HPLC Catalog 2009

Orochem Technologies Inc.

Our History Orochem Technologies Inc., established in 1996, is a biotech company that serves the bioanalysis, genomics, drug discovery, and the proteomics markets. Orochem Technologies Inc. is dedicated to the development of value-added medical devices that feature unique components, custom surfaces, specialized membranes, and highly selective solid phase sorbents integrated into formats that enable high throughput sample preparation and screening. Moving beyond its established reputation as the pre-eminent supplier of sample preparation tools, the acquisition of Column Engineering Inc in 2006 provided an opportunity for us to service the varied HPLC market. Since then Orochem Technologies has greatly broadened its expertise and manufacturing capabilities in HPLC products and services. From Silica synthesis to specialized bonded phase development, Orochem products service the analytical, semi-prep and preparative needs of pharmaceutical products, contract research, API development and the genomic and proteomics applications. Our goal is to provide the best value to our customers, innovation in integrated processes for analytical and prep chromatography, high quality, low cost, and prompt services. Discover the benefits of dealing directly with the column experts contact us today!

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Content

HPLC COLUMNS

Our History Mission Statem ent Ordering Inform ation HPLC Flow Chart Checking a Catalog Item EpitomizeTM Chiral Column GazelleTM 1.7um UHPLC Colum n Matrix TM HPLC Colum n Monitor TM HPLC Colum n Prestige TM HPLC Column Reliachrom TM ReliasilTM HPLC Column OroPureTM HPLC Colum n OroSym TM HPLC Column OroSilTM HPLC Column OroStar TM HPLC Column Nuclesil Guard Cartridges Prep/Sem i-Prep Column Test Mix Orochem Colum ns Cross Referenced w ith Industry Columns Industry Columns Cross Referenced with Orochem Columns Contact Inform ation

1 3 4 5 6 7 10 11 14 18 20 22 26 27 33 35 36 37 44 45 49 54 61

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Mission Statement At Orochem Technologies, the core expertise is in the development and manufacture of customized individual and high throughput sample preparation devices, unique chemistries for columns, analytical, preparatory, and chiral chromatography. We are driven to understand and offer tools for parallel processing, scale-up and process chromatography. Our mission is to offer a one-shop to integrate indigenously developed chemistries for separations into custom plastic molded or machined devices. Knowledge of chemical biochemical processes assay sample preparation system allows us to integrate and support sample prep and chromatography systems into automated robotic workstations.

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Ordering Information

MAIL TO:

Orochem Technologies Inc. 331 Eisenhower Ln S Lombard, IL 60148 Tel.: 630.916.0225 Fax: 630.916.0250 Email: [email protected] om Web: www. orochem.com

HPLC COLUMNS

PRICE: SHIPPING: WARRANTY:

Quoted in U.S . Dollars and subject to change without notice UPS Ground or FedE x unless otherwis e specified All products are warranted t o be free from defects in materials and workmanship.

TERMS: RETURNS:

FOB point of shipment, Net 30 days on approval of credit. All returns are subject to 15% re-stoc king f ee. For any damaged goods, OROCHEM TECHNOLOGIES, INC. must be informed within 7 days to receive proper credit or replacement with a R eturn Godds Authorization. Goods must be in their original pac kaging to obtain proper credit. Due t o the nature of pac kaging however, some goods may not be returnable.

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HPLC FLow Chart

SOLUBILITY

MODE

PHASEAmino, Cyano RELIASIL Silica

PAGE

Organic-soluble only

NORMAL PHASE

RELIASIL, MONITOR

14, 22

Soluble in H2O or organic C18, C8, etc.,

MW < 2000Aqueous-soluble only

REVERSE PHASE CHROMATOGRAPHY

RELIASIL, MONITOR, MATRIX Phenyl Cyano

11,14,

Strong Cation or Anion Exchanger Aqueous-soluble only

ION-EXCHANGE

RELIASIL

22

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Checking a Catalog ItemCheck your desired product in the ordering information section Ordering information

Column SizePhase 3 m Silica 5 m C18 50 x 2.0 mm R3BE-100 R5BE-121 50 x 4.6 mm R3BI-100 R5BI-121

Particle Size

HPLC COLUMNS

Packing Material Type R:Reliasil M: Monitor

Part Number

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Orochem Technologies Inc.EpitomizeTM Chiral CSP1 ColumnsEpitomizeTM CSP1 Chiral columns are based on cellulose tris (3,5-dimethylphenylcarbamate) coated on 5um spherical silica with 10um and 20um available upon special request. Typical Applications: Four applications have been chosen to show the excellent chiral separation selectivity of the EpitomizeTM CSP1 material when compared to a popular competitive material. The EpitomizeTM CSP1 Chiral material used for these comparisons was a 10um material and the examples show excellent separations and efficiencies even when compared to the 5um High Efficiency version from the competition. The applications selected are 1) The separation of the isomers of Atropine 2) The separation of the isomers of Proglumide 3) The separation of the isomers of Flavanone 4) The separation of the isomers of Homatropine

Atropine on 5um competitive material

Column Conditions for Atropine Separations Column Size: 250 x 4.6mm Mobile Phase: Hexane/Ethanol (90/10) Flow Rate: 1.0mL/min Injection Volume: 10um

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EpitomizeTMChiral Column

Proglumide on 5um competitive material

HPLC COLUMNS

Column Conditions for Proglumide Separations Column Size: 250 x 4.6mm Mobile Phase: Hexane/Ethanol (90/10) + 0.1% TFA Flow Rate: 1.0mL/min Injection Volume: 10um

Flavanone on 5um competitive material

Column Conditions for Flavanone Separations Column Size: 250 x 4.6mm Mobile Phase: Hexane/Ethanol (90/10) Flow Rate: 1.0mL/min Injection Volume: 10um

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Homatropine on 5um competitive materia

Column Conditions for Homatropine Separations Column Size: 250 x 4.6mm Mobile Phase: Hexane/Ethanol (90/10) Flow Rate: 1.0mL/min Injection Volume: 10um

Ordering InformationPopular DimensionsPhase Epitomize CSP1 50x2.0 E5BE-927 50x4.6 E5BI-927 150x4.6 E5FI-927 250x 4.6 E5HI- 927

