HER2 IEDC Presentation.pptx

8/18/2019 HER2 IEDC Presentation.pptx

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HER2 PROTEIN BIOSENSOR

DEPARTMENT OF ELECTRONICS AND TELECOMMUNICATION ENGINEERING

TRIDENT ACADEMY OF TECHNOLOGY,BHUBANESWAR

Project Guie - S! M" A#i $A%%t" Pro&" '

Grou( Me)*er%

Devee Ray

M. Spandana

Laxmipriya Bhatta Itishree Pattanaik


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HER2 PROTEIN BIOMARKER

•HER2 is a protein that stimulates the growth of reast!an!er !ells" It !an e foun# in $our loo# an# urine"%ometimes it&s referre# to as a 'tumor mar(er")

•Human Epi#ermal *rowth +a!tor Re!eptor 2 ,HER2 is

o.er e/presse# in 20-10 of reast !an!ers is a (e$pre#i!ti.e iomar(er an# an e3e!ti.e therapeuti!treatment target for reast !an!er"

•Normal in#i.i#uals ha.e serum le.els etween 2 an#

45 ng6ml an# reast !an!er patients ha.e le.els from45 to 75 ng6ml

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4/25/16 HER2 PROTEIN BIOSENSOR 3


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ABOUT THE PROJECT

8e.elop a sensor that will help to #ete!tthe patient&s loo# sample at reasonale!ost an# minimal e/pertise "

The goal of the pro9e!t is to #e.elop an#

#ete!t the HER2-E:8 an# resour!es thatwill help to support e3orts to re#u!e thee3e!t of HER2-E:8 responsile for reast!an!er #eaths an# unne!essar$ iops$ .

%pe!i;!all$< the pro9e!t is #esigne# to

a##ress the signi;!ant nee#s for impro.ingthe appli!ation for #ete!tion of HER2-E:8responsile for reast !an!er"



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EXISTING TECHNOLOGY

The most e/tensi.el$ use# metho# forHER2 e/pression anal$sis is ase# onImmunohisto!hemistr$ ,IH:"

A +luores!en!e in situ Hybridization(FISH) tecno!o"y is !urrentl$ use# to#ete!t HER2 gene ampli;!ation when

the IH: ase# results are un!lear"



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8E%:RIPTION

•

In or#er to #ete!t the presen!eof Her2 iomar(er the input isgi.en to the re!eption elementas sample anal$te"

• The re!ognition element!omprises of re!eptors su!h asantigens< antio#ies< en=$mes<

proteins< nu!lei! a!i#s" Thesere!ognition elements help in the#ete!tion of these anal$sts $the help of sensors"

• The sensors use# here is in theform of a!ousti! wa.es whi!h is

prepare# in the !omsol multi-ph$si!s software an# thus gi.esthe output in the form ofen#ing"



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%A>


•%urfa!e A!ousti! >a.e ,%A>is the general name for thewa.es that propagate at thesurfa!e of soli#s< whose#ispla!ement amplitu#esun#ergo e/ponential #e!a$eneath this surfa!e< similar to

the ones that propagate on thewater surfa!e" It has thepropert$ to generate ele!tri!alsignal in response to someinput ?uantit$

•%A> pla$s an important rolethat !an !ouple with an$ me#iain !onta!t with the surfa!e ofthe #e.i!e" This !ouplingstrongl$ a3e!ts the amplitu#ean# .elo!it$ of the %A> so that

it allows the %A> #e.i!e to#ete!t the !hange of input


8/254/25/16 HER2 PROTEIN BIOSENSOR 8

•%A> !an e e/!ite# an# #ete!te# e@!ientl$ $ using!om-li(e ele!tro#es name# I8T pla!e# on the

surfa!e of a pie=oele!tri! sustrate" The I8T!omprises two sets of interpenetrating metalli!ele!tro#es fari!ate# photolithographi!all$ on thesurfa!e of a pie=oele!tri! sustrate< one a!t as inputtrans#u!er< the other a!ts as output trans#u!er to!on.ert the %A> a!( to the ele!tri!al #omain"

• An acoustic #a$e #i!! be "enerated in te

substrate %Te #a$e!en"t o& te acoustic #a$e is

e'ua! to te distance etween two a#9a!ent I8T pairs

d since te #a$e!en"t is dened as te distance

bet#een consecuti$e corres*ondin" *oints o& te

sa+e *ase , tat is

-.d


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>ORKIN*

We are developing a SAW model sing

!"MS"L so#t$are.

A#ter this% $e $ill prepare a demo model $hereinpt valesre'en(y)*++ M,- o/tained

#rom an I000 resear(h paper $ill /e given and

the (orresponding otpt $ill /e #ond.

