hp1050 79853C

1050 Series of HPLC Modules

Service Handbook

Agilent TechnologiesHewlett-Packard-Strasse 876337 WaldbronnGermany

Copyright Agilent Technologies 2001

All rights reserved. Reproduction, adaption, or translation without prior written permission is prohibited, except as allowed under the copyright laws.

Part No. NONE

09/2001

Printed in Germany

Warranty

The information contained in this document is subject to change without notice.

Agilent Technologies makes no warranty of any kind with regard to this material, including, but not limited to, the implied warranties or merchantability and fitness for a particular purpose.

Agilent Technologies shall not be liable for errors contained herein or for incidental or consequential damages in connection with the furnishing, performance, or use of this material.

IMPORTANT NOTE

This version of the 1050 service manual includes all sections from the 01050-90102 edition 4 (1995).

The series I opticals information (79854A MWD and G1306A DAD) and the 79853A VWD information has been removed (products went out of support during 2000).

Part numbers have been updated as of 09/2001. Contact your local Agilent support office in case of part number issues or upgrades.

The latest version of this manual is available as Adobe Acrobat Reader (PDF) version only and can be downloaded from the Agilent Technolgies web page www.agilent.com.

Contents

1 Common: General Information

This chapter provides general information about the 1050 Se-Service Handbook for 1050 Series of HPLC Modules - 09/2001 3

ries of HPLC Modules 29

Safety Information 30General 30Operation 30Safety Symbols 32

Radio Interference 33Manufacturers Declaration 33

Sound Emission 33Manufacturers Declaration 33

UV-Radiation 34Solvent Information 35

Flow Cell 35Solvents 35

1050 Introduction 36The Modules Overview 361050 Identification 38Repair Policy 38

2 Common: Electronic Information

This chapter provides common electronic information about the 1050 Series of HPLC Modules 39

Overview 41Common Main Processor Board (CMP) 42

Common 1050 Functions 42CMP Details 44Remote Control 47

Contents

Firmware Board (FIM) 53Firmware Description 534 Service Handbook for 1050 Series of HPLC Modules - 09/2001

Fluorescent Indicator Module (FIP) 55External Contacts 56Power Supply (DPS-B / DPS-A) 57

General Description 57Base Supply (DPS-B) 58Lamp Supply (DPS-A) 60

Communication Interface (CIB / CRB) 64

3 Common: Cable Information

This chapter provides information on cables for the 1050 Modules 67

Overview 68

Analog Cables 70Remote Cables 72BCD Cables 77

4 Pumps: General Information

This chapter provides general information about the 1050 Pumps 83

Introduction 84

About this Manual 84About the Pumps 85Repair Policy 85Product Structure 86

Contents

Capillaries 87Specifications 88Service Handbook for 1050 Series of HPLC Modules - 09/2001 5

5 Pumps: Hardware Information

This chapter provides hardware information about the 1050 Pumps 91

Overview 93

How does the Pump Work? 95Isocratic Operation 95Gradient Operation 96

Overview of the Electronics 96Overview of the Flow Path 98Solvent Cabinet 99

Helium Degassing 99Manual Injection Valve 100Column Heater 100

Multi Channel Gradient Valve (MCGV) 102Metering Drive Assembly 103Pump Head Assembly 104Continuous Seal Wash 105Active Inlet Valve 107Outlet Ball Valve 108Frit Adapter Assembly 109Purge Valve 110High Pressure Damper 111Column Holder 112

Contents

6 Pumps: Electronic Information

This chapter provides electronic information about the 1050 6 Service Handbook for 1050 Series of HPLC Modules - 09/2001

Pumps 113

Overview 115Pump Drive Control Board (PDC2) 118Relative A/D Converter Board (RAD) 123Firmware Board (SFW) 127HRI Board - Heater Isocratic Board 128Heater Quaternary Board (HRQ) 131High Pressure Transducer Board (HPT) 134Connector Board (CON) 136Pump Motherboard (HPS) 138

7 Pumps: Diagnostic Information

This chapter provides information on error messages and di-agnostic features of the 1050 Pumps 143

How to use the Diagnostic Test Functions 145Pump Pressure Ripple 146Flow (Pressure) Tests 147

Prerequisites for the Pressure Tests 148Normal Pressure Test 149The Modified Pressure Test 150Flow Test Method 151Flow Test Method - Firmware Rev. 1.0 152Flow Test Method - Firmware Rev. 3.0 and above 157

Gradient Test Method 159Prerequisites for the Gradient Test Method 159Running the Gradient Test Method 159

Contents

Error Messages 162

Selftest 163Service Handbook for 1050 Series of HPLC Modules - 09/2001 7

ROM/RAM Test 163

Panic Error / Bus Error Address Error 164Common 1050 Error Messages 165Pump Initialization Error Messages 167Normal Operation Error Messages 171Column Heater Error Messages 175Online Monitor Messages 177

Troubleshooting Hints 180

Standard Pressure Tests with different Solvents 181Modified Pressure Tests 181Pressure Tests - Firmware Revision 1.0 182Pressure Tests - Firmware Revision 3.0 and above 184

Pressure Tests when the Pump is broken 185Pressure Tests - Leak at Piston Seal 1 186Pressure Tests - Leak at Piston Seal 2 189Pressure Tests - Defective Piston 1 192Pressure Tests - Defective Piston 2 196Pressure Tests - Defective Active Inlet Valve 200

8 Pumps: Maintenance Information

This chapter provides provide procedures for service and main-tenance of the 1050 Pumps 203

Solvent Cabinet and Column Heater 205Replacing the Heat Exchanger 205Replacing the Cable Assembly 206

Replacing the Active Inlet Valve 207

Contents

Replacing the Outlet Ball Valve 209Maintaining the Frit Adapter Assembly 2108 Service Handbook for 1050 Series of HPLC Modules - 09/2001

Maintaining the Purge Valve 211

Maintaining the Pump Head Assembly 212

Procedure 1: Pump Head with old Plunger Housing 213Stage 1: Removing the Pump Head Assembly 213Stage 2: Disassembling the Pump Head assembly 213Stage 3: Replacing the Seals 214Stage 4: Disassembling the Plunger Housing 215Stage 5: Reassembling the Plunger Housing 216Stage 6: Reassembling the Pump Head Assembly 217Stage 7: Mounting the Pump Head Assembly 217

Procedure 2: Pump Head with new Plunger Housing 218Stage 1: Removing the Pump Head Assembly 218Stage 2: Disassembling the Pump Head Assembly 218Stage 3: Replacing the Seals 219Stage 4: Reassembling the Pump Head Assembly 220Stage 5: Mounting the Pump Head Assembly 220

Continuous Seal Wash Option 221Replacing the Fan 222Removing the Metering Drive Assembly 223

9 Pumps: Parts Information

This chapter provides information on parts of the 1050 Pumps 225

Electronic Boards 227Complete List of Ti-Parts 229Solvent Cabinet 230

Contents

Solvent Cabinet with Helium Degassing 232Solvent Cabinet with Column Heater and Manual Injection Service Handbook for 1050 Series of HPLC Modules - 09/2001 9

Valve 234Overall Diagram 236Hydraulic Flow Path 240Metering Drive Assembly 243Pump Head Assembly (old version) 244Pump Head Assembly (new version) 245Pump Head Assembly with Seal Wash 246Active Inlet Valve 248Outlet Ball Valve 249Frit Adapter Assembly 250Purge Valve Assembly 251Column Holder Assembly 252Special Tools 253

10Pumps: Additional Information

This chapter provides additional information about the 1050 Pumps 255

Product History 257Firmware History 259

Revision 1.0 259Revision 3.0 259Revision 3.1 260Revision 3.2 260

How does the On-line Monitor work 261Normal Operation 262M2 Gas Bubble 263M4 Leak at first Piston 265

Contents

M6 Valve Backflow 266M8 Outlet Valve Problem 26710 Service Handbook for 1050 Series of HPLC Modules - 09/2001

If You Need Operational Hints 268Helium Degassing Principle 269Helium Regulators 269Bottle Head Assembly 270Isocratic Pumps 270Pump Head Assembly 271PDC Board 271PDC2 Board 271HRQ Board 272GVD Board 272Wear Retainer 272Outlet Ball Valve 273Flow Test Method 273Method loading 273Flow Gradients 273Manual Injection Valve 273Metering Drive Repairs 274Troubleshooting E27 Errors (Max Motor Drive Power Exceeded) 275Piston with Conical Holder 276Ghost Leak messages 276PANIC Errors 276

11Sampler: General Information

This chapter provides general information about the 1050 Autosampler 281

About this Manual 282About the Autosampler 282Repair Policy 283

Contents

Product Structure 283Capillaries 284Service Handbook for 1050 Series of HPLC Modules - 09/2001 11

Specifications 285

12Sampler: Hardware Information

This chapter provides hardware information about the 1050 Autosampler 287

Overview 289

Solvent Flow Path 290How Does The Autosampler Work? 291The Injection Sequence 293What happens when the 18596L/M Sample Tray is connected? 294Overview of the Electronics 295Sampling Unit 297Metering Drive 299Analytical Head Assembly 300High Pressure Switching Valve 301Pneumatic Assembly 302

