HITECH DIAGNOSTIC CENTRE USER INFORMATION Document No : HTDC/File/Test Information/4.7/F08 – C8 and user locations Issue No :2 Page 1 of 85 Issue Date :03.01.2017 Prepared by: QM Approved by: Lab Director Location of the laboratory; Location of HI.TECH DIAGNOSTIC CENTRE HI TECH DIAGNOSTIC Centre is situated in the heart Kochi.The centre is located at Palarivattom Janatha bus stop, Behind canara bank on Edapally- Palarivattom road.Any one can easily access the centre.If you coming from Aluva get down at janatha bus stop.From ernakulam just get down at kaloor stadium stop ,walk forward.can see the lab on the right side of the road Opening hours - morning 5.00 am to 11pm. Types of clinical services offered by the laboratory including examinations referred to other laboratories; BIO CHEMISTRY SI NO PARAMETER METHOD USED ALBUMIN DRY CHEMISTRY ALKALINE PHOSPHATASE DRY CHEMISTRY ANA IFA ELISA Anti DS DNA ELISA ANTI CCP CMIA ANTI HCV ELFA ANTI THYROGLOBULIN ANTIBODY CMIA ANTI HEV IgG ELISA AFP ELIFA BETA HCG ECI CALCIUM DRY CHEMISTRY CANCER ANTIGEN-125(CA-125) ELFA CEA CMIA HI-TECH DIAGNOSTIC CENTRE Behind Canara Bank, Plarivattom Janatha, Kochi, Kerala, India, Pin- 682019 0484 2343113 / 2341441 [email protected]
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HITECH DIAGNOSTIC CENTRE USER INFORMATION
Document No : HTDC/File/Test Information/4.7/F08 – C8 and user locations Issue No :2
Page 1 of 85 Issue Date :03.01.2017
Prepared by: QM
Approved by: Lab Director
Location of the laboratory; Location of HI.TECH DIAGNOSTIC CENTRE HI TECH DIAGNOSTIC Centre is situated in the heart Kochi.The centre is located at Palarivattom Janatha bus stop, Behind canara bank on Edapally- Palarivattom road.Any one can easily access the centre.If you coming from Aluva get down at janatha bus stop.From ernakulam just get down at kaloor stadium stop ,walk forward.can see the lab on the right side of the road
Opening hours - morning 5.00 am to 11pm.
Types of clinical services offered by the laboratory including examinations referred to other laboratories; BIO CHEMISTRY
SI NO PARAMETER METHOD USED
ALBUMIN DRY CHEMISTRY
ALKALINE PHOSPHATASE DRY CHEMISTRY
ANA IFA ELISA
Anti DS DNA ELISA
ANTI CCP CMIA
ANTI HCV ELFA
ANTI THYROGLOBULIN ANTIBODY CMIA
ANTI HEV IgG ELISA
AFP ELIFA
BETA HCG ECI
CALCIUM DRY CHEMISTRY
CANCER ANTIGEN-125(CA-125) ELFA
CEA CMIA
HI-TECH DIAGNOSTIC CENTRE
Behind Canara Bank, Plarivattom Janatha, Kochi, Kerala, India, Pin- 682019 0484 2343113 / 2341441 [email protected]
HITECH DIAGNOSTIC CENTRE USER INFORMATION
Document No : HTDC/File/Test Information/4.7/F08 – C8 and user locations Issue No :2
Page 2 of 85 Issue Date :03.01.2017
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CORTISOL CMIA
CA19.9 ELFA
CHOLESTEROL DRY CHEMISTRY
CREATININE DRY CHEMISTRY
C.PEPTIDE CMIA
DHEAS CMIA
ESTRADIOLE (E2) ELFA
FASTING BLOOD SUGAR DRY CHEMISTRY
FERRITIN ECI
FREE TRIODTHYRONINE (FT3) CMIA
FREE THYROXINE (FT4) CMIA
FSH CMIA
GCT DRY CHEMISTRY
GAMMA GT G.GLUTAMYL-P-NITROANILIDE
HDL CHOLESTEROL DRY CHEMISTRY
HBA1C TOSOH HLC GX723
HBSAG CMIA
HIV1&2 ECI
HSV IgG ELISA
HSV IgM ELISA
IMMUNOGLOBULIN(IgE) ELFA
IRON DRY CHEMISTRY
INTACT PARATHYROID CMIA
INSULIN (POSTPARANDIAL) CMIA
INSULIN CMIA
LH ECI
LIPID PROFILE DRY CHEMISTRY
LITHIUM DRY CHEMISTRY
LFT DRY CHEMISTRY
MICROALBUMINUREA STRIP METHOD
PROGESTERONE ELFA
PROLACTIN ECI
PSA ECI
PHOSPHORUS DRY CHEMISTRY
POTTASSIUM DRY CHEMISTRY
RA FACTOR TURBIDOMETRIC
RANDOM BLOOD SUGAR DRY CHEMISTRY
RUBELLA IgG, IgM ELFA
SODIUM DRY CHEMISTRY
SGOT DRY CHEMISTRY
SGPT DRY CHEMISTRY
TRIGLYCERIDE DRY CHEMISTRY
TIBC DRY CHEMISTRY
TORCH IgG,IgM ELFA
TOXOPLASMA IgG,IgM ELFA
TOTAL BILIRUBIN DRY CHEMISTRY
TOTAL PROTIEN DRY CHEMISTRY
T3 CMIA
T4 CMIA
TSH CMIA
TESTO STERONE ELFA
UREA DRY CHEMISTRY
URIC ACID DRY CHEMISTRY
VIT D 25OH ECI
VIT-B12 CMIA
HAEMATOLOGY
SI NO TEST NAME METHOD
HITECH DIAGNOSTIC CENTRE USER INFORMATION
Document No : HTDC/File/Test Information/4.7/F08 – C8 and user locations Issue No :2
Instruction for filling request form Clinician request form Ref no-----.The clinician has to fill the column with the unique op/ip number of the patient. Patient name………patient name is filled either in English or in Malayalam. Age ……..The patient age is filled in years or month. Gender…..male/female or boy /girl. Adress…….in this patients current address is filled . Phone number….either patients landline number or mobile phone number is added.
