US 20020150968A1 (12) Patent Application Publication (10) Pub. No.: US 2002/0150968 A1 (19) United States Wang et al. (43) Pub. Date: Oct. 17, 2002 (54) GLYCOCONJUGATE AND SUGAR (52) US. Cl. ........................... .. 435/53; 435/681; 435/96; NUCLEOTIDE SYNTHESIS USING SOLID 435/175 SUPPORTS (76) Inventors: Peng G. Wang; Troy; MI (US); Xi Chen; NorristoWn; PA (US) Correspondence Address: Brinks Hofer Gilson & Lione PO. Box 10395 Chicago, IL 60610 (US) (21) Appl. No.: 09/757,846 (22) Filed: Jan. 10, 2001 Publication Classi?cation (51) Int. Cl.7 ......................... .. C12P 33/20; C12P 21/02; C12P 19/20; C12N 11/18 (57) ABSTRACT This invention relates to methods and compositions for the in vitro production of glycoconjugates. In particular, a preferred production system is provided that comprises a solid support, at least one sugar nucleotide producing enZyme; at least one glycosyltransferase; at least one bioen ergetic; and at least one acceptor. The sugar nucleotide producing enZyme(s) is preferably immobilized on the solid support. The glycosyltransferase may be co-immobiliZed on the solid support With the sugar nucleotide producing enZyme(s), or may be provided to the solid support in solution.
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Glycoconjugate and sugar nucleotide synthesis using solid supports
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US 20020150968A1
(12) Patent Application Publication (10) Pub. No.: US 2002/0150968 A1 (19) United States
Wang et al. (43) Pub. Date: Oct. 17, 2002
(54) GLYCOCONJUGATE AND SUGAR (52) US. Cl. ........................... .. 435/53; 435/681; 435/96; NUCLEOTIDE SYNTHESIS USING SOLID 435/175 SUPPORTS
(76) Inventors: Peng G. Wang; Troy; MI (US); Xi Chen; NorristoWn; PA (US)
This invention relates to methods and compositions for the in vitro production of glycoconjugates. In particular, a preferred production system is provided that comprises a solid support, at least one sugar nucleotide producing enZyme; at least one glycosyltransferase; at least one bioen
ergetic; and at least one acceptor. The sugar nucleotide producing enZyme(s) is preferably immobilized on the solid support. The glycosyltransferase may be co-immobiliZed on the solid support With the sugar nucleotide producing enZyme(s), or may be provided to the solid support in solution.