Actuator Air Solenoids 303

Additional 100 Sample Capacity 304

13Sampler: Electronic Information

This chapter provides electronic information about the 1050 Autosampler 307

Overview 309

Contents

Max Tray Drive Board (MTD) 312Needle Mini Tray Drive Board (NMD) 31612 Service Handbook for 1050 Series of HPLC Modules - 09/2001

Valve Metering Drive Board (VMD) 320Firmware Board (FIM) 324Autosampler Motherboard (ALM) 325Extender Test Board (ET) 330

14Sampler: Diagnostic Information

This chapter provides information on error messages and di-agnostic features of the 1050 Autosampler 333

Single Steps 335Entering the Test Functions 335

Single Steps For The 21 Sample Tray 336Single Steps for the 100 Sample Tray 338

Entering the Additional Single Steps 338

18596L/M Sample Tray Diagnostic Mode 340Entering Diagnostic Mode 340Z Test (Gripper Assembly) 340R Test (radial arm movement) 341Theta Test (angular movement) 341

Error Messages 342Selftest 343Panic Error 343

Common 1050 Error Messages 344Error Messages for Firmware Revision 4.0 and greater 346Injector Program Error Messages 350Normal Operation Messages for Firmware Revision 3.1 and below 351Events Messages 354

Contents

18596L/M Vial Tray 355Service Handbook for 1050 Series of HPLC Modules - 09/2001 13

15Sampler: Maintenance Information

This chapter provides provide procedures for service and main-tenance of the 1050 Autosampler 357

Sampling Unit 359Stage 1: Removing the Sampling Unit 359Stage 2: Removing the Needle 360Stage 3: Installation of the Needle 361Stage 4: Removing the Seat Capillary 361Stage 5: Disassembling the Needle Arm 362Stage 6: Reassembling the Needle Arm 362Stage 7: Disassembling the Tray Mechanic 363

Metering Device 365Removing the Metering Device 365Removing the Gear Belt 365Analytical Head Assembly 366Procedure 1: Analytical Head Assembly with old Adapter Housing 366Procedure 2: Analytical Head with new Adapter Housing 369Reassembling the Metering Device 371

High Pressure Switching Valve 372Stage 1: Removing 372Stage 2: Disassembling 372

Adjust the Sensors 374Service Only Level 374Sensors of the Sampling Unit 376Sensor of the High Pressure Switching Valve 379Metering Device Home Sensor 380

Contents

16Sampler: Parts Information

This chapter provides information on parts of the 1050 14 Service Handbook for 1050 Series of HPLC Modules - 09/2001

Autosampler 381

Electronic Boards and Fuses 383Electronic Boards 383Fuses 383

Complete List of Ti-Parts 384Overall Diagram 79855A/B 385Hydraulic Flow Path 389Sampling Unit 391

Arm Assembly Spare Parts 395

Metering Drive and Analytical Head 396Metering Drive 396Analytical Head (Old Version) 396

High Pressure Switching Valve 399Pneumatic Valve Assembly 401

17Sampler: Additional Information

This chapter provides additional information about the 1050 Autosampler 403

Product History 405Firmware Revisions 406

Firmware Revision 1.0 406Firmware Revision 2.0 406Firmware Revision 2.1 407Firmware Revision 3.0 408Firmware Revision 3.1 409Firmware Revision 4.0 410

Contents

Firmware Revision 4.1 411Firmware Revision 4.2 411Service Handbook for 1050 Series of HPLC Modules - 09/2001 15

If you update the firmware to revision 4.0 and greater 412If you add a 100 vial tray to the autosampler 412If you have Intermittant E17: Needle cannot move out of vial 413If you have to update the autosampler with a fan 413If the fan in the autosampler does not work properly 413If the needle lifts the vial out of the tray 414If the injections are not reproducible (grooved needle) 414

18DAD/MWD: General Information

This chapter provides general information about the 1050 Di-ode Array and Multiple Wavelength Detectors 419

About the Detector 420General 420Repair Policy 420Identification 421Compatibility 421

DAD Differences 422Added features 422Removed features from local keyboard 422Restrictions of user interface 423Compatibility 423Local User Interface 424Workstation Interface 426Test Functions 428Options 428

Specifications DAD/MWD 429

Contents

19DAD/MWD: Hardware Information

This chapter provides hardware information about the 050 Di-16 Service Handbook for 1050 Series of HPLC Modules - 09/2001

ode Array and Multiple Wavelength Detectors 433

Overview: Optical System 435Overview: Electronics 436Optical Unit 438

Flow Cell Assemblies 439Slit Assembly 441Deuterium Lamp Assembly 442Heat Exchanger Assembly 444Shutter Assembly 445Leak Sensor Assembly 446

Fans 447

20DAD/MWD: Electronic Information

This chapter provides electronic information about the 1050 Diode Array and Multiple Wavelength Detectors 449

Overview 451Array Signal Conversion Board (ASC) 454Data Acquisition Board (AQB) 459Firmware Board (FIM) 463Common Main Processor Board (CMP) 464

Remote Control 466

Communication Interface (CRB) 467Digital to Analog Conversion Board (DAC) 468Fluorescent Indicator Module (FIP) 472Motherboard (LUM) 473LPC Board 477

Contents

Power Supply (DPS-A) 478Service Handbook for 1050 Series of HPLC Modules - 09/2001 17

21DAD/MWD: Diagnostic & Troubleshooting Information

This chapter provides information on error messages and di-agnostic features of the 1050 Diode Array and Multiple Wave-length Detectors 483

STATUS Information 485Status Modes 485Status LEDs 486Warnings 486

Error Messages 487Selftest 487

Common 1050 Error Messages 4881050 DAD/WMD Error Messages 490Diagnostic Features 493

Entering the Test Functions 493

Measure Intensity Profile 494Lamp Intensity Test 496Measure Holmium Spectrum 498D/A Converter Test 500Electronic Noise Test 502Check of Wavelength Calibration 504ASC Test 505Shutter Position 506ROM/RAM/DISPLAY Tests 507Using the Built-in Test Chromatogram 508

How to print the DAD Profiles 510

Contents

22DAD/MWD: Maintenance Information

This chapter provides provide procedures for service and main-18 Service Handbook for 1050 Series of HPLC Modules - 09/2001

tenance of the 1050 Diode Array and Multiple Wavelength Detectors 513

Tools Needed 514Warnings and Notes 515

Removing the Optical Unit 516Flow Cell Maintenance 517

Flushing Procedure 517Replacements on Standard Flow Cells 518Replacements on High Pressure Flow Cells 520

Lamp House Window Maintenance 522Removing the Quartz Window 522

Replacing the Achromat 524Replacing Fans 525Replacement of Shutter or LPC Board 526Replacement of Leak Sensor 527Upgrade to from 79854A MWD to G1306A DAD 528Upgrade MWD with Series II Optical 529Verifying the Performance 530

Specifications 530What You Need 530Preparations 530Scaling Factors 532

23DAD/MWD: Parts Information

This chapter provides information on parts of the 1050 Diode Array and Multiple Wavelength Detectors 533

Contents

Overall Diagram 535Optical Unit 538Service Handbook for 1050 Series of HPLC Modules - 09/2001 19

Heat Exchanger and Flow Cell 540Flow Cell Parts (STD-SST) 541High Pressure Flow Cell Parts (HP-STD-SST) 542High Pressure Flow Cell Parts (HP-Micro-SST) 543Cell Repair Kits 544Lamp Housing 545Upgrade Parts MWD to DAD 546Upgrade Parts MWD to Series II Optical 547List of Accessories 548

24DAD/MWD: Additional Information

This chapter provides additional information about the 1050 Diode Array and Multiple Wavelength Detectors 549

Product History 551Hardware Changes 551

Firmware Changes 552Known Problems 553

Panic Errors 553

25VWD: General Information

This chapter provides general information about the 1050 Variable Wavelength Detectors 559

About the Detector 561Versions vs. Support Periods (EOS) 561

Contents

79853A 56179853C 56120 Service Handbook for 1050 Series of HPLC Modules - 09/2001

Repair Policy 562Specifications 563

26VWD: Hardware Information

This chapter provides hardware information about the 1050 Variable Wavelength Detectors 565

Overview 566Optical System Overview 568Leak Interface Assembly 569Leak Sensor Assembly 570Fan Assemblies 571Optical Unit 572

Flow Cells 573Deuterium Lamp 576Photodiodes Assemblies 579Filter Assembly 580Grating Assembly and Motor 581Mirrors 582Slit Assemblies 582Beam Splitter 582

Enhanced Optical Unit (D) 583

27VWD: Electronic Information

This chapter provides electronic information about the 1050 Variable Wavelength Detectors 585

Contents

Location of Electronic Assemblies 587Interconnection Diagram 589Service Handbook for 1050 Series of HPLC Modules - 09/2001 21

Detector Controller Board (DCB) 590Digital Section 592Analog Sections 594

Power Supply (DPS-A) 601Keyboard 602

Keyboard Electronics (KDI / VFD) 603

Pre-Amplifier Boards 605Power Supply Connection Board (PSC) 606GPIB Communication Interface 607