HITECH DIAGNOSTIC CENTRE USER INFORMATION
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Approved by: Lab Director
Ref by dr…….prescribed doctors details( name and hospital or clinic). Clinical details……The previous clinical history of the patient is filled. Test details…..The clinician has to tick which test has to be performed
Patient request form Patient name………patient name has to fill name either in English or in Malayalam. Age …….. age is filled in years or month. Gender…..has to tick /write ,either male/female or boy /girl. Date of birth.the patient has to write date,month and year of birth in this column. Adress…….patient is aked to fill the current address Phone number….either patients landline number or mobile phone number is added. Ref by Doctor…….prescribed doctors details( name and hospital or clinic). Test details…..The patient has to tick which test has to be performed.
PATIENT PREPARATION 1. PATIENT PREPARATION FOR BLOOD SAMPLES:- Specified fasting period is strictly followed for the following parameters:- a) Fasting glucose Preparation: This test requires an overnight fast. One should wait to eat and/or take a hypoglycemic agent (insulin or oral medication) until after sample has been drawn, unless told otherwise. Overnight fasting –minimum 9 hrs is required (Maximum 9-12 hrs) Delayed Fasting-As asked by the referring Clinician/Doctor b) Lipid profile Fasting period preferably 12 hrs; minimum (14 hrs) b) Post prandial glucose Blood specimen is collected 2hrs after meal is taken. d) Glucose Tolerance test
a) The patient should be on a normal diet containing normal daily requirement of carbohydrate (i.e. approximately 150gm per day) at least for 3 days prior to the test.
b) patient must come to the laboratory after overnight fasting c) The individual should not eat food ,drink tea or coffee, vigorously exercise or smoke cigarettes
during the test d) Since five blood specimen have to be collected at an interval of 30 minutes each the patient has
be made aware of the waiting period of 2-3 hrs at the collection center . e) 75 gm of glucose to be taken orally immediately after giving fasting blood and urine glucose
The next blood and urine sample to be collected at 30 minutes,1 hr 1 1/2 and 2 hrs after ingestion of glucose /as asked by the Referring Clinician/Doctor.
f) for pregnant patient 100 gm glucose is given instead of 75 gm. A complete 3 hrs oral GTT is done by taking blood and urine samples at 1 hour interval.
G) Timed collection
HITECH DIAGNOSTIC CENTRE USER INFORMATION
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For some hormonal studies, specimen is collected at specific timings .For S. cortisol: Blood specimen is collected at 8 A M &4.00 .The timing of Blood collection has clinical significance and hence must be clearly specified on the container. General instruction for patients scheduled for phlebotomy Following things may after test result, which should be kept in mind.
. Physical activities may influence the following parameters temporarily such as alkaline phosphates, creatinine kinase, Alkaline Amino Transfer raise (ALT) ,Lactate Dehydrogenate (LDH) that may usually return to normal after few hours.
.In general patients scheduled for phlebotomy should refrain from strenuous physical activity. alcohol, drugs or changes in diet for 24 hours prior to be ready for the procedure.
2.PATIENT PREPARATION FOR URINE SAMPLE :- a)Random Urine Specimen :- .First morning specimen whenever possible .Urine for pregnancy Test should preferably be first morning void sample .Specimen should be clean catch mid-stream sample .Specimen should be clean catch mid stream sample.
Specimen should be collected in the sterile container provided by Diagnostic Center Pathology department)
. c)24 hours urine collection
An unbreakable ,5 liter (approx) plastic container with proper preservative is used
Unless the physician indicate otherwise ,the patient is instructed to maintain usual amount of liquid intakes
Patient is asked not to consume alcohol
Instruct the patient that the preservative in the 24 hours container should not be discarded
Container should be kept in a refrigerator or at a cool place during the 24 hour collection period
Collect each void in a smaller container and carefully pour the urine into the 24 hours container .Do not pass urine directly in to the 24 hours container
On the day of collection, the patient must empty his/her bladder of first morning urine into toilet. (not to be included in the 24 hours collection ) Write the date and time of voiding, on the container label.
