Heat Stable Bacillus Spores as Vaccines Professor Simon Cutting SporeGen Ltd. School of Biological Sciences Royal Holloway University of London Egham, Surrey TW20 0EX, UK [email protected]
Heat Stable Bacillus Spores as Vaccines
Professor Simon CuttingSporeGen Ltd.School of Biological SciencesRoyal Holloway University of LondonEgham, SurreyTW20 0EX, [email protected]
Ethical Non-pathogenic Storage Robust (heat-stable), suitable for 3rd World Cost Straightforward production Route Oral edible vaccine. Intra-nasal (killed spores)
Licensed OTC drug (Bacillus clausii)100m doses sold/yr in Italy by Sanofi-Aventis
Bacillus spores
The Concept of Spore Vaccines
Stomach
Spores survive intact
vegetative Bacillus killed
Small Intestine
Nutrient rich
Spore germination &
dissemination to GALTColon
Re-sporulation
GIT
Excretion of sporesinto the soil
Symbiotic interactions with plants
Consumption ofplants and spores
spores
vegetative cells
Bacillus as a gut commensal
NattoB. subtilis var. Natto
Japanese staple
BioPlus 2BSpores of B. subtilis & B.
licheniformisAnimal feed
Christian Hansen (Denmark)
Bacillus Spores: Probiotics, Functional Foods and Feed Supplements
Main Street Gourmet (USA)obtained from Isabellas Healthy Bakery
ActivateProbiotic muffins impregnated with spores
1.E+00
1.E+01
1.E+02
1.E+03
1.E+04
1.E+05
1.E+06
1.E+07
1.E+08
1.E+09
1.E+10
1 2 3 4 5 6 7 8
HU58
Protexin
Control
Before cooking
0 7 14 28 42 56 84
Days
Spor
es co
unt /
gra
m
Survival of spores incorporated in biscuits (McVities) after 84 days
These are background countsAnd are Bacillus spores present in your biscuit mix. The biscuit mix is not sterile and data shows that cereals, wheat etc have a basal level of spore counts.
Starting CFUHU58 Tube 1 + 1kg mix = 6.57 X 108 spores/gPXN21 Tube 1 + 1kg mix = 1.33 X 109 spores/g
235oC 8 min.
+Killed Spore
Killed Spore
Antigen(protein or virus)
Vaccinate
Expression on Spore CoatRecombinant (GMO)
Adsorption onto the Spore CoatNon-recombinant/non-GMO
Vaccinate
Spore gene X + Antigen gene Y
X Y
rec spore
SporeVax
Strengthening Animal Production and Health through the Immune Response
The European research project SAPHIR aims to develop innovative, safe , affordable and effective vaccine strategies effective against endemic pathogens responsible for high economic losses in livestock.
SporeGen is collaborating on the development of spores for 2nd generation oral/feed vaccines for Necrotic Enteritis
Shrimp Aquaculture(>$11bn/annum)
Penaeus monodonBlack Tiger Shrimp
Massive reduction in production to disease
Litopenaeus vannameiWhiteleg shrimp
Originally from Central/South AmericaIntroduced to Asia to replace P.
monodonBut stock now suffering epidemics of
disease
Shrimp Pathogens
About 30 viral diseases of shrimps White Spot Syndrome Virus (WSSV) is universally considered the worst
Expression on Spore CoatRecombinant (GMO)
Vaccinate
Spore gene X + VP28/VP26
X Y
rec spore
Spores display VP28 and VP26capsid proteins
Shrimps that eat the vaccine in feed pellets are protected against White Spot Syndrome Virus
Mortality rate (%)
Days post challenge
Protection trial: feeding during 14 days, then challenge
SPR at day 14 by CotB-VP28 at 1 x 10*9 cfu/g = (1-19.23/100)*100% = 81% SPR at day 14 by CotB-VP28 at 1 x 10*11 cfu/g = (1-10/100)*100% = 90%
0102030405060708090
100
1 2 3 4 5 6 7 8 9 10 11 12 13 14
UnchallengedUntreatedPY79 (1 x 10*9 cfu/g)CotB-VP28 (1 x 10*9 cfu/g)CotB-VP28H (1 x 10*11 cfu/g)
Does it work?
Phase 1/2a clinical trialRecruiting nowin Germany
www.cdvax.org
SporeGen (www.sporegen.com)
Operating in UK and Vietnam
SporeVax for development of human and animal vaccines
Spore mediated delivery of heat-stable feed enzymes (phytase, xylanase etc)
Clostridium difficilevaccinesnovel treatmentsdiagnostics
Helicobacter pyloritreatments
Animal feed productsHanoi, Vietnamhttp://biospring.com.vn
+ SGF + SIF
72 kDa
72 kDa
1 2 3 4
41 kDa
A Cytoplasmic expression (rrn0-GST-Cpa247-370)
B Spore coat expression (CotB-GST-Cpa247-370)
58 kDa
41 kDa
58 kDa
41 kDa
15 kDa
15 kDa
C. perfringens
A (Oral) B (Nasal)
Cpa (Clostridium perfringens) toxin A expressed on spores
3.6 g/dose; 2 X 1010 spores 0.15 g/dose; 2 X 109 spores
Seroconversion, IgG
Chart3
4040.214042.340.3023.216338907905.360206561822.97873957
406641.3112.461.2019.69300576513.400409163717.369686654413.6303805758
37.97354.9295.641222.954745831720.758675798123.4416478273119.4987208581206.7500835338
4040402911.74937.4004.0424422064985.13130851562556.0593300985
Nave
PY79
GST-
PY79+ GST-
HT251
Days post primary vaccination
End-point titer
test IgG, IgA
0.2930.0340.2420.080.1430.1030.3170.016
0.2080.2170.2720.170.0260.1050.1940.286
0.240.210.2750.2530.1620.1530.1830.198
0.140.1640.2380.2410.0430.0820.1270.197
0.2450.2070.2730.2560.1620.1710.2110.274
0.1930.1760.2430.2440.0310.0620.1240.197
0.2350.2070.2710.2590.1630.1660.2060.243
0.2150.1970.2680.2650.1580.190.2040.22
0.4740.3570.3540.4010.02
0.480.4060.4530.5060.023
0.5020.4760.5050.5070.468
0.5190.5020.5030.5060.518
0.3020.0490.1720.1230.019
0.4080.2050.2620.2890.025
0.4370.3430.3340.4250.435
0.4950.4680.4310.4640.181
17-Sep
IgA_266_Nnasallung-IgA
12-80.6050.3950.2910.1880.1480.1070.0570.0630.060.060.0770.077Blank
12-70.5910.4710.3630.2230.1710.1260.8630.8680.7390.330.4710.344Pos
12-60.7640.5820.4170.2620.1740.1250.260.2050.1360.0830.1080.101Py-4
12-50.6980.5480.4180.2850.1980.1290.2620.1730.1340.080.1050.095py-3
12-40.9420.9380.8480.6890.4810.30.2180.1490.1220.070.0980.086py-2
12-30.4020.350.240.1680.1180.0920.2660.1770.1290.070.0970.098py-1
12-20.3450.3010.20.1310.1010.0890.1990.1260.1170.0720.1080.102naive3
12-10.6820.4630.3160.2220.1440.1230.2260.1250.120.0780.1110.1naive2
IgG-266-NnavePy12-1
0.2550.1870.1610.1060.1820.1310.1660.1870.1560.140.1771.131
0.1480.090.0830.0640.1020.0720.1020.1080.0940.0840.1020.999
0.0960.0620.0580.0480.0760.0490.0750.0750.0740.0580.0670.896
0.0940.0470.0460.0380.0640.0470.0780.0550.0610.0480.0540.664
0.0760.0430.0390.0360.050.0370.0460.0440.0490.0410.0420.449
0.0810.0410.0360.0330.0420.0360.0410.0380.0430.0290.040.279
0.0750.0420.0360.0330.0390.0360.0370.0350.0370.0340.0370.173
ip-pos1.4761.4431.3371.2021.1341.0710.8980.7170.0610.0540.070.088
1/4001/51200
18-Sep
IgA_266_Nnasallung-IgA
12-80.7610.5010.3460.2240.1780.140.1180.1250.1250.1250.1190.127Blank
12-70.6910.4710.3420.2370.2230.1421.1231.0680.9730.8030.5750.418Pos
12-60.7320.6060.4150.2960.1960.1560.270.2210.1810.1490.1280.134Py-4
12-50.8120.6690.4790.360.2230.1690.2230.2040.1680.1480.1240.112py-2
12-41.0811.0340.9210.7720.4280.3730.2280.1970.1580.1380.1130.113py-2
12-30.4510.3470.2420.1850.1010.1140.3260.2490.2070.1720.1460.139py-1
12-20.3920.2630.1920.1470.0590.1020.3170.2620.2170.1740.1510.131py-3
12-10.8790.5030.3210.2320.1040.1460.7960.6120.3920.2640.2180.196naive2
IgG-266-Npy12-1-paper12-1-serum
0.2050.1560.1910.2230.2020.250.330.8740.210.1710.1580.8921/40
0.1410.0750.0980.1220.1330.2030.2610.7760.1340.0830.0930.8181/80
0.1010.0550.0650.0890.0950.1250.1630.730.0950.0610.0630.6891/160
0.0730.0430.0490.0670.0650.0810.1110.5710.0720.0450.0430.5171/320
0.0540.0360.0380.0490.0490.0630.0710.430.0490.0370.0360.3621/640
0.0460.030.0310.0410.0420.050.0530.2870.0410.0330.0320.2251/1280
0.0370.0290.0290.0320.0350.0330.0420.1460.0340.0260.0280.146
IP-pos0.9690.9910.8450.8080.7230.5630.4910.2650.0440.0440.0490.061
1/800102400
neutralisation
NaCL
11-SepHT251IPnasalORAL
0.3540.2980.310.2010.30.2280.180.1180.3370.2710.2050.0861
0.3750.3320.3030.2120.1310.0170.0170.020.3480.3250.3050.3142
0.2990.3320.2750.2140.4570.4410.3760.2490.0130.0160.020.0283
0.2650.1890.1560.0830.3180.2430.1680.10.3840.3070.30.2654
ip-Pos0.140.0390.0170.0150.2870.2670.2260.1790.310.2720.2590.2625
0.2950.2490.2520.1910.2650.2270.190.1520.3350.2950.2820.2396
0.3240.2910.2920.260.3110.2940.2750.2380.0210.0130.0170.0227
0.3020.2940.4290.0460.270.1550.0230.0310.2990.2180.1180.0318
toxintriton-XNaCL
HT266IPnasalORAL
0.3450.3220.2730.2410.2910.2570.1780.0790.4510.3510.2850.3021
0.3230.2870.2580.2760.3480.290.2670.2290.3860.3360.2930.2792
0.3420.3080.2710.2630.3010.2830.2420.2160.3620.3130.2650.2583
0.3020.2880.2650.2350.3010.2730.2210.1940.3640.3340.2890.264
0.3470.3420.310.2850.2850.2350.1610.0710.3440.3090.2910.2835
0.3520.3290.3110.3140.310.2930.2610.2650.3590.3170.2890.2886
ip-Pos0.2030.0940.0250.0190.2980.2620.2270.1780.3430.2780.2210.1367
0.2150.3380.4140.0180.3930.290.2750.2830.380.3580.3450.3128
toxintriton-XNaCL
HT230ORALnasalIPtriton-Xtoxin
80.3180.3530.3670.3950.2090.2790.3190.3190.3820.4450.5180.379
70.2980.3050.3210.3560.3070.3040.3360.3590.5290.3220.3640.383
60.2160.3110.3090.3340.2330.2510.2650.2880.5360.2950.3230.385
50.4770.3790.2010.2850.1670.1950.2470.2650.530.0260.0230.124ip-Pos
40.2820.3090.3270.3710.3080.3320.4470.3770.4660.2870.3330.334
30.30.3210.3720.3650.3320.3730.3960.3890.4520.2610.2910.314
20.2950.370.3330.360.4540.4640.4830.4160.4570.3120.3450.341
10.360.3630.3840.40.2680.2950.3430.3470.3730.3320.3150.445
protein
NaCL
ip-pos0.0250.0230.0650.1120.1680.4240.1030.080.0820.1840.2080.257
0.120.1830.2250.2250.0840.1840.2180.2250.120.1810.2150.282
0.2230.2270.260.2530.0670.1670.2240.3180.060.1310.20.235
0.1590.1870.2360.2650.0660.1650.2160.2680.1260.2090.2110.25
0.0890.190.230.2680.080.1880.2410.2920.1560.210.2420.268
0.1880.1710.2080.2340.0650.1570.2060.2790.1090.1510.180.245
0.0780.1450.2210.2550.0970.180.2290.2590.1030.1750.220.253
0.1680.1180.2280.2680.1220.1810.2380.2990.1260.1860.210.205
BORATE
12-SepHt266oralnasalboratetritonX
80.0970.1850.2430.2960.0960.1750.2660.3130.0340.040.3460.372
70.1230.1920.2730.3030.1220.1970.2720.310.290.3710.370.38toxin
60.0910.1650.2410.2920.1010.1840.2670.3140.3130.3380.3480.351IP (1/400)
50.0780.1840.2490.2840.0870.1650.2510.2980.1770.2690.3120.349Py_Nasal
40.1110.2070.2660.2910.1060.1890.2610.2970.1590.2660.2870.32
30.0830.1690.2370.2820.1060.1760.2440.2910.1470.2250.30.329PY_Oral
20.0830.1690.2420.2870.2320.2880.3010.3070.1720.2090.2720.309
10.1080.2340.2790.30.2670.3030.3130.2930.1410.2120.2770.31612_1
13-Sep
HT230oralnasal
80.2380.2730.3360.3840.2090.2760.3390.410.0340.0270.420.443