GPIB Firmware Revisions 608

28VWD: Diagnostic & Troubleshooting Information

This chapter provides information on error messages and di-agnostic features of the 1050 Variable Wavelength Detectors 609

Self Diagnosis 611During Power On 611During Normal Operation 611

Error Messages Before Lamp Ignition 612At Power ON 612

Error Messages After Lamp Ignition 616Error Messages During Normal Operation 617Error Messages During Use of Control Functions 619User Control Functions 620Service Control Functions 622

Entering the Service Mode 622Zero Order Calibration 624

Contents

Wavelength Calibration 626Wavelength Calibration Check 62822 Service Handbook for 1050 Series of HPLC Modules - 09/2001

SET WL Parameter 629Fix Signal 631Leak Sensor Voltage 634Voltage Test 635ADC Noise 636Grating Photo Sensor 637Filter Photo Sensor 638Remote Test 639Filter Check 640Zero Order Test 641DAC Test 642Pre-amplifier Gain 644EEROM Test 645DAC Calibration 646Wavelength Compensation 647

29VWD: Maintenance Information

This chapter provides provide procedures for service and main-tenance of the 1050 Variable Wavelength Detectors 649

Warnings 650Securing for Transport 651Replacement of Deuterium Lamp 652

Step 1: Replacement 652Step 2: 0th Order Calibration 653Step 3: WL CALIBRATION 654

Flow Cell Maintenance 655Flow Cell Maintenance Kits 655Replacing Cell Parts 655

Contents

Flushing Procedure 656Leak Test 657Service Handbook for 1050 Series of HPLC Modules - 09/2001 23

Using the Cuvette Holder 658Replacing DCB Board and Firmware 661

DCB Board 661DCB Firmware 661

Replacing Display Boards 662Replacing the Leak Interface 663

Leak Sensor Assembly 663Leak Interface 664

Replacements in the Optical Unit 665Removing the Optical Unit 666Replacing the PSC Board 667Replacing Pre-amplifiers or Photodiodes 667Replacing Grating Assembly Parts 668Replacing Filter Assembly Parts 670Replacing Mirrors, Beamsplitter and Slits 670

Optical Alignment Procedures 671Procedure 1: Simple Alignment 671Procedure 2: Sample Beam Alignment 672Procedure 3: Reference Beam Alignment 674

Cleaning of Optical Unit Parts 675Upgrade to GPIB 676Performance Verification 677

What you need 677Preparations 677Starting a run 678Scaling Factors 679

30VWD: Parts Information

This chapter provides information on parts of the 1050 Vari-

Contents

able Wavelength Detectors 68124 Service Handbook for 1050 Series of HPLC Modules - 09/2001

Overall Diagram 683Front Panel Parts 686

Leak Interface 686Font Panel 687

Optical Unit C 688Optical Unit C Inner Parts Top 689Optical Unit C Inner Parts Bottom 690

Grating Assembly 691Filter Assembly 692Standard Flow Cell C (SST/Ti) 693Semi-Micro Flow Cell (SST) 695High Pressure Flow Cell (SST) 696Ultra High Pressure Flow Cell (SST) 697Preparative Flow Cell (Ti) 698Cuvette Holder 700Accessories 701Screws 702

31VWD: Enhanced Optical Unit Information

This chapter provides information about the enhanced optical unit D 705

Compatibility 706Support of Previous Optical Units 706Introduction 707Support Considerations 708

Prefix Change 708Identification 708

Contents

Compatibility Matrix 708

Part Numbers for Enhanced D Optical Unit 709Service Handbook for 1050 Series of HPLC Modules - 09/2001 25

Standard Flow Cell D Repair Parts 710Repair and Mainenance 711

Tools required: 711Pre-requisites: 711

Additional Information 712Replacements and Calibrations 712Installing the Test Slit 713Replacing Mirror #1 Assembly 715Replacing Mirror #3 or #4 Assembly 716Replacing the Grating or Grating Motor 718Replacing the Beam Splitter 720Cleaning or Replacing the Lens 721Unlocking the Reference Aperture 723Optimizing the Reference Readings 725Installing the Standard Slit 726

32VWD: Additional Information

This chapter provides additional information about the 1050 Variable Wavelength Detectors 727

Product History 729Prefix Changes 729

DCB ROM Firmware Revisions 731GPIB ROM Firmware Revisions 733Hardware Changes and Service Notes 734

Modified Pre-Amplifier Gain 734Important Service Note 734

Contents26 Service Handbook for 1050 Series of HPLC Modules - 09/2001


Service Handbook - Common Information




Part No. NONE

11/2001

Printed in Germany

Warranty




IMPORTANT NOTE

This version of the 1050 service manual includes all sections from the 01050-90102 edition 4 (1995) and G1306-90102 edition 2 (May 1994). It merges both sections, the MWD and the DAD.

The series I opticals information (79854A MWD) information has been removed (product went out of support during 2000).



11 Common: General Information

This chapter provides general information about the 1050 Series of HPLC Modules

Common: General InformationSafety Information

Safety Information30 Service Handbook for 1050 Series of HPLC Modules - 11/2001

The following general safety precautions must be observed during all phases of operation, service, and repair of this instrument. Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design, manufacture, and intended use of the instrument. Agilent Technologies assumes no liability for the customers failure to comply with these requirements.

General

This is a Safety Class I instrument (provided with terminal for protective earthing) and has been manufactured and tested according to international safety standards.

Operation

Before applying power, comply with the installation section. Additionally the following must be observed.

Do not remove instrument covers when operating. Before the instrument is switched on, all protective earth terminals, extension cords, auto-transformers, and devices connected to it must be connected to a protective earth via a ground socket. Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury. Whenever it is likely that the protection has been impaired, the instrument must be made inoperative and be secured against any intended operation.

Make sure that only fuses with the required rated current and of the specified type (normal blow, time delay, and so on.) are used for replacement. The use of repaired fuses and the short-circuiting of fuseholders must be avoided.

Some adjustments described in the manual, are made with power supplied to the instrument, and protective covers removed. Energy available at many points may, if contacted, result in personal injury.

Any adjustment, maintenance, and repair of the opened instrument under voltage should be avoided as much as possible. When inevitable, this should be carried out by a skilled person who is aware of the hazard involved. Do not attempt internal service or adjustment unless another person, capable of

Common: General InformationSafety Informationrendering first aid and resuscitation, is present. Do not replace components with power cable connected.

Do not operate the instrument in the presence of flammable gases or fumes. Operation of any electrical instrument in such an environment constitutes a definite safety hazard.

Do not install substitute parts or make any unauthorized modification to the instrument.

Capacitors inside the instrument may still be charged, even though the instrument has been disconnected from its source of supply. Dangerous voltages, capable of causing serious personal injury, are present in this instrument. Use extreme caution when handling, testing and adjusting.

When working with solvents please observe appropriate safety procedures (for example, goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet by the solvent vendor, especially when toxic or hazardous solvents are used.Service Handbook for 1050 Series of HPLC Modules - 11/2001 31

Common: General InformationSafety Information

Safety Symbols

Table 1 shows safety symbols that are used on the instrument and in the manuals.

WA R

CA U

Table32 Service Handbook for 1050 Series of HPLC Modules - 11/2001

N I N G A warning alerts you to situations that could cause physical injury or damage to the equipment. Do not proceed beyond a warning until you have fully understood and met the indicated conditions.

TI O N A caution alerts you to situations that could cause a possible loss of data. Do not proceed beyond a caution until you have fully understood and met the indicated conditions.

1 Safety Symbols

Symbol Description

The apparatus is marked with this symbol when the user should refer to the instruction manual in order to prevent risk of harm to the operator and protect the apparatus against damage.

Indicates dangerous voltages.

Indicates a protected ground terminal.

Eye damage may result from directly viewing the light produced by the deuterium lamp used in this product. Always turn off the deuterium lamp before opening the metal lamp door on the side of the instrument.

Common: General InformationRadio Interference

Radio InterferenceService Handbook for 1050 Series of HPLC Modules - 11/2001 33

Manufacturers Declaration

This is to certify that this equipment is in accordance with the Radio Interference Requirements of Directive FTZ 1046/1984. The German Bundespost was notified that this equipment was put into circulation, the right to check the series for compliance with the requirements was granted.

Test and Measurement

If test and measurement equipment is operated with equipment unscreened cables and/or used for measurements on open set-ups, the user has to assure that under operating conditions the radio interference limits are still met within the premises.

Sound Emission

Manufacturers Declaration

This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991.

This product has a sound pressure emission (at the operator position)< 70 dB.

Sound Pressure Lp < 70 dB (A)

At Operator Position

Normal Operation

According to ISO 7779:1988/EN 27779/1991 (Type Test)

Common: General InformationUV-Radiation

UV-Radiation

Table


Emissions of ultraviolet radiation (200-315 nm) from this product is limited such that radiant exposure incident upon the unprotected skin or eye of operator or service personnel is limited to the following TLVs (Threshold Limit Values) according to the American Conference of Governmental Industrial Hygienists:

Typically the radiation values are much smaller than these limits:

2 UV-Radiation Limits

Exposure/day Effective Irradiance

8 hours 0.1 W/cm2

10 minutes 5.0 W/cm2

3 UV-Radiation Typical Values

Position Effective Irradiance

Lamp installed, 50-cm distance average 0.016 W/cm2

Lamp installed, 50-cm distance maximum 0.14 W/cm2

Common: General InformationSolvent Information

Solvent InformationService Handbook for 1050 Series of HPLC Modules - 11/2001 35

Observe the following recommendations on the use of solvents.