Add all subsequent voided into the container. The last sample collected should be the first specimen voided the following morning at the same time as the previous morning first voiding.
Submit the 24 urine container to the Diagnostics Center Laboratory Within one hour.
HITECH DIAGNOSTIC CENTRE USER INFORMATION
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Approved by: Lab Director
d) Urine collected instruction about pediatric patient
Parents are instructed verbally to avoid contamination with Stool STOOL FOR OCCULT BLOOD Patient is instructed to avoid the following food item and drugs for 3 (three) days before and during the stool collection period:-
Red meat
Citrus fruits containing vitamin C , e.g. :Lemon, Orange etc
Anti –inflammatory drugs/NSAID
Iron supplements (iron tonics)
Specimens collected prior to or during menstrual period, bleeding hemorrhoids or haematuria are un acceptable
HITECH DIAGNOSTIC CENTRE USER INFORMATION
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COLLECTION OF STOOL
Give the patient a disposable wide mouthed plastic container to pass the specimen.
Patient should be asked to avoid contaminating the stool with urine . SEMEN
Abstinence for 3 to 5 days Just before you collect
Urinate
Wash genital area using WATER ONLY. Do not use soap on the genital area prior to collection .Soap may kill the sperm
Remove cap from the specimen container immediately prior to collection. Avoid touching the inside of the container or the inside of the container lid
Obtain the semen sample by masturbation only. Do not use saliva as a lubricant. The only acceptable lubricant is liquid glycerin. To avoid contamination, don’t open the container until just prior to ejaculation.
Ejaculate directly into the specimen container. ( The penis should not touch the inside of the container ).
Collect the entire ejaculate
Replace the container lid as soon as specimen is collected to avoid contamination Submit the Specimen to laboratory with in 30 minutes.
Sputum Specimen: Early morning specimen in a sterile disposable wide mouthed plastic container is preferred Which should be collected in a container provide by Diagnostic Center Laboratory Random sample can also be acceptable Instruction to the patient:
The mouth to be rinsed well by using water
The sputum must be coughed up from the lungs or bronchi the container to be recapped immediately and tightly.
The patient should be explained clearly the difference between saliva and sputum The patient needs washing his mouth three times with drinking water. He should
spit out all residual saliva .He/she is asked to cough forcefully at 10 times for collecting sputum. At least 10 cc. of sputum is necessary for smear and culture.
Sputum mixed with saliva is unsatisfactory for reporting and needs a repeat sample In non-ambulatory patient, through cleaning of mouth with sterile wet cotton is
mandatory before sputum collection Collection of effusions or fluids like pleural, pericardial, ascities, hydrocele fluids as a rule done
by the clinical consultants.
HITECH DIAGNOSTIC CENTRE USER INFORMATION
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Approved by: Lab Director
PRIMARY SAMPLE STORAGE REQUIREMENTS TEST WISE
Sl.
no
TEST SPECIMEN/AMOUN
T
ADDIT
IVES
CONTAINE
R
STORA0GE/STAB
ILITY
1 Alanine Aminotransferase
ALT/SGPT
2 ml clotted blood - Plain Vial With red
top
Stable in whole blood at room
temperature for 12-
24 hrs. Stable in serum at 2-8 c up
to 3 weeks
2 Albumin 2 ml clotted blood - Plain Vial With red
top
Stable in whole blood at room
temperature or at 8
hrs. Stable in serum at 2-8 c up
to 2 weeks
3 Alkaline Phospharase
2 ml clotted blood - Plain Vial With red
top
Stable in whole blood at room up
to 12 hrs. Stable in
serum at 4 c up to 1 weeks
4 Aspartate
Aminotransferase AST/SGOT
2 ml clotted blood - Plain Vial
With red top
Stable in whole
blood at room temperature for 12
hrs. Stable in serum at 4c c up to
3 3weeks
5 Widal 2 ml clotted blood - Plain Vial
With red top
Stable up to 1
week stored at 8c
6 Bilirubin, total Bilirubin, Direct
2 ml clotted blood plain Vial with red top
Protect from light .stable in Whole
blood at room temp for 3 hrs Stable in
serum at 2-8 c up to 1 week
7 VDRL 2 ml clotted blood plain Vial
with red top
Stable is stored at
2-8 degree
8 Cholesterol/LDL
Cholesterol/
2 ml clotted blood plain Vial
with red top
Stable in Whole
blood at room
HITECH DIAGNOSTIC CENTRE USER INFORMATION
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HDL Cholesterol tempt or at 2-8 c up to 12 hrs
stable in serum at
2-8 c up to 72 hrs
9 Creatinine 2 ml clotted blood plain Vial with red top
Stable in Whole blood at room temp
or at 2-8 c up to 12hrs stable in
serum at 2-8 c up
to 24 hrs
10 Aso titre 2 ml clotted blood plain Vial with red top
Stable When stored at 2-8 degree for 1
1week
11 Glucose 1ml anticoagulated
blood(fasting,post prandial,
T6randomspecime Do not collect blood
from an arm recevingan i.v.
infution.