70.1990.2610.320.370.210.2790.3380.3970.3910.4490.4550.454
60.2260.2710.3230.3710.2280.2760.3280.3640.140.3040.3750.417toxin-pos
50.250.2650.3260.3290.1490.2340.2980.3540.2860.3510.3760.386py-2
40.2350.2810.3280.3650.2310.3180.3660.3880.2660.3370.3870.411py-1
30.2160.2560.3180.3720.2680.3280.3640.3880.1530.2070.30.371py-2
20.2270.250.2970.3550.3150.3640.3790.4090.2410.2840.3340.376py-1
10.160.2520.3140.360.2210.2630.2940.3890.2580.3290.3710.443nave
13-Sep
HT251oralnasalboratetritonX
80.0350.1180.220.2940.0320.1240.2380.3250.0210.0270.4180.433
70.0320.0280.0340.1340.1420.2040.2930.3360.3960.4040.3960.414
60.1340.2050.2740.3060.1090.1720.2560.3310.070.2410.3180.374
50.1380.20.2690.3080.1310.1980.2480.3460.230.290.3280.371py-2
40.1450.2170.260.3830.1480.2010.2590.3180.2160.2640.3190.369py-1
30.0390.0310.0560.190.2070.3150.330.3910.1160.1710.2460.325py-2
20.1260.1920.2560.2940.0250.1010.2160.310.2030.2350.2860.342py-1
10.1070.1880.2530.3010.1360.2070.2950.3640.2690.2640.3090.362nave
13-Sep
IPHT251boratetritonX
80.0890.220.2730.2980.140.1890.2480.2980.0210.0220.3490.372
70.1640.2140.2550.290.150.2060.2740.2950.3330.340.3390.352toxin
50.1570.180.2330.2640.1540.2180.270.3050.1070.2160.2820.325IP
40.1820.2180.2540.2760.1240.180.2330.2810.1520.2060.2580.302py-2
30.1620.1970.240.2650.1350.1850.2360.2720.1350.1930.2540.307py-1
20.1760.1960.2430.2720.130.1840.2370.2770.1710.2130.2640.306py-2
10.1890.2030.2410.270.0440.1060.1640.120.1460.1920.2480.294py-1
py-30.1920.2150.2620.2810.1350.1780.2290.2820.1850.2380.2760.315nave
HT266HT230
13-Sep
oralproteinPY+protein
80.1050.1870.2370.3120.0470.0330.0280.070.0260.0270.3670.382
70.2330.2790.3070.3580.220.2490.3010.340.3670.3720.370.372
60.1860.2150.2770.3380.210.3020.3540.3790.1380.2250.2970.34
50.1750.2320.290.3610.1770.2370.3040.3350.1660.2350.2820.322py5
40.1820.2270.2910.3350.1860.2610.2990.3230.180.230.2730.307py-4
30.1350.1840.2430.3070.1440.2130.2990.3210.1870.2450.3030.338Py3
20.1840.2240.280.3240.1570.2190.2770.3030.1020.1650.2390.286py-2
10.2120.2480.30.3250.1670.2610.2890.3190.2420.2840.310.33nave
14-Sep
nasalproteinpy+pronegatriton
80.2840.3510.4110.4090.3410.3990.4070.3430.0120.0130.4280.451
70.3490.4010.4210.4240.2190.330.4230.3240.4690.4690.4640.468toxin
60.2630.3540.4110.4160.0370.150.1990.1480.1960.3910.4410.463IP
50.1970.4010.4260.4130.2130.3190.3780.3050.3310.3870.4190.442
40.230.3870.4170.4070.2290.3360.3880.330.3780.4020.4120.434py-nasal
30.2630.3460.3940.4010.190.3030.3620.3660.3490.3850.4140.434
20.2520.3520.4010.3970.2230.3250.3690.360.3820.4190.4320.444
10.3190.3690.410.4060.1780.2670.3710.3380.2050.2610.3430.385nave
14-Sep
IPproteinpy-2py+pronegatriton
py-10.2720.3610.3960.4360.3420.4040.430.4490.0150.0140.4780.543
70.2630.3690.3880.4320.3370.3890.4020.4470.4980.5140.5130.504
60.2680.3640.3630.4390.4280.4660.4740.4780.1640.340.4490.462
50.2530.3420.3710.4460.3580.3640.3960.4380.3470.4080.4310.462py-6
40.2290.3130.3380.3840.2820.3470.3850.4210.2860.3410.4250.412py-5
30.2820.3660.3820.4120.3480.3130.3650.4110.290.3490.3910.433py-4
20.2420.340.3640.390.2670.3280.3580.3960.2630.3560.3950.434py-3
10.2350.3170.3370.3970.2660.2770.3360.3820.2160.2830.3430.384nave
ip-py
nave0.3180.3370.3630.3690.2010.270.3240.3540.040.0280.0320.15Py+protein
0.1480.2280.2850.3320.2010.2840.330.3730.3510.4240.3990.404toxin
80.2130.2770.3150.3470.2080.2990.3430.3580.3510.4010.3950.396
Py-70.2410.3340.3660.3710.2070.3170.360.3750.180.2260.30.341nave
oral-60.2520.3080.3410.3590.2020.2730.3180.3480.1870.2030.2640.31
50.2280.2830.3260.3480.2020.2610.3110.3320.030.0240.3720.378control
40.2550.2990.3360.3460.2770.3120.3330.3480.3310.3770.3710.369toxin
30.1370.2270.2910.3270.3050.3190.3390.3550.140.2610.3270.347pip-pos
nasal py
13-Sepcompare Na_borate
Nasal0.0420.3750.3690.3740.016na
IP0.1470.4160.3870.4020.466tri
Py0.1560.4050.380.3970.401toxin
nave0.1150.4030.4010.4110.411toxin
Nasal0.040.0570.040.0280.007na
IP0.0070.010.0090.010.337tri
Py0.2440.1050.0690.0410.214toxin
nave0.2270.1220.1990.3920.406toxin
natritoxintoxin
control0.0390.6720.6250.634
IP0.1320.3560.4860.571borate
nave0.340.5110.5610.593
py0.3040.3840.5210.599
control0.0320.6490.5770.57
IP0.0380.1670.3570.469nacl
nave0.3890.4710.5180.551
py0.3080.4210.5030.57
0.050.0680.10.0560.0840.1150.0580.0830.1080.0190.0160.02
0.040.0610.0880.070.10.1210.050.0790.1090.0250.0160.011
0.0370.0570.0880.0630.0720.1020.0640.0840.110.0230.0160.016
0.0380.0590.0880.0490.070.0930.0560.0830.1090.0220.0390.028
0.0540.0790.1090.0450.0660.090.0480.0690.0930.0210.0190.018
0.0430.0620.0930.0570.0760.1020.0660.0830.1070.020.2560.264
0.0440.0640.160.0460.0680.0960.0660.0880.1130.020.1920.192
0.0550.0790.1110.1230.1390.1680.050.070.0970.0180.0150.02
0.1960.2180.2360.20.1630.2520.2370.2640.2820.0210.0210.023
0.1860.1960.210.2330.2220.2520.1850.220.2430.0230.0180.022
0.1630.1830.2040.2070.1930.2460.2480.2740.2810.0190.0340.033
0.1460.1650.1890.1630.1490.2190.2030.2320.2440.0220.1350.129
0.1760.1890.20.150.1260.1980.1650.2010.2140.020.0210.023
0.1490.1760.1930.2090.1690.2290.2290.2350.2390.0210.2590.276
0.1550.1770.2410.1740.1450.190.2250.2430.2480.0170.3010.299
0.190.2070.2250.2620.1950.2930.1990.2260.2310.0180.0290.028
lung_IgA
LUNGIgA
17-Sep
IgA_266_Nnasal
12-80.6050.3950.2910.1880.1480.1070.0570.0630.060.060.0770.077Blank
12-70.5910.4710.3630.2230.1710.1260.8630.8680.7390.330.4710.344Pos
12-60.7640.5820.4170.2620.1740.1250.260.2050.1360.0830.1080.101Py-4
12-50.6980.5480.4180.2850.1980.1290.2620.1730.1340.080.1050.095py-3
12-40.9420.9380.8480.6890.4810.30.2180.1490.1220.070.0980.086py-2
12-30.4020.350.240.1680.1180.0920.2660.1770.1290.070.0970.098py-1
12-20.3450.3010.20.1310.1010.0890.1990.1260.1170.0720.1080.102naive3
12-10.6820.4630.3160.2220.1440.1230.2260.1250.120.0780.1110.1naive2
19-Sep
plate-1HT230PY+pro
oral0.5410.3890.3150.2160.1740.1470.8580.850.8470.8420.7940.107
0.4120.2870.220.1670.1250.1080.6320.5510.4480.3110.2270.091
0.5450.390.2890.2170.1650.130.70.5820.4890.360.2950.446pool
0.4250.3170.2530.1920.1620.1410.8250.8040.7440.6290.5570.427
0.3080.2330.180.1440.1170.1010.2660.2320.20.1790.1560.489
0.3560.2640.2030.1630.1340.1090.4940.3660.2920.2190.1660.601
0.4950.3880.3160.2490.2050.1690.2940.2390.2040.1760.1440.743
0.5260.3960.2890.2290.1870.150.2850.2660.230.20.1770.778
plate-2HT251py-oral
oral0.7430.7480.6830.6840.6840.5970.3620.3150.2520.2070.1540.099
0.7680.7690.660.6750.6410.5980.3870.3340.3060.2410.2010.096
0.6320.5440.4610.3460.2770.1940.3290.3010.2540.2160.1870.413pool
0.6030.4370.3410.230.1730.1270.2790.230.2120.1840.1560.408
0.2250.1650.130.1040.0910.0850.3210.2510.2510.2140.1960.553
0.8250.80.7980.6980.6970.6260.2580.2180.1920.1730.1590.686
0.5680.3990.3090.20.1420.1150.2390.190.1830.1720.1510.773
0.7450.6930.7030.5990.5420.5070.2380.1930.1850.1750.1630.852
plate-3HT266pro-only
oral0.6640.6670.5970.5950.4740.3560.7950.7430.6620.6350.570.065
0.6460.5550.4180.3450.250.160.2590.2110.1580.1290.0870.052
0.5050.4120.2850.2210.1780.120.2560.2310.1830.1510.1160.438pool
0.3990.3010.2140.1710.1360.1030.2640.220.1760.1470.1080.428
0.3990.2850.2150.1620.1260.10.2130.1720.1390.1180.0980.408
0.5260.4350.3040.2320.1580.1180.2280.1730.1480.1230.1010.509
0.360.2820.2160.1610.1310.1090.3710.270.2160.170.1250.676
0.2870.2190.1710.1250.1130.090.1770.1580.1360.1190.0740.694
plate-4HT2230PY+pro
nasal0.6720.5680.4480.3280.2360.170.3540.2810.2290.1730.1290.05
0.4490.3070.2230.1560.1190.1020.6060.4920.3730.2650.1840.049
0.6470.5180.3850.2680.1850.1320.6450.5960.50.3960.2910.283
0.6080.480.3590.2470.1710.1250.4080.2850.2020.1510.1140.265
0.6660.5730.4680.3270.2220.160.7040.6590.6120.5270.4270.379
0.4970.3510.2580.1840.1360.1070.6080.4950.3810.2670.1950.519
0.6360.5090.3820.2620.1810.1320.5470.4220.3080.2270.1680.591
0.6550.5390.4270.2990.2110.1560.6540.6180.5410.4610.3480.623
plate-5HT251pro only
nasal0.7380.6830.6240.5560.4550.3370.2440.20.1630.1410.1180.079
0.6980.6220.530.4180.2960.1920.1630.1360.1140.10.0870.076
0.6430.5630.470.3480.2380.1550.1160.1020.0910.080.0740.249
0.6340.5320.4110.2820.1840.1210.1430.1190.1070.0920.0850.258
0.7310.6680.5990.5080.390.2690.1670.1340.1120.090.0840.39
0.7340.6880.6060.5080.3710.2430.1430.1240.1080.0920.0830.524
0.8060.7670.7380.7090.6730.6080.2830.1890.1440.1140.0920.576
0.7230.6140.5630.4440.3250.2230.2010.1760.1560.1230.1220.662
plate-6HT266-ip
0.1490.1190.110.1050.0990.0970.3660.3050.2320.1780.1520.078
80.2730.2020.1540.1260.1030.0870.2180.1980.1490.1240.1070.085
70.2160.1460.1180.0960.0810.0690.1760.1440.1220.1030.090.323pool
50.2770.1880.1340.1040.0850.0720.1810.1550.1280.1180.1010.365
40.2560.1680.1310.1080.0870.0750.2220.1840.1590.1310.1090.384
30.2980.2030.1480.1170.0960.0820.2030.1810.1520.1280.1090.548
20.2770.1940.150.1160.0990.0850.2520.2190.180.1430.1160.655
10.330.2070.160.1380.1220.1080.2940.2560.2060.1590.1290.71
PY79
20-SepHT251
oral0.8050.7360.7090.6210.5210.3210.4580.390.3290.2740.1960.05
0.8010.7720.7220.6190.4990.3570.2980.2730.2460.1650.1310.054
0.7340.6360.5360.3920.2630.1680.3070.2570.2010.1690.1210.416
0.6750.6080.4990.4010.2580.190.3320.2510.2310.1730.130.435
0.3980.3670.3060.2480.1860.1130.3530.2840.2240.2080.1550.246
0.8310.8060.7880.7340.6410.4990.3840.3410.2830.2050.1590.363
0.6350.5220.4090.290.1980.1350.3240.2830.2330.1780.1450.469
0.7930.7120.6180.5470.4960.3530.3610.2930.2660.2010.1410.589
IP251-ipMIX
17-80.3490.3070.240.1830.1420.1060.2960.250.2090.1590.1190.053
17-70.3650.3020.260.210.1550.1130.330.2770.2450.1970.1520.046
17-60.2640.2490.210.1660.1240.0950.2880.2260.1890.1460.1040.476
17-10.3090.2830.2530.1990.1490.1140.3850.3280.2720.2160.1580.502
16-80.2880.2610.2260.1860.1430.110.3260.2570.2340.1890.1380.263
16-70.2820.2560.2270.1880.140.1090.3630.3110.2650.2280.1590.392
16-40.3350.2920.2420.1940.1410.1070.3640.3090.2810.2380.180.558
16-10.360.310.2440.1950.1480.1080.3260.2960.2470.1960.1550.638
230-ippy-ip
ipPy+propro-only
15-80.3190.3010.2390.1860.1340.1020.3910.3370.2720.2030.1340.045
15-70.3060.2770.2410.1870.1430.1020.3520.30.2660.1940.1470.049
15-60.3310.3080.2440.1730.1270.1010.4030.3680.3140.2480.1510.485
15-50.3970.3520.2930.2020.1560.1080.4070.350.3080.2410.1650.497
