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General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.
• Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal
If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.
Downloaded from orbit.dtu.dk on: Jun 14, 2018
Glycoproteins at the rubbing interfaces of biosystems
Lee, Seunghwan
Publication date:2010
Document VersionPublisher's PDF, also known as Version of record
Link back to DTU Orbit
Citation (APA):Lee, S. (2010). Glycoproteins at the rubbing interfaces of biosystems [Sound/Visual production (digital)]. 4thProtein.DTU Workshop, Technical University of Denmark, 01/01/2010
L. Shi and K.D. Caldwell, J. Colloid & Interf. Sci. (2000) 224, 372‐381
Suppression of proteins and bacteria adsorptionSuppression of proteins and bacteria adsorption L. Shi, R. Ardehali, P. Valint, & K.D. Caldwell, Biotech. Letters (2001) 23, 437‐441
LubricationI.C.H. Berg, L. Lindh & T. Arnebrant, Biofouling (2004) 20, 65‐70
S. Lee, M. Müller, K. Rezwan, N.D. Spencer, Langmuir (2005) 21, 8344‐8353
h h l l
2HN COOH
Mucins as a amphiphilic copolymer
‐CH2CH2O‐ ‐CH2CH2O‐‐CH2CHO‐
m mnCH3
m mn
Model surface and pin‐on‐disk tribometry
pin‐on‐disk tribometerElastomer as model surface of biological i h l
loaddead
tissues: mimic mechanical properties
lever
Pin (PDMS)
deadweight
PGM-containingYoung´s modulus
Poly(dimethylsiloxane) (PDMS)
aqueous solutionYoung s modulus
ca. 2 MPa
Poission ratio
rotationalmotion
disk (PDMS)
L d 1 N
Poission ratio
0.5
side view
Load = 1 N
P ~ 0.5 MPa
Lubrication properties of PGM solution: pH 7
Lubrication properties of PGM solution: pH 2
Lubrication properties of PGM solution: pH 12
Optical Waveguide Lightmode Spectroscopy (OWLS)
Surface adsorption properties: OWLS
Optical Waveguide Lightmode Spectroscopy (OWLS)
Adsorption of mucins onto PDMS surface dso pt o o uc s o to S su ace
*PDMS (~30nm)
Waveguide(SiOx0.75TiOx0.25)
TM TE
incidence angle
Surface adsorption properties: OWLS
300
250
PGM ( H 2)m2 )
150
200 PGM (pH 2)PGM (pH 7)PGM (pH 12)
mas
s (n
g/cm
100
Adso
rbed
m
0
50
0 5 10 15 20 25 30 35 40
buffer rinsingA
0 5 10 15 20 25 30 35 40time (minutes)
PGM, 1mg/ml buffer: 1mM KH2PO4, KCl 0.1M
Surface adsorption properties: OWLS
pH and ionic strength dependence
300pH 2pH 7
H 12ng/c
m2 )
200
pH 12
d m
ass
(n
100
Ads
orbe
d
00 0001 0 001 0 01 0 1 1 10
A
0.0001 0.001 0.01 0.1 1 10
total ionic strength (M)
Tertiary structure: Near‐UV CD spectroscopy
pH dependence
20
30pH2pH4pH7
pH 7
0
10pH10pH12
-20
-10pH 2
-30250 270 290 310 330 350
wavelength (nm)
disruption of tertiary structure of “naked” polypeptide region