Gram stain • Technique used to differentiate two large groups of bacteria based on their different cell wall constituents. ( peptidoglycan ) • Are they Gram positive or negative? • What is the morphology (rod, coccus, spiral, pleomorphic [variable form] etc)? • Do cells occur singly or in chains, pairs etc? • How large are the cells?
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Gram staining, Culture , Antimicrobial sensitivity test
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Gram stain
• Technique used to differentiate two large groups of bacteria based on their different cell wall constituents. ( peptidoglycan )
• Are they Gram positive or negative? • What is the morphology (rod, coccus, spiral,
pleomorphic [variable form] etc)? • Do cells occur singly or in chains, pairs etc? • How large are the cells?
N. Meningitidis
N. Meningitidis
H.influenzae
Streptococcus agalactae
Microbiological Culture
• Multiplying microbial organisms by letting them reproduce in predetermined culture media under controlled laboratory conditions.
Culture media
Solid vs broth Reducing
Non-synthetic vs chemically defined Selective
Differential
S.pneumoniae
H.Influenzae in chocolate agar
N.Meningitidis
Antibiotic sensitivity test
• Laboratory test determines how effective antibiotic therapy is against bacterial infections.