Get published in Science, meet · regulation in such fields as molecular biology, development, immunology, neuroscience, microbiology, physiology and medicine, pharmacology, biochemistry,

Established and presented by:

Established in 1995, the GE & Science Prize for Young Life Scientists

brings science to life by recognizing outstanding Ph.D.s from

around the world and rewarding their research in the field of

molecular biology.

This is your chance to gain international acclaim and recognition

for yourself and your faculty, and to turn your scientific ideas

into reality.

• If you were awarded your Ph.D. in molecular biology* during

2007, describe your work in a 1000-word essay.

• Submit your essay for the 2008 GE & Science Prize for Young

Life Scientists.

• A panel of distinguished scientists will select one Grand Prize

winner and four regional winners.

• Submission deadline August 1.

Few awards in science are more rewarding than the Science/AAAS

and GE Healthcare-sponsored prize – just ask the Grand Prize

winner for 2007. In addition to having his essay published in

Science, Matthew Stremlau received $25,000, was flown to the

awards ceremony in Stockholm, Sweden, and got to pick the

brains of Nobel laureates Mario R. Capecchi, Sir Martin J. Evans

and Oliver Smithies.**

GE & Science Prize for Young Life Scientists:

Ph.D. Research Re-imagined.

For more information, go to

www.gelifesciences.com/science

Get published in Science, meetthe Nobel laureates, win a tripto Stockholm and $25,000.

* For the purpose of this prize, molecular biology is defined as “that partof biology which attempts to interpret biological events in terms of thephysico-chemical properties of molecules in a cell” (McGraw-Hill Dictionaryof Scientific and Technical Terms, 4th Edition).

**Nobel Prize 2007 winners in Physiology or Medicine for their discoveriesof principles for introducing specific gene modifications in mice by theuse of embryonic stem cells.

Grand Prize winner Matthew Stremlau presents his

research to a captivated audience

Prize winners with Nobel laureates Mario R. Capecchi,

Sir Martin J. Evans and Oliver Smithies.

Submission deadline

August 1

CELL BIOLOGY

CELL SIGNALING

DRUG DISCOVERY

IMMUNODETECTION

LAB WATER

PROTEIN BIOMARKERS

STEM CELL RESEARCH

We stand with you in our investment in and commitment

to stem cell research. By combining Upstate® and Chemicon®

products and services with our years of experience supporting

Life Science research, we successfully partner with the world’s

leading scientists to develop proven solutions for human and

murine, adult and embryonic stem cell research.

ADVANCING LIFE SCIENCE TOGETHER™

Visit www.millipore.com/stem_cells for more information on this

and other ways Millipore supports Life Science research.

NEXT GENERATION?

Give rise to controlled differentiation.

THE EXPERTISE OF

CHEMICON® & UPSTATE®

IS NOWA PART OFMILLIPOREMillipore, Chemicon and Upstate are registered trademarks of Millipore Corporation.

“M” logo and “Advancing Life Science Together” are trademarks of Millipore Corporation.

GE Healthcare

When it comes to life sciences, GE Healthcare is setting the

standard. Tens of thousands of scientists in over 100 countries

around the world rely on our products every day. We have

delivered more than 60 000 research protein purification systems,

1500 BioProcess™ systems and 12 000 BioProcess columns

worldwide. Our Amersham family of consumables, with its 60-year

heritage, is trusted to provide accurate results time and time again.

But we’re never content to stand still. We constantly strive for

new innovations for tomorrow’s research and drug development.

And the result is groundbreaking products like the ÄKTAdesign™

platform, IN Cell Analyzer, Ad-A-Gene Vectors, and MabSelect™

media. Thanks to our technological achievements and global

presence, we’re able to help you turn your scientific ideas into

reality – bringing science to life and helping transform healthcare.

We call it Life Science Re-imagined.

Discover more at www.gelifesciences.com

Bringingscience to life

GE

He

alt

hca

reB

io-S

cie

nce

sA

B,a

Ge

ne

ralE

lect

ric

Co

mp

an

y.B

jörk

ga

tan

30

,75

18

4U

pp

sala

,Sw

ed

en

.©

20

07

Ge

ne

ralE

lect

ric

Co

mp

an

y-

All

rig

hts

rese

rve

d.