15-40.3310.2870.2280.180.1280.0930.3050.2630.2320.1780.1280.204
15-30.3780.3460.2770.2050.1520.1070.3140.2540.2270.1770.130.341
15-20.4230.370.3090.2340.1710.120.4630.3610.30.2280.1690.46
15-10.420.3740.2870.2220.1550.1120.4170.3150.2780.1980.1380.585
21-SepHT230Nasaloral
0.7970.6070.390.2560.1850.120.340.1990.1430.1120.0880.043
0.3850.2150.1340.0980.0750.0590.3340.1860.1340.0960.0730.033
0.7120.4390.2680.1730.110.0750.2880.190.1140.0940.0750.134pool
0.5690.3420.2120.120.0840.0570.2460.1390.1070.0940.070.128
0.6160.4240.2490.160.1030.070.1640.0870.070.070.0450.292
0.490.2780.1720.1160.0810.0550.2530.1130.0690.0740.0560.5
0.6940.4250.2410.1580.0990.0740.3110.2110.140.1080.0810.57
0.6620.4090.2440.170.1230.0870.3380.1970.1320.1030.0870.729
IgA
HT251Nasaloral
0.9670.8870.7720.6070.4290.2710.960.9250.8830.8140.710.026
0.8660.6880.4540.280.1820.1140.930.8570.8130.7380.6270.033
0.7340.5260.3250.2110.1380.0910.6370.4460.2820.1930.1440.124pool
0.5740.2990.1740.1080.0860.060.6360.440.2740.1630.110.121
0.9220.7950.5890.3930.2450.1470.1520.0990.0740.0560.060.376
0.8990.7460.4820.2490.1530.0910.9420.9350.8540.8030.6930.531
1.051.0230.9660.9520.8880.7830.5790.3590.2040.1390.0980.653
0.860.6720.460.320.220.1360.9070.8880.8030.7010.6590.691
HT266Nasaloral
0.4990.2860.1680.1110.0750.0560.8020.7150.5780.5210.4650.044
0.7390.6170.4580.3290.2380.1390.5290.390.2460.1630.130.024
0.5780.4040.2440.1390.0850.0620.5550.2530.1510.2080.090.176pool
0.7140.5960.4190.2810.1890.1440.2710.1850.1160.0940.0640.168
0.8240.6120.3610.280.1770.0950.3430.2160.1120.0880.0610.381
*0.390.2140.1410.1010.0690.0470.3960.2610.1890.1280.0840.522*
0.2120.1490.0890.0660.0530.0390.4150.1870.1230.0850.0620.549
0.3890.2290.1280.0810.0670.0360.2850.2070.1140.090.0450.538
HT230Nasaloral
0.8060.620.4020.2640.1920.1240.3470.2030.1460.1160.0930.047
0.3880.2190.1390.1020.0770.0610.3440.1890.1350.0990.0750.035
0.7230.4540.280.1810.1150.0770.2950.1920.1130.0910.0770.142pool
0.5820.3550.2220.1260.0880.0590.2540.1420.1090.0940.0720.139
0.6270.4370.2570.1660.1070.0710.1660.0890.0710.0710.0470.308
0.4950.2860.1780.120.0830.0560.2570.1150.0710.0760.0580.526
0.7020.4370.2470.1630.1040.0770.3230.2170.1430.110.0850.611
0.6660.4170.2520.1750.1290.0920.3490.2060.1390.1080.0940.788
3-Octrepeat IgA4080160320640
9-10.8730.7470.570.3860.2570.1570.960.9220.8590.7710.6140.8432-8
9-20.5740.3730.2490.1610.1150.0890.9680.9310.8830.80.6670.635-1
9-30.7150.4970.330.210.1450.0920.2340.1750.1340.1080.0880.4235-4
9-40.8250.6710.5120.3480.2440.1610.6820.4880.3460.2410.1740.2515-5
9-50.3660.2380.1690.1220.0960.0750.2990.2090.1570.1190.0910.2826-1
11-80.8810.7880.660.5090.3470.210.4630.3190.2330.1690.1290.2546-3
12-20.050.0360.0250.020.0150.010.0940.0520.0390.0330.0270.0056-5
12-80.4870.2960.1850.1260.0820.0620.9250.8150.7470.6080.4340.0356-8
40801603206401280
Saliva-IgA
24-Sep
py-1
0.1680.130.1070.0880.080.0710.130.0870.0750.0690.0550.047
0.030.0460.0540.0580.0630.0610.0450.0520.0610.0630.0480.04
0.0730.0790.0810.0720.070.0690.0310.0460.0510.060.0470.189
10-20.0360.0480.0520.0520.0610.060.0290.0380.0440.0490.0390.264
0.0790.080.0740.0670.0680.0630.0670.0770.0740.0590.0460.381
0.0170.0250.0370.0420.0540.0540.0320.0570.0560.0520.0440.552
0.0440.0640.0630.0560.0650.0560.0710.0790.0740.0620.0480.674
10-10.0170.0240.0270.0330.0490.0520.0240.0350.0440.0360.0390.746
10-3
10-Oct
251-oralpre1st2ndfinal
10.0390.0610.0580.060.0540.0510.0530.0620.0620.0610.0630.065
20.0190.0260.0290.0320.0330.0360.0430.1040.0890.0740.0680.069
30.0380.0220.0270.3240.1880.1320.1010.9670.9010.7020.5410.367
40.0220.0180.0220.0210.0210.0250.0360.0390.0440.0460.0510.057
50.0250.0070.0250.0230.0260.030.0390.0220.0290.0290.0370.05
60.0210.1010.080.0320.0360.040.050.2380.2240.1760.1390.113
70.0260.0650.0610.0510.0520.0490.0550.470.450.3230.2660.062
80.0190.2430.1870.050.050.0530.0581.03910.8620.7150.075blank
average0.067875
251-nasalpre1st2ndblankfinal
10.0580.0440.0340.050.0540.0520.0620.3070.2120.1740.1480.119
20.0720.0430.0420.6920.530.3840.3180.8420.7840.6240.4870.311
30.0910.0230.0250.040.0330.0310.0370.8020.6850.5190.3650.272
40.0350.0610.0550.1110.0830.0720.0690.4560.3260.2250.1480.109
50.0440.0390.0370.0480.0410.0350.0390.2070.1730.1370.1010.106
60.0520.030.0270.1360.0980.0710.0570.6160.4880.4470.30.249
70.050.0450.0450.1230.0920.0740.0650.5540.4410.3130.2470.182
80.140.0650.0610.1450.1230.10.0760.6080.5190.4280.2790.21
0.04375
266-oralpre1st2ndfinal
10.0310.0220.020.0540.0510.0520.0560.0320.0370.0380.0450.052
20.0250.0270.0290.0370.0380.0420.0480.0790.0690.0630.060.062
30.0860.0610.0550.0750.070.0670.060.0670.0750.0630.0570.057
40.0260.0280.030.0650.0670.0650.0610.0520.0480.0440.0480.053
50.0390.0370.0370.0330.030.0310.0390.0380.0430.0450.0460.053
60.0240.0420.0420.0460.0470.0510.0530.0460.0480.0520.0580.062
70.0410.0540.0460.0280.0280.030.0350.0660.0570.0590.0540.059
80.0350.030.0590.030.0320.0390.0450.0740.0740.070.070.068
0.037625blank
266-nasalpre1st2ndfinal
10.2430.0750.0640.0760.0770.0710.0730.2070.1290.1110.0960.095
20.0760.0530.0540.0840.0890.0820.0820.8060.6260.5030.3680.285
30.2560.060.0550.0880.0760.0750.0720.380.2390.1790.1330.122
40.1090.0410.0430.0910.0660.060.0680.6950.6190.4420.3330.255
50.2460.0430.0420.0890.0740.0650.0680.510.3560.2730.1960.159
60.070.0390.0390.0330.0330.0340.0440.1540.1250.1150.1040.103
70.0570.0690.0530.0640.0540.0550.0580.1130.0970.0880.090.093
80.2920.0950.0610.0990.0870.0780.0770.2270.1570.1190.110.099
0.059375blank
ht230-oralpre1st2ndfinal
10.0350.0490.0490.0520.0560.0640.0550.0920.0970.1060.0970.088
20.0720.0830.0670.0560.0570.060.0590.0330.0390.0360.0410.049
30.0270.0260.0250.1260.1090.0840.0740.2390.1820.1380.1050.09
40.0930.0640.0580.0460.0420.0440.0460.0640.0610.0550.0590.059
50.0350.0510.0470.0550.0470.0510.0530.0760.0730.0710.0740.068
60.0350.030.030.0480.0470.0480.050.1350.1210.1030.0940.081
70.0450.1820.0610.0560.0620.0740.0640.150.1560.1340.1120.091
80.1030.1130.0670.0390.0450.0650.0510.0560.0530.0520.0550.059
0.07475blank
ht230-nasalpre1st2ndfinal
10.0980.140.1320.0690.0870.110.0820.080.060.0570.0640.089
20.1130.1420.1220.1160.1160.0970.0810.1090.0890.0860.0780.091
30.0940.1310.130.2120.190.1270.0970.0690.0630.0650.0640.085
40.2470.1280.1390.0550.0650.060.0540.2450.1960.1870.1610.138
50.1550.0510.0460.1180.1120.0880.0740.1050.0940.0880.0860.099
60.3340.1830.1590.030.0330.0360.040.1490.1270.1140.110.107
70.070.1950.060.0790.0710.0730.0660.0860.0850.0820.0760.094
80.1080.1590.0830.0870.0840.0930.0860.3310.30.2490.2210.188
0.141125blank
11-Oct
oralprefinalpro-onlyprepy+pro
0.0790.0690.090.0770.0670.070.1260.1080.0780.060.0740.085
0.0710.0550.0420.0380.0310.0490.0290.1090.0860.0590.0580.084
0.0760.0550.0510.0390.040.0440.0310.0620.050.0410.0510.078
0.0680.0560.0550.0870.0730.0590.0550.2920.2030.120.1040.115
0.0460.0530.0340.0280.0280.0460.0360.0450.0420.0480.0490.078
0.1110.0480.1120.0690.0580.060.0380.0520.0550.0520.0550.085
0.110.050.0310.0280.030.0340.0390.6030.4040.2890.1950.165
0.0770.0590.3220.1990.1250.1010.0490.7420.550.3620.2680.214
blank40801603204080160320
nasalprefinalpro-onlyprepy+pro
0.0610.0550.1530.1240.0950.0770.050.4950.3270.2250.1650.136
0.0580.040.0950.0770.0680.0610.0440.250.1770.1320.1070.089
0.0580.0510.0460.0440.0430.0420.0220.1580.1250.1010.0810.074
0.0570.050.1090.0950.0830.070.0730.8540.7280.5940.4810.381
0.0570.0470.0710.0640.0620.0580.0350.0690.0590.060.0540.06
0.0570.0270.0830.0680.0610.0570.0580.4940.3550.2570.1910.142
0.0550.0430.0690.0630.0580.0520.0160.9130.8290.6580.5120.351
0.0550.0440.0370.0360.0370.0410.0610.1560.1290.1220.0950.08
blank40801603204080160320
IPprefinalprefinal
266-10.0910.1170.1030.0910.0830.10.0970.0790.0750.0780.0930.102
266-20.090.0880.080.0830.0750.0950.0950.0810.0780.0720.0790.099
266-30.0610.0750.0580.050.0380.0510.0960.0530.0550.0570.0720.082
266-40.080.0840.0760.0670.0560.0560.240.1040.0910.0790.0740.082
266-50.0740.0950.0870.0780.0590.0610.0850.070.0690.0650.060.08
266-70.070.0780.0750.0670.0560.0640.1080.130.0910.0850.0680.067
266-80.0640.1010.0850.0780.0770.0920.0740.1240.1050.0860.0750.071
Blank0.0740.070.0880.0930.090.110.3480.0830.0790.0940.0960.111
IPprepro-IPprepy+problank
10.0650.0690.0630.0780.0850.0730.0490.0580.0730.0910.0840.105
20.0670.0730.0780.0760.0810.0380.0960.0810.1090.1040.0980.102
30.0870.0380.0450.0510.0570.0460.0960.0650.0860.0960.0770.09
40.1170.0330.0360.040.0480.0680.0450.0460.0650.0760.0710.094
50.001000.0010.0020.0020.002000.00100.001
60.1540.0420.0480.050.0590.0960.0940.0710.0890.0950.0820.09
70.0930.0790.070.0660.090.0910.0680.0570.0650.0960.0720.112
80.080.1160.0950.0820.0930.0760.1080.0770.0870.0880.0990.106
prefinalprefinalprefinalnaive1naive2
0.0720.1360.1060.0230.0840.0650.0960.0590.0570.0950.1210.067
0.0870.0830.0820.0460.1760.1310.0970.110.0930.040.0630.07
0.0350.0930.0890.0140.0690.0570.0350.0560.0540.0590.0590.063
0.0350.1080.1130.0570.1020.0780.0460.0610.0570.0660.060.065
0.0570.0920.0910.0280.0680.050.0260.0960.0840.0580.0590.064
0.0310.0750.0670.0420.0590.0480.0460.0780.0680.0660.0580.065
0.0460.0460.0420.0380.1080.0720.0680.0990.1090.0610.0590.063
0.0180.0740.0760.0570.1070.0730.1110.0560.0540.0580.0580.063
py-oralpy-nasalpy-ip
IgA-feces
8-Oct
230oralnasal
10.1090.0690.070.0560.0540.050.0880.0680.0610.0580.0520.761
20.0810.0660.0740.0640.0550.0510.2540.1850.1120.0830.0620.598
30.1140.0710.0660.0570.050.0450.1620.1140.0930.0710.060.447
40.1540.1080.0970.0750.0610.0540.4030.2230.1490.1010.070.298
50.1870.1480.1530.1380.0960.0850.1750.1080.090.0740.0620.045
60.1490.090.0870.0760.0610.0550.1990.1360.1060.0820.0680.049
70.2550.1930.180.1290.0890.0780.3350.2170.1740.1280.0910.17
80.2770.160.1260.0910.0710.0650.7260.5760.3810.2340.140.15pool
251nasaloralpos
10.6750.4530.3430.2290.1430.1030.1950.1270.1060.0830.0770.761
20.8180.660.5530.3780.2370.140.870.8510.8550.7560.6820.601
30.5450.2750.2190.1360.0880.0630.620.4640.3810.2580.170.431
40.440.2370.1850.1180.0710.0630.1530.0830.0760.0650.0530.303
50.5580.3470.2480.1460.0890.0690.1220.1450.1370.10.0730.052
60.5590.3430.280.1710.0990.0740.1050.0730.0710.060.0540.056
70.8680.6340.6060.4080.230.1450.5310.3330.2190.1390.0950.192pool
