Green and chemical synthesized ZnO nanoparticles on germinating Sesamum indicum (Co- 1) and their antibacterial activity Narendhran. S 1 *, P. Rajiv 1 and Rajeshwari Sivaraj 2 1 Department of Biotechnology, School of Life Sciences, Karpagam Academy of Higher Education, Eachanari post, Coimbatore 641 021, Tamil Nadu, India. Tel./fax: +91 422 647 1113 2 Department of Chemistry, Government arts college, Udumalpet *Corresponding author: [email protected]Abstract A Comparative study of chemical (ZnO) and biological synthesized (nano-ZnO) nanoparticles were carried out in order to determine effect on seed germination of Sesamum indicum (Co-1) by soaking method. Nano-ZnO synthesized by using Lantana aculeata aqueous extract. Chemical synthesis of ZnO nanoparticles by precipitate method and was characterized by Ultra Violet–visible spectroscopy (UV–vis), Fourier transform infrared spectrometer (FT-IR), Energy dispersive X-ray spectrometer (EDX), X-ray diffractometer (XRD), Field emission scanning electron microscopy (FESEM), High Resolution Transmission electron microscopy (HRTEM). Antibacterial activity against pathogens were determined using well diffusion method. All the characterization analysis revealed that ZnO & nano ZnO nanoparticles were spherical in shape with an average particle size of 18 ± 2 nm and 12 ± 3 nm. Antibacterial studies conclude that nano-ZnO NPs has maximum zone of inhibition which was observed in P.aeruginosa (15.60 ± 1.0 mm) at 100 μg /ml concentration when compared to other ZnO NPs. Phytomediate ZnO have no adverse effects on seed germination, root elongation on Sesamum indicum. But chemical synthesized ZnO nanoparticles significantly decreased in germination of Sesamum indicum treated samples and no changes were observed in Bulk ZnO. These results clearly indicate the benefits of using bio fabricate ZnO nanoparticle is more efficient in germination of Sesamum indicum and can also act as antibacterial agent. It can be used as nanofertilizer in environmental aspect of agricultural development. Keywords: Antibacterial activity, Seed germination, Sesamum indicum, L. aculeata, ZnO nanoparticles
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Green and chemical synthesized ZnO nanoparticles on germinating Sesamum indicum (Co-
1) and their antibacterial activity
Narendhran. S1*, P. Rajiv1 and Rajeshwari Sivaraj2 1Department of Biotechnology, School of Life Sciences, Karpagam Academy of Higher
Education, Eachanari post, Coimbatore 641 021, Tamil Nadu, India. Tel./fax: +91 422 647 1113 2Department of Chemistry, Government arts college, Udumalpet
In current study, the chemically synthesized ZnO NPs were characterized by different
techniques for calculation of shape, particles size and morphology. The different concentrations
of nano-ZnO & ZnO nanoparticles effect on germination and root elongation of sesamum seed
by soaking method were studied. Metal oxide are quickly transported through the plant and
included in the metabolic processes through soaking methods. We observed that sesamum seeds
germination at lowest concentration (0.1, 0.25, 0.5 g/L) of nano-ZnO suspension solution proved
good root growth compared to ZnO NPs and control. In antibacterial assay, nano-ZnO NPs
shows maximum inhibition against all pathogen and so it can be effectively used as antibacterial
agent and nano fertilizer in environmental aspect of agricultural development. Therefore, the
challenge for further studies is to uptake the kinetics and interaction mechanisms within cells,
also the maximum amenable amount of these nanoparticles which plants can take without
showing any signs of stress.
5. Acknowledgements
We thank to Management of Karpagam Academy of Higher Education, Coimbatore,
Tamil Nadu, India for providing necessary facilities to carry out this work.
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