Unique sizes available

Phase Epitomize CSP1

30 x 4.6 mm E5AI-927

100 x 4.6 mm E5DI-927

30 x 3 mm E5AG-927

50 x 3 mm E5BG-927

Phase Epitomize CSP1

100 x 3 mm E5DG-927

125 x 3.0 mm E5EG-927

150 x 3 mm E5FG-927

150 x 2 mm E5FE-927

250 x 3 mm E5HG-927

300 x 3.9 mm E5IS-927

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GazelleTM 1.7um UHPLC Column

GazelleTM 1.7um UHPLC ColumnGazelleTM is designed by Orochem Technologies, especially to yield higher sensitivity and reproducibility in less run time. Orochem uses a proprietary silica type A manufacturing process that yields a narrow particle size and pore size distribution, which gives you consistent and reproducible results. The hydrophobic spherical surface is also stable over a wide pH range (2-8) to allow for excellent method flexibility. Advantages: Good Resolution Faster Analysis High Sensitivity Low band spreading Saves Solvent

HPLC COLUMNS

Features: Surface Area: 190m2/g Pore Size: 100A Pore Volume: 0.6 pH Range: 2-8 Particle Size: 1.7um

Retention of Non-Polar CompoundsColumn: Gazelle 1.7um C18 50 x 2.1 mm Mobile Phase: 60% Acetonitrile/40% Water

Temperature: 30 oC Flow Rate: 0.2 ml/min Detection: 254uv0.5 1.2 1.7 2.9 3.8

Test Mix 1. Uracil 2. Acetophenone 3. Methyl Benzoate 4. Toluene 5. Naphthalene

Ordering InformationPhase Silica C 18 50 x 2.1 mm G17BQ-930 G17BQ-931 30 x 2.1 mm G17AQ-930 G17AQ-931

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2

3

4

5

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Orochem Technologies Inc.Matrix TM HPLC ColumnMatrixTM C18 MatrixTM uses base-deactivated ultra-high purity silica, densely bonded to produce an inert material ideally suited for the analysis of basic compounds. The resulting hydrophobic surface is also stable over a wide pH range (2-9) to allow for excellent method flexibility. MatrixTM C18 AQ MatrixTM C18 AQ has been specifically designed for the analysis of polar analytes in high aqueous mobile phases. Matrix TM C18 AQ features the traditional C18 ligand interspersed with hydrophilic end-capping groups. The resulting material is easily wettable, and unlike conventional C18 materials, does not exhibit retention loss in high aqueous conditions.

Surface Area: 300m2/g Pore Size: 100A Pore Volume: 0.95 pH Range: 2-8 Particle Size: 3um, 5um, 10um

Matrix C18 250 x 4.6 mm Mobile Phase: 20% Acetonitrile 80% 0.05M Phosphate Buffer, pH 2.5 Flow Rate: 1.0 mL/min Sample: 1. Pseudoephedrine 2. Lidocaine 3. Pyrilamine 4. Chlorpheniramine

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MatrixTM HPLC Column

Mobile Phase: 69% Water, 28% MeOH, 3%Acetonitrile Flow Rate: 1.2 mL/min Sample: 1. Acetaminophen 2. Caffeine 3. Aspirin 4. Benzoic Acid

HPLC COLUMNS

Benefits: 1. Ultra-High Purity Silica 2. AQ-C18 Version for high-aqueous conditions 3. Excellent value 4. pH-stable from 2-9 5. Custom Column Geometries available

Ordering InformationPopular DimensionsPhase 3 m NH2 3 m C8 3 m AQ 3 m C18 50 x 2.0 mm R3BE - 138 R3BE - 135 R3BE - 171 R3BE - 136 50 x 4.6 mm R3BI - 138 R3BI - 135 R3BI - 171 R3BI - 136 150 x 4.6 mm R3FI - 138 R3FI - 135 R3FI - 171 R3FI - 136

Phase 5 m NH2 5 m C8 5 m C18 AQ 5 m C18

50 x 2.0 mm R5BE - 137 R5BE - 130 R5BE - 170 R5BE - 131

50 x 4.6 mm R5BI - 137 R5BI - 130 R5BI - 170 R5BI - 131

150 x 4.6 mm R5FI - 137 R5FI - 130 R5FI - 170 R5FI - 131

250 x 4.6 mm R5HI-137 R5HI-130 R5HI-170 R5HI-131

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Unique size available:Phase 3 m NH2 3 m C8 3 m AQ 3 m C18 30 x 4.6 mm R3AI-1 38 R3AI-1 35 R3AI-1 71 R3AI-1 36 100 x 4.6 mm R3DI-1 38 R3DI-1 35 R3DI-1 71 R3DI-1 36 150 x 2 mm R3FE-138 R3FE-135 R3FE-171 R3FE-136