80.590.3740.3040.2110.1140.0920.7990.6050.4660.2640.1940.175
266oralnasalpos
10.2960.220.1530.1210.0930.0780.7250.5240.3820.270.1820.835
20.3130.2260.1580.1160.0840.0690.4860.3650.2630.1880.1410.69
30.1160.1080.0870.0740.0640.0560.7170.5720.4250.2740.1720.519
40.1530.0920.0860.0740.0650.0590.8790.7530.6360.4560.3070.377
50.4240.2180.2030.160.1240.1041.1890.9310.770.5020.3070.114
60.2190.1210.1090.0890.0690.0620.8590.7230.5790.3990.2520.056
70.2170.1280.1110.0910.0720.0630.450.3250.2430.1810.1340.13
80.5140.2920.2370.160.1110.0860.5010.3620.2540.1850.1340.167
266oralnasalpos
10.2960.220.1530.1210.0930.0780.5010.3620.2540.1850.1340.835
20.3130.2260.1580.1160.0840.0690.4860.3650.2630.1880.1410.69
30.1160.1080.0870.0740.0640.0560.7170.5720.4250.2740.1720.519
40.1530.0920.0860.0740.0650.0590.8790.7530.6360.4560.3070.377
50.4240.2180.2030.160.1240.1041.1890.9310.770.5020.3070.114
60.2190.1210.1090.0890.0690.0620.8590.7230.5790.3990.2520.056
70.2170.1280.1110.0910.0720.0630.450.3250.2430.1810.1340.13
80.5140.2920.2370.160.1110.0860.7250.5240.3820.270.1820.167
9-Oct
Py+pro-oralpro-oralpos
0.3160.2120.1470.1160.0980.0850.2670.1670.1140.0960.0760.757
0.3040.2310.1750.1330.1060.0860.1660.1240.1030.0860.0690.581
0.3050.1660.1070.0760.0620.0530.210.1870.1490.1030.0730.37
0.6180.5090.3840.2510.1860.1210.3350.2510.1880.1320.1130.231
0.4730.3430.2830.2180.1580.1090.1680.1530.1250.0890.0730.046
0.0750.0620.080.0790.0720.0690.2990.190.1270.0950.0770.055
0.4820.3750.3050.2330.1620.1410.2050.1440.1180.0910.0740.292
0.8020.6710.5470.3740.2310.160.3540.270.2110.1560.1240.308
Py+pro-nasalpro-nasalpos
0.2290.2090.1920.1480.1380.1090.4380.3190.2510.2110.1820.879
0.7130.5450.4280.2910.2180.1670.4520.2850.2380.1720.1430.723
0.3880.2840.2250.1640.1260.1140.3730.2820.2320.1880.1470.55
0.6010.4290.3130.1990.1370.10.820.6680.4830.2890.1920.377
0.640.4330.3710.2740.1970.1530.1490.2710.3510.2940.2340.083
0.5260.4110.2910.2040.1480.1350.1960.1690.1870.1660.1350.089
0.8060.7110.5380.3640.2330.1680.2460.1960.1660.1430.1120.362
0.6310.5680.4830.4110.3220.2390.2490.2190.2070.1620.1440.374
Py-oralPy-nasalpos
0.3870.3870.3640.2790.2090.1650.3720.2490.2210.1650.1340.693
0.2650.150.1540.1540.1320.1080.2540.2010.1760.1650.1240.592
0.3210.2380.2020.1730.1410.1260.4760.2960.2380.1960.1420.39
0.4450.2370.1710.1370.1210.1110.4280.2550.1940.1420.1180.288
0.2360.1290.0990.1050.1130.0850.2630.1560.1190.10.0920.07
0.3480.1970.1280.1340.1050.0910.2410.1780.1320.1160.1020.082
0.2910.1730.1140.0980.090.080.2140.1620.1280.1170.0960.312
0.160.120.1130.0980.0970.0890.4080.2320.2170.1690.1390.326
HT266-ippos
4-10.1290.0950.0960.0850.0810.0770.8210.7080.5530.4340.3230.68
4-80.260.2010.190.1170.1040.0720.5590.4350.3160.2040.140.552
5-30.7680.5730.4150.270.2020.150.4650.310.2440.1760.1750.393
5-40.2250.1710.1380.1080.0950.0880.4290.3240.2470.1850.1650.306
6-80.4780.3130.2270.1560.1080.0930.3990.2020.1350.1060.1070.065
12-70.3580.2810.2080.1340.1280.0940.1320.2370.280.2280.2070.067
nave0.3970.2480.1750.1120.0980.0890.3350.210.210.1650.1520.237
py7-10.3210.2250.170.1180.1030.090.0920.080.0710.0620.0730.257
IPPYpos
17-10.0510.0390.0390.0360.0370.0540.3090.1530.1510.1030.0810.713
17-60.1410.1170.0980.0740.0760.0990.2040.1570.140.1190.1020.607
17-70.0590.0520.0410.0390.0540.0870.4650.2530.1870.1370.1080.461
17-80.0590.0430.0340.0290.0390.0530.2150.1350.1060.0780.0650.334
16-10.5410.4330.2640.1620.1380.0930.2480.160.1380.1040.0720.037
16-40.3970.2490.1730.1150.1090.0840.3680.2260.1650.1140.0840.039
16-70.4730.420.3510.2690.2620.2090.4840.3240.2410.1760.1520.271
16-80.2540.1540.1230.1020.0890.0780.0430.0430.0420.0410.0510.238
final-IgG
25-SepTOTAL IgG
plate1HT230-ORALpy-oral
0.0730.0440.0320.0310.030.030.0810.040.0350.0290.030.034
0.0990.0420.030.0270.0260.0240.0790.0430.0350.0270.0290.028
0.3120.0940.0570.0430.0310.0280.1070.0540.0410.0280.0260.105
0.0890.0410.0340.0320.030.0250.0870.0470.0370.0250.0260.1
0.0860.0420.0320.0290.0280.0250.1140.0440.0340.0320.0240.708
0.0930.0390.030.0270.0270.0260.0960.040.0330.0290.030.808
0.0950.0390.0340.0330.0280.0290.0760.0450.0340.030.030.776
0.2690.0650.0470.0340.0290.0250.1330.050.0370.030.0340.848
plate2HT251-ORALprotein-oral
0.7250.670.6430.6330.6110.5910.6340.5840.5470.4250.2660.026
0.7120.6550.6150.5920.5550.490.0680.0480.0340.0270.0240.02
0.5370.4570.3690.2650.1730.1030.0570.0310.0260.0210.0190.069
0.1070.0450.0320.0260.0250.0220.0610.0340.0270.0240.0180.074
0.0890.0320.0250.0210.0190.0220.0620.0320.0230.0190.0160.6016400
0.7120.6660.6240.5980.5660.5520.0650.0340.0260.0190.0170.5943200
0.3740.1370.0750.0450.0320.0250.0520.0290.0210.0180.0140.5861600
0.6810.6380.6110.5770.5480.5080.0460.0240.020.0170.0150.663800
10020040080016003200
Plate3ht266-nsalht266-oral
0.3580.1810.1110.0620.0410.0330.4990.460.4070.3220.2220.025
0.5410.5090.4440.4140.3210.1860.5430.4720.3770.2610.1550.019
0.3590.160.0910.050.0340.0220.2510.0780.0420.0250.0190.069
0.6120.5630.5240.4680.4080.3430.2160.0860.0450.0330.020.077
0.5890.5260.4830.4080.3140.2230.4160.3760.3440.3260.2560.539
0.5570.450.3940.2590.1430.0990.3040.1310.0820.0460.030.533
0.4730.2440.1410.0810.0430.0260.040.0170.0130.0130.0110.442
0.5570.40.2820.1580.0980.0490.0640.0290.0230.0160.0130.514
plate4ht251-oralnasal230-nasl
0.5990.5540.5190.4730.4010.3050.4960.3230.1990.1130.0630.02
0.5080.4570.4020.2990.1980.110.3980.1710.0870.050.030.018
0.540.4810.4410.3780.2880.1910.4220.2380.140.080.0460.058
0.4830.3220.2060.1190.0740.0440.5370.4360.3260.1980.1090.059
0.5920.5350.4950.4340.3390.2320.5030.4130.3050.190.1050.525
0.5690.5080.4220.3170.1910.1110.3510.1480.0830.050.0290.566
0.6120.5530.5110.4620.3880.2780.3410.1340.0740.040.0260.567
0.5370.4150.3050.1920.1150.0640.4840.3190.1940.1110.0590.633
plate5266-ip251-ip
0.0690.030.0340.0240.0240.0180.580.4790.390.3360.2280.022
0.5860.3470.2630.1340.0860.0490.3310.1630.0940.0520.0350.023
0.0920.0350.0280.0270.020.0220.5550.4480.2450.1930.150.05
0.4810.230.1590.0860.0620.0450.4190.2480.1420.0760.0490.05
0.6010.4620.3550.2320.1390.0840.0590.0290.0210.0270.0170.569
0.3290.1040.0680.0460.0340.0250.1490.0480.030.0270.020.573
0.0990.0360.0290.0220.0210.0190.3350.1510.0820.0440.0290.604
0.4370.2130.1480.0650.0440.020.0470.0280.0220.0170.0180.662
230-ip
plate6pro-nasalpro-oral
0.0670.03-0.0140.0160.0140.0140.5980.5640.5410.4750.5090.014
0.0410.022-0.0130.0120.0170.0160.3730.2230.1420.0760.050.011
0.0460.023-0.010.0150.0150.0150.1840.0960.0440.0310.0250.076
0.0370.024-0.0080.0150.0150.0160.0180.0190.0160.0130.0150.061
0.1320.038-0.0170.020.0170.0180.4210.3990.2490.1860.1260.56
0.0350.024-0.0110.0150.0160.0160.2270.0630.0450.0280.0230.514
0.2350.084-0.0280.0280.0190.0160.0670.0180.0160.0140.0120.522
0.0580.021-0.0120.0120.0130.0120.0430.0170.0140.0120.0120.562
plate7py+pro nasalpynasal
0.2440.1310.10.0520.0430.0290.0990.0370.0330.0280.0230.028
0.350.150.0820.050.0320.020.0430.030.0140.0280.0130.016
0.620.5140.4680.3290.2070.1190.0390.0290.0270.0190.0160.059
0.3840.1770.1120.0580.0410.0290.0610.0320.0210.0160.0210.054
0.6070.4920.490.4190.310.1650.0530.030.0240.0190.0190.532
0.5350.2960.1840.1260.0580.0370.0490.0270.020.0210.0190.591
0.5060.2230.1520.0940.0410.0260.0590.0250.0190.0150.0140.597
0.6690.5690.5230.4280.3080.1940.0580.0270.0230.0160.0140.646
27-Sep37C
pool0.3130.150.0950.0650.0470.0390.030.0280.0230.0220.020.029
py-20.3160.1120.0690.0490.0370.0310.0280.0220.0210.0210.0180.023
PY-10.2680.0950.0650.0410.0370.0320.0270.0240.0220.020.0210.022
251-3-oral0.80.7720.7890.7970.7730.7260.6770.5660.4560.2780.0170.021
251-20.550.2250.1330.0870.0550.0380.030.0220.0210.0190.0160.02
251-10.820.8390.8140.7670.7590.7250.6390.5220.3710.2270.0190.022
ip-new0.8340.8310.8140.7620.7550.6920.6060.4560.320.1860.0220.019
ip-old0.8130.7840.7220.6930.640.5640.4280.2750.1690.1010.0180.018
20004000800016000320006400012800026400
4C
pool0.2320.1460.0920.0640.0450.0350.0280.0270.0240.021-0.015-0.01
py-20.220.1140.0730.0490.0330.030.0230.0230.020.019-0.009-0.008
PY-10.1920.0950.060.0410.0310.0280.0230.0210.020.023-0.01-0.009
251-3-oral0.7240.6790.6710.620.5780.5710.5460.4760.4160.309-0.011-0.009
251-20.5310.2750.1580.0890.0490.0340.0220.020.0170.015-0.011-0.009
251-10.7410.720.7240.6850.6820.6670.6430.5330.4070.265-0.01-0.008
ip-new0.7890.8040.7820.6770.6510.6110.560.4550.2830.183-0.011-0.01
ip-old0.7080.710.6310.5890.5270.480.3780.2650.1560.099-0.012-0.01
20004000800016000320006400012800026400
dilutionbuffer:BSA0.2%
no tween
28-Sep1hour
pool0.3560.1290.0690.0350.0250.0170.0140.0120.0120.010.0090.011
ip1.0851.050.9890.9270.8110.6690.4970.3020.1640.0870.0480.01
251-3-O1.0811.0741.0110.9880.8940.7390.6010.4270.2370.140.0720.011
PY-1-N0.2380.0870.0560.0340.0210.0180.0150.0130.0070.0080.0080.009
PY-N-F0.1780.090.0570.0420.0330.030.0270.0220.0210.020.0220.028
Lung-12-40.4940.3530.2250.1370.0870.0580.0410.030.0280.0260.0240.017
251-3-O-F0.890.7820.6540.4690.280.1560.0910.0560.0380.030.0280.025
251-3-N-F0.3720.2220.1520.1080.070.0490.0380.0310.0340.0290.030.03
2hour
pool0.260.1180.0620.0310.0210.0170.0160.0160.0160.0170.0110.008
ip0.8540.810.7930.7370.6690.5890.4720.3260.20.110.0610.007
251-3-O0.8820.8670.8370.7980.7160.690.5880.4210.3050.1670.0920.007
PY-1-N0.2240.1030.0740.0420.0230.0170.0120.0080.0080.0070.0060.008
PY-N-F0.2450.1120.0730.0470.0380.030.0280.0210.0180.0180.020.02
Lung-12-40.4970.4120.2830.1810.1010.0640.0450.0340.0240.0210.0190.02
251-3-O-F0.7680.7140.7150.490.3110.190.1150.0660.0420.0290.0260.02
251-3-N-F0.340.2340.1820.1130.0770.0560.0440.0230.0270.0250.0230.023
1-Oct
pool0.0390.0460.040.0420.0460.040.0380.050.0340.0290.0360.06
Nave0.1710.1230.10.0770.0720.0550.0430.0460.0380.0460.0430.065
PY-20.0410.0470.0430.0410.0380.0390.040.0420.0450.0390.0410.039
PY-10.1620.130.1040.0790.0620.0520.0430.050.0450.0470.0360.042overnight incubation 1st ab
5-30.5830.5810.5940.6080.6580.6960.7210.530.2990.1830.0750.046
5-20.3550.2630.1980.1230.090.080.0560.0510.0470.0480.050.057
5-10.6070.570.5780.6450.6690.6530.5340.360.1810.1250.0760.052
IP0.4440.4450.460.4480.4820.4930.5990.4260.2210.1370.0890.055
1-Oct
251-oralIP
10.8210.8120.8120.7410.7330.6580.6650.6770.5040.3370.8010.007
