Gram Staining

Microbiology laboratoric test

Gram Stain Material

solutions of crystal violet, Gram’s iodine (2 g potassium iodide in 300 ml distilled water plus 1 g iodine crystals), 95% ethanol and/or

isopropanol-acetone mixture (3:1 v/v), and

safranin Bismark brown stain (for color-

blind students) clean glass slides inoculating loop Bunsen burner bibulous paper

microscope lens paper and lens cleaner immersion oil Hyphomonas

(Hyphomicrobium) neptunium (ATCC 15444) grown in marine broth (Difco)

slide warmer staining rack Bacto Gram Stain Reagents

from Difco for the three-step Gram stain

Gram Stain

Gram Stain

Gram Stain

Gram Stain

Gram Stain

Gram Stain

Gram Stain

Gram Stain

Gram Stain

Gram Stain

Examination the preparation under microscope ( x100) for confirmation

Microscopic result for Gram Stain