Phase 3 m NH2 3 m C8 3 m AQ 3 m C18

30 x 3 mm R3AG-138 R3AG-135 R3AG-171 R3AG-136

50 x 3 mm R3BG-138 R3BG-135 R3BG-171 R3BG-136

100 x 3 mm R3DG-138 R3DG-135 R3DG-171 R3DG-136

125 x 3.0 mm R3EG-138 R3EG-135 R3EG-171 R3EG-136

150 x 3 m m R3FG-138 R3FG-135 R3FG-171 R3FG-136

250 x 3 m m R3HG-138 R3HG-135 R3HG-171 R3HG-136

300 x 3.9 mm R3IS-138 R3IS-135 R3IS-171 R3IS-136


30 x 4.6 mm R5AI-137 R5AI-130 R5AI-170 R5AI-131

100 x 4.6 mm R5DI-137 R5DI-130 R5DI-170 R5DI-131

150 x 2 mm R5FE-137 R5FE-130 R5FE-170 R5FE-131


30 x 3 mm R5AG-137 R5AG-130 R5AG-170 R5AG-131

50 x 3 mm R5BG-1 37 R5BG-1 30 R5BG-1 70 R5BG-1 31

100 x 3 mm R5DG-137 R5DG-130 R5DG-170 R5DG-131

125 x 3.0 mm R5EG-1 37 R5EG-1 30 R5EG-1 70 R5EG-1 31

150 x 3 mm R5FG-137 R5FG-130 R5FG-170 R5FG-131

250 x 3 mm R5HG-137 R5HG-130 R5HG-170 R5HG-131

300 x 3.9 mm R5IS-1 37 R5IS-1 30 R5IS-1 70 R5IS-1 31

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MonitorTM HPLC Column

MonitorTM HPLC ColumnMonitorTM is a top-quality phase, providing excellent stability and reproducibility, and minimal secondary interaction. Monitor is a perfect choice for reversed phase separations requiring thoroughly base-deactivated columns, and excels in challenging pharmaceutical and peptide separations. We developed MonitorTM expressly for the chromatography of difficult-to-separate samples. The spherical base silica is made using a two-step hydrolysis of high-purity TEOS under tightly controlled conditions. By using only high purity reactants to manufacture the base gel, excellent base-deactivation characteristics are achieved, and our reaction conditions are optimized to yield smooth pores with a narrow pore size distribution. Elimination of fines in the classification step results in columns of exceptionally low back pressure. Monitor columns have unsurpassed reduced surface activity, and excel in the separation of basic compounds, peptides, and amino acids. Manufactured by Orochem Technologies Inc., using state-of-the-art technology; for reversed phase separations with minimal secondary interactions, Monitor Columns are an excellent alternative for most C18 applications requiring base-deactivated columns, especially for difficult pharmaceutical and peptide applications. Surface Area: 300m2/g Pore Size: 100A Pore Volume: 0.9 pH Range: 2-8 Particle Size: 3um, 5um

HPLC COLUMNS

Pyridine & Phenol Monitor 5m m C18; 150x4.6mmID; 1650psi Zorbax Rx C18; 150x4.6mmID; 1800psi

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Inertsil ODS-2: 150x4.6mmID; 2350psi

Symmetry C18; 150x3.9mmID; 2500psi

Kromasil C18; 150x4.6mmID; 1600psi

Mobile Phase: 60% 0.01M KH2PO4 to pH7 with KOH/40% Methanol Flow Rate: 1mL./min. Detection: UV254 Temp.: Ambient All columns packed with 5Mm C18 material as specified. Each horizontal tick mark is equivalent to one minute

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MonitorTM HPLC Column

Chlorpheniramine Maleate

Mobile Phase: 60:40 50mM KH2PO4, pH3.0:Acetonitrile Column: Monitor 5m m C18, 150x4.6mmID

HPLC COLUMNS

Benefits Improved Peak Shape for Basic and Acidic Compounds Very Reproducible Results Low Back Pressure For Longer Column Life Excellent reproducibility Custom Column Geometries available

Ordering InformationPopular Dimensions:

Phase 3m 100A Silica 3 m 100A C8 3 m 100A C18

50 x 2.0 mm M3BE-003 M3BE-002 M3BE-001

50 x 4.6 mm M3BI-003 M3BI-002 M3BI-001

150 x 4.6 mm M3FI-003 M3FI-002 M3FI-001

Phase 5 m 100A Silica 5 m 100A C8 5 m 100A C18

50 x 2.0 mm M5BE-004 M5BE-006 M5BE-005

50 x 4.6 mm M5BI-004 M5BI-006 M5BI-005

150 x 4.6 mm M5FI-004 M5FI-006 M5FI-005

Microbore/Minibore (in mm) 3 m 100A Silica 3 m 100A C8 3 m 100A C18

50x1.0 M3BD-003 M3BD-002 M3BD-001

100x1.0 M3DD-003 M3DD-003 M3DD-001

150x1.0 M3FD-003 M5FD-002 M3FD-001

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Microbore/Minibore (in mm) 5 m 100A Silica 5 m 100A C8 5 m 100A C18

50x1.0 M5BG-004 M5BG-006 M5BG-005

100x1.0 M5DG-004 M5DG-006 M5DG-005

150x1.0 M5FG-004 M5FG-006 M5FG-005

250x1.0 M5HG-004 M5HG-006 M5HG-005

Prep/Se mi-Prep. 5 m 100A S ilica 5 m 100A C8 5 m 100A C18

100x10 mm M5DL-00 4 M5DL-00 6 M5DL-00 5

250x10.0 mm M5HL-004 M5HL-006 M5HL-005

50x20 mm M5BM-004 M5BM-006 M5BM-005

100x21 mm M5DM-004 M5DM-006 M5DM-005

250X2 1 mm M5HM-004 M5HM-006 M5HM-005

250X5 0 mm M5HO-00 4 M5H0-00 6 M5H0-00 5

250x4.6 mm M5HI-004 M5HI-006 M5HI-005

Unique sizes available


30 x 4.6 mm M3AI-003 M3AI-002 M3AI-001

100 x 4.6 mm M3DI-003 M3DI-002 M3DI-001

150 x 2 mm M3FE-003 M3FE-002 M3FE-001


30 x 3 mm M3AG-003 M3AG-002 M3AG-001

50 x 3 mm M3BG-003 M3BG-002 M3BG-001

100 x 3 mm M3DG-003 M3DG-002 M3DG-001

125 x 3.0 mm M3EG-003 M3EG-002 M3EG-001

150 x 3 mm M3FG-003 M3FG-002 M3FG-001

250 x 4.6 mm M5HI-004 M5HI-005 M5HI-006


30 x 4.6 mm M5AI-004 M5AI-005 M5AI-006

100 x 4.6 mm M5DI-004 M5DI-005 M5DI-006

150 x 2 mm M5FE-004 M5FE-005 M5FE-006

30 x 3 mm M5AG-004 M5AG-005 M5AG-006

50 x 3 mm M5BG-004 M5BG-005 M5BG-006

100 x 3 mm M5DG-004 M5DG-005 M5DG-006


125 x 3.0 mm M5EG-004 M5EG-005 M5EG-006

150 x 3 mm M5FG-004 M5FG-005 M5FG-006

250 x 3 mm M5HG-004 M5HG-005 M5HG-006

300 x 4.0 mm M5IS-004 M5IS-005 M5IS-006

250 x 4.6 mm M5HI-004 M5HI-005 M5HI-006

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PrestigeTM HPLC Column

PrestigeTM HPLC ColumnPrestigeTM is Orochem Technologies next-generation of highly base-deactivated sorbents. Double end-capping and narrow particle size range contributes to superior base-deactivation and low backpressure. Surface Area: 320m2/g Pore Size: 100A Pore Volume: 0.9 pH Range: 2-8.5 Particle Size: 3um, 5um, 10um


