HOXC6 and HOXC8 are potentially novel prognosis predictors of esophageal squamous cell carcinoma Keneng Chen, M.D., PhD, RCSF Luyan Shen, M.D., PhD 2015/4/28 Department of Thoracic Surgery I, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Beijing Cancer Hospital, Peking University School of Oncology, Beijing, China
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HOXC6 and HOXC8 are potentially novel prognosis predictors of esophageal squamous cell carcinoma Keneng Chen, M.D., PhD, RCSF Luyan Shen, M.D., PhD 2015/4/28.
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HOXC6 and HOXC8 are potentially novel prognosis predictors of esophageal squamous cell carcinoma
Keneng Chen, M.D., PhD, RCSFLuyan Shen, M.D., PhD
2015/4/28
Department of Thoracic Surgery I, Key Laboratory of Carcinogenesis and Translational Research (Ministry of
Education), Beijing Cancer Hospital, Peking University School of Oncology, Beijing, China
No potential conflicts of interest were disclosed
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01 The morbidity and mortality remains high
long-term survival remains unsatisfied
Lack of reports with long-term follow-up data from prospective database
Lack of efficient biomarkers for helping to guide the clinical practice
Several issues with the clinical practice of ESCC remain unsolved
prognosis
Why we choose HOX genes ?
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01 There is some similarity between embryogenesis and tumorigenesis
AFP in liver cancer and CEA in colorectal cancer
HOX gene family regulates embryogenesis
HOX genes are deregulated in hematologic malignancy and many solid tumors
Regional expression of transcription factors in the developing gut
Yuasa Y. Nat Rev Cancer. 2003.
Gene Positive cases in ESCC(%)
Positive cases in noncancerous mucosa(%)
HOXA10 3(8.3) 0(0)
HOXA13 27(75) 0(0)
HOXB7 21(58.3) 0(0)
HOXC4 12(33.3) 0(0)
HOXC8 15(41.7) 0(0)
HOXD9 6(16.7) 0(0)
HOXD10 15(41.7) 0(0)
HOXD13 15(41.7) 0(0)
HOXA7 30(83.3) 12(33.3)
HOXA9 24(66.7) 9(25)
HOXC6 24(66.7) 9(25)
HOX genes were abnormally expressed in ESCC (n=36)
Chen KN, et al. Clin Cancer Res. 2005.
potential biomakers
Expression of 11 HOX genes is deregulated in ESCC
CCK8 Assay
Annexin v/PI Cell cycle & Apoptosis
Proliferation
Soft agar colony formation assay
Ability of colony formation
KYSE-450
IHC for HOXC6 、HOXC8 protein detection
Prognosis FFPE tissue specimen
Lentivirus infection
Knockdown of HOXC6/HOXC8
Tumor formation in nude mice
Ability of tumorigenesis
S Group (138 )NC+S Group (136)S+AC Group (148)
Study design and work flow diagram
Staining of IHC
The association of HOXC6/HOXC8 expression with overall survival in naive subgroup (n=138)