20.5120.2720.1720.10.0580.040.0230.0160.010.0090.7150.011
30.8660.8530.8940.8370.8340.8140.7580.6260.5520.40.7820.006
40.1230.0440.0220.0140.0120.0140.010.0090.0050.0040.6630.006
50.2090.0760.0470.0280.020.0160.0110.0060.0050.0040.5750.005
60.6820.6590.4640.380.2880.1470.080.0460.0240.0130.4370.008
70.7930.7910.80.7450.7120.6190.5670.4780.3350.1710.2760.11pool-py
80.8350.7930.8290.7770.7350.670.6860.6740.4930.4010.1590.121
5010020040080016003200
251-nasalIP
10.7220.6650.5650.4060.2470.1390.0730.0370.0190.0140.890.017
20.7760.7970.7720.730.6860.5780.450.2750.1380.070.8550.007
30.7390.7370.6960.5960.4190.2710.1420.0730.0340.0180.8030.005
40.7690.7780.7490.7020.5830.4860.330.1670.0770.0450.7380.008
50.7270.5850.430.2810.1460.0860.0470.0230.0110.0050.6170.006
60.80.7240.6940.6520.5090.3980.2550.1360.0630.0340.4490.009
70.7870.7290.6230.5390.370.1980.1080.0560.0240.0150.2760.117
80.8480.8110.8090.7420.670.550.4080.2560.130.0760.1680.123pool-py
5010020040080016003200
266-oral5010020040080016003200IP
10.0810.0360.0260.020.0140.010.0080.0090.0070.0080.6090.011
20.050.0180.0120.0090.0060.0040.0030.0050.0060.0040.6020.005
30.3410.1850.1070.060.0310.0160.0140.0060.0050.0030.5840.005
40.540.5050.4490.420.3540.260.1730.0950.0550.0250.5490.005
50.3780.1650.0980.0540.030.0190.0140.0060.0030.0040.4910.004
60.2670.1210.0680.0360.0210.0110.0080.0060.0040.0040.3580.005
70.7250.6820.6330.5760.4980.2960.1930.0890.0420.0220.2120.09
80.7330.6790.620.570.4680.3150.1850.0970.050.0220.1350.094pool-py
5010020040080016003200
266-nasalIP
10.4730.3560.2780.1720.1020.0610.0380.0170.0080.0050.4520.002
20.4390.3320.2120.1220.0820.0410.0230.0120.0060.0060.5590.001
30.50.5050.5110.4060.2770.2310.1210.0510.0230.0120.5190.003
40.630.5910.5470.4960.4490.3520.2610.1130.0570.0210.4850.003
50.6250.5750.5190.510.4960.3810.270.160.080.0450.4090.002
60.5120.3110.1890.1130.0530.0330.0170.0080.0070.0050.3170.004
70.6320.560.5160.4250.3770.2830.2030.080.0430.0220.1820.092pool-py
80.480.270.1430.0850.0470.0210.0150.0070.0040.0030.1170.086
5010020040080016003200
2-Oct
251-oralIP
11.0331.0581.0031.0121.0220.880.8020.6320.4130.2441.0890.009
20.6040.3520.2030.1120.0640.0370.0250.0190.0140.0131.0240.006
31.0661.0621.0861.0430.9820.9460.8820.7510.5310.3840.9930.008
40.1220.0560.030.0210.0180.0150.0140.0130.0090.0090.9020.008
50.2380.1080.0630.0340.0210.0150.0140.0110.0110.0080.8220.005
60.7620.7230.5780.3960.2560.1360.0780.0430.0270.0170.6560.11
70.9931.0411.0251.0090.9520.8740.7310.5480.3090.1650.4620.103pool-py
80.9871.0731.0281.0161.010.8960.8180.6610.4830.3250.2510.1030.3
20040080016003200640012400
251-nasalIP
10.8670.80.6910.4960.3010.1680.090.050.0320.0541.0990.031
21.0150.9880.9860.9110.8060.6830.4570.2790.1450.091.0480.023
30.9870.9350.90.7670.5660.3670.1980.110.0570.0371.0060.018
40.9490.9380.8370.8390.7020.5170.3120.1680.0920.0510.9190.018
50.7890.7270.5760.380.2380.1150.0650.0370.0210.0150.7770.014
60.8540.8830.8530.7730.6750.4330.2720.1480.0720.0440.6570.106pool-py
70.8540.8340.7380.5820.3850.2260.1190.0660.0350.0220.4410.103
80.8980.9920.9460.8810.8650.6370.480.2770.1450.090.2430.12
20040080016003200640012400
266-oral5010020040080016003200IP
10.0960.0570.0350.0280.0260.0210.0190.0180.0160.0150.9570.017
20.090.0490.0320.0250.0230.020.0190.0170.0150.0160.8980.016
30.7180.5690.3660.2330.1390.0730.0480.0340.0290.0230.8620.019
40.840.7950.730.6390.5120.3030.1810.0970.0560.0360.7630.019
50.4210.2540.1440.0810.0470.0290.0220.0170.0130.0110.7510.016
60.4370.2490.1180.0650.0440.0250.0150.0150.0130.0110.520.078pool-py
70.8390.8620.7230.50.360.1730.0930.0530.0270.0210.4070.096
80.8560.860.7440.6180.4920.2590.1550.0790.0410.0270.2160.086
20040080016003200640012400
266-nasalIP
10.8070.7330.5250.3250.210.1050.0630.040.0280.0271.0070.017
20.6970.5440.3470.2190.1290.0680.0440.0410.0220.0191.0470.017
30.7470.6650.5140.3380.2240.1150.0680.0410.0280.0260.9550.02
40.9520.9540.8390.8550.7390.5030.3190.1790.1050.0590.940.015
50.9791.0450.9020.9250.870.6790.4930.3080.1660.0930.7130.01
60.4820.3230.1820.0980.0610.0330.0230.0170.0130.0120.5920.094
70.9190.9060.8820.7930.6740.5130.3140.1920.0950.0510.3660.0920.2
80.3660.2310.1240.0710.0410.0270.0170.0120.010.0090.2080.096pool-py
20040080016003200640012400
3-Oct
230oralIP
10.5340.3030.160.0870.0490.030.0210.0160.0140.0131.130.014
20.1620.10.0590.0340.0220.0150.0140.0130.0120.011.0730.009
30.150.0810.0430.0250.0190.0150.0140.0130.0140.0091.0310.01
40.1870.1360.1110.1060.0860.0760.0670.0620.0560.0420.9540.009
50.110.0590.0340.0230.0160.0140.0130.0120.0120.0080.8730.011
60.6190.3450.1740.1020.0590.0310.0230.0170.0150.0120.6870.012
70.140.0770.0440.0260.0190.0120.0120.010.010.0090.4680.094pool-py
80.1870.1320.0980.0680.0430.0280.0220.0170.0130.0120.2830.0940.2
20040080016003200640012400
230nasalIP
10.9650.8030.6260.4520.2770.1490.0850.050.0310.0221.2090.01
20.8130.5940.3640.2120.1180.0650.0380.0270.0190.0151.1670.01
30.7490.4930.2970.170.0910.060.0340.0230.0190.0141.1120.012
41.0420.9470.8380.7330.5410.3180.1850.1080.060.0351.0440.01
51.0460.9320.8140.6950.4880.2910.1610.0930.0570.0330.9180.011
60.9230.7890.5960.4060.2390.1260.0710.0430.0290.0210.7180.012
70.8950.6050.3870.2090.120.0620.040.0250.0190.0150.4750.094pool-py
80.9620.8360.7250.5390.3240.1840.1060.060.0380.0260.2780.1010.2
20040080016003200640012400
251-266 ipIP
17-11.0730.9490.7480.4770.2840.1530.0850.0530.0330.0231.2690.013
17-61.1781.0870.9910.8940.7180.4620.2640.1560.0910.0531.2270.012
17-70.9980.8130.520.340.1890.1040.0630.040.0250.0181.170.012
17-81.2451.1621.0821.0750.9870.8310.7280.5190.3350.1981.1130.012
18-10.9490.7560.4940.3240.1860.1010.060.0360.0250.0170.9970.013
18-20.2210.1270.0750.0440.0270.020.0160.0170.0140.0150.7840.013
18-30.8190.5650.3280.1850.0960.0550.0340.0240.0160.0150.5580.0790.2
18-41.1421.0510.8920.7610.5260.2970.1920.1110.0640.0410.3350.074pool-py
20040080016003200640012400
4-OctHT251-ORALIP
11.0651.0461.0280.9980.9260.8950.8110.6710.4910.2951.1070.031
20.6240.360.2030.1550.0650.0430.0290.0220.020.021.0460.021
31.0741.0511.0491.0180.9310.9160.8680.70.5550.3550.9790.021
40.1260.060.0370.0260.020.0180.0160.0140.0140.0170.890.018
50.2090.0980.0580.0360.0290.0190.0180.0390.0150.0150.7380.017
60.8870.7770.7390.5390.3420.1970.1120.060.0380.0290.5590.018
71.0711.0761.0480.9890.9060.8660.7690.5560.3660.2120.3390.093pool-py
81.0681.0641.0711.020.9970.9360.8860.7690.5930.4220.1920.1010.2
HT251-NASALIP
10.9030.7730.6850.4890.2810.1580.0860.0520.0310.0241.0670.03
20.9410.8630.9130.8050.6350.4750.2950.150.090.0551.0050.012
30.9310.8860.8320.6860.4660.2850.1680.0880.0560.0330.9610.013
40.9710.9120.9080.7940.6350.490.3260.1540.0980.0550.8930.012
50.8280.6630.520.3070.1680.0970.0520.0280.0250.0160.7670.01
60.9290.8660.9050.7670.6380.5190.3280.1640.10.0540.6330.012
70.8920.7950.7780.5780.3540.2110.1210.060.0380.0230.4440.069pool-py
81.0340.9620.9770.9340.8220.7350.6050.3670.230.1270.2660.0810.2
HT266-ORALIP
10.0740.0340.0250.0170.0140.0140.0110.0110.0090.0130.8260.024
20.0640.030.0220.0160.0120.0110.0120.010.0110.0190.910.016
30.6610.4290.3430.1750.080.060.0320.0180.0160.0150.8080.017
40.7570.7080.7210.6220.3860.2440.150.070.0470.0350.7340.014
50.4390.2220.1330.0710.0410.0230.0190.0130.0130.0120.60.014
60.4090.190.1230.0610.0320.0260.0180.0130.0130.0130.4480.015
70.8450.6870.6870.4610.2430.1270.0880.040.0250.0190.2650.077pool-py
80.9040.8110.8320.6460.4620.2940.1750.0850.0540.0270.1590.0780.2
HT266-NASALIP
10.9020.6630.6230.3840.2030.1290.0680.0360.0260.0250.9390.041
20.810.5760.440.2560.1260.0830.0420.0240.0190.0180.9150.018
30.7830.6750.6760.4450.210.140.0830.0380.0290.0220.8860.014
40.9280.860.9590.870.6520.5870.4130.2070.1130.0710.8020.014
51.0340.9620.9340.8440.7480.6280.4760.2650.1610.090.7890.013
60.6470.360.2370.1280.0590.0410.0270.0160.0160.0120.6020.014
70.930.8370.9240.8380.6310.5780.3670.1720.1090.0640.4160.069pool-py
80.4870.2660.160.0820.0430.0320.020.0140.0130.0120.2620.0790.2
IPIP
HT2300.1620.10.0740.050.0320.0240.0180.0170.0170.0181.0410.012
0.6740.4950.3580.2130.110.070.0420.0240.0210.0180.9910.013
0.3120.20.1340.0750.0420.030.020.0150.0170.0130.9810.014
0.1780.1120.0780.0560.0330.0210.0180.0180.0160.0150.8820.013
HT266 50.7790.5730.4250.2530.1360.080.0490.0280.0230.0190.8070.016
70.3710.2190.1420.0880.0470.030.0230.0170.0170.0160.6220.013
80.2040.1240.0870.0570.0340.0250.020.0150.0170.0170.4630.085pool-py
PY1-IP0.20.1630.1090.0750.0480.0330.0260.020.0190.0170.2830.0880.2
6-Oct
Py-oralpy-nasalIP
10.180.0940.0490.0310.0210.0170.1180.0610.0320.0240.8780.012
20.1150.0590.030.020.0130.010.1560.0820.0380.0220.8630.011
30.0830.0380.0210.0150.010.010.1240.060.030.0180.7890.01
40.1260.0680.0360.0220.0150.0110.1230.0660.0320.020.7440.01
50.1150.0570.0280.0190.0120.010.1120.050.0230.0160.6280.009
60.1490.080.0370.0260.0150.0120.1130.0550.0270.0160.4540.011
70.0890.0470.0220.0160.0110.0090.0630.030.0170.0130.2490.065pool-py
80.0860.0450.0230.0170.0120.0110.0920.0440.0190.0130.1350.0710.2
py+pro-oralpro-oralIP
10.1280.0710.0330.0240.0180.0140.10.0620.0320.0220.880.016
20.1430.0780.0330.0220.0150.0110.1350.0690.0490.0260.8620.013
30.3960.2260.110.0620.0330.0220.1730.0970.0410.0230.7550.012
40.1350.0710.0360.0220.0170.0110.1510.0850.0480.0230.6910.011
50.7080.7520.5950.4840.2810.1630.1380.0790.0360.0190.6150.009
60.4140.2430.120.0770.0380.0250.1180.0720.0330.0220.4730.012
70.6890.6620.4110.3250.1610.0940.1930.1150.0560.0290.2510.07pool-py
80.730.8360.8030.8080.7810.7080.8140.7810.7180.5550.1460.0830.2
py+pro-nasalpro-nasalIP
10.8470.7610.7060.7430.520.3220.1670.10.0440.0210.8440.011
20.6740.5140.3220.1860.0930.0390.3980.2230.1160.0570.850.009
30.670.5270.310.2060.0980.0470.1160.0640.0280.0170.720.011
40.7290.6830.6460.6390.4820.2410.1750.0760.0370.0270.6380.013
50.5430.3820.2660.1670.0920.0350.1050.0490.0210.0170.6330.01
60.7640.7460.6350.5810.4080.2050.1130.0550.0310.0190.460.012
70.4960.3330.1850.1190.0560.0250.1260.0690.030.0140.30.077pool-py
80.5540.4560.2660.1920.0910.0390.2330.1230.060.0320.1890.0760.2
Py+pro-IPPro-IPIP
0.3660.2070.1120.0610.0360.0260.1590.0850.0470.0250.8330.016
0.2220.1070.0560.0330.020.0150.1620.0930.050.0240.790.012