HPLC COLUMNS

Popular dimensionPhase 3 m 100A C8 3 m 100A C18 50x2.0 mm P3BE-072 P3BE-073 50x2.0 mm P5BE-071 P5BE-070 50x4.6 mm P3BI-072 P3BI-073 50x4.6 mm P5BI-071 P5BI-070 150x4.6 mm P3FI-072 P3FI-073 150x4.6 mm P5FI-071 P5FI-070 250x4.6 mm P5HI-071 P5HI-070

Phase 5 m 100A C8 5 m 100A C18

Microbore/Minibore (in mm) 3 m 100A C8 3 m 100A C18

50x1.0 P3BD-072 P3BD-073

100x1.0 P3DD-072 P3DD-073

150x1.0 P3FD-072 P3FD-073

100x2.0 P3DE-072 P3DE-073

150x2.0 P3FE-072 P3FE-073

Microbore / Minibore (in mm) 5 m 100A C8 5 m 100A C18

50x1.0 P5BD-071 P5BD-070

100x1.0 P5DD-071 P5DD-070

150x 1.0 P5FD-071 P5FD-070

250x1.0 P5HD-071 P5HD-070

100x 2.0 P5DE-071 P5DE-070

150x2.0 P5DE-071 P5DE-070

250x2.0 P5HE-071 P5HE-070

Standard/Mid Bor e(in mm) 3 m 100A C8 3 m 100A C18

50x3.0 P3BG-072 P3BG-073

100x3.0 P3DG-072 P3DG-073

150x3.0 P3FG-072 P3FG-073

30x4.6 P3AI-072 P3AI-073

100x4.6 P3DI-072 P3DI-073

Standard/Mid Bor e(in mm) 5 m 100A C8 5 m 100A C18

50x3.0 P5BG-071 P5BG-070

100x3.0 P5DG-071 P5DG-070

150x3.0 P5FG-071 P5FG-070

250x3.0 P5HG-071 P5HG-070

30x4.6 P5AI-071 P5AI-070

100x4.6 P5DI-071 P5DI-070

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Unique sizes available:

Phase 3 m 100A C8 3 m 100A C18

30 x 4.6 mm P3AI-072 P3AI-073

150 x 2 mm P3FE-072 P3FE-073

30 x 3 mm P3AG-072 P3AG-073

50 x 3 mm P3BG-072 P3BG-073

Phase 3 m 100A C8 3 m 100A C18

100 x 3 mm P3DG-072 P3DG-073

125 x 3.0 mm P3EG-072 P3EG-073

150 x 3 mm P3FG-072 P3FG-073

300 x 3.9 mm P3IS-072 P3IS-073

Phase 5 m 100A C8 5 m 100A C18

30 x 4.6 mm P5AI-071 P5AI-070

150 x 2 mm P5FE-071 P5FE-070

30 x 3 mm P5AG-071 P5AG-070

50 x 3 mm P5BG-071 P5BG-070

Phase 5 m 100A C8 5 m 100A C18

100 x 3 mm P5DG-071 P5DG-070

125 x 3.0 mm P5EG-071 P5EG-070

150 x 3 mm P5FG-071 P5FG-070

250 x 3 mm P5HG-071 P5HG-070

300 x 4.0 mm P5IS-071 P5IS-070

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ReliachromTM HPLC Column

ReliachromTMReliachromTM was designed by Orochem Technologies specifically to yield columns with a high plate count. We use a proprietary type A silica manufacturing process which yields a Gaussian particle distribution, narrow pore-size distribution, and strong resistance to high pH. The result is a material that is perfect for packing high-efficiency columns for general utility. Surface Area: 190m2/g Pore Size: 90A Pore Volume: 0.55 pH Range: 2-8.5 Particle Size: 3um, 5um, 10um

HPLC COLUMNS

Ordering InformationPhase 3 m Silica 3 m 3 m 3 m 3 m 3 m 3 m C1 NH2 SCX SAX Phenyl CN 50 x 2.0 mm RC3BE-930 RC3BE-933 RC3BE-937 RC3BE-940 RC3BE-942 RC3BE-944 RC3BE-946 RC3BE-949 RC3BE-952 50 x 4.6 mm RC3BI-930 RC3BI-933 RC3BI-937 RC3BI-940 RC3BI-942 RC3BI-944 RC3BI-946 RC3BI-949 RC3BI-952 100 x 4.6mm RC3DI-930 RC3DI-933 RC3DI-937 RC3DI-940 RC3DI-942 RC3DI-944 RC3DI-946 RC3DI-949 RC3DI-952 150 x 4.6 mm RC3FI-930 RC3FI-933 RC3FI-937 RC3FI-940 RC3FI-942 RC3FI-944 RC3FI-946 RC3FI-949 RC3FI-952 250 x 4.6 mm RC3HI-930 RC3HI-933 RC3HI-937 RC3HI-940 RC3HI-942 RC3HI-944 RC3HI-946 RC3HI-949 RC3HI-952

3 m C8 3 m C18

Phase 5 m Silica 5 m C1 5 m C4 5 m 5 m 5 m 5 m 5 m 5 m 5 m NH2 SCX SAX Phenyl CN C8 C18

