Herbal drug and herbal mediated silver nanoparticles by akshay kakde

“Herbal drug and herbal mediated silvernanoparticles as anti-diabetics: A newhorizon”

Presented by: Mr. Akshay G. Kakde

M. Pharm Sem-III

Quality Assurance Technique

Guide : Dr. Santosh S. Bhujbal Co-Guide : Dr. Rabindra K. Nanda

Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune:411018.

1

“Herbal drug and herbal mediated silvernanoparticles as anti-diabetics: A newhorizon”

Author - Shanker kalakotla, Sandhya Rani, Pravallika P L.

Centre for Pharmaceutical Sciences, Institute of Science and Technology,

JNT University Hyderabad, Kukatpally, Telangana, India.

Publishing Journal - International Journal of Pharmaceutical

Sciences Review and Research.

Indexing – SciVerse Scopus , Index Copernicus, Google scholar

Impact factor - 2.5

Date of publishing - April 2015. 2

Abstract

• Objective – To study the synthesis of silvernanoparticles in treatment of diabetes.

• Method – Green synthesis method use to formulateherbal mediated silver nano particles was discussed.

• Result - Author concluded study by suggestingsynthesis & various characterization method ofHMSNPs(Herbal Mediated Silver Nanoparticles) &their pharmacological application to treat thediabetes.

• Conclusion - Synthesized and formulated herbalmediated silver nanoparticles are biodegradable,biocompatible and non-toxic and has greater abilityto enter biological cell membrane.

3

Introduction

• Diabetes is metabolic disorder caused by dysfunction of β

cell of pancreas lead to ↓ in production of the hormone

insulin or ↑ resistance to the action of insulin in peripheral

tissues.

• It categorized into 2 types. Type 1 diabetes develops when

the body’s errant immune system damages and destroys the

pancreatic β cell that produce the blood glucose regulating

hormone insulin.

• Type 2 diabetes is cause due to progressive insulin

resistance/ insensitiveness with an ensuing reduction in the

ability of the pancreatic hormone to suppress hepatic glucose

output.

4

What is Nanotechnology

• It is defined as the “International design, characterization

and production of materials, structures and system by

controlling their size and shape in the nano scale ranging

from 1-100 nm.”

• Nanoparticles are similar to biological molecule and

system so, they are potentially useful for medical

application.

• It show the completely new or improved properties such

as size, distribution and morphology of the particles.

5

Why nanoparticles unique from normalsize particles?

It has specific surface area, size and shape due to there

unique physicochemical properties.

Nano crystalline silver particles have been providing

application in filed of high sensitivity bio molecular

detection, diagnostics, antimicrobials therapeutics.

Silver has good inhibitory action to the microbial strain

against bacteria present in medical and industrial process.

The present scenario especially the SNP’s have been playing

important role in herbal nano technology to prevent chronic

diseases like diabetes and cancer.

6

Why Silver ??

Pure silver has highest

electrical and thermal

conductivity of all metals

and the lowest contact

resistance.

7

Metallic silver itself

insoluble in water, but

metallic salts such as AgNo3

& Silver chloride are soluble

in water (WHO 2002).

Need of green synthesis

The need for synthesis of nanoparticles due the

physical and chemical processes are costly.

Chemical synthesis method leads to presence of

some of toxic chemicals absorbed on the surface

that they have adverse effect in medical application.

Green synthesis provides advancement over

chemical and physical method as it is cost effective,

environment friendly, easily scaled up for large

scale synthesis.

In this method there is no need to use high pressure,

energy, temperature and toxic chemicals.8

Synthesis of HMSNP’s

The NPs can synthesized using various methods.

Among them chemical method required short time

period and quantity of nanoparticles

The ↑ in demand of “Green nanotechnology”.

Plant extract provide good effect with nanoparticles

and free from toxic chemicals.

9

Synthesis of HMSNPs

HMSNPs

Physical methodChemical

methodBiological

method

1) Evaporation-

Condensation

2)Laser

ablation

1) Sodium

borohydride

2) Ascorbic acid

3) Citric acid

1)Bacteria

2)Fungi

3)Plant

10

SNPs by chemical method

11

Green Synthesis of Silver Nanoparticle

12

Method for synthesis of SilverNanoparticles

• Procedure for preparation of SNP’s.

13

Green Synthesis of Silver Nanoparticle

14

Silver nanoparticles

The main advantage of using plant extract for silvernanoparticle synthesis is easily available, safe andnontoxic and have a broad variety in that can aid inthe reduction of silver ions.

The reduction of silver ions due to phytochemicals.

The main phytochemicals involved are terpenoids,flavones, ketones, aldehydes, amides and carboxylicacid.

Flavones, organic acids and Quinone are watersoluble phytochemicals that are responsible forimmediate reduction of the ions.

15

Images of Silver Nanoparticles

Fig (A & B) : AFM images of silver nanoparticles.

Fig (C): TEM image of silver nanoparticles.

16

Application of silver nano particles

NP’s are of great interest due to their extremely

small size and large surface.

NP’s leads to both chemical and physical

differences in their properties compared to bulk of

the same chemical composition, such as mechanical,

biological and steric properties, catalytic activity,

thermal and electrical conductivity, optical

absorption and melting point.

Designing and production of materials with novel

applications can be results by controlling shape and

size at nanometer scale.

17

Application of HMSNP’s

Herbal mediated silver

nanoparticles

(HMSNP’s)

Biotechn

ology/Mi

crobiolic

al

Medical Physical

/Optical

HMSNPs used as

antimicrobial/ anti

bacterial

HMSNPs used to

treat Diabetes,

cancer etc.

Emission

scattering and

spectroscopy18

Conclusion

HMSNPs : Scope of future research.

It is safe, non toxic, save time and economical.

It is also having high efficacy and therapeutic

effect.

19

References

• Cooke DW, Plotnick L. Type 1 diabetes mellitus inpediatrics. Pediatrics Revisions, 29, 2008, 374-384.

• Terminology for nanomaterials. Publicly availablespecification136. London: British Standards Institute,2007.

• Kaviya S, S.J., Viswanathan B., “Green Synthesis ofsilvernanoparticles using Polyalthia longifolia Leafextract along with Dsorbitol.”Journal ofnanotechnology, 3, 2011, 1-5.

• Chen X, S.H.J., “Nanosilver: Nanoproducts in MedicalApplications”. Toxicology Letters, 176, 2008, 1-12.

20

• Garima Singhal, R.B., Kunal Kasariya, Ashish Ranjan

Sharma,Rajendra Pal Singh, “Biosynthesis of silver

nanoparticles using Ocimum sanctum (Tulsi) leaf extract and

screening its antimicrobialactivity”. Journal of Nanoparticle

Research, 13, 2011, 2981-2988.

• Sastry M, Khan MI, Kumar R., “Microbial nanoparticle

production in Nanobiotechnology.” Nano biotechnology,

85(2), 2003, 163-169.

• Elumalai EK, P.T., Hemachandran J, Vivivan Therasa S,

Thirumalai T, David E., “Extracellular synthesis of silver

nanoparticles using leaves of Euphorbia hirta and their

antibacterial activities.” Journal of Pharmaceutical Sciences,

2(9), 2010, 549-554.

21

Thank you …….!!!!!

22