GE

34

-07

Protein sample and assay technologies

by QIAGEN

Simplify complexity

Advanced Sample & Assay Technologies

Rely on QIAGEN protein sample and assay technologies for:

� Expression and cloning

� Expression-ready vectors for all human genes

� Protein fractionation and purification

� Protein assays

� Protein crystallization

� Mass spec sample preparation

Contact QIAGEN today or visit www.qiagen.com/goto/Protein .

Sample & Assay Technologies

PRO

TPortf0

508S1W

W

How can you protect yourself, the environment, and your DNA sample?

Answer: SYBR® Safe. Fact is, it’s just as effective as ethidium bromide, yet it’s not a hazard-

ous agent. So it’s not only better for the environment, but better for you. And since SYBR®

Safe uses blue light for visualization, your DNA sample won’t be damaged by UV, so you’ll

get better cloning efficiencies. Next time you’re staining DNA, be sure to make the safer

choice for yourself and the environment. Get SYBR® Safe.www.invitrogen.com/sybrsafe

2008 © Invitrogen Corporation. All rights reserved. These products may be covered by one or more Limited Use Label Licenses (see the Invitrogen catalog or our website, www.invitrogen.com).

Stain DNA,

not nature.

From the publishers of Science, Science Signaling, formerly known as

Science’s STKE, will add original research starting September 2008.

Each week the journal will publish leading-edge findings in addition

to the current features, including Perspectives, Reviews, Protocols,

Meeting Reports, Book Reviews, Teaching Resources, and the

Database of Cell Signaling.

Science Signaling showcases high-impact research in cellular

regulation in such fields as molecular biology, development,

immunology, neuroscience, microbiology, physiology and medicine,

pharmacology, biochemistry, cell biology, bioinformatics, and systems

biology. Submit your research that provides new concepts and new

understanding of biological signal transduction for consideration.

Subscribing to Science Signaling ensures that you and your lab have

the latest cell signaling resources. From basic science to design of

therapeutics, from molecules to networks and systems design, read

the best source – Science Signaling.

Submit your paper to:stke.sciencemag.org/about/help/research.dtl

ISSN: 1937-9145

— 10.0

— 5.0

;;— 3.0

— 1.5

— 0.5

NEW

ENGLAND

BIOLABS

For more information and international distribution network, please visit www.neb.com

the leader in enzyme technology

New England Biolabs Inc. 240 County Road, Ipswich, MA 01938 USA 1-800-NEB-LABS Tel. (978) 927-5054 Fax (978) 921-1350 [email protected]

Canada Tel. (800) 387-1095 [email protected] • China Tel. 010-82378266 [email protected] • Germany Tel. 0800/246 5227 [email protected]

Japan Tel. +81 (0)3 5669 6191 [email protected] • UK Tel. (0800) 318486 [email protected]

Looking for the right solution for your high yield PCR? Choose recombinantTaq DNA Polymerase

from New England Biolabs. As the leader in enzyme technology, New England Biolabs provides

the highest quality recombinantTaq at exceptional value. Our expanded selection ofTaq based

products includes kits, master mixes, and a choice of reaction buffers. ChooseTaq DNA polymerase

from NEB for guaranteed Performance, Convenience and Results.

yield of dreams.

Taq DNA Polymerase from New England BiolabsHIGH YIELD, ROBUST AND RELIABLE PCR REACTIONS IN CONVENIENT FORMATS

0.5 0.6 2 3 4 8 12 20 30 M

Amplification of specific sequences from human genomic DNA using

LongAmp Taq DNA Polymerase. Amplicon sizes are indicated above gel.

Marker M is the 1 kb DNA Ladder (NEB #3232).

LongAmp Taq now availablefor larger PCR products

n Taq with Standard Buffer r . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . M0273S/L/X

n Taq with Standard (Mg-free) Buffer r . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . M0320S/L

n Taq with ThermoPol Buffer r . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . M0267S/L/X

n Taq with ThermoPol II (Mg-free) Buffer r . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . M0321S/L

n Taq 2X Master Mix r . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . M0270S/L

n Taq 5X Master Mix r . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . M0285S/L

n Quick-Load ™ Taq 2X Master Mix r . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . M0271S/L

n Taq PCR Kits r . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . E5000S/E5100S

n LongAmp Taq DNA Polymerase r . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . M0323S/L

n LongAmp Taq PCR Kit r . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . E5200S

n LongAmp Taq 2X Master Mix r . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . M0287S/L

= Recombinantr

Amplicon Size (kb)

kb

In 2005, the Genome Sequencer 20 System was launched

� Read length: 100 bases

� 20 million bases in less than 5 hours

In 2007, the Genome Sequencer FLX System was launched

� Read length: 250 to 300 bases

� 100 million bases in less than 8 hours

Available in 2008, the Genome Sequencer FLX with improved chemistries

� Read length: >400 bases

� 1 billion bases in less than 24 hours

More applications lead to more publications.