50 x 2.0 mm RC5BE-931 RC5BE-934 RC5BE-935 RC5BE-938 RC5BE-941 RC5BE-943 RC5BE-945 RC5BE-947 RC5BE-950 RC5BE-953

50 x 4.6 mm RC5BI-931 RC5BI-934 RC5BI-935 RC5BI-938 RC5BI-941 RC5BI-943 RC5BI-945 RC5BI-947 RC5BI-950 RC5BI-953

100 x 4.6mm RC5DI-931 RC5DI-934 RC5DI-935 RC5DI-938 RC5DI-941 RC5DI-943 RC5DI-945 RC5DI-947 RC5DI-950 RC5DI-953

150 x 4.6 mm RC5FI-931 RC5FI-934 RC5FI-935 RC5FI-938 RC5FI-941 RC5FI-943 RC5FI-945 RC5FI-947 RC5FI-950 RC5FI-953

250 x 4.6 mm RC5HI-931 RC5HI-934 RC5HI-935 RC5HI-938 RC5HI-941 RC5HI-943 RC5HI-945 RC5HI-947 RC5HI-950 RC5HI-953

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Phase 10 m Silica 10 m C4 10 m NH2 10 m CN 10 m C8 10 m C18

100 x 10.0 mm RC9DL-140 RC9DL-141 RC9DL-142 RC9DL-143 RC9DL-145 RC9DL-147

250 x 10.0 RCHL-140 RCHL-141 RCHL-142 RCHL-143 RCHL-145 RCHL-147

100 x 21.2 RC5DM-140 RC5DM-141 RC5DM-142 RC5DM-143 RC5DM-145 RC5DM-147

250 X 21.2 RC9HM-140 RC9HM-141 RC9HM-142 RC9HM-143 RC9HM-145 RC9HM-147

250 X 50 RC9HO-140 RC9HO-141 RC9HO-142 RC9HO-143 RC9HO-145 RC9HO-147

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ReliasilTM HPLC Column

ReliasilTM HPLC ColumnReliasilTM was designed by Orochem Technologies specifically to yield high plate count. We use a proprietary type A silica manufacturing process which yields a Gaussian particle distribution, narrow pore-size distribution, and strong resistance to high pH. The result is a material that is perfect for packing high-efficiency columns for general utility. Manufactured by Orochem Technologies, Inc., ReliasilTM is an oligomerically derivatized silica gel. It is preferred by many chromatographers for its chemical stability and effectiveness in the reversed phase separations of peptides, pharmaceuticals, and derivatized amino acids.

HPLC COLUMNS22

Surface Area: 190m2/g Pore Size: 90A Pore Volume: 0.55 pH Range: 2-8.5 Particle Size: 3um, 5um, 10um Benefits 1. Reproducibility 2. Excellent Mechanical/Chemical Stability 3. Superior Efficiency 4. Wide variety of phases 5. Packed to high efficiencies 6. Rugged General-purpose material Available Phases C18 C8 C4 C1 CN (100A and 300A) Hardware Options PEEK Glass-lined Stainless Steel Custom Guard Columns.


ReliasilTM 5um C18 150 x 4.6 mm

Mobile Phase: CAN/MeOH (7.5:25) Flow Rate: 1.3 ml/min Sample: 1. Vitamin A 2. Vitamin A Acetate 3. Vitamin D2 4. Vitamin D3 5. Vitamin E 6. Vitamin E Acetate 7. Vitamin K1

Ordering InformationPopular DimensionsPhase 3m Silica 3 m C1 3 m 3 m 3 m 3 m 3 m NH 2 SCX SAX Phenyl CN 50 x 2.0 mm R3BE-100 R3BE-104 R3BE-103 R3BE-107 R3BE-108 R3BE-106 R3BE-105 R3BE-102 R3BE-101 R3BE-118 50 x 4.6 mm R3BI-100 R3BI-104 R3BI-103 R3BI-107 R3BI-108 R3BI-106 R3BI-105 R3BI-102 R3BI-101 R3BI-118 150 x 4.6 mm R3FI-100 R3FI-104 R3FI-103 R3FI-107 R3FI-108 R3FI-106 R3FI-105 R3FI-102 R3FI-101 R3BI-118

