HERD project: STUDY OF THE MICROBIOLOGICAL FLORA OF MILK ... · HERD project: STUDY OF THE MICROBIOLOGICAL FLORA OF MILK AND DAIRY PRODUCTS IN KOSOVO The microbial flora (quality)

HERD project:

STUDY OF THE MICROBIOLOGICAL FLORA OF MILK AND DAIRY PRODUCTS IN KOSOVO

The microbial flora (quality) of (i) milk and (ii)dairy products in Kosovo: With emphasis on the dairy lactic acid

bacteria and the two pathogens Listeria monocytogenes and Staphylococcus aureus

Research Education

Information Farmers

and Company

Institutions and People Involved University of Pristina: • Ibrahim Mehmeti, Post. Doc. • Skender Muji, Professor • Hysen Bytyqi, Professor • 2 Master student (right now)

– Selami Salihu (Master) – Ibadete Krasniqi (Master) – Imer Haziri (PhD student) 3 Bachelor student (Diploma for Bachelor) Norwegian University of Life Sciences: Ingolf F. Nes, Professor Dzung Bao Diep, Professor Technical Support

EDUCATION

Master student

• Ibadete Krasniqi (UP- Pristina Kosovo) • Selami Salihu (Agriculture University - Tirana

Albania)

Bachelor student

1. Remzi Jetullahu 1. Valdet Thaqi

2. Idriz Lalloshi …

Work Training at LMG-UMB

1.Imer Haziri (PhD student) 1.Ibadete Krasniqi Master student

Laboratory Manuel

• Microbiology and food microbiology

– Agriculture Faculty and Veterinary used for students, farmers and company

RESEARCH

RESULTS

I. Isolation and characterization of bacteria in milk obtained from

producers

Sampling of milk for bacterial analysis

Communities: 10 Farms: 237 Number of samples: 711 (3 tomes from each farm) In December-February (237 samples) In March- April (237 samples) In May-June (237 samples) Bacterial isolates: 5000

Samples collection

Podujeva

Istogu

Skenderaj

Istogu

Rahoveci

Gjakova

Theranda

Ferizaji

Sharri

Sharri

Skenderaj

Lipjanë

Rahovec

Sharri

Total Viable Count

4,99 6,33 9,42

7,6 6,91 5,48 5,88 5,49 6,47 5,14

35,29

0

5

10

15

20

25

30

35

< 1… 100 000 < 100 001… 200 000

< 200 001-300 000

< 300 001… 400 000

< 400 001… 500 000

< 500 001… 600 000

< 600 001… 700 000

< 700 001… 800 000

< 800 001… 900 000

< 900 001… 1000 000

< 1 000 000...

TVC /mL

28% complyies with the regulation

Analyses

• 5000 different strains are collected – 3000 Analyses in Norway – 888 are analyzed for biochemical testes:

– Growth at different pH (6.0 and 9.2) – Growth at different NaCl comcentration (2% and 6.4%) – Growth in two different mediums: MRS and GM17 – Gas production(homo-fermentative and hetero-fermentative)

– 888 LAB for bacteriocin production – Multi - Locus – Sequencing -Typing (MLST)?

Classification

Present status of 3000 isolates: • Staphylococcus aureus: 235 isolates • Listeria spp. 112 isolates • Lactic acid bacteria: 2653

Meeting and visits in farms

• Farms – Higher number (more contaminations) – Between higher and normal – Low number (according to regulation)

– Milk in company

Higher number of microorganisms

• Bad hygiene;

• No pipline desinfection;

• Hygiene of the farms;

Between extreme and limits

Good quality

Good hygiene

Good ventilation

Pipeline in good conditions

Bacteriocins activity

• 576 samples are tested; • 10 % are positive • Indicators:

– Staphilococcus aureus – Listeria innocua – Bacillus cerus – Lactococcus lactis – Lactobacillus sakei

Presentations

• Week Science in Kosovo – Two oral presentations:

– Ibrahim Mehmeti and – Ibadete Krasniqi

– Conference in AlbShkenca organized in Macedonia

– Ibadete Krasniqi oral presentation and Poster

Future work

Samples collection company

Podujeva

Istogu

Skenderaj

Istogu

Rahoveci

Gjakova

Theranda

Ferizaji

Sharri

Sharri

Skenderaj

Lipjanë

Rahovec

Sharri

Analysis of dairy products

Workshops

• Two workshops next summer organized with farmers and company;

• Results presentation; • Gust from Norway and Kosovo

Equipments

Thank you for your attention