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Hellenic Accreditation System
Annex F1/B15 to the Certificate No. 44-4
SCOPE of ACCREDITATIONof the
Analytical Laboratoryofthe
AGROLAB SA. (Laboratory in Athens)
Materials / ProductsTested
Types of Test / Properties Applied Standards /Techniques
Chemical Testing
1. Fruits andVegetables highwater content
(stone fruits, pomefruits, fruitingvegetables,citrusfruits, root-tubervegetables, stemvegetables, smallfruits, pulsesvegetables, brassicavegetables, bulbvegetables, leafvegetables and freshherbs,miscellaneous(including tropicfruits, as referred toRegulations(EQ396/2005 and(EQ187/2006)
Determination of AcrylamideIn house method UPLC-MS-M1.Steven J. Lehotay, KaterinaMastovska & Alan R.Lightfield: Journal of AOACINTERNATIONAL Vol. 88No2,2005
2., Katerina Mastovska &Steven J. Lehotay, Journal ofAgriculture and FoodChemistry, 2006, 54, 7001-7008.
Page 4 to 8 Annex F1/BI5 to the Certificate ESYD No 44-4 21.10.2013
Materials / ProductsTested
1. Foods and animal feed
2. Milk and dairyproducts
3.Milk and infant & babyfoods containing milk
4. Meat and MeatProducts
Types of Test / Properties
Microbiological Testing
1. Enumeration of total viable count
2. Coliforms (Enumeration)
3. E. coli (Enumeration)
4. Bacillus cereris (Enumeration)
5. Staphylococci coagulase positive Staphylococcus aureus and otherspecies (Enumeration))
6. Yeasts / Molds (Enumeration)
7. Yeasts / Molds (Enumeration)
8. Salmonella spp (detection)
9. Lisleria spp (detection)
10. L monocytogenes (detection)
1 1 . E.coli O157-.H7 (detection)
1 2. Slaphyhcoccal enterotoxin (detection)
1 3. Entero bacteriaceae (Enumeration)
14. C.perjringens (enumeration)
1 5. Anaerobic sulfite reducing bacteria and clostridia (enumeration)
5. Enumeration of culiurable microorganisms at 37 and 22 °C
6. Coliforms (Enumeration)
7. Faecal Coliforms (Enumeration)
8. E.Coli (KaTct|i£Tpr]an.)
9. Enumeration of Escherichia coli and coliform bacteria
10. Intestinal enterococci (Enumeration)
1 1 . Detection and enumeration of Cl. perfringens (including spores)
1 . Enumeration of culturable microorganisms at 37 and 22 °C
2. Coliforms (Enumeration)
3. Faecal Coliforms (Enumeration)
4. E.Coli (Kara|ieTpr|cm,)
5. Intestinal enterococci (Enumeration)
1. Detection and enumeration of intestinal enterococci
Enumeration of total cultutabie microorganisms at 20 °C (± 2°C)
Applied Standard^/Techniques
AFNORBKR 23/07-10/11
ISO 6579:2002/Amd 1 :2007,ANNEX D
White Kauffhiann Le MinorScheme 2007, 9n EKooon
ISO 6461-2:1986ISO 19250:2010
15011731:1998
ISO 11731-2:2004
ENASO 16266:2006
ISO 6222: 1999
ISO 9308-1:2000
APHA 9222D: 2005
ISO 9308-1 :2000/cor 2007
AFNORBRD 07/20-03/11
ISO 7899-2: 2000
Annex III of J.M.D. Y2/2600/2001,as modified, completed and validuntil nowISO 6222: 1999
ISO 9308-1:2000
APHA 9222D: 2005
ISO 9308-1 :2000/cor 2007
ISO 7899-2: 2000
ISO 7899-2:2000
ISO 13959:2009
Page 6 to 8 Annex F1/BI5 to the Certificate ESYDNo 44-4 21.10.2013
Materials / ProductsTested
Types of Test/ Properties Applied Standards /Techniques
Sampling
1. Samples from surfacesusing swabs and contactplates
1. Horizontal methods for sampling techniques for microbiologytests
ISO 18593:2004
Biological tests
1. Cotton (seeds) Detection of GMO (detection of CMV 35S promoter,terminator, PAT gene, BAR gene, and element CTP2-CP4EPSPS)