3 m C8 3 m C18 3 m ODS-1

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ReliasilTM HPLC Column

Phase 5 m Silica 5 m C1 5 m C4 5 m NH 2 5 m SCX 5 m SAX 5 m Phenyl

50 x 2.0 mm R5BE-120 R5BE-124 R5BE-123 R5BE-132 R5BE-133 R5BE-134 R5BE-117 R5BE-125 R5BE-122 R5BE-109 R5BE-121 R5BE-119

50 x 4.6 mm R5BI-120 R5BI-124 R5BI-123 R5BI-132 R5BI-133 R5BI-134 R5BI-117 R5BI-125 R5BI-122 R5BI-109 R5BI-121 R5BI-119

150 x 4.6 mm R5FI-120 R5FI-124 R5FI-123 R5FI-132 R5FI-133 R5FI-134 R5FI-117 R5FI-125 R5FI-122 R5FI-109 R5FI-121 R5FI-119

250 x 4.6 mm R5HI-120 R5HI-124 R5HI-123 R5HI-132 R5HI-133 R5HI-134 R5HI-117 R5HI-125 R5HI-122 R5HI-109 R5HI-121 R5HI-119

HPLC COLUMNS

5 m CN 5 m C8 5 m C18 PAH 5 m C18 5 m ODS-1

Phase 10 m Silica 10 m C4 10 m NH2 10 m CN 10 m C8 10 m C18

100 x 10.0 mm R9DL-140 R9DL-141 R9DL-142 R9DL-143 R9DL-145 R9DL-147

250 x 10.0 RHL-140 RHL-141 RHL-142 RHL-143 RHL-145 RHL-147

100 x 21.2 R5DM-140 R5DM-141 R5DM-142 R5DM-143 R5DM-145 R5DM-147

250 X 21.2 R9HM-140 R9HM-141 R9HM-142 R9HM-143 R9HM-145 R9HM-147

250 X 50 R9HO-140 R9HO-141 R9HO-142 R9HO-143 R9HO-145 R9HO-147

Phase 5mm 300A C4 5mm 300A C8 5mm 300A C18

50 x 2.0 R5BE-128 R5BE-127 R5BE-126

50 x 4.6 R5BI-128 R5BI-127 R5BI-126

150 x 4.6 R5FI-128 R5FI-127 R5FI-126

250 x 4.6 R5HI-128 R5HI-127 R5HI-126

24


Unique sizes available:P h as e 3 m S ili c a 3 m C1 3 m NH 2 3 m S CX 3 3 3 3 3 3 m m m m m m SA X P he n yl CN C8 C18 O D S -1 3 0 x 4 .6 m m R 3 A I- 10 0 R 3 A I- 10 4 R 3 A I- 10 3 R 3 A I- 10 7 R 3 A I- 10 8 R 3 A I- 10 6 R 3 A I- 10 5 R 3 A I- 10 2 R 3 A I- 10 1 R 3 A I- 11 8 10 0 x 4 .6 m m R 3 D I- 10 0 R 3 D I- 10 4 R 3 D I- 10 3 R 3 D I- 10 7 R 3 D I- 10 8 R 3 D I- 10 6 R 3 D I- 10 5 R 3 D I- 10 2 R 3 D I- 10 1 R 3 D I- 11 8 150 x 2 m m R 3F E - 10 0 R 3F E - 10 4 R 3F E - 10 3 R 3F E - 10 7 R 3F E - 10 8 R 3F E - 10 6 R 3F E - 10 5 R 3F E - 10 2 R 3F E - 10 1 R 3F E - 11 8 30 x 3 mm R 3A G -1 0 0 R 3A G -1 0 4 R 3A G -1 0 3 R 3A G -1 0 7 R 3A G -1 0 8 R 3A G -1 0 6 R 3A G -1 0 5 R 3A G -1 0 2 R 3A G -1 0 1 R 3B G -1 1 8

P h as e 3 m S ili c a 3 m C1 3 m NH 2 3 m S CX 3 m SA X 3 m P he n yl 3 m CN 3 m C8 3 m C18 3 m O D S -1

50 x 3 m m R 3 B G -1 00 R 3 B G -1 04 R 3 B G -1 03 R 3 B G -1 07 R 3 B G -1 08 R 3 B G -1 06 R 3 B G -1 05 R 3 B G -1 02 R 3 B G -1 01 R 3 B G -1 18

1 0 0 x 3 mm R 3 D G -1 00 R 3 D G -1 04 R 3 D G -1 03 R 3 D G -1 07 R 3 D G -1 08 R 3 D G -1 06 R 3 D G -1 05 R 3 D G -1 02 R 3 D G -1 01 R 3 D G -1 18

1 2 5 x 3. 0 m m R 3E G -1 0 0 R 3E G -1 0 4 R 3E G -1 0 3 R 3E G -1 0 7 R 3E G -1 0 8 R 3E G -1 0 6 R 3E G -1 0 5 R 3E G -1 0 2 R 3E G -1 0 1 R 3E G -1 1 8

1 50 x 3 m m R 3 F G -1 00 R 3 F G -1 04 R 3 F G -1 03 R 3 F G -1 07 R 3 F G -1 08 R 3 F G -1 06 R 3 F G -1 05 R 3 F G -1 02 R 3 F G -1 01 R 3 F G -1 18

P h a se 5 m S ilic a 5 m C 1 5 m C 4 5 5 5 5 5 5 5 5 5 m m m m m m m m m N H2 SCX S AX P h e n yl CN C8 C 18 P A H C 18 O D S -1

3 0 x 4. 6 m m R 5 A I- 12 0 R 5 A I- 12 4 R 5 A I- 12 3 R 5 A I- 13 2 R 5 A I- 13 3 R 5 A I- 13 4 R 5 A I- 11 7 R 5 A I- 12 5 R 5 A I- 12 2 R 5 A I- 10 9 R 5 A I- 12 1 R 5 A I- 11 9

1 00 x 4 . 6 m m R 5 D I -1 20 R 5 D I -1 24 R 5 D I -1 23 R 5 D I -1 32 R 5 D I -1 33 R 5 D I -1 34 R 5 D I -1 17 R 5 D I -1 25 R 5 D I -1 22 R 5 D I -1 09 R 5 D I -1 21 R 5 D I -1 19

150 x 2 mm R 5F E - 12 0 R 5F E - 12 4 R R R R R R R R R R 5F E - 12 3 5F E - 13 2 5F E - 13 3 5F E - 13 4 5F E - 11 7 5F E - 12 5 5F E - 12 2 5F E - 10 9 5F E - 12 1 5F E - 11 9

30 x 3 m m R 5A G -1 2 0 R 5A G -1 2 4 R R R R R R R R R R 5A G -1 2 3 5A G -1 3 2 5A G -1 3 3 5A G -1 3 4 5A G -1 1 7 5A G -1 2 5 5A G -1 2 2 5A G -1 0 9 5A G -1 2 1 5A G -1 1 9

P h a se 5 m S ilic a 5 m C 1 5 m C 4 5 5 5 5 5 5 5 5 5 m m m m m m m m m N H2 SCX S AX P h e n yl CN C8 C 18 P A H C 18 O D S -1

50 x 3 mm R 5 B G -1 20 R 5 B G -1 24 R 5 B G -1 23 R 5 B G -1 32 R 5 B G -1 33 R 5 B G -1 34 R 5 B G -1 17 R 5 B G -1 25 R 5 B G -1 22 R 5 B G -1 09 R 5 B G -1 21 R 5 B G -1 19

100 x 3 m m R 5D G - 12 0 R 5D G - 12 4 R 5D G - 12 3 R 5D G - 13 2 R R R R R R R R 5D 5D 5D 5D 5D 5D 5D 5D G - 13 3 G - 13 4 G - 11 7 G - 12 5 G - 12 2 G - 10 9 G - 12 1 G - 11 9

1 2 5 x 3. 0 m m R 5E G -1 2 0 R 5E G -1 2 4 R 5E G -1 2 3 R 5E G -1 3 2 R R R R R R R R 5E G -1 3 3 5E G -1 3 4 5E G -1 1 7 5E G -1 2 5 5E G -1 2 2 5E G -1 0 9 5E G -1 2 1 5E G -1 1 9

1 50 x 3 m m R 5 F G -1 20 R 5 F G -1 24 R 5 F G -1 23 R 5 F G -1 32 R 5 F G -1 33 R 5 F G -1 34 R 5 F G -1 17 R 5 F G -1 25 R 5 F G -1 22 R 5 F G -1 09 R 5 F G -1 21 R 5 F G -1 19