Flow Cell

Long term operation at pH > 11 should be avoided. Never leave strongly alkaline solutions in the flow cell without flow.

Solvents

Always filter solvents through 0.4 m filters, small particles can permanently block the capillaries. Avoid the use of the following steel-corrosive solvents:

Solutions of alkali halides and their respective acids (for example, lithium iodide, potassium chloride, and so on).

High concentrations of inorganic acids like sulfuric acid, especially at higher temperatures (replace, if your chromatography method allows, by phosphoric acid or phosphate buffer which are less corrosive against stainless steel).

Halogenated solvents or mixtures which form radicals and/or acids, for example:

2CHCl3 + O2 2COCl2 + 2HCl

This reaction, in which stainless steel probably acts as a catalyst, occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol.

Chromatographic grade ethers, which can contain peroxides (for example, THF, dioxane, di-isopropylether) such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides.

Solutions of organic acids (acetic acid, formic acid, and so on) in organic solvents. For example, a 1-% solution of acetic acid in methanol may attack steel.

Mixtures of carbon tetrachloride with 2-propanol or THF dissolve stainless steel.

Common: General Information1050 Introduction

1050 Introduction


The Modules Overview

1050 is a series of HPLC products based on a modular concept. The necessary functions are broken down into independent stand-alone modules with standardized external design hydraulic- and external interfaces. Following modules will be available at introduction:

4 1050 Modules

Module Product Number

1050 Isocratic Pump 79851A

1050 Quaternary Pump 79852A

1050 Quaternary Pump (bio compatible) 79852B

1050 Variable Wavelength Detector 79853C

1050 Multiple Wavelength Detector 79854A

1050 Diode Array Detector G1306A

1050 Autosampler 79855A

1050 Autosampler (bio compatible) 79855B


Figure 1 1050 ModulesService Handbook for 1050 Series of HPLC Modules - 11/2001 37


1050 Identification

Each module is identified by a 5 digit product number and a 10 unit serial number on a label attached to the wall inside the module. The first four digits of the serial number are the serial prefix. The letter identifies the country of 38 Service Handbook for 1050 Series of HPLC Modules - 11/2001

origin. The last five digits are an identification number unique to each module. Any changes to the modules will be covered initially by Service Notes. They will be sent out to all Service personnel prior to implementation of the change to the instrument. With every reprint these changes will be incorporated into the documentation.

Repair Policy

Major mechanical and electrical assemblies inside the 1050 modules will be repaired on an assembly-exchange level. All other items have to be repaired on board/component level. Repair procedures are given in the respective sections of this manual (refer to Table of Contents). Assemblies can be set up to the Blue Stripe Exchange system or can be removed. If in doubt contact Waldbronn Product Support (Europe/ICON) or Little Falls Product Support (USA/Canada).

22 Common: Electronic Information

This chapter provides common electronic information about the 1050 Series of HPLC Modules

Common: Electronic InformationThis chapter gives information about the common electronics used in more than one of the 1050 Series of modules:

Overview

Common Main Processor (CMP)

Remote Control

Firmware Boards (FIM, SFW)

Fluorescent Indicator Module (FIP)

External Contacts

Poweer Supplies (DPS-B, DPS-A)

Communication Interfaces (CIB, CRB)40 Service Handbook for 1050 Series of HPLC Modules - 11/2001

Common: Electronic InformationOverview

Overview

TableService Handbook for 1050 Series of HPLC Modules - 11/2001 41

Some of the electronic boards are used in more than one 1050 module.

The following table shows common electronic assemblies:

5 Common Electronic Boards

Description Modules Part Number Exchange

Power Supply (DPS-B) pump, sampler 5061-3374 01050-69374

Power Supply (DPS-A) MWD, DAD, VWD 5061-3375 01050-69375

Common Main Processor (CMP) pump, sampler, MWD, DAD 5061-3380 01050-69580

Display Interface Board (FIP) pump, sampler, MWD, DAD 5061-3376 no

Communication Interface (CIB) pump, sampler 5061-3382 no

Communication Interface (CRB) MWD, DAD 5062-2482 no

Common: Electronic InformationCommon Main Processor Board (CMP)

Common Main Processor Board (CMP)


Repair Level: Board

Common 1050 Functions

display handling

keyboard polling

remote control input and output

leak sensing

option interfacing

time programming

method storage

module configuration

memory switching

32 kbyte RAM with battery back-up for parameter storage.

6 Part Numbers for CMP Board

Item Part Number

CMP Board (Exchange) 01050-69580

CMP Board (NEW) 5061-3380


Figure 2 Blockdiagram CMPService Handbook for 1050 Series of HPLC Modules - 11/2001 43


According to the above functions the main processor board contains some basic hardware which is common to all 1050 modules:

68008 main processor running at 8 MHz;

64 kByte RAM (32 kbyte RAM with battery back-up for parameter storage. 44 Service Handbook for 1050 Series of HPLC Modules - 11/2001

The data will be lost when CMP is removed from the slot);

interrupt logic for system communication;

3 channel software controlled timer;

interface to keyboard/display module;

remote I/O hardware;

leak sensor electronics;

interface to backplane bus;

watchdog hardware.

Firmware is not part of this board, because parts of the main processors software are module specific. The main processor firmware will be located on the personality module (AQB-, RAD, VMD-Board) or on an optional board.

CMP Details

Interrupt system

There are one non-maskable interrupt six high priority hardware interrupt lines and seven low priority mail interrupt lines. The non-maskable interrupt is connected to the powerfail line of the power supplies (DPS-A/B).

The high priority interrupt lines are: One from timer 6840 for hardware synchronization and five from remaining slots (these lines are disabled by SOK- = HIGH (system not ok).

The low priority interrupt lines are: Five lines from all slots except power supply used for communication with local processors via dual port RAMs and two lines for CMP controlled software interrupts.

Watchdog timer

(Test for CPU hang-up) This circuitry is software retriggerable and is disabled during CPU initialization. In case of CPU hang-up SOK line is set the CPU is halted and the remote line shutdown is set.


LED on board

There is a RED LED on the board which is the output of the watchdog circuit. It is ON during initialization and when the processor has a hang-up (LD 101).Service Handbook for 1050 Series of HPLC Modules - 11/2001 45

Programmable timer

It includes 3 independent timers:

Timer 1 is connected to the backplane bus its free for module special use.

Timer 2 is used as software timer for the CMP.

Timer 3 is used to generate the BUS ERROR signal.

Reset system

A harware reset is performed

at power

manually by on-board switch

Hardware reset will reset all devices connected to the bus but main processor can reset these devices by software too.

Reset for display unit

The latch for the status LEDs and the brigthness control will not be reseted by power on or by software reset. The alphanumeric display is reseted at power on.

I/O

Two remote connectors are at the rear panel. They provide start, stop, not ready, shutdown, prepare-run and power on signals. The remote lines are input and output and are decoupled for EMC. The shutdown line is set by hardware in the case of leak or CPU hang-up.

System control

The POK (peripheral OK) is driven from all devices. The SOK- (system OK)

is outputted from main processor to all devices;

is hardware and software controlled

disables/enables all devices by main software;

disables all devices if main processor watchdog becomes active (main processor hang-up);


All devices are enabled after initialization. Bus control After bus request the main processor will pass bus control to the requesting external controller. The local main processor areas including I/O are accessible too.

This may be a feature for diagnostics or if data rate is increasing to much 46 Service Handbook for 1050 Series of HPLC Modules - 11/2001

with later options. For this second case an external fast transfer hardware (for example DMA device) could do the transfers after set-up by the main processor.

Leak sensing

The leak detection circuit is located on the CMP board and checks continuously for presence and leak conditions. If the sensor is missing (defect) or in leak condition the PTC is cooled down the error message appears. When the module is turned on the leak message is disabled for some time to allow the sensor to reach its working range.

Actions:

Check for leak.

Check connector of the sensor.

Check resistance of leak sensor.

Change leak sensor.

Change CMP board.

Change FIM board.

Working condition of the PTC

Normal: about 75C 400...500 Ohm

Error: below 55C about 150 Ohm


Remote Control

The CMP board provides two remote connectors.

Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements.

FigurService Handbook for 1050 Series of HPLC Modules - 11/2001 47

When 1050 System is started from the autosampler the following signals can be measured at the remote lines. The START REQUEST signal is only available when the autosampler was started from any other module (remote configuration set to HPSystem).

e 3 Remote Control Analysis


For the 1050 Series of HPLC Modules the subminiatur D connector is used. Each module provides two remote connectors which are both parallel and inputs/outputs (wired-or technique).

To provide maximum safety within a distributed analysis system one line is 48 Service Handbook for 1050 Series of HPLC Modules - 11/2001

dedicated to SHUT DOWN the systems critical parts in case any module detects a serious problem.