Vial with
greens Top
Stable in fluoride /
oxalate blood at room temp up to 3
hrs. Stable in plasma at2-8c for
up to 48 hrs
12 Ra factor 2 ml clotted blood plain Vial
with red top
Stable When stored
at 2-8 degree for 1 1week
13 Protein, total 2 ml clotted blood plain Vial
with red top
Stable in Whole
blood at room tempt up to 4hrs
stable in serum at
room temp for 4hrs at 2-8 c for up to 3
3days
14 CRP 2 ml clotted blood plain Vial with red top
Stable When stored at 2-8 degree for 1
week
15 Semen Patient should collect
full amount of an ejaculation
Clean dry
disposable container
Test as soon as
possible
16 Blood group & Rh 2ml anti coagulated
blood
EDTA with
violet top
Stable When stored
at 2-8 degree for 1 1week
HITECH DIAGNOSTIC CENTRE USER INFORMATION
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17 Triglycerides 2 ml clotted blood (fasting specimen) 12-
14 hrs
plain Vial with red top
Stable in Whole blood at room
tempt or at 2-8 c to
3hrs .Stable in serum at 2-8 c up
to 72 hrs
18 Uric acid 2ml clotted blood plain Vial with red top
Protect from daylight .stable in Whole blood at
room temp up to 12 12hrs
19 Urea 2ml clotted blood Plain Vial
With Red top
Stable in whole
blood at room temperature or at
2-8 c up to 12 hrs.
Stable in serum at 2-8 c up to 48 hrs
20 Bence jones
proein
24 hrs urine
preservative Boric acid 10-15 gm
5 liter
plastic container
Test as soon as
possible Refrigerated at if
the test is not done
within 1 1hrs
21 Bile pigment 5-10 ml urine Clean dry plastic
container
Test as soon as possible
Refrigerated at if the test is not done
within 1 1hrs
22 Glucose 5-10 ml urine Clean dry
plastic container
Test as soon as
possible Refrigerated at if
the test is not done within 1 1hrs
23 Occult Blood 5-10 ml urine Clean dry plastic
container
Test as soon as possible
Refrigerated at if the test is not done
within 1 1hrs
24 Ketones 5-10 ml urine Clean dry plastic
Clean dry
container
Test as soon as possible
Refrigerated at if
the test is not done
HITECH DIAGNOSTIC CENTRE USER INFORMATION
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within 1 1hrs
25 pH 5-10 ml midstream urine
Clean dry container
Test as soon as possible
Refrigerated at if
the test is not done within 1 1hrs
26 Protein
(qualitative)
5-10 ml urine Clean dry
plastic container
Test as soon as
possible Refrigerated at if
the test is not done
within 1 1hrs
27 Sputum AFB No significance in Quantity Patient
should take sputum in a deep cough
Sterile disposable
container
Test as soon as possible
28 Occult blood Collect a small sample patient should not eat
red meat or vegetables containing
citrus food for 3 days before the test
Clean dry plastic
container
Test as soon as possible
Refrigerated at if the test is not done
within 1 1hrs
29 R/E 10 ml Clean dry plastic
container
Test as soon as possible
Refrigerated at if the test is not done
within 1 1hrs
30 TC,DC,HB, PLATELET COUNT
PCV,MALARIAPARASITE ,CBC
EDTA bottle 2 ml blood
Violet top container
Test on the same day
31 ESR 3.8 sodium citrate bottle 1.6 ml Blood
Tube with Black Top
contain 0.4
ml sodium citrate(3.8
%)
Test as soon as possible
HITECH DIAGNOSTIC CENTRE USER INFORMATION
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32 Bile Salt 10 ml urine Sterile disposable container
Test as soon as possible
33 Urine microscopy 10 ml urine Sterile disposable container
Test as soon as possible
34 Bence Jones Protein
30 ml urine Sterile disposable container
Test as soon as possible
35 MP stix 2 ml EDTA blood EDTA bottle with violet
top
Test as soon as possible
36 HIV 2 ml clotted blood Red Top clot
activator
Stable at 2-8 c for 1 week
37 HbsAg 2 ml clotted blood Clot activator
with red cap
Stable at 2-8 c for 1 week
38 HCV 2 ml clotted blood Clot activator
with red cap
Stable at 2-8 c for 1 week
HITECH DIAGNOSTIC CENTRE USER INFORMATION
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39 HbAIC 2 ml EDTA blood EDTA with violet top
Test on the same day
40 Urine micro albumin
30 ml urine Sterile Plastic
container
Test on the same day
Out patient sample collection Patient contact the receptionist on duty
The receptionist reads the prescription/requisition from ,and inform the patient if any of
the required tests not done at laboratory If patient accepts it he/she is informed about the charges and probable time of getting
the report Cash Memo is generated.