25 0 x 3 m m R 5 H G -1 20 R 5 H G -1 24 R 5 H G -1 23 R 5 H G -1 32 R 5 H G -1 33 R 5 H G -1 34 R 5 H G -1 17 R 5 H G -1 25 R 5 H G -1 22 R 5 H G -1 09 R 5 H G -1 21 R 5 H G -1 19

3 0 0 x 4. 0 m m R 5I S -1 20 R 5I S -1 24 R 5I S -1 23 R 5I S -1 32 R R R R R R R R 5I S -1 33 5I S -1 34 5I S -1 17 5I S -1 25 5I S -1 22 5I S -1 09 5I S -1 21 5I S -1 19

25

OroPureTM HPLC Column

OroPureTM HPLC ColumnOroPureTM C18 chemistry is designed on silica where the surface area is relatively low, which makes the retention time shorter. It may be utilized to separate, molecules which have longer retention times. If you routinely use HypersilTM BDS-C18, HypersilTM BDS-C8, ZorbaxTM XDB-C18, and the ZorbaxTM XDB-C8, OroPureTM C18, or OroPureTM C8 respectively will be a good choice to evaluate. Features: - Surface Area: 200m2/g, - Pore Size: 150A, - Particle Size: 5um

HPLC COLUMNS

Ordering InformationOroPureTMPhase 3m C8 3m C18 50 x 3.0 mm OP3BG-908 OP3BG-906 50 x 4.6 mm OP3BI-908 OP3BI-906 100 x 4.6 mm OP3DI-908 OP3DI-906 150 x 4.6 mm OP3FI-908 OP3FI-906

Phase 5m C8 5m C18

50 x 3.0 mm OP5BG-907 OP5BG-905

50 x 4.6mm OP5BH-907 OP5BH-905

125 x 3.0 mm OP5EH-907 OP5EH-905

250 x 4.6 mm OP5HH-907 OP5HH-905

50 x 4.6mm OP5BI-907 OP5BI-905

150x 4.6 mm OP5FI-907 OP5FI-905

250 x 4.6 mm OP5HI-907 OP5HI-905

OroPureTM DBPhase 3m C8 3m C18 50 x 2.0 mm OP3BG-912 OP3BG-910 50 x 4.6 mm OP3BI-912 OP3BI-910 150 x 4.6 mm OP3DI-912 OP3DI-910

Phase 5m C8 5m C18

50 x 2.0 mm OP5BG-911 OP5BG-909

50 x 4.6mm OP5BH-911 OP5BH-909

150x 4.6 mm OP5FI-911 OP5FI-909

26

Orochem Technologies Inc.OroSymTM HPLC ColumnOroSymTM C18 column series are bonded to the maximum density to have the highest hydrophobicity and thus the lowest polarity. High C18 moieties result in the lowest interaction with analytes, with silanol, and demonstrate high stability at high pH and the broadest pH range. OroSymTM columns also demonstrate cleaner separation power for isomers, with a near perfect symmetry for basic, acidic and neutral compounds. Features: Surface Area: 45m2/g Pore Size: 130A

Selectivity of Basic, Acidic and Neutral Compounds

1

2

3

4

5

6

1. p-methyl benzoic acid 2. Pyridine 3. Phenol 4. Acetophenone 5. Amino-dimethyl benzene 6. Toluene150 x 4.6 mm column at 30 oC Mobile Phase: MeOH: AcOH/ NaAc(pH 5.0)= 60 : 40 Flow Rate: 1 mL/min 20uL injection detected at 254 nm

27

OroSymTM HPLC Column

Selectivity of Basic Compounds

HPLC COLUMNS150 x 4.6 mm column at 30oC Mobile Phase: MeOH: NaH2PO4/ Na2H PO4(pH 7.0)=70 : 30 Flow Rate: 1 mL/min 10uL injection detected at 254 nm

28


Test of Relative PolaritySample: 1. Uracil 2. Pyridine 3. Phenol

Promosil C18 KPyridine/KPhenol = 0.33

MP C18 K Pyridine/KPhenol = 0.45

Luna C18 KPyridine/KPhenol = 0.5

Orosym C18 KPyridine/KPhenol = 0.53

ASB C18 KPyridine/KPhenol = 0.82

150 x 4.6 mm column at 30oC Mobile Phase: MeOH: water = 60:40 Flow Rate: 1ml/min 10uL injection detected at 254nm

29


Test of Metal EffectsSample: 1. 2, 7- dihdroxynaphthalene 2. 2, 3- dihydroxynaphthalene

HPLC COLUMNS150 x 4.6 mm column at 30oC MeOH: water =65 : 35 Flow Rate: 1 mL/min 20uL injection detected at 230 nm

30


Test of HydrophobicitySample: 1. Urasil 2. Toluene

150 x 4.6 mm column at 25oC MeOH : 25mM KH2PO4 /K2HPO4 (pH 6.0) = 80 : 20 Flow Rate: 1 mL/min u 5uL injection detected at 254 nm u

31


Test of Lifetime under pH 1.5Sample: 1. Uracil 2. Phenol 3. Nitrobenzene 4. Naphthalene

HPLC COLUMNS150 x 4.6 mm column at 50oC MeOH : 0.25% TFA (pH 1.5) = 70 : 30 Flow Rate: 1 mL/min 5uL injection detected at 254 nm

Ordering InformationPhase 3m C8 3m C18 Phase 5m C8 5m C18 100 x 4.6 mm OY3DI-922 OY3DI-920 50 x 3.0 mm OY5BG-921 OY5BG-919 50 x 4.6 mm OY3BI-922 OY3BI-920 50 x 4.6 mm OY5BI-921 OY5BI-919 150 x 2.0 mm OY3FI-922 OY3FI-920 50 x 2.0 mm OY5BH-921 OY5BH-919 50 x 3.0 mm OY3BG-922 OY3BG-920 150 x 4.6 mm OY5FI-921 OY5FI-919 125 x 3.0 mm OY5EH-921 OY5EH-919 250 x 4.6mm OY5HI-921 OY5HI-919