Proven performance with an expanding list of applications and

more than 130 peer-reviewed publications.

Visit www.genome-sequencing.com to learn more.

Sequencing-by-Synthesis: Using an enzymatically

coupled reaction, light is generated when individual

nucleotides are incorporated. Hundreds of thousands of

individual DNA fragments are sequenced in parallel.

Longer sequencing readsmean more applications.

www.roche-applied-science.com

Genome Sequencer FLX System

For life science research only. Not for use in diagnostic procedures.

454 and 454 SEQUENCING are trademarks of 454 Life Sciences Corporation,

Branford, CT, USA.

© 2008 Roche Diagnostics GmbH. All rights reserved.

Roche Diagnostics GmbH

Roche Applied Science

68298 Mannheim, Germany

”Cells work quickly and precisely –

just like our Leica AM TIRF MC system.”

Our work is concentrated on depicting polarized protein transport in epithelial cells.

The incorporation of newly synthetisized proteins into the plasma membrane can be tracked in

the Leica AM TIRF MC microscope.

Prof. Dr. Ralf Jacob, University Marburg, Department of Clinical Cytobiology and Cytopathology,

Marburg, Germany

www.leica-microsystems.com

Better and Faster Systemsfor Protein Analysis

©2008 Promega Corporation, 16134-AD-GN

Now get enhanced digestion of even the most

difficult proteins and more complete peptide

recovery from gels. The combination of Promega

Trypsin Gold and ProteasMAX™ Surfactant gives you

maximum protein sequence coverage and increased

confidence of correct protein identification.

Express proteins in about one hour, not days,

and use them immediately with Promega

TNT® Systems. Unlike traditional E. coli-based

methods, the eukaryotic-based, cell-free TNT

System greatly enhances consistent expression

of functional proteins.

Learn more about utilizingcell-free expression tocharacterize proteininteractions in ourProtein Interaction Guide.

To request additional information and qualifyfor a free sample of the TNT® T7 Quick System,visit: www.promega.com/proteinanalysis

LeacellchaintPr

To request additiona

Panel A: Current standard: in-gel digestion of 47kDa protein band

from mouse cytoplasmic protein extract using Trypsin Gold.

Panel B: More complete in-gel digestion of 47kDa protein band

from mouse cytoplasmic protein extract using Trypsin Gold

with ProteasMAX™ Surfactant.

Access more information at www.7900HT.com

For Research Use Only. Not for use in diagnostic procedures.

Practice of the patented 5’ Nuclease Process requires a license from Applied Biosystems. The purchase of the TaqMan® Array includes an immunity from suit under patents specified in the product insert to use only the amount purchasedfor the purchaser’s own internal research when used with the separate purchase of an Authorized 5’ Nuclease Core Kit. No other patent rights are conveyed expressly, by implication, or by estoppel. For further information on purchasinglicenses contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. The TaqMan® Array is covered by U.S. Patents Nos. 6,514,750, 6,942,837, 7,211,443, and 7,235,406. MicrofluidicCard developed in collaboration with 3M Company. The Applied Biosystems 7900HT Fast Real-Time PCR System is a real-time thermal cycler covered by one or more of US Patents Nos. 6,814,934, 5,038,852, 5,333,675, 5,656,493,5,475,610, 5,602,756, 6,703,236, 6,818,437, 7,008,789, 6,563,581, 6,965,105 and 6,719,949 and corresponding claims in their non-US counterparts, owned by Applera Corporation. No right is conveyed expressly, by implication or byestoppel under any other patent claim, such as claims to apparatus, reagents, kits, or methods such as 5’ nuclease methods. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, AppliedBiosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

©2008 Applied Biosystems. All rights reserved. Applera, Applied Biosystems and AB (Design) are registered trademarks of Applera Corporation or its subsidiaries in the US and/or certain other countries. TaqMan is a registered trademarkof Roche Molecular Systems, Inc.