The receptionist informs the patient that the bill must be brought for collecting the
report The patient is escorted to the sample collection room
All collected samples are submitted to the respective divisions for testing
Special Timing of Collection:
SL NO TEST PATIENT CONDITION
1 Any time any test of biochemistry except sugar and lipid profile
Any time (P.F)
2 Glucose Fasting for FBS
3 P P PP two hours after meal
or heavy breakfast
4 Random Anytime
5 Lipid profile 12 hours fasting
(minimum 9 hrs)
6 HB, TC, DC, ESR, PLATELET, Malaria CBC Any time
7 Any serological test Any time(preferably fasting)
8 Microscopy and bio-chemistry Any time
HITECH DIAGNOSTIC CENTRE USER INFORMATION
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9 Urine Routine Any time(preferably first morning sample)
10 Stool for Routine Any time
11 Stool for OBT Any sample. The patient should avoid red meat
,citrus foods, iron tonic & anti –inflammatory drugs for for3 days
12 Hormone tests Preferably fasting sample
Special handling needs between time of collection and time received by the Laboratory:
Transport of blood, urine and others fluids and tissue specimen from collection site to the laboratory is important and few precautions are necessary during this period.
1. Specimen should be received by the laboratory staff within 30 minutes of
collection to allow timely completion of specimen processing 2. Avoid agitation of blood specimen to minimize hemolysis
3. Specimen should be protected from direct exposure to light ,Which cause breakdown of certain analyses e.g. Bilirubin
4. For analyses of unstable constituents, such as ammonia and acid phosphates, Specimens must be sent to the laboratory immediately after collection. Out side sample should be kept immediately at 4 c &transported on ice
5. All Laboratory specimens must be transported in a safe and convenient manner to prevent biohazard exposure or contamination of the specimen.
6. Specimen requiring refrigeration must be maintained between 2 c and 8 c and can be carried in an insulated container
7. Large volume urine specimen should be collected in a leak proof,5L container
8. The samples are transported in a box with test tube holding facility and the facility for holding urine/motion bottles.
Any requirements for patient consent (e.g. Consent to disclose clinical information and
family history to relevant healthcare professionals, where referral is needed). If any test like genetic test done by the referral laboratory require consent and/or family history, patient has
to comply with the requirements. The patients and users will be provided with adequate information on the clinical procedures
to be performed with an informed consent
HITECH DIAGNOSTIC CENTRE USER INFORMATION
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Sample rejection and acceptance criteria Sample are rejcted ,if Mismatched name or unique patient/sample identifier on test requisition and Specimen. No name or unique patient/sample identifier on test requisition. Incorrect container, device or transport media for test ordered . Haemolysed sample Highly lipemic sample. Delayed Sample. Unadequate quanitity of sample. Criteria for sample collected by patient Mismatched name or unique patient/sample identifier on test requisition and Specimen. No test requisition provided (if traceable) If the written documentation is not received, the specimen will be rejected No specimen/sample received Damaged – improper transport media Specimen/sample type unacceptable for test Quantity is not sufficient . Damaged due improper temperature condition Hemolyzed No test ordered on requisition Lipemic Damaged – too old No specimen/sample collection date on requisition Damaged ‐ broken or leaked sample No specimen/sample ID No specimen/sample collection site on requisition Damaged ‐ contaminated Damaged – expired transport media
Availability of clinical advice on ordering of examinations and on interpretation of examination results If any clarifications or clinical advice is needed, patient or clinician may call the laboratory. Simple queries will be handled by the receptionist. If further technical clarification is needed, the concerned section/personnel will address the query.
HITECH DIAGNOSTIC CENTRE USER INFORMATION
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The laboratory’s policy on protection of personal information
HI-TECH DIAGNOSTIC CENTRE has no involvement in any activities that would diminish confidence in the laboratory’s competence, impartiality, judgment or operational integrity. HI-TECH DIAGNOSTIC CENTRE is free from any undue commercial, financial, or other pressures and influences. We do not have formal tie up with any companies. We ensure that potential conflicts in competing interests if any are openly and appropriately declared and we don’t have any potential conflicts in competing interests. We follow appropriate procedures to ensure that our staffs treat human samples, tissues or remains according to relevant legal requirements. We ensure that confidentiality of information is maintained. A declaration to this effect has been signed by all the personnel. The laboratory’s complaint procedure
HITECH DIAGNOSTIC CENTRE have a documented procedure for the management of complaints or other feedback received from clinicians, patients, laboratory staff or other parties.
The complaint/suggestion box is kept in the reception. The complaint register is available in the reception. The receptionist check the complaint box every day for complaints. If any complaint, he/she reports it to the laboratory director. The complaints can be made orally or over phone also. The person attending the user directly or over the phone immediately registers that complaints in complaint book with available details and report it to laboratory director. There is provision for making complaint in the web site also. The web site complaint will be monitored by managing director. The negative feedback is also taken as complaint. The feedback is analyzed by the quality manager and the negative comments are taken as complaints.