To detect whether all participating modules are switched on or properly powered one line is defined to summarize the POWER ON state of all connected modules.

Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective lines. In addition PREPARE and START REQUEST may be issued.

Signal description

The signal level are defined as standard TTL levels (0 V is logic true, + 5 V is logic false). The remote lines can be input or output (wired or technique).

Fan-out is 10

Input Load 2 kOhm against + 5 V

Outputs are open collector type

SHUT DOWN (L) System has serious problem (e.g. leak: stops pump). Receiver is any module capable to reduce safety risk.

POWER ON (H) All modules connected tosystem are switched on. Receiver is any module relying on operation of others.

READY (H) System is ready for next analysis. Receiver is any sequence controller.

PREPARE (L) Request to prepare for analysis (e.g. calibration detector lamp on). Receiver is any module performing preanalysis activities.

START REQUEST (L) Request to start injection cycle (e.g. by start key on any module). Receiver is the autosampler.

START (L) Request to start run / timetable. Receiver is any module performing runtime controlled activities.

STOP (L) Request to reach system ready state as soon as possible (e.g. stop run abort or finish and stop injection). Receiver is any module performing runtime controlled activities.


The REMOTE Connector

To help you make the correct connections the signals carried on each pin are listed in the table below (the colors refer to wires of remote cable 01046-60201).

Figur


e 4 APG Remote Connector

Remote Configuration

The 1050 Series provides three remote configurations:

7 Remote Signals

Pin Signal Active Color

1 Digital ground white

2 Prepare run LOW brown

3 Start LOW gray

4 Shut down LOW blue

5 Reserved pink

6 Power ON HIGH yellow

7 Ready HIGH red

8 Stop LOW green

9 Start request LOW black

HPsystem Start of automatic operation from any modules start key. Start request is outputted.

GLOBAL Synchronized start of several modules for a single run.Start / Stop is outputted.

LOCAL Single modules start. No pulses outputted.


Figure 5 Table of line definition50 Service Handbook for 1050 Series of HPLC Modules - 11/2001

Notes

Y1 is done by balance key of MWD only.

Y2 BALANCE on detectors is performed.

Y3 is not used in the module.

The remote line SHUT DOWN will always be active.

The remote line POWER ON will not be processed.


Figure 6 Schematic of Remote Control

N OTService Handbook for 1050 Series of HPLC Modules - 11/2001 51

E Above schematic is for Pump, Autosampler, MWD and DAD.

The signal level are defined as standard TTL levels

(0 V is logic true, +5 V is logic false).

The remote lines can be input or output (wired or technique).

Fan-out is 10

Input Load >=2.2 kOhm against + 5 V

Outputs are open collector type


Figure 7 Board Layout CMP52 Service Handbook for 1050 Series of HPLC Modules - 11/2001

Common: Electronic InformationFirmware Board (FIM)

Firmware Board (FIM)


Repair Level: Exchange Board

Firmware Description

Figure 3-7 shows the firmware structure for the 1050 Series of modules (pump, autosampler, multiple wavelength detector and diode array detector). As many as possible tasks use the same core firmware and only special routines for each module are developed seperate (control of the hardware sensors motors and so on). This common structure gives maximum flexibility for later development of similar products.

It is obvious that also in the common firmware different commands display contents method parameters and so on. appear (Dialog, Method Handler, Parameter Handler). But nevertheless the structure is the same. In each part of the firmware there exist tables which hold the module specific commands parameters and so on, which are all handled under the same conditions.

The firmware works with a foreground background mode. All time critical tasks (timetable execution, sensor and motor information) are working in the foreground mode and have highest priority. All other tasks share the remaining time in the background. If there are no tasks running the processor goes into the idle state.

The firmware per module has approxmiately 300 kByte, where 170 kByte is Common and 130 kByte module specific).

8 Part Numbers for Firmware Boards

Item Part Number Exchange

for Pumps (79851/2A/B) on RAD Board 01018-66518 no

for Autosamplers (79855A/B) on VMD Board 01078-66504 no

for Multiple Wavelength Detectors (79854A) on AQB Board

01048-66504 no

for Diode ArrayDetectors (G1306A) on AQB Board G1306-66524 no

Common: Electronic InformationFirmware Board (FIM)

The firmware is located on the module specific firmware board which is piggy back on the personality board of each module (AQB-, RAD- or VMD-board) and can be exchanged easily.

Figure 8 Firmware Structure54 Service Handbook for 1050 Series of HPLC Modules - 11/2001

Common: Electronic InformationFluorescent Indicator Module (FIP)

Fluorescent Indicator Module (FIP)

Figur


Repair Level: Board or Fuse ICP1

The FIP module is located behind the keyboard module of pump, autosampler and multiple wavelength detector.

The function of the FIP module is to provide an interface between a host system and the user. Messages can be displayed with up to 32 characters (2 lines x 16 characters/line). A matrix keyboard is scanned for numeric or special function input and status information is displayed through 4 LEDs. The characters are displayed in a 5 x 7 dot matrix.

In case of a dark display, check the on board fuse ICP1 (1 A) which is soldered in close to the connector P1/P2.

e 9 Board Layout FIP

9 Part Numbers for FIP Board

Item Part Number used for

FIP Board 5061-3376 pumps, autosampler, MWD amd DAD

Fuse 1 A 2110-0099

Common: Electronic InformationExternal Contacts

External Contacts

Figur


The personality boards of the 1050 modules (MWD/DAD: AQB, Pumps: RAD and Autosampler: VMD) have two external conacts at the rear.

1 contact without supply (contact closure) max. 30 V/250 mA (fused with 250 mA)

1 contact with internal 24 V supply (max. 250 mA output with fuse)

The schematic for all three boards (AQB, NMD and RAD) is in general the same. Only the values of the components vary from board to board due to internal specifications.

e 10 External Contacts

10 Components of External Contacts

Components AQB RAD VMD

L1, L2, L3, L4 4.7 H 10 H 1 H

C1, C2, C6, C7 100 nF 1 nF

C3, C4, C8, C9 10 nF 10 nF 10 nF

C5, C10 1 nF

Fuse F250 mA (2110-0004)

Common: Electronic InformationPower Supply (DPS-B / DPS-A)

Power Supply (DPS-B / DPS-A)


Repair Level: Fuses and DPS-B / DPS-A

General Description

The power supply is a primary switching regulated type. It consists of two parts. the Base Supply and the Lamp Supply. The Base Supply provides outputs of +5 V, 19 V, +24 V and +36 V. In addition the Lamp Supply provides all circuits necessary for the operation of a deuterium lamp.

11 Part Numbers for LUC/LPC Board


DPS-B (Exchange) 01050-69374 Pumps and Autosamplers

DPS-B (New) 5061-3374 Pumps and Autosamplers

DPS-A (Exchange) 01050-69375 MWD, DAD, VWD

DPS-A (New) 5061-3375 MWD, DAD, VWD

Fuse for 110 V operation 3 A 2110-0003

Fuse for 220 V operation 2 A 2110-0002


Base Supply (DPS-B)

Figure 11 on page 59 shows the base part of the DPS-A. The line voltage is rectified filtered and switched with about 50 kHz by a power MOS-FET. The complete control of frequency and pulsewidth is made by the control

N OT58 Service Handbook for 1050 Series of HPLC Modules - 11/2001

board #1 containing the logic needed and the FET driver.

The isolation between the primary and the secondary part is made by opto-couplers and the switching transformer. The DC-output voltages are generated by single-phase rectifiers and LC-filtering with the additional features: The +36 V output has an separate over-voltage protection to limit the voltage to +45 V maximum. The +5 V output contains an additional analog series regulator to provide a stable output for all load conditions under different applications. The synchronization input is used in the 1050 MWD/DAD only to synchronize the switching frequency to a value of three times (54 kHz) of the diode array readout frequency. This output is not used in the other modules.

The power supply status is monitored by the processor system to detect a powerfail condition and to save all important data. The Power Supply STATUS LED (GREEN) at the rear panel shows the OK condition of the power supply.

E OK means that the pulsewidth of the switching FET is inside the allowed limits. OK does not means that all voltages at the output are present (for example a broken inductor is not detected).


Figure 11 Block Diagram DPS-B (Base Supply)Service Handbook for 1050 Series of HPLC Modules - 11/2001 59


Lamp Supply (DPS-A)

Figure 12 on page 61 and Figure 13 on page 62 show the additional circuits necessary for the deuterium lamp:

a DC output of 5.5 V for the regulated heater output (located on the

WA R60 Service Handbook for 1050 Series of HPLC Modules - 11/2001

primary board);

a regulated constant current source with selectable current of 320 mA, 360 mA or 400 mA;

a 600 V lamp ignition circuit;

a 12 V regulated output for future use.

N I N G Hazardous voltage present at the output connector with instrument power cord connected to AC line.

The main feature of this power supply is a low noise current source for the deuterium lamp. For realization a pulse-width modulated DC-DC converter (36 V input, 170 V no load output) is built-up with a switching FET and high voltage transformer. The pulse-width is regulated so that the DC-output is about 12V above the actual anode voltage of the deuterium lamp.

