INERTSIL ® ODS-2 An HPLC column offering symetric peaks for polar bases and acids and low pressure. GL SCIENCES HPLC COLUMNS GL Sciences’ Original High Purity ODS Phase When Inertsil ODS-2 was introduced in the late 1980s, this HPLC transformed the entire industry. Inertsil ODS-2 was the first HPLC phase created using ultra high purity silica, which produced superior base deactivation. Additionally, a unique “oligomeric” bonding architecture created a novel selectivity and a degree of chemical and physical ruggedness as yet unseen. Until this phase was eclipsed by the performace of it’s sibling Inertsil ODS-3 (and now, by Inertsil ODS-4), it was GL Sciences’ most popular phase and continues to be used widely and reliably for long established methods in pharmaceutical and environmental labs. While Inertsil ODS-2 is generally recommended primarily for following prescribed methods, (Inertsil ODS-4 and ODS-3 being recommended for new methods) it constitutes of one of six Inertsil ODS phases offering alternative selectivities for polar compounds. For additional information, please visit www.inertsil.com. Features & Benefits • Extremely reproducible. • Oligomeric bonding yields unique selectivity • Standard dimensions always in stock. Phase Characteristics Particle Size: 5um Particle Shape: Spherical Specific Surface Area: 320m2/g Pore Size: 150A Pore Volume: 1.15mL/g Purity: 99.999% Bonded Phase: Octadecyl groups Endcapped: Yes Carbon Load: 18.5% USP: L1 Guaranteed Efficiency: 80,000 p /m Pure Reverse Phase Chromatography The absence of metals and other impurities in Inertsil ODS-2 silica, combined with robust end-capping allows pure reverse phase chromatography for even the most polar compounds. The chromatograms at left show selectivity differences between Inertsil ODS-2 and GL Sciences’ more advanced ODS phases. Selectivity Comparison between Inertsil ODS Phases using PAHs and Alkylbenzenes Columns: As indicated, 250 x 4.6mm; Eluent: MeCN: 25mM Phosphate Buffer, pH 7.0, 40:60 Flow Rate: 1.0 mL/min Temp: 40C Detecon: 210nm Sample: Uracil, Caffeine, Phenol, n-Butylbenzene, o-Terphenyl, n-Amylbenzene, Triphenylene ODS-4 ODS-3 ODS-SP ODS-2 ODS-EP ODS-P 1 +2 3 4 7 5 6 1 +2 3 4 7 5 6 1 +2 3 4 7 5 6 1 2+3 4 7 5 6 1 +2 3 4 7 5 6 1 +2 3 4 7 5 6 Inertsil ODS Phase Selectivity Comparison