration

• Characterize aggregates, dimers, and enzyme activity • Purify protein/peptide mixtures with wide molecular weight distribution • Excellent stability, reproducibility, & loading capacity

BioSep™ SEC-S2000Tosoh Bioscience

TSK-GEL® 2000SW

Bio-Rad

Bio-Sil® SEC 125

Agilent Technologies

ZORBAX® GF-250


TSK-GEL® 3000SW

Bio-Rad

Bio-Sil® SEC 250


ZORBAX® GF-450


TSK-GEL® 4000SW

Bio-Rad

Bio-Sil® SEC 400


*Bio-Rad protein standard was reconstituted according to manufacturer's instructions.

4 6 8 10 12 14

6

2

1 3

45

7

min

Protein Standards Column: BioSep-SEC-S3000

Dimensions: 300 x 7.8 mm

Part No.: 00H-2146-K0

Mobile Phase: 50 mM PO4 buffer, pH 6.8

Flow Rate: 1 mL/min

Detection: UV @ 280 nm

Sample: Bio-Rad protein standard, *20 µL injected

1. High MW Aggregates

2. Thyroglobulin

3. IgA

4. IgG

5. Ovalbumin

6. Myoglobin

7. Cyanocobalamin

App ID 5178


Stainless Steel Columns (mm): narrow bore analytical Preparative

300 x 4.6 300 x 7.8 600 x 7.8 300 x 21.2 600 x 21.2 Phases2000 I 00H-2145-E0 00H-2145-K0 I 00K-2145-K0 00H-2145-P0 I 00K-2145-P0

3000 00H-2146-E0 00H-2146-K0 00K-2146-K0 00H-2146-P0 00K-2146-P0

4000 00H-2147-E0 00H-2147-K0 00K-2147-K0 00H-2147-P0 00K-2147-P0

Stainless Steel Guards (mm): SecurityGuard™

narrow bore Express analytical Preparative Cartridges 30 x 4.6 35 x 7.8 75 x 7.8 60 x 21.2 4 x 3.0 mm Phases

2000 I 03A-2145-E0 03Q-2145-K0 03C-2145-K0 03R-2145-P0 AJ0-4487

3000 03A-2146-E0 03Q-2146-K0 03C-2146-K0 03R-2146-P0 AJ0-4488

4000 03A-2147-E0 03Q-2147-K0 03C-2147-K0 03R-2147-P0 AJ0-4489

SecurityGuard™Cartridges require universal holder Part No.: KJO-4282

for ID: 3.2-8.0 mm

/10pk


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Acid Amino Analysis using EZ:faast™

• Analyze over 60 free amino acids and related compounds • Sample preparation and derivatization completed in 7 minutes • No plasma or urea removal required • Chromatographic analysis is complete in less than 8 minutes • Great sample purity and improved reproducibility • One of the most economical amino acid analysis products with reagents and supplies for the analysis of 384 samples • Compatible with GC/FID, GC/MS and LC/MS systems

Amino Acid Standards Amino Acids in Human SerumApp ID 14168 App ID 14169

Kit: EZ:faast GC/FID Free (Physiological) Amino Acid Kit Part No.: KG0-7165 Injection: Split 1:15 @ 250 °C, 2.5 µL Carrier Gas: Helium 1.5 mL/minute (60 kPa) @ 110 °CPressure Rise: 6 kPa/min Oven Program: 30 °C/min from 110 °C to 320 °C, hold at 320 °C for 1 minute Detector: FID @ 320 °C Sample: Equimolar mixture of derivatized amino acid standards (10 nmol each) 1. Alanine 18. a-Aminoadipic acid 2. Glycine 19. Cysteine 3. a-Aminobutyric acid 20. a-Aminopimelic acid 4. Valine 21. Glutamine 5. b-Aminoisobutyric acid 22. Homocysteine 6. Norvaline 23. Ornithine 7. Leucine 24. Glycine-proline (dipeptide) 8. Isoleucine 25. Lysine 9. Threonine 26. Histidine 10. Serine 27. Hydroxylysine (2 isomers) 11. Proline 28. Tyrosine 12. Asparagine 29. Proline-hydroxyproline (dipeptide) 13. Aspartic acid 30. Tryptophan 14. Methionine 31. Cystathionine 15. 4-Hydroxyproline 32. Cystine 16. Glutamic acid 33. Homocystine 17. Phenylalanine

Kit: EZ:faast GC/FID Free (Physiological) Amino Acid Kit Part No.: KG0-7165 Injection: Split 1:15 @ 250 °C, 2.5 µL Carrier Gas: Helium 1.5 mL/minute (60 kPa) @ 110 °C Pressure Rise: 6 kPa/min Oven Program: 30 °C/min from 110 °C to 320 °C, hold at 320 °C for 1 minute Detector: FID @ 320 °C Sample: Derivatized amino acids in human serum (0.1 mL). Norvaline is the internal standard added at a concentration of 200 µmol/L 1. Alanine 13. Methionine 2. Glycine 14. 4-Hydroxyproline 3. a-Aminobutyric acid 15. Glutamic acid 4. Valine 16. Phenylalanine 5. Norvaline 17. Glutamine 6. Leucine 18. Ornithine 7. Isoleucine 19. Lysine 8. Threonine 20. Histidine 9. Serine 21. Tyrosine 10. Proline 22. Tryptophan 11. Asparagine 23. Cystine 12. Aspartic acid

2

0

1 2

3 4

5

6 7 8

9

10

11 12

13 14

15 16

18

19

20

21

22

23

24

25

26

27

28 30

31 32

33

29

17

25

50

75

Volta

ge [m

V]

3 4 5 6 7 min

15 MInUtE AMInO ACID AnALYSIS

ORDERING INFORMATION Amino Acid Analysis KitsEach kit includes: ZB-AAA GC column, or AAA LC column, sample prep and derivatization reagents, sample prep vials, AA standards, SPE pipette tips, vial rack, autosampler vials with inserts with MS kits, and microdispenser.

