Jan 06, 2018
Histone protein modification
I- Histone acetylation /deacetylationII- Histone phosphrylation/dephosphrylation
Histone protein modification
I- Histone acetylation /deacetylationThe mechanism by which transcriptional activator and transcriptional repressors work. Acetylation results in more opened chromatin structure
Histone protein modification
II- Histone phosphrylation/dephosphrylationPhosphorylated histones are present in transcriptionally active regions.
Mitochondrial DNA
What kind of disease this might be ?
Mitochondrial DNA
This is called as well maternal inheritance
Recombinant DNA technology
How to put gene expression to work An introduction to genetic engineering
Gene cloning
• Insert a new DNA within the genome of a certain organism
• Transformed organism will acquire a new character
What will happen ?if we cut the DNA and inject it into the desired cell
Can we inject the DNA directly into the cell
Identify the gene border first
• ORF Of the gene
Secondly, Identify the promoter area
Types of promoters Endogenous Exogenous
Cut the gene of interst with restriction enzymes
Select a compatible cloning vector
Types of cloning vectors
• Plasmid• Cosmid • BAC• YAC
Expression vectors
• Add a ribosome binding site
Transform into the bacterial cell to replicate the plasmid along with the gene of interest
Competent cells
Example insulin
Cancer genetics
• Types of cancer tumors”• -Benign• Malignant• Metastasis
• DNA of cancer patient is able to transform normal cells into cancerous cells?
Cancer development
• Normal cell needs multiple mutations to develop cancer
• Cancer needs years to occur• One mutated cell can give millions of
mutated cells over years
How cancer develop
Cancer sometimes occurs naturally within the cell without an apparent
reasonDo you think there are other factors
that might induce its development?
•In our bodies, we have up to 50 genes that can be called protooncogenes
•If protooncogene becomes mutated or transformed into oncogene, the host cell becomes malignant
Pyton Rous experiment with chicken ( another meaning of
transformation)
Virus!
Other factors
•Diets •Toxins
•Mutagens •UV and radiation