In case of complaint, the laboratory director, delegate the appropriate person to record and analyze the complaint. The laboratory directors, with other personnel, propose and implement immediate remedy. Then, a root cause analysis is done and appropriate long term solutions are formulated and implemented. The laboratory director will report the necessary details to the person who made the complained. Records of all complaints and their investigation and the action taken are maintained. Test information
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ALBUMIN
Test information Albumin is the main protein in the plasma. It is decreased in cirrhosis, malnutrition and nephorotic syndrome.
primary sample volumes 1mL special precautions The sample should be taken in comfortable sitting for 3
Test information It is an enzyme present in serum. It is increased many fold in bone forming conditions like growing children, healing fracture, rickets, osteomalacia and Pagets disease. It is also increased in obstructive jaundice
Test information Anti nuclear antibody – the test done to diagnose immune disorders like Systemic Lupus Erythromatosis, Rheumatoid arthritis, Ankylosing spondylitis, etc
Test information Anti double strand DNA antibody – the test done to diagnose immune disorders like Systemic Lupus Erythromatosis, Rheumatoid arthritis, Ankylosing spondylitis, etc
Test information estrogen primary sample volumes 2 mL special precautions nil
turnaround time 1hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference Other steroids
FASTING BLOOD SUGAR
Test information This is used to diagnose diabetes mellitus. Normal persons will have a level less than 100mg/dL. If it is more than 126mg/dL Diabetes mellitus may be diagnosed
primary sample volumes 1 mL
special precautions nil
turnaround time 1hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference Total protein
FERRITIN
Test information Iron load primary sample volumes 2 mL
special precautions nil
turnaround time 1hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference Hemosiderin, heme
FREE TRIODTHYRONINE (FT3)
Test information Free T3 primary sample volumes 2 mL
special precautions nil turnaround time 1hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference Reverse T3
FREE THYROXINE (FT4)
Test information Free T4 primary sample volumes 2 mL
special precautions nil
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turnaround time 1hr biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference RT3
FSH
Test information Follicle stimulating hormone primary sample volumes 2 mL
special precautions nil
turnaround time 1hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference LH, TSH
GCT
Test information Screening for gestational diabetes mellitus primary sample volumes 2 mL
special precautions nil
turnaround time 1hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1 interference protein
GAMMA GT
Test information For alcoholic hepatitis and obstructive jaundice primary sample volumes 2 mL
special precautions nil turnaround time 1hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference glutamate
HDL CHOLESTEROL
Test information Reverse cholesterol transport primary sample volumes 2 mL
special precautions nil
turnaround time 1hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference Apo A
HBA1C
Test information This gives information about long term control of blood glucose level. For diabetic the level is more than 6.5%. Normal people will have level less than 5.6%. If it is in between, it is prediabetes,
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primary sample volumes 2 mL special precautions nil
turnaround time 2hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference HbS
HBSAG
Test information Viral infection of liver primary sample volumes 2 mL
special precautions nil
turnaround time 1hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1 interference Other surface antigens
HIV1&2
Test information Screening for AIDS primary sample volumes 2 mL
special precautions nil
turnaround time 1hr biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference Severe immunodepression
HSV IgG
Test information Viral infection marker primary sample volumes 2 mL
special precautions nil
turnaround time 1hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference Surface antigens
HSV IgM
Test information Current viral infection primary sample volumes 2 mL
Test information Inulin surge primary sample volumes 2 mL
special precautions nil
turnaround time 1hr biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference Administered insulin
INSULIN
Test information Fasting insulin level primary sample volumes 2 mL
special precautions nil
turnaround time 1hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference Insulin and c peptieds
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LH
Test information Ovulation marker primary sample volumes 2 mL
special precautions nil
turnaround time 1hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference FSH, TSH
LIPID PROFILE
Test information This is a group of test – total cholesterol, HDL cholesterol, LDL cholesterol, Triglyceride, VLDL cholesterol and LDL/HDL ratio. This is useful in managing the patient with diabetes mellitus, ischemic heart disease etc
primary sample volumes 2 mL
special precautions Fasting sample should be given
turnaround time 1hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference Refer individual tests
LITHIUM
Test information This is drug monitoring. The patient with maniac depressive illness is treated with Lithium. The therapeutic window is narrow.
primary sample volumes 2 mL
special precautions nil
turnaround time 1hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference Na,H+
LFT
Test information This is a group of test to assess the liver function. Albumin is produced in liver and its level shows the synthetic function of the liver. AST and ALT are transaminase enzymes. If elevated, the liver cell destruction may be inferred. The bilirubin level gives information about severity and type of jaundice. Alkaline phosphatase and gamma glutamyl transferase are elevated in obstructive jaudice
primary sample volumes 3 mL
special precautions nil turnaround time 1hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference Refer individual test
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MICROALBUMINUREA
Test information If the urine albumin level is between 150mg/day to 300mg/day, it is called as micro albuminuria. This shows that the glomerular damage has begun in diabetic patient which can be slowed by ACE inhibitors.
primary sample volumes 2 mL
special precautions nil
turnaround time 1hr biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference Ca
PROGESTERONE
Test information Hormone level to support pregnancy primary sample volumes 2 mL
special precautions nil
turnaround time 1hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference Other steroids
PROLACTIN
Test information Fertility screening primary sample volumes 2 mL
Test information This is an important electrolyte in serum. Both elevated and decrease level are fatal. The levels are altered in diarrhea, vomiting, dehydration, renal disorders, diuretic therapy etc
primary sample volumes 2 mL
special precautions nil
turnaround time 1hr biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference Sodium, hydrogen ion
RA FACTOR
Test information For rheumatoid arthritis primary sample volumes 2 mL
special precautions nil
turnaround time 1hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference Other antibodies
RANDOM BLOOD SUGAR
Test information More than 200mg/dL – diabetes mellitus Less than 50mg/dL - hypoglycemia
primary sample volumes 1 mL
special precautions nil
turnaround time 1hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference Total protein
RUBELLA IgG,IgM
Test information Screening women primary sample volumes 2 mL
special precautions nil
turnaround time 1hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference Other antigens
SODIUM
Test information Important electrolyte in serum.Both increased and decreased condition affect brain.
