BioNordika Finland Oy • tel 0207 410 270 • info@bionordika.fi • www.bionordika.fi November - December 2019 Issue 3 Happy birthday NEBNext! NEB’s NGS-dedicated product line, NEBNext, is turning 10 years this year! To celebrate our pre-teen we are giving out birthday cakes for THREE WINNERS parcipang in NEBNext-inspired crossword puzzle! 1. 1. In which kit fragmentaon, dA-tailing, end-repair and cleanup happens in one tube? 2. If bisulfite treatment is too harsh for your whole genome methylaon studies, which other opon could you choose? 3. You have a blood sample you want to sequence but want to get rid of globin mRNA and rRNA first. Which kit do you choose? 4. Which element makes it possible to make cDNA library out of single cells or very low inputs? 5. Which problem is small RNA library prep kit from NEB baling against? 6. What makes NEB’s adaptor so unique? 7. In which RNA workflow the input amounts range from 10 ng to 1 ug? 8. Which long-range fragment sequencer is supported by NEB reagents? 9. How can you separate molecular duplicates in NEBNext Direct kit? 10. Where in the manual will you find the info on how many standards you have in the E7630, NEBNext® Library Quant Kit for Illumina®? Let Anniina (anniina.brofeldt@bionordika.fi) know the correct answers to the crossword puzzle by end of December, and you might get a cake delivered to your office! 2. 3. 4. 5. 6. 7. 8. 9. 10. FREE SAMPLES for tesng All NEBNext products -10%
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BioNordika Finland Oy • tel 0207 410 270 • [email protected] • www.bionordika.fi
November - December 2019
Issue 3
Happy birthday NEBNext!
NEB’s NGS-dedicated product line, NEBNext, is turning 10 years this year!To celebrate our pre-teen we are giving out birthday cakes for THREE WINNERS
participating in NEBNext-inspired crossword puzzle!
1.
1. In which kit fragmentation, dA-tailing, end-repair and cleanup happens in one tube?
2. If bisulfite treatment is too harsh for your whole genome methylation studies, which other option could you choose?
3. You have a blood sample you want to sequence but want to get rid of globin mRNA and rRNA first. Which kit do you choose?
4. Which element makes it possible to make cDNA library out of single cells or very low inputs?
5. Which problem is small RNA library prep kit from NEB battling against?
6. What makes NEB’s adaptor so unique?
7. In which RNA workflow the input amounts range from 10 ng to 1 ug?
8. Which long-range fragment sequencer is supported by NEB reagents?
9. How can you separate molecular duplicates in NEBNext Direct kit?
10. Where in the manual will you find the info on how many standards you have in the E7630, NEBNext® Library Quant Kit for Illumina®?
Let Anniina ([email protected]) know the correct answers to the crossword puzzle by end of December, and you might get a cake delivered to your office!
2.
3.
4.
5.
6.
7.
8.
9.
10.
FREE SAMPLES for testing
All NEBNext products
-10%
Get your answers from our seminar BioNordika and Eurogentec will arrange a free seminar concerning the changing regulation and help around it. The seminar will be held in Helsinki in January with the title “A manufacturing partner for IVDR"
If you want to participate or have any questions, Anniina is happy to help via email or phone.
2 Valid until 31.12.2019 with promo code BN-WINTER.
Let's talk ...about getting the best start
New PhD student, is that you?Congratulations on your excellent decision to start a PhD thesis!
To make the beginning of your journey smoother, Bionordika wants to offer you a new student starter pack when signing up for our free newsletter.
Here are the instructions for ordering:1. Go to: https://www.bionordika.fi/tilaa-uutiskirje 2. Click which techniques interest you (to personalize the newsletter)3. Write to the comment field the following info: You are a new PhD student + your group and PI name4. Give your contact info5. Wait eagerly the new student starter pack to arrive.
IVDR is coming – brace yourself
Ask Anniina for more information!Anniina [email protected]. 020 7410 279
What is LDT?
Can I keep my in-house
PCR/qPCR assay?
How much work there is to
keep my in-house assay?
Can someone help me
with that?
I wonder if I have overdone
my gearfor this?
Valid until 31.12.2019 with promo code BN-WINTER 3
Let's talk ...about nucleic acid gel electrophoresis
Do you have a favorite ladder you are using every year? Maybe you remember even the ordering number or the band sizes?
Ladders may not be the most exciting products that you are working with, but you can save money by checking the price and how much product you are using per well. A good ladder is like a loyal friend, the one that never fails you!
We offer DNA, RNA and protein ladders, both prestained and unstained options.
NEB Ladders
Easier and safer tools Protein Ark Agarose tablets and non-carcinogenic DNA stain
End of year sale!
