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Transcriptionally InactiveChromatin
Transcriptionally ActiveChromatin
SUV39h
MeHP1SUV
39h
MeHP1SUV
39h
MeHP1SUV
39h
MeHP1
MePCPRC1
MePCPRC1
MePCPRC1
MePCPRC1
Me
BPTF
NURF Me
BPTF
NURF
Me Me
Me
Me
Me
Me
Me
Me
Me
Me
MeMeMe
Me
Pericentric HeterochromatinInactive X ChromosomeRb-Mediated Repression
Pericentric Heterochromatin
Inactive X ChromosomeHox Gene Repression Anti-Silencing
Ubiquityl-Histone H2A (Lys119)(D27C4) XP® Rabbit mAb #8240
Normal Rabbit IgG #2729
Ubiquityl-Histone H2A (Lys119) (D27C4) XP® Rabbit mAb #8240: Chromatin IPs were performed with cross-linked chromatin from 4 x 106 NCCIT cells and either 10 µl of #8240 or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003 and primers to the designated loci.
A
0
20
% o
f tot
al in
put c
hrom
atin
GAPDH RPL30 MyoD1
4
2
8
12
6
10
14
MYT-1
Tri-Methyl-Histone H3 (Lys27)(C36B11) Rabbit mAb #9733
Normal Rabbit IgG #2729
16
18
Tri-Methyl Histone H3 (Lys27) (C36B11) Rabbit mAb #9733: Chromatin IPs (A) were performed with cross-linked chromatin from 4 x 106 HeLa cells and either 10 µl of #9733 or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003 and primers to the designated loci. IF analysis (B) of HeLa cells using #9733 (green). Actin filaments have been labeled with DyLight™ 554 Phalloidin #13054 (red).
B
0
45
% o
f tot
al in
put c
hrom
atin
RPL30 GAPDH MyoD1
105
20
30
15
25
35
α Satellite
Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb #9649
Normal Rabbit IgG #2729
40
Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb #9649: Chromatin IPs were performed with cross-linked chromatin from 4 x 106 HeLa cells and either 10 μl of #9649 or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003 and primers to the designated loci.
APPLICATIONS & REACTIVITY KEY: WB Western Blotting / IP Immunoprecipitation / IHC Immunohistochemistry / IF Immunofluorescence / F Flow Cytometry / ChIP Chromatin Immunoprecipitation / -IC Immunocytochemistry / -P Paraffin / -F Frozen / E-P Peptide ELISA / H human / M mouse / R rat / Hm hamster / Mk monkey / C chicken / Mi mink / Dm D. melanogaster / X Xenopus / Z zebra fish / B bovine / Dg dog / Pg pig / Sc S. cerevisiae / All all species expected / ( ) 100% sequence homology