Part No. Description Unit Price

KG0-7165 GC/FID Free (Physiological) Amino Acid Analysis Kit ea

KG0-7166 GC/MS Free (Physiological) Amino Acid Analysis Kit ea

KG0-7167 GC/FID Protein Hydrolysate Kit ea

KG0-7168 GC/MS Protein Hydrolysate Kit ea

KH0-7337 LC/MS Free (Physiological) Amino Acids Kit with 250 x 2.0 mm column ea

KH0-7338 LC/MS Free (Physiological) Amino Acids Kit with 250 x 3.0 mm column ea

KH0-7339 LC/MS Protein Hydrolysates Kit with 250 x 2.0 mm column ea

KH0-7340 LC/MS Protein Hydrolysates Kit with 250 x 3.0 mm column ea

– EZ:faast is in compliance with European Directive 98/79/EC for In Vitro Diagnostics.


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Ap

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648

Column: Rezex ROA-Organic AcidDimensions: 150 x 7.8 mm

Part No.: 00F-0138-K0Mobile Phase: 0.005 N Sulfuric Acid

Flow Rate: 0.6 mL/minDetection: RI @ 40 °C

Temperature: 60 °CSystem: Shimadzu™ Prominence™ LC-20A SystemSample: 1. Dp4+ 5. Lactic Acid

2. Dp3 6. Glycerol3. Maltose 7. Acetic Acid4. Glucose 8. Ethanol

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FOOD AnD BEVERAGE AnALYSIS

Rezex™ HPLC Columns for Carbohydrate, Oligosaccharide, and Organic Acid Analysis • Analyze samples from foods, beverages, fermentation broths, and other complex matrices • Fast and easy bioethanol analysis • Recommended alternative to Bio-Rad Aminex®, Waters Sugar-Pak™, and Supelco Supelcogel™

Sugars

Baseline Separation of Critical Sample ComponentsDue to improved resolution

vs.Sugars14977 & 14978

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Rezex RCM

APP ID 14977

Great Peak Shape

vs.Sugars14977 & 14978

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Bio-Rad AminexHPX-87C

APP ID 14978

ORDERING INFORMATION Columns Guards SecurityGuard™Cartridges (mm)

4 x 3.0 Cross Ionic Description Part No. Linkage Form Size (mm) Price Part No. Size (mm) Price /10pk

RCM-Monosaccharide I 00H-0130-K0 I 8 % I Calcium I 300 x 7.8 I 03B-0130-K0 I 50 x 7.8 I AJ0-4493

RHM- Monosaccharide 00H-0132-K0 8 % Hydrogen 300 x 7.8 03B-0132-K0 50 x 7.8 AJ0-4490

RAM-Carbohydrate 00H-0131-K0 8 % Silver 300 x 7.8 — — AJ0-4491

RSO-Oligosaccharide 00P-0133-N0 4 % Silver 200 x 10.0 03R-0133-N0 60 x 10.0 AJ0-4491

RNO-Oligosaccharide 00P-0137-N0 4 % Sodium 200 x 10.0 03R-0137-N0 60 x 10.0 —

RPM-Monosaccharide 00H-0135-K0 8 % Lead 300 x 7.8 03B-0135-K0 50 x 7.8 AJ0-4492 (for USP procedure) 00D-0135-K0 8 % Lead 100 x 7.8 03B-0135-K0 50 x 7.8 AJ0-4492

RNM-Carbohydrate 00H-0136-K0 8 % Sodium 300 x 7.8 03B-0136-K0 50 x 7.8 —

ROA-Organic Acid 00H-0138-K0 8 % Hydrogen 300 x 7.8 03B-0138-K0 50 x 7.8 AJ0-4490

ROA-Organic Acid 00F-0138-K0 8 % Hydrogen 150 x 7.8 03B-0138-K0 50 x 7.8 AJ0-4490

RKP-Potassium 00H-3252-K0 8 % Potassium 300 x 7.8 — — —

RFQ-Fast Acid 00D-0223-K0 8 % Hydrogen 100 x 7.8 03B-0223-K0 50 x 7.8 AJ0-4490

RCU-USP Sugar Alcohols Column 00G-0130-D0 8 % Calcium 250 x 4.0 03A-0130-D0 30 x 4.0 AJ0-4493for ID: 3.2-8.0 mm

SecurityGuard™ Analytical Cartridges require universal holder Part No.: KJO-4282

Conditions for all columns:Dimensions: 300 x 7.8 mm

Mobile Phase: Water


Detection: ELSD

Temperature: 80 °C

Sample: 1. Sucrose 2. Glucose 3. Galactose 4. Fructose 5. Sorbitol

Bioethanol Fermentation Monitoring

Bioethanol Analysis

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www.phenomenex.comPhenomenex products are available worldwide.For the distributor in your country, contact Phenomenex USA, International Department at [email protected]

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HPLC • GC • SPE - Separationsseparations.co.za/fileadmin/themes/default/pdf/2... · HPLC SPE Strata ® Accessories BioSeparations PREP / Process GC 2 Strata Impact Precipitation

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