This design allows minimum power loss if the anode voltage varies from lamp to lamp and by aging between 65 V and 100 V. The final regulation to the selected current is made by an analog power regulator. Again the switching frequency is synchronized to 54 kHz in the 1050 MWD/DAD.


Figure 12 Block Diagram DPS-A (Lamp Supply I)Service Handbook for 1050 Series of HPLC Modules - 11/2001 61


Figure 13 Block Diagram DPS-A (Lamp Supply II)62 Service Handbook for 1050 Series of HPLC Modules - 11/2001


Lamp Ignition

To ignite the deuterium lamp a 0.5 F capacitor loaded with 600 V is discharged via a 10 kOhm resistor to the anode. These 600 V are generated by a separate winding.Service Handbook for 1050 Series of HPLC Modules - 11/2001 63

The lamp status output signal shows "OK" if the lamp current has the selected value. Otherwise an error message is generated.

The heater output made by a series regulator is in the pre-heating status 2.5 V always. After ignition a different output voltage is selected depending on the lamp type used:

In the 79853C VWD, 79854A MWD and the G1306A DAD, the heater is switched off after ignition.

The 12 V low noise output is made by a series regulator connected to the +19 V output.

Common: Electronic InformationCommunication Interface (CIB / CRB)

Communication Interface (CIB / CRB)

N OT


Repair Level: Exchange Board

E This section describes the communication interface for the 1050 Pumps (79851/2A/B), Autosamplers (79855A/B), Multiple Wavelength Detectors (79854A) and Diode Array Detector (G1306A) only.

The communication interface for the 1050 Variable Wavelength Detector (79853C) is described in the chapter of the 1050 VW Detector.

The communication interface board is necessary for the control by a Personal Computer and to connect printer or plotter devices. The communication interface board provides one GPIB and one RS-232 interface. The CRB for the 1050 MWD/DAD has a 96 kbyte runbuffer for the data/spectrum operation with the Multiple Wavelength Detector. The interface is located in Slot #2 of the module.

12 Part Numbers for CRB Board


CIB Board (NEW) 5061-3382 Pumps and Autosampler

CRB Board (NEW) 5062-2482 79854A MWD / G1306A DAD

Common: Electronic InformationCommunication Interface (CIB / CRB)

Compatibilities

Table 13 CIB/CRB CompatibilityService Handbook for 1050 Series of HPLC Modules - 11/2001 65

Firmware

To use the communication interface board it is mendatory to have the 1050 Modules equipped with the latest firmware revisions (see Table 13).

Baud rate

The board contains a baudrate generator. The baudrate is setable up to 19200 baud from the keyboard. The transmitter and receiver baudrate are independent adjustable.

RS-232 Interface

The implemented serial interface is a subset of the RS-232 standard only. It contains at

The 1050 modules are designed as DCE (data communication equipment) without hardware handshake.

Instrument CIB CRB Firmware

1050 Pump R P REV 3.1

1050 Sampler R P REV 3.1

1050 MWD C R REV 3.1

1050 DAD C R REV 1.0

R recommended configuration

P possible but not neccessary

C only for instrument control

PIN 2 RxD receive data (data input)

PIN 3 TxD transmit data (data output)

PIN 4 GND (Ground)

Common: Electronic InformationCommunication Interface (CIB / CRB)66 Service Handbook for 1050 Series of HPLC Modules - 11/2001

33 Common: Cable Information

This chapter provides information on cables for the 1050 Modules

Common: Cable Information

WA ROverview

The 1050 Modules provide

Analog Signal Output (Pumps, Detectors)

Remote Control Connector (all)

BCD Connector (Autosampler)

N I N G Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.68 Service Handbook for 1050 Series of HPLC Modules - 11/2001

Common: Cable InformationOverview

Table 14 Cables Overview

Type Description Part NumberService Handbook for 1050 Series of HPLC Modules - 11/2001 69

Analog cables

3390/2/3 integrators 01040-60101

3394/6 integrators, 35900A A/D converter 35900-60750

General purpose (spade lugs) 01046-60105

Remote cables

3390 integrator 01046-60203

3392/3 integrators 01046-60206

3394 integrator 01046-60210

3396A (Series I) integrator 03394-60600

3396 Series II / 3395A integrator, see page 74

3396 Series III / 3395B/96C/97A integrator 03396-61010

1100 / 1050 modules / 1046A FLD / 35900A A/D converter

5061-3378

1040 DAD / 1090 liquid chromatographs / SDM 01046-60202

BCD cables

3392/3 integrators obsolete

3396 integrator 03396-60560

General purpose (spade lugs) 18594-60520

GP-IB cable

1100 module to ChemStation, 1 m 10833A

1100 module to ChemStation, 2 m 10833B

1100 module to ChemStation, 5 m 10833D

Common: Cable InformationAnalog Cables

Analog Cables70 Service Handbook for 1050 Series of HPLC Modules - 11/2001

One end of these cables provides a BNC connector to be connected to 1050 Series modules. The other end depends on the instrument to which connection is being made.

1050 to 3390/2/3 Integrators

1050 to 3394/6 Integrators

Connector01040-60101

Pin3390/2/3

Pin1050 Signal Name

1 Shield Ground

2 Not connected

3 Center Signal +

4 Connected to pin 6

5 Shield Analog -

6 Connected to pin 4

7 Key

8 Not connected


Pin3394/6

Pin1050 Signal Name

1 Not connected

2 Shield Analog -

3 Center Analog +

Common: Cable InformationAnalog Cables

1050 to BNC Connector

Connector8120-1840

PinBNC

Pin1050 Signal NameService Handbook for 1050 Series of HPLC Modules - 11/2001 71

1050 to General Purpose

Shield Shield Analog -

Center Center Analog +


Pin3394/6

Pin1050 Signal Name

1 Not connected

2 Black Analog -

3 Red Analog +

Common: Cable InformationRemote Cables

Remote Cables72 Service Handbook for 1050 Series of HPLC Modules - 11/2001

One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to 1050 Series modules. The other end depends on the instrument to be connected to.

1050 to 3390 Integrators


Pin3390

Pin1050 Signal Name

Active(TTL)

2 1 - White Digital ground

NC 2 - Brown Prepare run Low

7 3 - Gray Start Low

NC 4 - Blue Shut down Low

NC 5 - Pink Not connected

NC 6 - Yellow Power on High

NC 7 - Red Ready High

NC 8 - Green Stop Low

NC 9 - Black Start request Low


1050 to 3392/3 Integrators

N OT


Pin3392/3

Pin1050 Signal Name

Active(TTL)Service Handbook for 1050 Series of HPLC Modules - 11/2001 73


E START and STOP are connected via diodes to pin 3 of the the 3394 connector.







9 7 - Red Ready High

1 8 - Green Stop Low

NC 9 - Black Start request Low4 - Key


Pin3394

Pin1050 Signal Name

Active(TTL)







5,14 7 - Red Ready High


1 9 - Black Start request Low

13, 15 Not connected


1050 to 3396A Integrators


Pin3394

Pin1050 Signal Name

Active(TTL)74 Service Handbook for 1050 Series of HPLC Modules - 11/2001

1050 to 3396 Series II / 3395A Integrators

Use the cable 03394-60600 and cut pin #5 on the integrator side. Otherwise the integrator prints START; not ready.







5,14 7 - Red Ready High





1050 to 3396 Series III / 3395B Integrators


Pin33XX

Pin1050 Signal Name

Active(TTL)Service Handbook for 1050 Series of HPLC Modules - 11/2001 75

1050 to 1050, 1046A or 35900 A/D Converters











Connector5061-3378

Pin1050 /

Pin1050 Signal Name

Active(TTL)

1 - White 1 - White Digital ground

2 - Brown 2 - Brown Prepare run Low

3 - Gray 3 - Gray Start Low

4 - Blue 4 - Blue Shut down Low

5 - Pink 5 - Pink Not connected

6 - Yellow 6 - Yellow Power on High

7 - Red 7 - Red Ready High

8 - Green 8 - Green Stop Low

9 - Black 9 - Black Start request Low


1050 to 1090 LC, 1040 DAD or Signal Distribution Module


Pin1090

Pin1050 Signal Name

Active(TTL)76 Service Handbook for 1050 Series of HPLC Modules - 11/2001





7 4 - Blue Shut down Low

8 5 - Pink Not connected




NC 9 - Black Start request Low5 - Key


PinUniversal

Pin1050 Signal Name

Active(TTL)

1 - White Digital ground

2 - Brown Prepare run Low

3 - Gray Start Low

4 - Blue Shut down Low

5 - Pink Not connected

6 - Yellow Power on High

7 - Red Ready High

8 - Green Stop Low

9 - Black Start request Low

Common: Cable InformationBCD Cables

BCD CablesService Handbook for 1050 Series of HPLC Modules - 11/2001 77

One end of these cables provides a 15-pin BCD connector to be connected to the 1050 Series modules. The other end depends on the instrument to be connected to.