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primary sample volumes 2 mL special precautions nil
turnaround time 1hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference Hydrogen ion
SGOT
Test information This is a transaminase enzyme. This is elevated in liver cell destruction. This may be elevated in other conditions like hemolysis also.
primary sample volumes 2 mL
special precautions nil
turnaround time 1hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference nil
SGPT
Test information This is a transaminase enzyme. This is elevated in liver cell destruction. This may be elevated in other conditions like hemolysis also.
primary sample volumes 2 mL
special precautions nil
turnaround time 1hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference nil
TRIGLYCERIDE
Test information This is an estimate of fatty acid in serum. In fasting state it is an estimate of VLDL. In fed state chilomicron also contribute foe the triglyceride level. Triglyceride level in fasting sample is elevated in diabetes mellitus.
primary sample volumes 2 mL
special precautions nil
turnaround time 1hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference Free glycerol
TIBC
Test information This is an estimate of transferrin level in the serum. This used to analyze the severity of iron deficiency or excess.
primary sample volumes 2 mL
special precautions nil
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turnaround time 1hr biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference Desferol, imferon
TORCH IgG,IgM
Test information Screening women primary sample volumes 2 mL
special precautions nil
turnaround time 1hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference Other antigens
TOXOPLASMA IgG,IgM
Test information Diagnosis of Toxoplasmosis primary sample volumes 2 mL
special precautions nil
turnaround time 1hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1 interference Other surface antigens
TOTAL BILIRUBIN
Test information This is an estimate of total bilirubin and hence the severity of jaundice.
primary sample volumes 2 mL
special precautions nil
turnaround time 1hr biological reference intervals Refer Appendix 1
Test information This is the amount of protein in plasma. This is decreased in conditions like malnutrition, liver failure or cirrhosis, nephritic syndrome, this elevated in conditions like multiple myeloma.
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Test information This is the level of the harmone T3. Mostly it is protein bound primary sample volumes 2 mL
special precautions nil
turnaround time 1hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference Reverse T3
T4
Test information This is the level of the harmone T4. Mostly it is protein bound primary sample volumes 2 mL
special precautions nil
turnaround time 1hr biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference Reverse T3
TSH
Test information Assess thyroid function and pituitary function primary sample volumes 2 mL
special precautions nil
turnaround time 1hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference FSH, LH
TESTO STERONE
Test information Male sex hormone primary sample volumes 2 mL
special precautions nil
turnaround time 1hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1 interference Other steroids
UREA
Test information This is the end product of protein metabolism. Produced in liver and excreted by kidney mainly and also GIT. The level are elevated in kidney diseases, diarrhea, vomiting, dehydration etc
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URIC ACID
Test information This is the end product of purine metabolism. This is increase due to increased production due to some enzyme disorders and high cell turn around condition. This also increased in renal failure and alcoholism. This causes gout.
primary sample volumes 1mL
special precautions nil
turnaround time 1hr biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference Gentisic acid, hemoglobin
VIT D 25OH
Test information Vitamin D status primary sample volumes 2 mL
special precautions nil
turnaround time 1hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference Other steroids
VIT-B12
Test information Vitamin B12 status primary sample volumes 2 mL
special precautions nil
turnaround time 1hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1 interference Other corinoids
Hematology ABSOLUTE EOSINOHPIL COUNT (AEC)
Test information For the diagnosis of allergy primary sample volumes 2 mL
special precautions nil
turnaround time 1hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference neutrophils
AFB
Test information Acid fast bacilli, to diagnose tuberculosis
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primary sample volumes 2 mL special precautions nil
turnaround time 1hr
biological reference intervals Refer Appendix 1
clinical decision values Refer Appendix 1
interference Lepro bacili
BENCE JOHNS PROTEIN
Test information Marker for multiple myeloma primary sample volumes 2 mL
Female 1 Days 150 Years Infant ( 0 days - < 3 days ) : 2.0 - 5.0 Infant ( 3 days - 30 days ) : 0.9 - 2.2 Child ( 1 months - 18 yrs ) : 0.8 - 2.0 Adult ( 19 yrs - 150 yrs ) : 0.78 - 2.19
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Male 1 Days 150 Years Infant ( 0 days - < 3 days ) : 2.0 - 5.0 Infant ( 3 days - 30 days ) : 0.9 - 2.2 Child ( 1 months - 18 yrs ) : 0.8 - 2.0 Adult ( 19 yrs - 150 yrs ) : 0.78 - 2.19
FREE TRIIODOTHYRONINE (FT3)
Female 1 Days 150 Years 1 Day - 1 Year : 2.80 - 5.07 1 Years - 5 Years : 2.47 - 4.68 6 Years - 10 years : 2.67 - 4.62 11 Years - 14 Years : 2.02 - 4.30 15 Years - 150Years : 1.71 - 3.71
Male 1 Days 150 Years 1 Day - 1 Year : 2.80 - 5.07 1 Years - 5 Years : 2.47 - 4.68 6 Years - 10 years : 2.67 - 4.62 11 Years - 14 Years : 2.02 - 4.30 15 Years - 150Years : 1.71 - 3.71
G6PD
GAMMA GT
Female 1 Days 150 Years [ 12 - 43 ]
Male 1 Days 150 Years [ 15 - 73 ]
GLOBULIN - SERUM*
Female 1 Days 150 Years [ 2.3 - 3.5 ]
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Male 1 Days 150 Years [ 2.3 - 3.5 ]
GLOMERULAR FILTRATION RATE ( GFR )
GLUCOSE CHALLENGE TEST
GLUCOSE CHALLENGE TEST
GCT URINE SUGAR
GLUCOSE CHALLENGE TEST With 50 gm glucose
PLASMA GLUCOSE ( 1 Hr.)