32


OroSilTM HPLC ColumnOroSilTM HPLC columns are uniquely designed for the separation of polar, semi-polar and nonpolar compounds at low to medium pH. The OroSilTM columns are more polar than the OroPureTM columns but are less polar than the OroStarTM HPLC column chemistry. The OroSilTM chemistry provides authentic C18 chemistries, commonly requested and used amongst chromatographers in the industry. With specialized surface treatment, the OroSilTM chemistry makes these columns compatible even with 100% aqueous mobile phase. The OroSilTM columns are known to be stable at pH 1.5-9.0 and are preferentially used against the Waters Symmetry, Inertsil C18 or ODS 3, Zorbax SB, and also the Atlantis dC18 chemistry. Features: Surface Area: 380m2/g, Pore Size: 100A, Particle Size: 5um

Figure 1: Analysis on Inertsil ODS 3 250 x 4.6mm, 5umFigure 1: Analysis on Inertsil ODS 3 250 x 4.6mm, 5um Figure 1: Analysis on Inertsil ODS 3 250 x 4.6mm, 5um

33

OroSilTM HPLC Column


Phase 5m C8 5 m C18

50 x 2.0 mm O5BE-923 N/A

50 x3.0 mm O5BG-923 O5BG-903

50 x 4.6 mm O5BI-923 O5BI-903

100 x 10 mm O5DL-923 N/A

100 x 21.2 mm O5DT-923 N/A

125 x 4.0 mm O5EH-923 O5EH-903

Phase

150 x 4.6 mm O5FI-923 O5FI-903

250 x 4.0 mm O5HH-923 O5HH-903

250 x 4.6 mm O5HI-923 O5HI-903

250 x 21.2 mm O5HT-923 N/A

250 x 50 mm O5HO-923 N/A

HPLC COLUMNS34

5m C8 5 m C18

Orochem Technologies Inc.OroStarTM HPLC ColumnOroStarTM HPLC series of columns are specially designed for the separation of polar compounds at very low to a medium pH value. OroStarTM BS chemistries utilize silica which is end-capped, contains polar groups and in addition have the highest polarity among the OroStarTM columns. These columns are extremely stable at low pH (pH=1) and are alternatives to columns used in the industry. If you use the Waters AtlantisTM, or the ZorbaxTM SB, the OroPureTM C18 or C8 columns may be good alternatives. Features: Surface Area: 200m2/g, Pore Size: 150A Particle Size: 5um

Ordering InformationPhase 3m C8 3m C18 50 x 4.6 mm OT3BI-918 OT3BI-916 100 x 4.6 mm OT3DI-918 OT3DI-916 150 x 4.6 mm OT3FI-918 OT3FI-916 50 x 2.0 mm OT3BG-918 OT3BG-916

Phase 5m C8 5m C18

50 x 2.0 mm OT5BG-917 OT5BG-915

50 x 4.6 mm OR5BH-917 OT5BH-915

125 x 3.0 mm OT5EH-917 OT5EH-915

50 x 4.6 mm OT5BI-917 OT5BI-915

150 x 4.6 mm OT5FI-917 OT5BH-915

35

NuclesilTM HPLC Column

Nucleosil

Ordering InformationStandard BorePhase 3mm 100A C8 3mm 100A C18 30x4.6 N3AI-502 N3AI-501 50x4.6 N3BI-502 N3BI-501 100x4.6 N3DI-502 N3DI-501 150x4.6 N3FI-502 N3FI-501 125x4.0 N3EH-502 N3EH-501 250x4.0 N3HH-502 N3HH-501

Microbore/minibore

HPLC COLUMNS

3mm 100A C8 3mm 100A C18

N3BE-502 N3BE-501

N3DE-502 N3DE-501

N3FE-502 N3FE-501

Standard BorePhase 5mm 100A NH2 5mm 100A CN 5mm 120A C8 5mm 100A C8 5mm 120A C18 5mm 100A C18 5mm 300A C4 5mm 300A C8 5mm 300A C18 30x4.6 N5AI-526 N5AI-525 N5AI-534 N5AI-523 N5AI-533 N5AI-521 N5AI-538 N5AI-537 N5AI-536 50x4.6 N5BI-526 N5BI-525 N5BI-534 N5BI-523 N5BI-533 N5BI-521 N5BI-538 N5BI-537 N5BI-536 100x4.6 N5DI-526 N5DI-525 N5DI-534 N5DI-523 N5DI-533 N5DI-521 N5DI-538 N5DI-537 N5DI-536 150x4.6 N5FI-526 N5FI-525 N5FI-534 N5FI-523 N5FI-533 N5FI-521 N5FI-538 N5FI-537 N5FI-536 250x4.6 N5HI-526 N5HI-525 N5HI-534 N5HI-523 N5HI-533 N5HI-521 N5HI-538 N5HI-537 N5HI-536 125x4.0 N5EH-526 N5EH-525 N5EH-534 N5EH-523 N5EH-533 N5EH-521 N5EH-538 N5EH-537 N5EH-536 250x4.0 N5HH-526 N5HH-525 N5HH-534 N5HH-523 N5HH-533 N5HH-521 N5HH-538 N5HH-537 N5HH-536

Microbore/miniborePhase 5mm 100A NH2 5mm 100A CN 5mm 120A C8 5mm 100A C8 5mm 120A C18 5mm 100A C18 5mm 300A C4 5mm 300A C8 5mm 300A C18 50x2.0 N5BE-526 N5BE-525 N5BE-534 N5BE-523 N5BE-533 N5BE-521 N5BE-538 N5BE-537 N5BE-536 100x2 N5DE-526 N5DE-525 N5DE-534 N5DE-523 N5DE-533 N5DE-521 N5DE-538 N5DE-537 N5DE-536 150x2.0 N5FE-526 N5FE-525 N5FE-534 N5FE-523 N5FE-533 N5FE-521 N5FE-538 N5FE-537 N5FE-536 250x2.0 N5HI-526 N5HI-525 N5HI-534 N5HI-523 N5HI-533 N5HI-521 N5HI-538 N5HI-537 N5HI-536

36


Guard Cartridges

Guard cartridges are protection devices for analytical and preparative HPLC columns. They function as a filter and adsorber of materials that can adversely affect the chemistry of the separation or the lifetime of the column. Their use is highly recommended when injecting anything other than pure filtered standards that elute in a reasonable number (

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