Protein Ark Agarose tablets:• Ready-to-Use tablet format: No messy weighing and improved gel-to-gel reproducibility• Prepare your gel in minutes, all you need is running buffer• Ultrapure Agarose: High resolution of DNA fragment with low background Magnite Green DNA stain:• Safer, non-carcinogenic alternative to Ethidium Bromide• Detect bands under Blue light or UV illuminator
BioNordika Standard Agarose is ideal for identification and recovery of roughly >1000 bp nucleic acid fragments by electrophoresis.
• DNAse and RNAse free• Sharp resolution• Strong gels with low background
Cat. no. Description OfferBN-50004 Standard Agarose – Type LE, 500 gram 250 €
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Valid until 31.12.2019 with promo code BN-WINTER.
Let's talk ...about updating your lab gear
Cat. no. Description OfferBN-50004 Standard Agarose – Type LE, 500 gram 250 €
Need new tools for your research? Get a spinner for free! • Is your centrifuge getting tired?• Do you have enough capacity in your shaker?• Do you need another dry bath?
siRNA with the new, unique RNAi-cap® technology • Stronger gene knockdown (up to 5-fold)• Reduced off-target effects• Not dependent on the algorithm• Longer gene knockdown (up to 12 days)• Protects from degradation in serum (up to 72h)• Gene knockdown guarantee!• Endotoxin-free CLINIgrade version for in vivo use
iBONi siRNA controls: validated with RNAi-cap®Test the difference! NEW!
Purchase new instruments from our suppliers Labnet International and Cleaver Scientific, worth for >1000 € and get a
spinner centrifuge FREE OF CHARGE!*
Co-Mix Combined Mixer and Vortexer
The only instrument to offer simultaneous microplate mixing and tube vortexing in one compact design
Description The Vitl Co-Mix is the only instrument to offer simultaneous, independently controlled microplate mixing and tube vortexing in one compact design.
The Co-Mix supports 96 and 384 well microplates in skirted, semi-skirted and unskirted variants plus deep-well plates, 0.2ml PCR tubes and PCR strips as well as 0.5, 1.5 and 2.0ml micro test tubes.
The Vortexer supports most skirted and unskirted tube formats.
Benefits
Intuitive touch-screen user interface Effortless changing between operating modes Pre-programmed with commonly used mixing programs User-defined mixing programs can be added to suit your needs Innovative plate-holding mechanism to avoid sample spillage or
contamination when loading and unloading microplates
Accessories
The Co-Mix also comes complete with a full set of adapter plates as standard.
Extra adapter plates can be purchased separately.
Technical Specifications
Dimensions (mm) W x D x H 190 x 350 x 200
Weight 7kg
Speed range (mixer) 200 – 3000 RPM
Speed range (vortexer) 700 – 3500 RPM
Time range (mixer) 5 seconds – 28 days
Time range (vortexer) 2 seconds – 60 seconds
Preset programs (mixer) 9
Preset programs (vortexer) 1
User defined programs Memory for 190 additional
user programs
Power Supply 100 – 240 VAC nominal
Catalogue number
V101001 Vitl Co-Mix (adapter plates included)
Please contact [email protected] for ordering details for the Co-Mix T: +44 1233 638 383 UK
T: +1 804 381 0905 USA
vitlproducts.com *One per order
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Let's talk ...about substrates for IHC
Valid until 31.12.2019 with promo code BN-WINTER.
Get the brightest colors in your IHC with Vector ImmPACT substrates!
Plus IHC kits include:• The normal kit reagents in Ready-To-Use (R.T.U.) format • BLOXALL® endogenous enzyme quenching solution (SP-6000)• ImmPACT® DAB EqV substrate (SK-4103)
Get added convenience with optimized and matched reagents in one product.