0.2780.1330.0550.0380.020.0150.150.0770.0390.020.7540.011
0.2550.1460.0580.0380.020.0140.1520.080.040.0190.6690.012
0.1490.0810.0330.0260.0150.0150.1130.0610.0340.0170.5390.01
0.2110.1180.0620.0380.0220.0150.010.10.0580.0270.4150.012
0.3060.1370.0690.0410.0210.0160.0990.0570.0340.0180.230.071pool-py
0.250.1370.0670.040.0240.0180.10.0610.0260.020.1410.0790.2
Py-ipIP
naive10.070.0380.0220.0170.0140.0130.3820.2640.190.0880.9310.012
naive20.0820.0410.0220.0170.0140.0120.2020.0870.0420.0250.860.011
2-3-10.1110.0540.0310.0230.0160.0150.2030.1020.0440.0260.8210.011
2-3-80.7650.7110.6630.5680.4320.2580.2270.090.0360.0220.7390.01
0.0110.0080.0080.010.0090.010.2830.1610.0660.0330.5380.009
0.0120.0080.0080.0120.0120.0120.190.0940.0420.0240.390.011
0.0140.0070.0070.0090.0090.0090.2290.1310.0560.0280.2320.064pool-py
0.010.0060.0060.010.0090.010.3310.20.0950.0450.1410.0620.2
12-Octht266
IP18-118-218-318-418-518-718-83-53-89-15-45-5
0.5180.1980.7250.9240.8190.4040.3570.8531.1131.0090.1340.155
0.3160.1050.4820.8810.6350.2540.2370.81.1280.950.0660.09
0.150.0650.2620.6960.4520.150.1310.6071.0850.8090.0430.062
0.0990.0350.1230.5450.2260.0790.070.3761.0210.6060.0330.043
0.0570.0250.0690.3150.1190.0450.040.210.9520.4490.0270.038
0.0460.0250.0450.1710.0760.0350.0320.1150.8170.2720.0270.038
0.030.0210.030.0880.0470.0260.0270.0650.6160.1430.0260.035
pos0.9360.8830.840.8480.6990.4410.2370.1280.0360.030.3430.326pool
ht251
ht23016-116-416-716-817-117-617-817-76-16-3
0.1120.5970.4150.2030.8891.0051.0870.8040.0740.0760.0250.029
0.0920.420.2170.1330.7960.9720.960.5860.0410.0390.0240.028
0.050.2170.110.0690.5990.8410.9570.3670.030.0290.0240.027
0.0290.120.0560.0360.3770.7260.810.2170.0260.0240.0220.024
0.0220.0610.0380.0280.2240.5390.6640.1310.0240.0210.020.024
0.0230.0430.030.0250.1130.3420.440.0760.0250.0190.0220.026
0.0180.0280.0240.0240.0620.1820.3070.0480.0230.0230.0220.024
pos0.9270.8960.9760.8980.6510.4990.3060.1550.0250.0240.2330.211pool
seroIgG-alpha
18-Octoral
4-14-24-3
400.3920.3640.4730.6950.2820.3210.5170.2370.380.3430.5320.302
800.2910.240.370.6020.2010.2270.2750.190.2790.240.420.19
1600.1730.1540.2550.4170.1270.1630.2560.1290.1830.150.2950.106
3200.0950.0920.1540.2460.0770.1030.1620.0850.1060.0960.1810.063
6400.0540.0570.0960.120.0480.070.1170.0470.0670.0590.1170.038
12800.0360.0380.060.0660.0350.0460.0320.0390.0490.0460.0740.028
25600.0250.0280.0420.0490.0270.0370.0550.0330.0330.0330.050.022
IP0.8430.8370.7960.7090.6040.3880.2590.1470.0170.0180.2280.224
8001600320064001280025600blankpool
4-44-54-6
400.3470.3490.5630.2350.3790.3640.5970.1850.5060.3610.6250.8
800.2850.2530.5280.1990.3540.2680.5520.1390.4060.2420.5330.71
1600.1710.150.3810.1310.1960.160.3680.080.2670.1350.3680.511
3200.1020.1040.3070.1010.130.0950.2530.0510.1580.0850.2450.28
6400.0640.0590.1810.0560.0690.0630.1540.0270.0910.0540.1470.138
12800.0420.040.1150.0360.0470.040.0950.0210.0590.0360.0850.08
25600.030.0280.0650.0260.030.0410.0580.0170.0380.0250.0560.048
IP0.8560.8210.7330.6710.60.3950.2320.1240.0110.0110.1950.203
8001600320064001280025600blankpool
4-74-81-1
400.3570.4740.6410.2860.5250.4550.6770.230.3620.4830.6110.254
800.2520.3410.5510.2240.3950.3450.5820.1620.2210.3360.490.152
1600.1370.1950.4020.1430.2630.1920.4540.0950.1240.1990.3180.094
3200.0840.1140.2650.0950.1580.1220.3260.0570.0780.1140.1960.05
6400.0510.0740.1820.0540.0890.0760.2120.0290.0490.0670.120.033
12800.0350.0440.1020.0390.0560.0480.1310.0220.0350.0450.0740.026
25600.0240.0290.0610.0250.0350.0320.0740.0160.0250.0290.0460.019
IP0.8310.8040.7910.6890.6270.3960.2830.1670.0120.0120.1960.2
8001600320064001280025600blankpool
5-15-25-3
400.4450.4080.8090.8710.4480.5290.6050.780.4090.4740.8480.976
800.3030.2320.7070.8620.310.3620.4580.6540.2560.3030.7490.953
1600.1740.1480.5890.8380.2030.2340.3270.4810.1570.1890.6260.957
3200.1040.0930.4070.7720.1210.1510.2120.2270.0950.1110.4410.903
6400.0650.0610.270.8320.0810.0960.1550.1610.0660.067.0.2760.885
12800.0470.0440.150.7840.0510.0630.0950.0970.0420.0440.1660.876
25600.0270.030.0870.7140.0320.0380.0570.0560.0270.0290.0840.829
IP0.8960.8290.8040.6620.5820.5820.230.1260.0150.010.1840.179
8001600320064001280025600blankpool
5-45-55-6
400.2680.3010.4560.2070.2560.3540.5230.9410.3430.3870.4950.942
800.2080.1930.3320.1120.170.2240.3760.8520.2260.2470.3750.849
1600.1030.1130.2160.0550.0970.1220.2490.8350.1380.1430.2450.767
3200.0780.0650.1360.0280.0620.0770.1630.7280.0780.0870.1550.669
6400.050.0450.0820.0220.0410.0510.0990.6370.050.0510.0910.531
12800.0360.0320.0540.0170.0280.0340.0590.4920.0350.0340.0580.36
25600.0260.0220.0350.0130.020.0220.0380.3370.0250.0220.0380.218
IP0.8860.7620.7950.6470.570.360.2150.1270.0150.0130.2050.209
8001600320064001280025600blankpool
5-75-81-2py-2251-8251-7
400.2290.260.7460.8910.2610.3390.6870.9170.2760.4550.4550.161400.2180.2220.0160.0160.1550.2550.4040.6160.6370.7870.8370.816
800.1340.1590.6320.8060.1750.2240.5510.9450.1640.3010.3310.097800.0160.0360.030.0250.8670.0650.0250.0220.7430.0670.020.021
1600.1060.1130.4790.8340.110.1410.3970.9140.0970.1780.2120.0571600.020.0540.0460.030.8730.110.0380.0310.7390.1170.0320.03
3200.0630.0630.3020.7790.0650.0810.2390.8810.0620.1020.1340.0353200.0250.0850.0660.0420.8760.1590.0530.2650.7810.1860.050.037
6400.0370.050.1860.7810.2650.0530.1590.8760.0420.0660.0850.0256400.0350.1340.1020.0620.8810.2390.0810.0650.7790.3020.0630.063
12800.030.0320.1170.7390.0310.0380.110.8730.030.0460.0540.0212800.0570.2120.1780.0970.9140.3970.1410.110.8340.4790.1130.106
25600.0210.020.0670.7430.0220.0250.0650.8670.0250.030.0360.01625600.0970.3310.3010.1640.9450.5510.2240.1750.8060.6320.1590.134
IP0.8160.8370.7870.6370.6160.4040.2550.1550.0160.0160.2220.218IP0.1610.4550.4550.2760.9170.6870.3390.2610.8910.7460.260.229
8001600320064001280025600blankpoolnguoc
19-Oct
oral6-16-26-3
400.3370.5430.6540.1310.3350.6220.5050.1330.4390.5240.8141.008
800.2150.3670.4540.0750.2140.4440.3640.0880.2820.3390.6310.832
1600.1210.2240.3350.0510.1330.2890.2520.0510.1960.2130.4590.63
3200.0680.1360.2150.0320.0870.1810.1680.030.1040.130.3110.448
6400.0460.0850.1350.0210.0530.1130.1040.0220.0670.0820.1990.29
12800.030.0550.0850.0130.0360.0710.0670.0180.0480.0550.1270.163
25600.0220.0320.0520.0120.0240.0470.0430.0150.0330.0370.080.091
IP1.1281.0590.990.8310.630.380.2250.130.0150.0130.2080.201
8000160003200064000128000256000blankpool
6-46-56-6
400.2170.2660.7540.9570.2010.3390.4820.7680.2510.3170.5440.912
800.1430.1870.5540.8670.1320.2230.3330.5760.1690.2150.380.669
1600.0920.1180.3750.7430.0870.1460.2210.3640.1080.1460.2530.38
3200.0670.080.2560.6610.0640.1010.1580.2030.0720.1020.1820.189
6400.0470.0540.1620.5510.0440.0650.1070.1130.0540.0780.1270.106
12800.0410.0420.1040.4380.0320.0450.0750.0660.0390.0480.0880.066
25600.0240.0260.0640.3010.0220.0290.0520.0390.0270.0330.0570.071
IP1.0181.0140.9340.7850.6310.4160.2410.1340.0120.0110.1190.101
8000160003200064000128000256000blankpool
6-76-84-8serum
400.2940.6250.9521.1770.2510.4830.5391.1950.070.1970.2670.151
800.1450.3670.7421.10.1310.2840.3411.0920.0440.0820.1260.086
1600.0960.2290.5471.020.0820.1810.2191.0480.0320.0480.070.055
3200.0590.1460.3560.8890.0560.1230.1431.0430.0240.0290.0410.036
6400.0410.0920.2380.6640.040.0880.0950.9490.0210.0220.0260.027
12800.0320.0630.1510.4310.0280.0560.0620.7820.0170.020.0220.026
25600.0220.0410.0930.2490.0220.0350.0420.5610.0160.0180.0180.02
IP1.1361.1071.0320.9280.7370.5020.3120.1710.0150.0150.2010.214
8000160003200064000128000256000blankpool
20-Oct
1-41-51-6
400.2590.2490.3480.0890.2570.2620.3690.0680.240.4570.4070.111
800.1690.1520.2220.0490.1550.1510.2330.0390.1370.2740.2370.066
1600.0930.0830.1330.0360.0830.0850.1460.0310.0740.1550.1470.048
3200.050.0470.0830.0220.0490.0560.0920.0210.0450.0890.0930.027
6400.030.0290.0490.0170.0340.0350.0580.0170.030.0510.0550.019
12800.0250.0230.0350.0140.0260.0240.0390.0150.0260.0370.0390.021
25600.0160.0160.0210.0110.0160.0180.0250.0130.0170.0250.0260.015
IP0.6350.6230.5710.5030.3750.2330.1570.090.0120.0120.0940.087
8000160003200064000128000256000blankpool
3-13-23-3
400.4710.6030.6970.2350.4550.6910.7760.1680.5190.7990.8140.976
800.3830.4560.5810.120.3550.5360.7050.1080.4620.7240.7580.833
1600.2340.2680.4210.0740.2160.3290.4850.0720.3020.5170.5950.612
3200.1240.1460.2470.0410.1120.1880.3150.0480.1690.2910.3910.35
6400.0730.0910.1530.0280.0680.1210.2030.0330.1030.1770.2460.163
12800.050.0620.0790.0260.0510.0750.130.0310.0720.1090.1460.095
25600.0370.0410.0610.0240.0350.050.0830.0270.0510.0680.0850.056
IP0.8820.9020.80.7410.5870.390.2490.1510.0220.0210.2040.216
8000160003200064000128000256000blankpool
3-43-53-6
400.4940.6390.7020.180.5470.6750.8251.0670.510.5910.9050.954
800.3630.4440.5780.1050.4170.4030.7020.9660.3840.3910.7730.717
1600.2250.2580.3480.070.2710.3030.5120.8710.2560.2120.6050.469
3200.1210.1450.2610.0450.1430.1730.3320.7770.1410.1220.3870.256
6400.0690.090.1420.0290.0840.1010.1920.6370.0850.0730.2350.13
12800.0480.0620.0850.0260.0560.0650.1210.4720.0580.0530.1460.087
25600.0330.040.0610.0210.0380.0450.0760.3130.0410.0390.0890.056
IP0.9430.8730.7290.6890.5570.3780.2520.1420.020.0190.1270.308
8000160003200064000128000256000blankpool
3-73-81-3
400.3660.4910.5890.8070.360.5190.6990.8970.3740.440.5970.077
800.2750.3270.4510.7790.2550.3540.5780.870.2480.2970.4410.052
1600.1610.1870.2990.7070.1420.2120.4170.8860.1430.1720.2940.037
3200.0860.1130.1830.5990.0870.1240.2710.8890.0890.1060.1880.028
6400.0490.070.10.4530.0530.0730.1620.8420.0580.0670.1190.024
12800.0330.0490.0690.2950.0390.0530.1070.8090.0410.0460.0830.028
25600.0250.0360.0460.1750.0280.0350.0640.7760.030.0340.0550.022
IP0.7380.7870.6420.6450.5160.3590.2310.1370.0170.0170.1220.118
8000160003200064000128000256000blankpool
22-Oct9-19-29-3
400.4020.6140.8030.9320.4870.5890.7130.9470.5340.6620.7811.02
800.2360.4080.690.8910.2810.4530.550.8950.3560.4510.6320.956
1600.1290.230.5020.8950.1390.2830.3480.8450.2050.2740.4240.896
3200.0690.1260.30.8430.0750.1480.2020.7460.1110.1480.2610.758
6400.0420.0780.1790.7870.050.0870.1230.5750.0690.0870.1520.567
12800.030.0460.1040.7040.0360.0580.0770.3940.0510.0580.0980.349