The model o# the sensor $ill /e #a/ri(ated

sing I12P program in IIS( Bangalore



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/ESI01 OF HAR/2ARE



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MATLAB PROGRAM



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%oftware se#



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Pro9e!t 8e.elopment Pro!ess

The pro9e!t #e.elopment pro!ess in.ol.esthe following pro!essesC

4" Diterature sur.e$"

2" Knowle#ge an# resear!h o.er !omsol

software"

1" 8esign of %A> sensor mo#el

" %imulation of %A> sensor

5" Result anal$sis



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Appli!ation Of Pro9e!t

IN ME8I:AD AN8 BIODO*FC4" 8ete!tion of 8NA an# proteins

2" 8ete!tion of m$ogloin

1" 8ete!tion of !ells an# h$ri#i=ation

" 8ete!tion of loo# glu!ose monitoring

. IN :HEMI%TRF AN8 ENGIRONMENTAD MONTORIN*C

4" 8ete!tion of temperature an# heat !hanges"

2" 8ete!tion of humi#it$"

1" 8ete!tion of infrare# light an# G ra#iations"" To !ount numer of a!teria in air

5" 8ete!tion of !hange in ph or salt !on!entration

" To #ete!t !on!entration of heri!i#es"



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%E+DNE%% O+ PROE:T

4" HER2 has emerge# as one of the est #iagnosti!

mar(er for the #iagnosis of most fre?uent !an!er in

woman i"e" reast !an!er"

2" The %A> sensor o3ers ease of use safet$< !omfort

an# !on.enien!e an# !ontriute to the !reation ofan e!o-frien#l$< !omfortale an# safe future"

1" A!hie.es spa!e sa.ing an# low energ$ !onsumption

lea#ing to the !reation of a resour!e- sa.ingen.ironment"

" The sensor use# is of High ?ualit$"



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RE%DT ANADF%I%


GEOMETRY OF SAW SENSOR MESH ANALYSIS OF SAW SENSOR


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Result An# Anal$sis ,!ont"


F!"E!#$ %'( )* *+&%,-# .*,-##$ +*$! A+$+$&.#


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%:OPE O+:OMMER:IADIJATION

1( COST EFFECTIE

2( AAILABILITY

3( EFFICIENT

4( MLTI TILITY



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+TRE >ORK

•

+uture resear!h shoul# e #ire!te# towar#simpro.ising the merits of these #iagnosti!platforms for !lini!al #iagnosis of reast!an!er to ring an e3e!ti.e !hange in themanagement of reast !an!er patients"

• The %A> sensor #esigne# also !an e use# inthe #ete!tion of other !an!er ase# #iseasesan# along with the !an!er #iseaseprogression"

• The sensor !an also e use# in the #ete!tionof atomi! or nu!lear oms $ the help of gassensors"



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Re&ere+ce%

4/25/16HER2 PROTEIN BIOSENSOR

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4L un Jou 8ept" of Ele!tr" :omput" Eng"< Illinois ni."< rana<ID< %A :hang Diu %!hutt-Aine inghong :hen %ung-Mo KangEle!tron 8e.i!es Meeting< 2000" IE8M 00"Te!hni!al 8igest

International"

2L i=heng*u>ispr$< In!"< Ir.ine< :A< %A 8e Duis< "R" %oli#-%tate an# Integrate# :ir!uit Te!hnolog$ ,I:%I:T< 2040 40th IEEEInternational :onferen!e on 4- No." 2040"

1L %R+A:E A:O%TI:% >AGE %EN%OR BF %IN*

PIEJOEDE:TRI: 8EGI:E% $ Ra(esh (umar Pati4 < %"("mohamme#ali4< %a(untala Mohapatra4< Millee Panigrahi"

L Dael-free #ete!tion of reast !an!er mar(er HER-26neu withan a!ousti! iosensor $ +rie#eri(e " *ruhlQ< Mi!hael Rapp<Kerstin Dange"

5L INTRO8:TION TO :OM%OD Multiph$si!s"

L Breast !an!er #ete!tion using a h$ri# +inite #i3eren!efre?uen!$ #omain an# parti!le swarm optimi=ation te!hni?ues $%" H" Jainu#-8een +a!" of Ele!tron" Eng"< Menou;a ni."< Menouf >" M" Hassen E" M" Ali K" H" Awa#alla"


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!"1!L2SI"1S

HER2 has emerge# as one of theest mar(er for the #iagnosis of most

fre?uent !an!er in woman i"e" reast

!an!er"

The %A> ase# iosensor promises

an a#.an!ement in the iomar(ersensing te!hnolog$ that is !ost

e@!ient an# repro#u!ile"



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T,$

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HER2 IEDC Presentation.pptx

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