Cat. no. Description OfferMP-7800 ImmPRESS® Universal PLUS Polymer Kit, HRP
(Horse Anti-Mouse/Rabbit IgG), 15 ml218 €
MP-7801 ImmPRESS® Horse Anti-Rabbit IgG PLUS Polymer Kit, HRP, 15 ml
218 €
MP-7802 ImmPRESS® Horse Anti-Mouse IgG PLUS Polymer Kit, HRP, 15 ml
218 €
PK-8200 VECTASTAIN® Elite® ABC Universal PLUS Kit, HRP (Horse Anti-Mouse/Rabbit IgG), 50 ml
...and other ImmPACT Substrates: ImmPACT DAB EqV HRP (Cat# SK-4103), ImmPACT SG HRP (Cat# K-4705), ImmPACT AEC HRP (Cat# SK-4205), ImmPACT AMEC Red HRP (Cat# SK-4285)
Vector Laboratories ImmPACT™ line of substrates offer the following advantages:• 2-4 fold greater sensitivity than the original substrate kits• Supplied with stock solutions in dropper bottles and optimized diluent• Optimized specifically for immunohistochemistry applications• Stock solutions stable for one year, working solution stable for up to 14 days at 4 ºC
All ImmPACT substrates
-14%
ImmPACT DAB HRP #SK-4105
ImmPACT VIP HRP #SK-4605
ImmPACT NovaRED HRP #SK-4805
ImmPACT Vector RED AP#SK-5105
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Let's talk ...about protein purification
Valid until 31.12.2019 with promo code BN-WINTER
Protein Ark’s HiFliQ 1 ml and 5 ml FPLC columns come pre-packed and ready for use with pre-charged Heparin, Nickel-NTA, Cobalt-NTA and Glutathione Agarose resin for affinity purification of Heparin-binding protein, poly-histidine and GST-tagged recombinant proteins.
HiFliQ columns are compatible with all common chromatography instruments (including ÄKTA™ FPLC’s) and a wide range of reducing agents, detergents and other buffer additives.
Pre-packed HiFliQ FPLC Columns
Name Resin Column capasity Units1 ml HiFliQ Ni-NTA FPLC column Ni-NTA 50-75 mg 1 x 1 ml, 5 x 1 ml
5 ml HiFliQ Ni-NTA FPLC column Ni-NTA 250-300 mg 1 x 5 ml, 5 x 5 ml
1 ml HiFliQ GST FPLC column Glutathione 10 mg 1 x 1 ml, 5 x 1 ml
5 ml HiFliQ GST FPLC column Glutathione 50 mg 1 x 5 ml, 5 x 5 ml
1 ml HiFliQ Co-NTA FPLC column Co-NTA 40-50 mg 1 x 1 ml, 5 x 1 ml
5 ml HiFliQ Co-NTA FPLC column Co-NTA 200-250 mg 1 x 5 ml, 5 x 5 ml
1 ml HiFliQ HeparinFPLC column Heparin 3-5 mg 1 x 1 ml, 5 x 1 ml
5 ml HiFliQ HeparinFPLC column Heparin 15-20 mg 1 x 5 ml, 5 x 5 ml
Simply load your culture media onto the column!HiFliQ Ni Advance FPLC Columns (1 ml & 5 ml) are designed for faster purification of secreted proteins using clarified culture media directly. The Fastback Ni Advance resin resistant to EDTA (up to 20 mM) and DTT (up to 20 mM) for IMAC chromatography.
There is still time to take advantage of Cell Signaling Technology (CST) B3G4 campaign. Choose 4 products from CST’s entire portfolio of primary and secondary antibodies, antibody sampler kits, ELISA kits, SimpleChIP kits and companion products. We’ll give the cheapest item for FREE!Need help choosing? Contact our antibody specialist Juho!
The best antibody deal of the year!
Only valid until 13.12.2019
Endothelial cells are often used to study f.ex. angiogenesis and it’s role in the develop-ment of cancer and metastasis, cardiovascular diseases and blood brain barrier models• Human umbilical vein endothelial cells (HUVEC)• EGMTM Endothelial Cell Growth Media family
Hepatocytes are commonly used in drug development phases but also in the study of non-alcoholic fatty-liver disease• Plateable human hepatocytes• Hepatocyte culture BulletKit, Thawing medium, Plating medium
Neuronal cells are useful for the study of pathogenesis in the neurodegenerative diseases• Especially rodent neurons and glial cells• PNGM Primary Neuron Growth Medium, AGM Astrocyte Growth Medium
Hematopoietic cells can feel like easy-to-obtain but, in fact, can be difficult to isolate and characterize. Wide variety of different hematopoietic cells can be used to study f.ex. immunotherapies and cell therapy.• PBMCs, CD34+ cells• X-Vivo media
BioNordika Finland Oy • Kutomotie 18, 00380 Helsinki tel 0207 410 270 • [email protected] • www.bionordika.fi
Promo code: BN-WINTER19Note! The promotional code must be referenced when ordering to receive the
discounts and special prices. The offers are listed ex. VAT and shipping charges, and are valid until 31.12.2019. The offers cannot be combined with other offers
or agreements. We reserve the right for typos.
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Let's talk ...about biologically more relevant cell culture
Primary cells do better with optimized set of medium and growth factors
Your benefits:• Massive pool of donors available - get the right tools for your research• Easy to source just when you need the cells• Strict Quality Control and guaranteed performance
20% discount d
when a vial of cells* is purchased together with an optimized Bullet Kit for cultivation!