Guidance for Submission of AMR Surveillance Isolate for External Quality Assessment and Reporting Emerging AMR Alerts National AMR Surveillance Network NCDC National Programme on Containment of Antimicrobial Resistance National Centre for Disease Control, New Delhi March 2019
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Guidance for Submission of AMR Surveillance Isolate for External … · 2019. 4. 24. · i. The specimens or isolates of priority AMR pathogens are covered by UN 3373 shipment procedures
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Guidance for Submission of AMR Surveillance Isolate for External
Quality Assessment and Reporting Emerging AMR Alerts
National AMR Surveillance Network
NCDC
National Programme on Containment of Antimicrobial Resistance National Centre for Disease Control, New Delhi
March 2019
1 | P a g e
Table of Contents
I. Expectation from AMR surveillance laboratories ................................................................................................. 2
II. Preparation of nutrient agar media stabs ............................................................................................................... 2
III. AST results reporting ............................................................................................................................................ 3
IV. Standard Precautions: ............................................................................................................................................ 3
V. List of strains to shipped to NCDC for External Quality Assessment (EQAS) .................................................... 4
VI. Isolate packaging and transportation according to International Air Transport Association’s Dangerous
VIII. List of antibiotics to be tested for priority bacterial pathogens before shipping the isolates to NCDC
Organism
Sp
ecim
en
Antibiotics
Am
ika
cin
30
μg
Am
pic
illi
n 1
0 μ
g
Azi
thro
my
cin
15
μg
Cef
epim
e 3
0 μ
g
Cef
ota
xim
e 30
μg
Cef
ox
itin
30 μ
g
Cef
tazi
dim
e 30
μg
Cef
tria
xo
ne
30
μg
Ch
lora
mp
hen
icol
30
μg
Cip
rofl
ox
aci
n 5
μg
Cli
nd
am
yci
n 2
μg
Do
xy
cycl
ine
30
μg
Ery
thro
my
cin
15μ
g
Gen
tam
icin
10
μg
Gen
tam
icin
Hig
h 1
20
μg
Imip
enem
10
μg
Lin
ezo
lid
30
μg
Min
ocy
clin
e 30
μg
Nit
rofu
ran
toin
300
μg
Pip
era
cill
in/T
azo
ba
ctu
m
10
0/1
0 μ
g
Tet
racy
clin
e 3
0μ
g
TM
P/S
MX
*
Va
nco
my
cin
30μ
g
Va
nco
my
cin
scr
een
Ag
ar
Co
list
in M
IC
Va
nco
my
cin
MIC
Comments
Enterococcus species B P O**
✓
✓ ✓
✓
✓
✓
Enterococcus species U ✓
✓
✓ ✓
✓
✓
Staphylococcus aureus B, P,O
✓
✓ ✓ ✓ ✓ ✓
✓
✓
✓
✓
Escherichia coli B, P, O
✓
✓ ✓
✓
✓
✓
✓
Escherichia coli U
✓
✓ ✓
✓
✓
✓
✓
Klebsiella species B, U, P, O
✓ ✓
✓
✓
✓
✓
Acinetobacter species B, U, P, O
✓ ✓
✓
✓ ✓ ✓
✓
✓
Pseudomonas species B, U, P, O
✓ ✓
✓
✓
✓
✓
✓
Salmonella enterica
serotype Typhi B
✓ ✓
✓ ✓ ✓
✓
✓
Salmonella enterica
serotype Typhi S
✓
✓ ✓ ✓
✓
✓
Salmonella enterica
serotype Paratyphi B
✓
✓ ✓ ✓
✓
✓
*TMP/SMX- Trimethoprim Sulfamethoxazole 1.25/23.75 µg; **B – Blood, U- Urine, O – Other sterile body fluids, S- Stool. All tick marked (✓) antibiotics to