URINE SUGAR (1 Hr.)
PLASMA GLUCOSE ( 2 Hr.)
URINE SUGAR (2 Hr.)
GLUCOSE CHALLENGE TEST With 75 gm glucose
PLASMA GLUCOSE ( 1 Hr.)
URINE SUGAR (1 Hr.)
PLASMA GLUCOSE ( 2 Hr.)
URINE SUGAR (2 Hr.)
GLUCOSE FLUID
GLUCOSE PLASMA FASTING WITH URINE SUGAR
GLUCOSE PLASMA FASTING *
Female 1 Days 150 Years [ 70 - 110 ]
Male 1 Days 150 Years [ 70 - 110 ]
FASTING URINE SUGAR
GLUCOSE PLASMA POST PRANDIAL ( AFTER BREAKFAST )
GLUCOSE PLASMA POST PRANDIAL ( AFTER BREAKFAST )
Female 1 Days 150 Years [80 - 160]
Male 1 Days 150 Years [80 - 160]
POST PRANDIAL URINE SUGAR (AFTER BREAKFAST)
GLUCOSE PLASMA POST PRANDIAL ( AFTER DINNER )
GLUCOSE PLASMA POST PRANDIAL ( AFTER DINNER )
Female 1 Days 150 Years [80 - 160 ]
Male 1 Days 150 Years [80 - 160 ]
POST PRANDIAL URINE SUGAR ( AFTER DINNER )
GLUCOSE PLASMA POST PRANDIAL ( AFTER LUNCH )
GLUCOSE PLASMA POST PRANDIAL ( AFTER LUNCH )
Female 1 Days 150 Years [ 80 - 160 ]
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Male 1 Days 150 Years [ 80 - 160 ]
POST PRANDIAL URINE SUGAR ( AFTER LUNCH )
GLUCOSE PLASMA POST PRANDIAL WITH URINE SUGAR
GLUCOSE PLASMA POST PRANDIAL*
Female 1 Days 150 Years [ 80 - 160 ]
Male 1 Days 150 Years [ 80 - 160 ]
POST PRANDIAL URINE SUGAR
GLUCOSE PLASMA POST PRANDIAL*
Female 1 Days 150 Years [ 80 - 160 ]
Male 1 Days 150 Years [ 80 - 160 ]
POST PRANDIAL URINE SUGAR
GLUCOSE PLASMA RANDOM *
Female 1 Days 150 Years [ 70 - 140 ]
Male 1 Days 150 Years [ 70 - 140 ]
RANDOM URINE SUGAR
GLUCOSE TOLERANCE TEST With 100 gm Glucose
PLASMA GLUCOSE (FASTING)
URINE SUGAR (F)
PLASMA GLUCOSE ( 1/2 Hr.)
URINE SUGAR (1/2 Hr.)
PLASMA GLUCOSE ( 1 Hr.)
URINE SUGAR (1 Hr.)
PLASMA GLUCOSE ( 1 1/2 Hr.)
URINE SUGAR (1 1/2 Hr.)
PLASMA GLUCOSE ( 2 Hr.)
URINE SUGAR (2 Hr.)
PLASMA GLUCOSE ( 2 1/2 Hr.)
URINE SUGAR (2 1/2 Hr.)
PLASMA GLUCOSE ( 3 Hr.)
URINE SUGAR (3 Hr.)
PLASMA GLUCOSE ( 3 1/2 Hr.)
URINE SUGAR (3 1/2 Hr.)
GLUCOSE TOLERANCE TEST With 75 gm Glucose
PLASMA GLUCOSE (FASTING)
URINE SUGAR (F)
PLASMA GLUCOSE ( 1/2 Hr.)
URINE SUGAR (1/2 Hr.)
PLASMA GLUCOSE ( 1 Hr.)
URINE SUGAR (1 Hr.)
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