1050 to 3392/3 Integrators (Obsolete)


Pin3392/3

Pin1050 Signal Name BCD Digit

10 1 BCD 5 20

11 2 BCD 7 80

3 3 BCD 6 40

9 4 BCD 4 10

7 5 BCD 0 1

5 6 BCD 3 8

12 7 BCD 2 4

4 8 BCD 1 2

1 9 Digital ground

2 15 + 5 V Low

6 - Key

Common: Cable InformationBCD Cables



Pin3392/3

Pin1050 Signal Name BCD Digit78 Service Handbook for 1050 Series of HPLC Modules - 11/2001


1 1 BCD 5 20

2 2 BCD 7 80

3 3 BCD 6 40

4 4 BCD 4 10

5 5 BCD 0 1

6 6 BCD 3 8

7 7 BCD 2 4

8 8 BCD 1 2

9 9 Digital ground

NC 15 + 5 V Low

Connector18594-60520 Wire Color

Pin1050 Signal Name BCD Digit

Green 1 BCD 5 20

Violet 2 BCD 7 80

Blue 3 BCD 6 40

Yellow 4 BCD 4 10

Black 5 BCD 0 1

Orange 6 BCD 3 8

Red 7 BCD 2 4

Brown 8 BCD 1 2

Gray 9 Digital ground

White 15 +5 Vt Low

In This Book

This manual contains technical information about the Agilent 1050 liquid chromatographs.

This manual is available as electronic version (Adobe Acrobat Reader file) only.


Service Handbook - Pumps (79851A/79852A/B)




Part No. NONE

11/2001

Printed in Germany

Warranty




IMPORTANT NOTE

This version of the 1050 service manual includes all sections from the 01050-90102 edition 4 (1995) and G1306-90102 edition 2 (May 1994). It merges both sections, the MWD and the DAD.

The series I opticals information (79854A MWD) information has been removed (product went out of support during 2000).



44 Pumps: General Information

This chapter provides general information about the 1050 Pumps

Pumps: General InformationIntroduction

This chapter gives general information on

about this pump

repair policy

product structure

capillaries

specifications

About this Manual

This manual provides service information about the 1050 Pumps (isocratic and quaternary). The following sections give the detailed descriptions of all electronic and mechanical assemblies. You will find illustrated part-breakdowns interconnection tables connector configurations as well as all necessary replacement procedures in this manual. Detailed diagnostic procedures using firmware resident test methods and error messages are also given in this manual.84 Service Handbook for 1050 Series of HPLC Modules - 11/2001

Pumps: General InformationAbout the Pumps

About the PumpsService Handbook for 1050 Series of HPLC Modules - 11/2001 85

The 1050 Pump modules houses the mechanical devices and the electronic circuitry for either the isocratic or quaternary module which control the various functions of the flow system. The module is controlled via the user interface through which the operator defines his requirements (flow-composition and so on) and which provides the required analytical information.

Repair Policy

The 1050 Pumps are designed that all components are easy accessible Customers are able to repair certain parts of the 1050 Pumps see Operators Handbook.

For details on repair policy refer to Repair Policy on page 38.

Pumps: General InformationProduct Structure

Product Structure

N OT86 Service Handbook for 1050 Series of HPLC Modules - 11/2001

The 1050 Series of HPLC modules are available in two versions. In the standard version most of the parts used are stainless steel.

In the 1050 Ti Series the flow path of the quaternary pump consists solely of corrosion resistant materials such as titanium, tantalum, quartz, sapphire, ruby, ceramic and fluorocarbon polymers. It is recommended for use with mobile phases containing high salt concentrations, extreme pH solutions and other aggressive mobile phases.

E The isocratic pump was also introduced as Ti - version (79851B) but due to the insufficient orders it became obsolete end of FY 91.

Isocratic Pump 79851A

Quaternary Pump 79852A

Ti - Quaternary Pump 79852B

Pumps: General InformationCapillaries

Capillaries

N OT


In the 1050 Pumps the capillary shipped with the module will have a plastic color coating for identification in terms of material and internal diameter.

All capillaries before the injector have a internal diameter of 0.25 mm. From the injector the internal diameter is reduced to 0.17 mm.

E For the Ti pumps the fittings are always titanium with a titanium nitrite coating and the front and back ferrules are gold plated.

The Ti capillaries have two color coatings. One for identifying the material covering the main part of the capillary and a small one for the internal diameter.

15 Capillary Color Code

color Internal Diameter Material

blue 0.25 mm

green 0.17 mm

red 0.12 mm

white tantalum

Pumps: General InformationSpecifications

Specifications


16 Specifications of 1050 Pumps

Hydraulic System Dual-pistons in-series with proprietary servo-controlled variable stroke drive floating pistons and active inlet valve.

Flow Range Setpoint from 0.001 to 9.999 ml/min in 0.001 ml/min increments.

Piston Displacement 20 to 100 l, automatic matched to flow rate or user-selectable.

Flow Precision


Oven Temperature Range Ambient +5C to ambient +60C in 0.1C increments.Display in C, F or K.

Table 16 Specifications of 1050 PumpsService Handbook for 1050 Series of HPLC Modules - 11/2001 89

Oven Temperature Stability 0.15C

Oven Capacity Two 25-cm or three 20-cm columns.

Control Integrated keyboard with function keys; parameter editing during run possible; keyboard lock; optional control by PC.

Parameters Flow rate, compressibility, stroke volume, upper and lower pressure limits, 2 external contacts; %B, %C, %D (for quaternary pump). Time-programmable Parameters: Flow rate, upper pressure limit, external contacts; %B, %C, %D.

Methods Battery-backed storage of up to 10 methods. Automatic start up and shut down methods. Editing of stored methods possible in run.

Analog Output For pressure monitoring, 2 mV/bar.

Communications Outputs: ready signal and two external outputs (one 24 V relay and one 30V (AC/DC) contact closure, both with 0.25 A. In-and outputs: start, stop and shut down signals. Optional interface for GPIB and RS-232C.

Safety Aids Extensive diagnostics, error detection and display via front-panel LED's and status logbook. User-definable shutdown method activated in case of error. Leak detection and safe leak handling. Low voltages in major maintenance areas. Column pressure protection with maximum rate of pressure change of


Dimensions Height: 208 mm (8.2 in)Width: 325 mm (12.8 in)

Table 16 Specifications of 1050 Pumps90 Service Handbook for 1050 Series of HPLC Modules - 11/2001

For complete description of test conditions used to obtain specifications, see Owners Manual.

Depth: 560 mm (22.0 in)

55 Pumps: Hardware Information

This chapter provides hardware information about the 1050 Pumps

Pumps: Hardware InformationThis chapter gives general and technical information about the hardware components of the 1050 Pumps.

Solvent Cabinet

Pump Hardware

Multi Channel Gradient Valve (MCGV)

Metering Drive Assembly

Pump Head Assembly

Continuous Seal Wash

Active Inlet Valve

Outlet Ball Valve

Frit Adapter Assembly

Purge Valve

High Pressure Damper

Column Holder92 Service Handbook for 1050 Series of HPLC Modules - 11/2001

Pumps: Hardware InformationOverview

OverviewService Handbook for 1050 Series of HPLC Modules - 11/2001 93

The 1050 Pump is based on a dual piston series design which comprises all essential functions a solvent delivery system has to fulfill. Metering of solvent and delivery to the high pressure side are performed by one metering assembly which can generate pressure up to 400 bar.

The basic system (isocratic) comprises the metering assembly including an active inlet valve, an outlet valve, a frit adapter assembly and a damping unit.

The gradient operation system includes a highs peed proportioning valve allowing quaternary operation and a solvent cabinet with separate Helium degassing for each solvent channel.

Since the introduction of the G1303A Online Degasser (December 1,1991) the Helium degassing might be replaced by the degasser module.

A purge valve is installed on the pump head for convenient priming of the pump.

An continuous seal wash is available when the pump is used with buffer solutions. It is mandatory in the Ti - pump and can be ordered as an option for the standard version.

The solvent cabinet for the 1050 Pumps can be equipped with a manual injection valve and a column heater.

Pumps: Hardware InformationOverview

Figure 14 Overview Pump System94 Service Handbook for 1050 Series of HPLC Modules - 11/2001

Pumps: Hardware InformationHow does the Pump Work?

How does the Pump Work?Service Handbook for 1050 Series of HPLC Modules - 11/2001 95

The metering assembly comprises two substantially identical piston pump units. Both pump units comprise a ball screw drive and a pump head with a sapphire piston for reciprocating movement in it. The servo controlled variable reluctance motor drives the two ball drive screws in opposite direction. The gears for the ball screw drives have different circumferences (ratio 2:1) allowing the first piston to move double the stroke length of the second piston. The solvent enters the pump heads close to the bottom limit and leaves it at its top. The outer diameter of the piston is smaller than the inner diameter of the pump head chamber allowing the solvent to fill the gap in between. The first piston has a stroke volume in the range of 20 l to 100 l depending on the flow rate. The microprocessor controls all flow rates in a range of 1 l to 10 ml.

The inlet of the first pumping unit is connected to the active inlet valve which is processor controlled opened or closed allowing solvent to be sucked into the first pump unit. The outlet of the first pump unit is connected via the outlet ball valve and the damping unit to the inlet of the second pump unit. The outlet of the metering assembly is than connected to the following chromatographic system.

