WAC™ NMR SPR TS Throughput ++ + ++ ++ Info Content ++ ++ + - Quality Control ++ ++ - - Cost efficiency ++ - - ++ Principles of WAC™ • Target protein immobilized on HPLC column • Physiological buffer as mobile phase • Detection by LC-MS • Retention time of fragments related to selective interactions with target • Kd calculated from retention time • Allows simultaneous identification of weak binders (mM) in complex mixtures Advantages of WAC™ WAC has significant advantages compared to the other methods in terms of speed and information provided. WAC™ fragment screening is now an integral part of our FBLD services which also includes: • NMR screening/validation • X-ray co-crystallization • Medchem fragment expansion/elaboration • In vitro biology/ADME We operate under an exclusive license of the proprietary WAC™ technology and offer this as a unique service Contact info Red Glead Discovery AB | SARomics Biostructures AB Medicon Village, SE-223 81 Lund, SWEDEN www.redglead.com | www.saromics.com RED GLEAD DISCOVERY High-throughput fragment screening by WAC™ Schematic representation of WAC™