25600.0210.0340.0610.5660.0270.0350.0470.2330.0340.0380.060.195
IP0.8870.880.8050.7260.5940.420.2680.1520.0180.0170.2210.193
8000160003200064000128000256000blankpool
9-49-59-6
400.4280.4860.6720.9150.4550.4470.70.8230.4970.6030.7231.004
800.2850.3370.5280.8590.2910.2790.5160.5880.3440.4060.5140.986
1600.1480.1890.3540.7740.1540.1530.3520.4150.2030.2320.3070.94
3200.0780.1030.2080.6920.0790.0840.2040.2710.1090.1250.1810.846
6400.0490.0610.1290.5790.050.050.1260.1560.0630.0680.1040.738
12800.0360.0450.0890.4840.0340.0350.0790.0970.0420.0440.0680.603
25600.0230.0270.0510.3150.0240.0220.0480.0610.030.0320.0420.424
IP0.8980.860.7970.7380.6450.4690.3180.1910.0170.0180.210.203
8000160003200064000128000256000blankpool
9-79-810-1
400.7250.7650.8430.8030.5130.5960.8290.9550.5150.6940.7681.041
800.6320.6530.7690.6870.3580.3970.6690.8620.2610.4070.5320.981
1600.3890.3860.5480.5290.1870.1940.3840.6670.1180.1930.310.898
3200.2080.2150.3360.3260.0970.0990.2170.3860.0670.1080.1760.69
6400.1130.1250.1890.1860.0570.0590.1270.2310.0430.0660.1050.448
12800.0710.0730.1120.1020.0380.0420.0770.1380.0330.0420.0660.258
25600.0450.0470.0660.0580.0270.0280.0490.0790.0240.030.0420.168
IP0.9070.8880.8160.7260.5820.3920.2520.1480.0180.0180.1760.218
8000160003200064000128000256000blankpool
10-210-310-4
400.360.5340.5790.8790.3530.4320.5240.8720.4420.3970.581.06
800.2190.3590.4260.830.1990.2590.3690.7440.270.220.4011.024
1600.1340.2250.30.7380.1150.1540.2570.5610.1610.1350.2690.856
3200.0910.1480.2020.5550.0730.1020.1740.3580.0980.0870.1830.91
6400.0640.0990.1360.3360.0520.070.1210.2030.0720.0650.1240.782
12800.0460.0630.0880.1850.040.050.080.120.050.0440.0840.631
25600.0320.0450.0610.1070.0290.0370.0570.0720.0390.0330.0560.415
IP0.9550.9460.860.7340.60.3930.2360.1350.0190.0190.210.247
8000160003200064000128000256000blankpool
10-510-610-7
400.4690.3270.5451.020.350.4030.5661.0090.3890.4750.5940.948
800.3320.2170.4320.9950.2040.2610.4190.9550.240.3250.4380.922
1600.1830.1270.3020.9390.1140.1550.2820.8710.1420.20.2940.763
3200.1170.0830.1860.8740.0710.0970.1810.7390.0880.1180.190.683
6400.0720.0530.1190.780.0480.0610.120.5110.0580.0760.1170.356
12800.0520.0390.0850.6290.0380.0460.0830.320.0440.0540.0820.252
25600.0350.0290.0520.4410.0280.0290.0510.1740.0390.0420.0550.137
IP0.9320.7490.8380.7270.5930.3960.2940.1840.0160.0160.2440.207
8000160003200064000128000256000blankpool
23-Oct10-811-111-2
400.4890.5680.7010.9330.380.4080.6340.9720.4270.5170.771.061
800.4860.3980.6190.8810.2380.2760.5170.8940.2850.3680.6451.027
1600.3280.2190.460.8320.1160.1550.3360.8510.1550.2320.4921.024
3200.2150.1320.330.7410.0690.10.2150.7550.0950.1390.30.968
6400.1250.0760.1990.6070.0450.0640.1260.6180.0620.0860.1750.955
12800.0810.0510.1210.4450.0310.0430.0780.4590.0440.0580.1080.873
25600.0490.030.0720.2720.0210.0280.0450.2860.0310.0380.0660.737
IP0.8630.8240.7570.6850.5230.3640.2140.1170.0190.0210.1510.252
8000160003200064000128000256000blankpool
11-311-411-5
400.3530.4680.6740.9590.4590.5660.770.970.5810.5450.6691.081
800.2540.3280.560.9250.2960.4140.6490.9730.370.4430.5760.98
1600.1420.1810.3780.9130.1640.2440.4810.9470.2330.250.4190.926
3200.0880.1010.2130.8520.0860.1420.2830.9060.1290.1340.2440.81
6400.0530.0580.1160.8220.0510.0820.1570.8520.0710.0750.140.642
12800.0360.0410.0720.6960.0370.0540.0950.7910.0490.0490.0850.414
25600.0270.0290.0460.5660.0270.0350.0610.6840.0320.0330.0520.212
IP0.8550.8450.7870.7090.5830.3760.2340.1260.0190.0190.2550.165
8000160003200064000128000256000blankpool
11-611-711-8
400.2050.3210.5250.8690.2890.3470.4970.9010.3020.3830.7450.982
800.1460.2380.3960.8120.1890.2560.3870.8450.1870.2860.6160.96
1600.0840.1570.2850.7920.1250.1780.2880.840.1190.2080.4480.937
3200.0720.1180.190.7740.0830.1170.1890.8460.0750.1360.3070.976
6400.040.0560.1120.6790.0530.0680.1070.690.0530.080.1710.905
12800.0340.040.0580.5340.0320.0380.0590.4390.0350.0430.0830.659
25600.0240.0250.0470.5530.0280.0340.0480.40.030.0390.0620.772
IP0.8880.8270.830.7250.6220.3790.260.1620.0190.0190.2150.131
8000160003200064000128000256000blankpool
12-112-212-3
400.2460.2140.430.9720.370.420.5510.9170.3590.410.5460.928
800.1340.1440.3020.8980.2570.2920.4830.8430.2710.3060.4480.883
1600.0820.1190.2130.8590.1670.20.3640.7690.1880.2190.3480.823
3200.0580.0790.150.8010.1030.1330.2480.6370.1250.1450.2280.712
6400.0410.0520.0990.6510.0720.0860.1590.4490.0860.0890.1510.609
12800.0370.0520.0670.4640.0530.060.10.2890.0620.0590.0990.426
25600.0280.0290.0460.2780.0360.040.0640.1630.0420.0430.0620.256
IP0.9240.8960.870.7850.6950.4850.3240.1940.0220.0210.2670.099
8000160003200064000128000256000blankpool
12-412-512-6
400.4010.3880.7340.9940.3170.370.8240.9680.4930.5320.6520.853
800.2630.2670.6470.9930.2150.2680.7610.9660.3960.4010.4810.785
1600.1450.150.5150.9760.140.1670.6670.9730.2670.2910.4180.627
3200.0890.1280.3550.9340.090.1110.5240.9690.1680.1920.3030.461
6400.0630.0630.2170.8820.0580.0690.3540.9230.1020.0980.1980.239
12800.0510.0460.140.8140.0440.050.2150.9080.0740.0880.1340.126
25600.0370.0380.090.7590.0340.0370.1310.8720.0530.0550.0850.078
IP0.9440.8120.8880.7870.6290.4320.2980.1760.0160.0190.1280.132
8000160003200064000128000256000blankpool
23-Oct12-712-816-1
400.1790.3210.5970.9060.3560.3360.5220.8330.3330.3020.7530.39
800.1920.2820.5250.90.2430.2660.4650.7460.2440.270.6720.275
1600.1140.1370.3980.80.1530.1910.2770.6420.1660.2170.5360.173
3200.0850.1040.2960.7810.1050.1320.2370.4960.1160.1630.4240.098
6400.0550.0670.1790.6770.0650.0810.1540.2930.0760.1020.2590.073
12800.0370.0420.1120.6040.0440.0550.1010.1790.0560.0640.1760.04
25600.0270.0320.070.5420.0330.0410.0730.1050.0410.0490.1130.034
IP0.8340.7680.8170.6340.620.4440.2850.1780.0210.020.2010.103
8000160003200064000128000256000blankpool
16-416-716-8
400.2770.3390.6280.7770.3640.3680.6220.7440.4090.3360.5880.368
800.2440.2230.5860.640.2390.2740.4820.6330.2920.2420.4880.245
1600.170.1540.4890.5630.1510.1930.3950.4330.1940.1970.3620.147
3200.1050.1390.3560.5660.1060.1310.30.270.1240.1150.2420.089
6400.0770.0990.2250.4660.0620.0840.160.1570.080.070.1670.066
12800.0460.0390.1380.230.0460.0560.1210.0830.0550.0580.1090.036
25600.0320.0340.0810.1410.030.0370.0630.0470.0390.0310.0680.023
IP0.880.8220.8140.7140.5960.3950.2250.1580.0230.0190.1450.193
8000160003200064000128000256000blankpool
17-117-617-7
400.2790.2870.6970.8830.2470.2690.7520.9610.3380.4760.7120.99
800.2380.2230.630.7250.1630.2090.5920.8990.2410.3250.6160.923
1600.1420.1750.5350.7180.1290.1740.5180.9120.1820.280.530.874
3200.0990.1270.3890.680.0890.1260.470.9150.1250.1940.4030.902
6400.0640.0860.2880.5770.0590.0850.3020.8220.0780.1460.2790.833
12800.0440.080.1950.5060.0470.0630.1680.780.0610.0820.1670.707
25600.030.0570.1190.3370.0340.0460.1280.6340.0390.0590.1090.66
IP0.8570.8290.8150.7430.5690.4180.2080.1950.0250.0210.2150.19
8000160003200064000128000256000blankpool
17-818-118-2
400.190.2240.7240.8450.2710.3770.6080.7820.2390.3710.6780.318
800.1580.1810.6450.7890.190.2890.5570.7430.1850.2660.5790.226
1600.1110.1610.5730.7240.1470.2240.3990.660.130.2240.480.122
3200.080.1240.4670.6830.0950.1540.3110.5550.0880.1520.3280.076
6400.0490.0760.3210.5640.0690.1170.2390.3950.060.0930.2150.045
12800.0380.0440.2020.380.0490.0780.1720.2390.0480.0750.1450.033
25600.0280.0310.1060.2050.0310.0460.0830.1450.0330.0450.0960.019
IP0.8010.7650.750.6430.5820.3740.2160.1640.0180.0150.1720.163
8000160003200064000128000256000blankpool
18-318-418-5
400.1820.2380.5790.6740.1830.3220.6190.8560.330.2890.5930.822
800.170.2760.5260.6640.130.2440.5360.890.2490.2010.5460.776
1600.1110.1610.4540.550.0930.1690.4780.8580.1760.1320.4080.683
3200.0770.1190.3460.4060.0710.1210.2970.8160.1170.0870.290.503
6400.050.0830.2360.2910.0420.0860.210.7450.080.0770.220.432
12800.0350.0550.1520.1550.0290.0530.1130.670.0560.050.1390.255
25600.0220.0240.090.0690.0220.0350.090.5750.0360.0460.0890.122
IP0.7860.7710.7340.6640.5490.3620.2340.1370.0210.0120.1410.097
8000160003200064000128000256000blankpool
24-Oct18-718-82-1
400.0640.1250.3150.7260.0640.1270.3150.230.0830.1440.1360.088
800.050.0960.2340.5370.0520.1040.2270.1550.0670.1090.0940.055
1600.0330.0650.160.3280.0390.0780.1840.1030.0490.0860.0720.037
3200.0240.0530.1170.1870.0310.0590.1310.0550.0350.0610.0440.024
6400.020.0430.0840.1110.0230.0460.0940.0390.0280.0450.030.017
12800.0170.030.060.0570.0180.0310.0640.0290.0250.0350.0260.017
25600.0160.0230.0360.0310.0140.0230.0380.0170.0170.0240.0180.015
IP1.0611.0380.9530.8020.6840.4250.2560.1380.0130.0140.0580.084
8000160003200064000128000256000blankpool
7-17-27-3
400.0780.0950.1120.0490.050.0430.0840.0710.0480.070.1240.108
800.0530.0610.0820.0350.0380.0360.0640.0410.0340.0450.0890.065
1600.0370.0390.0550.0220.0260.0250.0430.0260.0220.0310.0630.048
3200.0280.0260.0370.0130.020.0180.0330.0170.0150.0210.0390.03
7-47-57-6
400.0710.0730.1120.0540.060.0720.1260.0730.0740.0660.1360.079
800.0570.0530.080.0290.050.0510.0890.0410.0580.0410.1030.052
1600.050.0370.0630.0210.0320.0370.0590.0340.0430.0410.0680.041
1.0070.9640.8550.6670.4660.240.1570.0870.0110.0140.0650.085
8000160003200064000128000256000blankpool
13-113-213-3
400.0640.110.3230.120.0750.1260.2790.1510.0580.1170.3370.246
800.0470.080.2460.080.0550.0960.2040.1110.0460.0930.2680.171
1600.030.0540.1690.0390.040.0660.150.0620.0340.0720.2020.109
3200.0240.0390.1260.0280.0290.0510.1120.0430.0250.0470.1310.054
13-413-513-6
400.0580.0990.2690.1160.0610.0890.2880.1390.0760.1460.3850.222
800.0420.0750.1980.080.0420.0670.2050.080.0530.1020.2670.148
1600.0320.0560.1390.0460.0320.0510.160.0490.0410.0790.2030.119
0.9850.9270.9190.8330.7220.4830.3390.1890.0090.0070.0570.071
8000160003200064000128000256000blankpool
2-22-32-4
400.0730.120.1320.0670.1030.110.1370.1150.0880.130.1650.127
800.0560.0780.0970.0420.0740.0830.1010.0730.0650.0890.1140.079
1600.0390.0540.0660.0290.050.0580.0720.050.0430.0630.080.048
3200.030.0430.0480.0210.0370.0430.0520.0370.0330.0440.0570.034
2-52-62-7
400.0720.1140.1660.1170.0570.0920.1060.1060.0790.1250.1310.123
800.0650.0870.1260.0750.0430.0660.0790.0620.0620.0920.0970.082
1600.0480.0640.0910.0470.0330.050.0570.0390.0440.0650.0660.056
0.7710.7980.8090.7680.6380.3630.2510.150.0130.0130.0670.078