Isocratic Operation

When turned on the pump runs through a initialization procedure to determine the upper dead center of the first piston. The first piston moves slowly upwards into the mechanical stop of the pump head and from there it moves back a predetermined path length. The controller stores this piston position in memory. After this initialization the pump starts operation with the set parameters. The active inlet valve is opened and the down moving piston draws solvent into the first pump head. At the same time the second piston is moving upwards delivering into the system. After a controller defined stroke length (depending on the flow rate) the drive motor is stopped and the active inlet valve is closed. The motor direction is reversed and moves the first piston up until it reaches the stored upper limit and at the same time moving the second piston downwards. Then the sequence starts again moving the pistons up and down between the two limits.

Pumps: Hardware InformationOverview of the Electronics

During the up movement of the first piston the solvent in the pump head is pressed through the outlet ball valve and the damping unit into the second pumping unit. The second piston draws in half of the volume displaced by the first piston and the remaining half volume is directly delivered into the system.96 Service Handbook for 1050 Series of HPLC Modules - 11/2001

During the drawing stroke of the first piston the second piston delivers the drawn volume into the system.

Gradient Operation

For gradient operation the multi channel gradient valve (MCGV) connected to solvent containers A, B, C and D is required. The controller makes sure that each intake stroke of the first piston contains the required solvent composition. The controller divides the length of the intake stroke in certain fractions in which the MCGV connects the specified solvent channel to the pump input.

Overview of the Electronics

The figure 2-2 shows the block diagram of the 1050 Pumps including all currently available options.

The common main processor (CMP) controls all functions of the modules. The controller firmware is attached to the relative A/D converter board (RAD).

The column heater can be installed into the solvent cabinet. The electronic control is done via the pump module. Two different boards will be available for supporting the column heater in either the isocratic pump (79851A) or the quaternary pump (79852A/B).

For the quaternary pump (79852A/B) the heater quaternary board (HRQ) controls the column heater and drives the multi channel gradient valve (MCGV).

For the isocratic pump (79851A) the heater isocratic board (HRI) controls only the column heater. The HRI board is a subtract of the HRQ Board; the blank board is identical but the components for the gradient operation are not mounted.

Pumps: Hardware InformationOverview of the Electronics

The communication interface board (CIB) provides an GPIB and RS232C interface. With the CIB installed the pump can be controlled via the ChemStation or via the 3396B integrator.

Figure 15 Block Diagram 1050 PumpsService Handbook for 1050 Series of HPLC Modules - 11/2001 97

Pumps: Hardware InformationOverview of the Flow Path

Overview of the Flow Path

Figur98 Service Handbook for 1050 Series of HPLC Modules - 11/2001

From the bottle head assembly (tube #1) the solvent moves via the gradient valve (MCGV), the connection tube #2 and the active inlet valve into the pump. From the outlet ball valve the capillary #3 is connected to the damper and from there the solvent streams back to the second piston chamber (capillary #4). The standard interface capillary #5 (70 cm long 0.25 mm ID) connects the pump to the next module (for example the autosampler).

In the isocratic pump the solvent sucking tube #2 is directly connected to the solvent bottle and the interface capillary (#5) is connected to the frit adapter.

In the quaternary pump the interface capillary (#5) is connected to a purge valve.

The purge valve allows convenient priming of the system. When opened the flow is directed via tubing (#7) in to the waste.

The typical delay volume for the pump is in the range 900 to 1100 l (depending on system back pressure).

If the seal wash accessory is installed the wash bottle on top of the instrument (tube #6) is connected to the two support rings for back flushing of the piston seals. From the second support ring the wash solvent flows into the collecting vessel.

e 16 Hydraulic Path

Pumps: Hardware InformationSolvent Cabinet

Solvent Cabinet

N OT


Repair Level: Component

The solvent cabinet allows storage of 4 four 1 liter solvent bottles. It is designed to hold the following options:

Helium degassing; later it was replaced by 1050 online degassing

Manual injection valve

Column Heater

Manual injection valve and column heater

Helium Degassing

E For low pressure mixing degassing is a must. Therefore the Helium degassing or the Online Degasser G1303A is mandatory for the quaternary pump.

If the Helium degassing is selected a internal tubing guides the Helium from the back of the solvent cabinet to an on/off valve and from there to four regulators. Each of the regulator supplies helium to one bottle head assembly for separate sparging of each bottle. The bottle head assembly consists of a sintered glass sparger, stainless steel or titanium filter and a cap with vent position. It is designed for the provided standard bottle, but allows also operation with supply bottles from certain vendors. The bottle head assembly has also a connection for a fume hood tubing (see also Helium Degassing Principle on page 269).

17 Product Numbers for Solvent Cabinet

Item Part Number

Solvent Cabinet 79856A

Ti - Solvent Cabinet 79856B


N OT E The connected helium pressure has to be in the range 2 to 4 bar (30 to 60 psi). With pressures below 2 bar the helium degassing system may not work correctly. Pressure above 4 bar might damage the helium regulators.100 Service Handbook for 1050 Series of HPLC Modules - 11/2001

Manual Injection Valve

If ordered with manual injection valve a Rheodyne 7125 valve with 20 l loop will be installed in the solvent cabinet. If ordered as a Ti version a Rheodyne 7125 titanium valve with Tefzel rotor seal will be present in the cabinet. A remote-start output is available at the back of the cabinet.

Column Heater

The column heater fits into the recess of the cabinet. Electronic control is done via the pump module. The column heater can hold up to 25 cm long columns. The flow path of the column heater is stainless steel even in the Ti - version.

The heater uses a heating foil which is attached to a aluminum heating block where the solvent capillaries are leading through (heat exchanger). The column rests in the U-shaped heat exchanger. When turned on, the heat exchanger will heat up the solvent, the column and the surrounding air in the compartment.

Temperature is monitored on the heating block via a Pt. 100. A multi (3) color LED shows the actual status of the column heater. The power consumption of the heater is reduced by heat recycling. Incoming and outgoing capillaries of the heat exchanger are in close thermal contact allowing radial heat exchange while the solvent is streaming through.


Figure 17 Solvent Cabinet including all optionsService Handbook for 1050 Series of HPLC Modules - 11/2001 101

Pumps: Hardware InformationMulti Channel Gradient Valve (MCGV)

Multi Channel Gradient Valve (MCGV)

Figur

Table


Repair Level: Exchange Assembly

The multi channel gradient valve (MCGV) works like a multi position switch. Depending on the timing of the control electronic the Heater Quaternary Board (HRQ) activates one of the four solenoids connecting the selected channel to the output of the valve.

In the Ti-version of the gradient valve only the materials have been changed.

e 18 MCGV

18 Part Numbers MCGV


MCGV 79835-67701 79835-69701

Ti - MCGV 01019-67701

19 Technical Data of MCGV

Switching Time: approximately 2 ms

Solenoid Voltage: +12 V (+36 V Chopper Drive)

Ti Series

Materials in contact with solvent: PFA, PTFE, sapphire, ruby, ceramic, Titanium

Pumps: Hardware InformationMetering Drive Assembly

Metering Drive Assembly

Figur

Table


Repair Level: Exchange Assembly

The metering drive assembly is identical for the stainless steel and the Ti version. The metering pump system is driven by a variable reluctance motor (servo) and electrically controlled by the Pump Drive Control Board (PDC). Feedback about actual movement is sensed by a shaft encoder mounted on top of the motor. In order to achieve required flow resolution a gear is used to transmit motor movement to the two pistons. The gears for the ball screw drives have different circumferences (ratio 2:1) allowing the first piston a twice as large stroke volume as the second piston. The second piston operates with a fixed 180 difference relative to the first piston. A sensor on the motor surface checks for over temperature conditions (90C).

e 19 Metering Drive Assembly

20 Part Numbers Metering Drive Assembly


Metering Drive Assembly 01018-60001 01018-69100

21 Technical Data of Metering Drive

Resolution of mechanical system: 6.6 nl/steps of Encoder

Resolution of Encoder: 0.25 degree

Lowest Frequencies: 2.5 Hz

Highest Frequencies: 25 KHz

Number of steps between piston extension limits: 8191

Pumps: Hardware InformationPump Head Assembly

Pump Head Assembly

Figur

Table



Two identical piston move inside the solvent filled chamber in the pump head assembly. The piston are ball loaded on the spindles and center itself in the seal. The built in spring prevents clearances of the plunger affecting flow accuracy (see also Pump Head Assembly on page 271).

e 20 Pump Head Assembly

22 Part Numbers Pump Head Assembly

Item Part Number

Pump Head Assembly 01018-60004

Ti - Pump Head Assembly 01019-60002

23 Technical Data of Pump Head

Maximum displacement volume: 108 l

Ti - Series

Materials in contact with solvents titanium, gold, sapphire, ceramic

Pumps: Hardware InformationContinuous Seal Wash

Continuous Seal Wash

N OT



Bioscience application do very often use high concentrated buffer solutions. Therefore the seal wash is installed in each Ti pump. For the stainless steel version it is available as an option and should be used when buffer solutions are used in the instrument. If high buffer concentration are used in the pump the continuous s

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