ull-down assay for Orf6 in Mycobacterium sp. strain JC1 Yeast two hybrid for rv3676-homolog in Mycobacterium sp. strain JC1 imer extension tagenesis idizer onocaridia was deposit in KCCM oraea 16s rDNA sequencing amurella expression in M. tuberculosis ? thern blot or Western blot rmation of transcription level in Mycobacterium sp. strain JC1 (under SNP) CA, orf1-6… rmation of transcription level in Mycobacterium smegmatis (under SNP) expression in Mycobacterium smegmatis
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His pull-down assay for Orf6 in Mycobacterium sp. strain JC1 -> Yeast two hybrid
His pull-down assay for Orf6 in Mycobacterium sp. strain JC1 -> Yeast two hybrid Study for rv3676 -homolog in Mycobacterium sp. strain JC1 1. Primer extension 2. Mutagenesis CO oxidizer Pseudonocaridia was deposit in KCCM Pandoraea 16s rDNA sequencing Tsukamurella - PowerPoint PPT Presentation
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His pull-down assay for Orf6 in Mycobacterium sp. strain JC1 -> Yeast two hybrid
Study for rv3676-homolog in Mycobacterium sp. strain JC11. Primer extension2. Mutagenesis CO oxidizer Pseudonocaridia was deposit in KCCM Pandoraea 16s rDNA sequencing Tsukamurella
CO-DH expression in M. tuberculosis ? Northern blot or Western blot
Confirmation of transcription level in Mycobacterium sp. strain JC1 (under SNP) cutBCA, orf1-6…
Confirmation of transcription level in Mycobacterium smegmatis (under SNP)
CO-DH expression in Mycobacterium smegmatis
CutR expression pMal C2X vector 이용 -> sequencing 의뢰
Searching the anti-tuberculosis drug from natural compound 흰쌀 , 지골피 , 측백잎 , 황경피나무껍질 , 마늘 , 고삼 , 백급 , 대추 , 의이인
분자설계연구소 200 개의 compound test. test 완료 . XDH inhibitor Allopurinol, oxypurinol, BOF 4272, FYX, Y-700, TEI-6720
CO-DH purification from Mycobacterium smegmatis
Mutagenesis for das gene Searching the single crossover mutant candidates
CO-DH expression in Mycobacterium sp. strain JC1 mod mutant
Mycobacterium smegmatis mc2-155
No cbbLS gene in database has key enzymes for reverse TCA cycle
PCR product for cbbLS gene in Mycobacterium smegmatis mc2-155 wild type, orf2- mutant, and orf4- mutant.