NOSInternal method OB.04.611 basedon:1.ISO 21569:20052. Gaudron et al., Eur. Food ResTechnol, 229: 295-305,20093. Grohmann et at., J. Agric FoodChem, 57: 8913-8920,20094. Sebah et al., Project GMOseek,Development of screening methodsfor GMOs, Final Report, 20105. Kodama et. al., Journal of AOACInternational vol. 92, No. 1,20096. Macherey-Nagel, NucleoSpinFood kitusing Real-Time PCR
2. Potato and potatoproducts (food, feed, rawmaterials)
Detection of amylopectin potato event EH 92-527-1Internal method OB.04.6112based on1. 15021569:20052. Event-specific Method for theQuantification of AmylopectinPotato Event EH92-527-I UsingReal-time PCR, Protocol EH92-527-1-Community ReferenceLaboratory for GM Food and Feed,CRLVL09/05VP3. Macherey-Nagel, NucleoSpinFood kit.using Real-Time PCR
3. Rice and rice products(food, feed, raw materials)
Detection of Rice
Line LLRICE 62
GM-eventLL601
Bt 63 Rice
Internal method OB.04.6113based on1.15021569:20052. Event-specific method for thequantification of rice line LLRICE62 using Real Time PCR (EventProtocol LLRICE62 - Communityreference laboratory for GM Foodand Feed)3. Multiplex real time PCR for thesimultaneous detection andquantification of DNA from threetransgenic rice species andconstruction and application of anartificial oligonucleotide as referencemolecule. R. Koppel, F. Zimmerli &A. Breitenmoser, Eur. Food ResTechnol (2010) 230:731-7364. Report on the validation of anevent-specific method for thedetection method foridentification of Rice GM-eventLLRICE 601 using a Real TimePCR assay. CommunityReference Laboratory for GMFood and Feed
Page 7 to 8 Annex F I / B I S to the Certificate ESYD No 44-4 21.10.2013
Materials/ProductsTested
Types of Test / Properties Applied Standards /Techniques
Rice and rice products(food, feed, raw materials)
(continue)
5. Report on the verification ofthe performance of a method forthe detection of Bt 63 Rice usinga real time PCR assay.Community Reference Laboratoryfor GM Food and Feed. CRL-EM-02/06, verification report RiceBt636. Macherey-Nagel, NucleoSpinFood kitusing Real Time PCR
4. Soya and soya products(seeds, food, feed, rawmaterials)
Detection of GMO (CMV 35$ promoter, NOS terminator, PAT gene,BAR gene, CTP2-CP4EPSPS element and S-adenosy!-L-methioninesynthesase promoter)
Internal method OB.04.611 basedon:1.15021569:20052. Gaudron et al., Eur. Food ResTechnol, 229: 295-305,20093. Grohmann et al., J. Agric FoodChem, 57: 8913-8920,20094. Sebah et al., Project GMOseek,Development of screening methodsfor GMOs, Final Report, 20105. Macherey-Nagel, NucleoSpinFood kit6. C. Bahrdt, et al., Anal BioanalChem 396:2103-2112,20107. Kodama et. al., Journal of AOACInternational vol. 92, No. 1, 2009
using Real-Time PCR
5. Corn and com products(seeds, food, feed, rawmaterials)
Detection of GMO (CMV 35S promoter, NOS terminator, PAT gene,BAR gene and CTP2-CP4EPSPS element)
Internal method OB.04.611 basedon:1.15021569:20052. Gaudron et al., Eur, Food ResTechnol, 229: 295-305,20093. Grohmann et al., J. Agric FoodChem, 57: 8913-8920,20094. Sebah et al.. Project GMOseek,Development of screening methodsfor GMOs, Final Report, 20105. Kodama et. al., Journal of AOACInternational vol. 92, No. 1,20096. Macherey-Nagel, NucleoSpinFood kit
using Real-Time PCR
Site of assesement : Laboratory permanent premises, Industrial area, Markopoulo, AttikiApproved signatories: A. Giannousios, C. Alexandrou, KPapanotas, D.Koraki
This Scope of Accreditation replaces the previous one dated 1 .3.20 1 3The Accreditation Certificate No. 44-3 to EL£TOgp/lEC 1 7025:200^, is valid until 26.1 1 .2017.
Athens,, 21. 10.2013
atories Accreditation Division
Page 8 to 8 Annex F1/B15 to the Certificate ESYD No 44-4 21.10.2013