8000160003200064000128000256000blankpool
8-18-28-3
400.060.0820.1360.0890.0550.0640.1460.8420.0620.0880.1260.067
800.0490.0610.1030.0510.0440.0460.1050.6720.0450.060.0880.047
1600.0280.0340.0620.0310.0290.0320.0670.460.0310.0430.0610.032
3200.0260.0290.0510.020.0250.0270.0580.2840.0250.0320.050.023
8-48-58-6
400.0820.0980.1270.6770.0590.0570.0970.1130.0870.1090.1590.199
800.0680.0660.0990.4510.0470.0430.0720.0630.0670.0780.1160.123
1600.0450.0320.070.2490.0330.0320.0490.0350.0480.0550.080.059
0.8880.8330.860.7650.5730.340.210.1110.0070.0070.090.107
8000160003200064000128000256000blankpool
14-114-214-3
400.0890.1080.1780.0840.0770.0660.1330.2770.0730.0840.1310.116
800.0520.0570.1370.0480.0520.0470.0880.1210.0480.0520.0890.072
1600.0360.0520.0940.0310.0370.0320.0620.0450.0310.0360.0610.041
3200.0260.030.070.0210.0280.0270.050.0340.0230.0280.0440.025
14-414-514-6
400.0850.0680.1490.0790.0550.060.1360.0840.0650.0740.1760.114
800.0630.0530.1080.0470.0370.0410.0870.0430.0440.050.1220.067
1600.040.030.0750.0280.0270.0250.060.0230.0320.0370.0830.036
0.8840.6680.7730.6510.4360.2330.1390.0620.0070.0050.0960.096
8000160003200064000128000256000blankpool
25-Oct10-110-210-3
400.1330.2210.3150.9290.1710.2480.3710.9110.1570.2540.3640.927
800.0980.1510.2130.9140.1210.1690.270.8710.1140.1760.2640.872
1600.0660.1080.1550.9280.0880.1160.1780.790.0750.1280.1850.73
3200.0470.0790.1120.8480.070.0750.120.6140.0580.0830.1320.464
6400.0370.0550.0720.6780.0470.0510.0830.3890.0450.0640.0880.3655
12800.0270.0410.0510.4270.0420.0350.0550.20.0360.0440.0690.141
25600.0220.0270.0340.2340.0290.0250.0370.1070.0280.0310.0410.078
IP1.0280.9540.8950.7930.6230.360.2340.1220.0150.0160.180.176
8000160003200064000128000256000blankpool
10-410-510-6
400.1730.1690.3240.9890.2020.1410.3531.0790.1540.2020.3661.114
800.1180.1110.21610.1350.0920.2341.0070.1080.1360.2521.015
1600.0840.0840.1551.0090.0990.0660.1610.9730.0730.0950.1730.913
3200.0550.0550.1060.8710.0650.0420.1070.8460.0520.0650.110.699
6400.0440.0420.0720.7750.0470.0290.0670.7690.0380.0420.0710.449
12800.0340.0330.0490.6360.0320.0250.0470.5980.0340.0310.0520.26
25600.0320.0270.0360.4220.0260.020.0290.3770.0220.0240.0330.136
IP1.1411.0751.0120.840.7930.5370.3360.1940.0160.0140.1870.191
8000160003200064000128000256000blankpool
10-710-811-1
400.1960.2330.3320.9860.2930.2260.5171.1090.1410.160.3711.154
800.1320.1560.2080.9090.2020.1520.3751.0470.1030.1140.2641.136
1600.0840.0940.1550.8640.1480.1090.2670.9120.0720.0830.1761.002
3200.0620.0610.1030.6750.0980.0740.1760.8810.0530.060.1191.015
6400.0460.0410.070.4240.0650.050.1180.6820.0410.0430.0780.835
12800.0350.0320.0490.2490.0480.0380.0790.4390.0380.0350.0570.571
25600.0260.0260.0340.1340.0360.0290.0550.2590.0310.0290.0440.345
IP1.0930.9171.0250.9010.7160.5220.3170.1950.020.020.1940.198
8000160003200064000128000256000blankpool
11-211-311-4
400.1330.1180.8681.0420.1220.1890.4171.0510.1640.2310.9421.197
800.0940.1080.5791.0420.0850.1370.2850.9950.1090.1540.6531.066
1600.0630.0640.3931.0040.0640.10.20.9850.0730.1090.3811.026
3200.0440.0350.2261.0120.0480.0690.1320.9920.0510.0740.2251.016
6400.0330.030.1370.9650.0330.0480.0870.9460.0430.050.1340.984
12800.0260.0220.0840.9080.0250.0370.0610.860.0320.0410.0790.847
25600.0230.0220.0520.7030.0220.0270.0410.6460.0230.0280.0510.644
IP0.9230.8280.9650.8390.6650.4470.2830.160.0190.0180.1940.208
8000160003200064000128000256000blankpool
11-511-611-7
400.1730.1230.2920.9370.1250.1810.5191.0640.1410.1840.3421.083
800.1080.0610.1930.9260.0850.130.30.8160.090.1210.2090.89
1600.0740.0440.1390.8630.0640.0980.2130.9060.0680.0890.1420.878
3200.0540.0360.0950.7980.0460.0690.1360.9150.0490.0660.0970.905
6400.0450.0350.0730.650.0380.0520.0980.9130.040.0530.070.797
12800.0350.0280.050.4060.030.0380.0620.7620.0350.040.0480.527
25600.0250.0150.0350.2120.0220.0280.0420.5520.0260.0330.0340.289
IP0.9050.7350.9020.7610.6630.490.2960.1780.0250.0250.2020.209
8000160003200064000128000256000blankpool
11-812-112-2
400.1630.1360.8810.9970.1060.1690.3950.9970.2190.2940.4981.089
800.1120.1040.740.9520.0690.1310.2420.9840.1480.2160.3860.917
1600.0760.0770.4850.9330.0570.0960.1490.9170.0990.1490.2740.8
3200.0550.0650.2830.8850.0440.0650.1040.8370.0690.0950.1770.53
6400.040.0450.1680.8430.0340.0490.0720.6530.0510.0630.1140.312
12800.0340.0320.1020.8470.0310.0410.0520.4120.0450.0510.0820.175
25600.0320.0190.0660.7330.0320.0330.0420.2440.040.040.0580.099
IP1.0631.0180.970.9050.7020.470.2820.1630.0310.0320.2450.24
8000160003200064000128000256000blankpool
12-312-412-5
400.2010.1580.4180.9750.1780.230.820.9240.1530.2140.8950.947
800.1270.1010.2980.8930.1210.1590.6540.990.0980.1350.7730.975
1600.0830.0890.2070.9130.0760.1080.4330.9390.0690.0930.5670.943
3200.0580.0530.1450.7930.0550.070.260.9460.0460.0580.3250.989
6400.0420.0340.0930.7280.0410.0510.1660.8820.0350.0410.2040.943
12800.0370.0260.0650.5260.0370.0370.0930.9120.0250.0340.1190.953
25600.0250.0210.040.2970.0210.0270.0550.7440.0220.0240.0670.854
IP0.9570.8990.8960.780.6660.4480.2620.1580.0940.0210.2080.209
8000160003200064000128000256000blankpool
12-612-712-8
400.2240.1730.4470.8280.1230.2120.460.9160.180.2180.4750.885
800.130.1210.3470.7750.0750.130.350.9670.120.1570.3870.801
1600.0620.0860.2440.5960.0580.1020.2150.9380.0670.1120.2960.647
3200.0480.0730.1640.3590.0490.0690.1390.8450.0540.0770.2020.417
6400.0330.0450.1080.1850.0340.0480.0910.7870.0360.0550.1310.235
12800.0290.0360.070.1030.0260.0350.0550.6430.0350.0370.0790.121
25600.0220.0190.0460.0590.0180.0280.0390.5160.0210.0290.0520.07
IP0.8610.7540.8660.8030.6860.5320.30.2050.0140.0180.1710.174
8000160003200064000128000256000blankpool
26-Oct9-19-29-3
400.2090.3580.8090.9610.2570.3650.4670.9780.2910.3220.5741.068
800.110.1920.620.940.1440.2330.2960.8870.1710.1930.3910.994
1600.0680.110.4260.9080.0870.1460.1880.7820.1050.1120.2460.923
3200.0460.0730.260.9030.0680.0870.1190.5710.0660.0740.1520.789
6400.0320.0460.1570.8130.0410.0570.0810.3280.0470.0460.0930.53
12800.0250.0330.0860.610.0290.0370.0490.1720.0330.030.0590.315
25600.0180.0210.050.3780.0180.0240.0310.0890.0230.0230.0390.17
IP0.9950.9690.8730.7770.6240.4060.2460.130.0120.0120.2220.205
8000160003200064000128000256000blankpool
9-49-59-6
400.1840.1880.6090.9770.1790.1510.3140.9390.2140.2710.4391.184
800.1010.1180.4110.8820.1110.090.2080.7580.1350.1650.2971.079
1600.0660.0740.2890.820.0790.0560.1360.540.0850.0930.1910.967
3200.0420.0430.1640.7840.0460.040.0880.3510.0520.0610.1120.933
6400.0290.0320.1050.6930.030.0280.0540.1910.0350.0350.0730.863
12800.0210.0230.0610.6060.0250.0230.0340.1090.0260.0250.0480.739
25600.0170.0140.0320.4250.0170.0150.0240.0630.0190.020.0320.523
IP0.9070.8840.850.7390.6110.4240.2470.1430.0110.0090.1830.194
8000160003200064000128000256000blankpool
9-79-816-1
400.3760.3580.5240.9020.1980.1850.360.9260.1920.2370.6070.27
800.2610.2350.3580.7410.1310.1180.2280.8140.1250.1680.4350.135
1600.1710.1540.2450.5920.0870.080.1530.5850.0880.1210.3140.077
3200.110.1040.1690.3770.0650.0580.1050.3250.0630.0870.2010.047
6400.0750.0650.1040.1960.0370.0390.0690.1690.0430.0540.1410.026
12800.0520.0460.0690.1120.0320.0290.0410.1050.0340.040.0880.023
25600.030.030.0480.060.020.0190.0280.0570.0230.0280.0590.017
IP0.9550.9230.920.8270.6550.4480.2630.1620.0140.0120.2030.179
8000160003200064000128000256000blankpool
5-15-25-3
400.2120.1620.8951.0410.2270.3070.4360.8770.2290.2681.0111.074
800.1110.0920.7351.0280.1390.1910.3090.6560.1240.150.8351.007
1600.060.060.5931.0480.090.1250.2110.4290.0760.0910.6981.08
3200.0350.0390.3641.0010.0590.0830.1380.2370.050.0580.4521.086
6400.0280.0290.2130.9620.0390.0530.090.1250.0330.0370.2591.045
12800.0210.0210.1250.8870.0280.0380.060.070.0260.0290.1441
25600.0120.0150.0690.8570.020.0240.0370.0390.0180.020.0781.004
IP0.9290.9260.9070.8170.6730.4450.2850.1560.010.010.1850.143
8000160003200064000128000256000blankpool
5-45-55-6
400.0990.1490.2640.1420.1360.1860.3490.7160.1760.2210.3450.788
800.050.0930.1660.070.0860.1160.2330.5490.1070.1360.2250.679
1600.040.0670.1120.0460.0610.0740.1610.4160.0670.0880.1520.586
3200.0250.0460.0720.0260.0420.0570.110.3260.0440.0570.0980.419
6400.0210.0280.050.020.0620.040.0790.1430.0330.040.0680.298
12800.0180.0230.0340.0190.0240.030.0510.0960.0290.030.0460.179
25600.0110.0170.0230.0120.0170.0210.0330.0650.020.020.0280.096
IP0.8370.9120.8850.7550.6350.4030.2340.120.0130.0140.2230.135
8000160003200064000128000256000blankpool
5-75-86-4
400.0990.1780.7791.0290.130.1940.6560.9690.1610.2170.6390.677
800.0490.1030.5460.9960.0780.1240.4481.0390.1030.1350.4680.618
1600.0380.0690.3051.0060.0550.0820.3040.9050.070.0930.3110.534
3200.030.0460.1651.0190.0420.0580.1920.8940.0510.0630.2140.365
6400.0210.0340.1150.9890.0270.0370.1240.8610.0370.0410.120.264
12800.0210.0270.0690.9570.0210.0280.0780.8220.0290.030.0830.223
25600.0160.0190.0440.8820.0150.0180.0430.7780.0180.0210.0480.155
IP0.8270.8690.8480.7910.6540.3860.2660.110.0130.0140.1660.114
8000160003200064000128000256000blankpool
6-16-26-3
400.10.2040.3390.1380.1210.2390.2250.0510.1540.2290.3530.569
800.0660.1240.2150.0670.0750.1360.1460.0240.0950.160.2230.375
1600.0280.0720.1470.0460.0540.0890.10.0170.0690.0950.220.371
3200.030.0560.1050.0280.0410.0660.0710.0120.0480.0660.0930.159
6400.0190.0350.0720.0210.030.0470.0450.0110.0340.0410.0970.106
12800.0180.0270.050.0190.0220.0330.0330.010.0290.0310.0610.054
25600.0120.020.0340.0140.0170.0240.0220.0090.0190.0230.0380.031
IP0.8730.910.8890.8080.6820.4250.2850.1370.0110.0130.2260.113
8000160003200064000128000256000blankpool
GST-IgG
5-45-55-618-Oct4-14-24-3
400.2680.3010.4560.2070.2560.3540.5230.9410.3430.3870.4950.942400.3920.3640.4730.6950.2820.3210.5170.2370.380.3430.5320.302
800.2080.1930.3320.1120.170.2240.3760.8520.2260.2470.3750.849800.2910.240.370.6020.2010.2270.2750.190.2790.240.420.19
1600.1030.1130.2160.0550.0970.1220.2490.8350.1380.1430.2450.7671600.1730.1540.2550.4170.1270.1630.2560.1290.1830.150.2950.106
3200.0780.0650.1360.0280.0620.0770.1630.7280.0780.0870.1550.6693200.0950.0920.1540.2460.0770.1030.1620.0850.1060.0960.1810.063
6400.050.0450.0820.0220.0410.0510.0990.6370.050.0510.0910.5316