TaqMan® Arrays and the 7900HT Fast Real-Time PCR System. The Superior, Single-Solution for High-Throughput

Gene Expression Analysis.

Achieve optimal performance and flexibility in high-throughput gene expression analysis when you combine

TaqMan® Arrays with the 7900HT Fast Real-Time PCR System. Choose from pre-designed TaqMan® Gene

Signature Arrays, configurable TaqMan® Gene Sets, or TaqMan® Custom Arrays. Each format offers the accuracy,

reproducibility, and specificity you expect from an industry leader. Experience high-throughput and performance

synergy in a winning combination.

TaqMan® Arrays:

• Convenient for disease target classes and pathways

• Proven quality, speed, and ease-of-use

• Pre-loaded with TaqMan® Gene Expression Assays

• Increased sensitivity and precision

• Ideal for small and archival tissue samples

• Low sample consumption

• 384-well loading without liquid-handling robotics

7900HT Fast Real-Time PCR System

• Proven gold standard, industry-leading performance

• Interchangeable block formats offer easy application

adaptability and flexibility

• Seamless integration with TaqMan Arrays

• Automation Accessory provides hands-free plate-loading

and unloading for 24-hour operation

• Powerful software tools enable high quality data analysis

Unlock the Power of Gene Expression Analysis

with the Right Combination

Fashion Breakthroughof the Year

Our Science Gene SequenceT-shirt—get yours today!

By popular demand! Created to celebrate our Breakthrough

of the Year for 2007, this T-shirt is designed from an

annotated gene sequence map of human chromosome 1.

Since the shirt appeared on the cover of Science, we’ve been

flooded with requests. Now it’s yours for just $22.50 plus tax

(where applicable), and shipping & handling. Photos of the actual

shirt are available at the website below.

To order: www.aaas.org/go/geneshirt

PERSONALIZED MEDICINEPromises and Challenges

Discover how advances in tailoring treatmentcould change how you think about your health

9 am–noon

Anticipating PersonalizedMedicine: A Roundtable Discussion

Register at: aaas.org/spp/sfrl/PMRoundtable or fdli.org/conf/435/

• Explore novel issues arising from tailored drugs and companion diagnostics in realms likeIVD approval, labeling, laboratory testing, clinical treatment, and reimbursement

Registration Fee Required

• Stakeholders in clinical practice, research, patient groups, industry, insurance,and others will discuss personalizedmedicine’s societal implications

• Moderated by Susan Dentzer, health policy journalist and on-air correspondentfor the PBSNewsHour, Editor-in-Chief,Health Affairs

No Fee, But Registration Is Requested

1 pm–5 pm

Keynote Speaker

Mark B.McClellan,M.D., Ph.D., Senior Fellow andDirector,Engelberg Center for Health Reform, the Brookings Institution,

and former FDACommissioner

Implementing PersonalizedMedicine: A Legal, Regulatory, and Policy Seminar

Mark

Bradshaw,pixelweave.com

June 20, 2008 / AAASHeadquarters /Washington, D.C.

A Conference Sponsored bythe American Association for the Advancement of Science (AAAS)

and the Food and Drug Law Institute (FDLI)

Congratulationsto the AAAS Student Poster Winners!

AAAS recognizes the winners of the 2008 Student Poster Competition

on 16 February at the AAAS Annual Meeting in Boston. Their work in a

variety of �elds displayed originality and understanding that set them

apart from their colleagues. First-place winners will receive cash prizes

thanks to the generous support of Subaru of America, Inc.

Full abstracts can be viewed at

www.aaas.org/meetings

BRAIN AND BEHAVIOR

Winner: Amanda Carey and Christopher Shay, Northeastern

University

Potentiation of Drug Reward in a Mouse Model Expressing HIV-Tat

Protein

Honorable Mention: Jesse Weber, Harvard University

Evolution of Burrowing Behavior in Peromyscus

ENVIRONMENT AND ECOLOGY

Winner: Idalia Pérez, University of California, BerkeleyCalculations of the Chemical Composition of the Sacramento Urban

Plume

Honorable Mention: Amanda Ruiz, University of California, Irvine

Bottleneck and Massive Passage Dynamic Promote Drastic

Variation on RNA Virus Fitness

MATH, TECHNOLOGY, AND ENGINEERING

Winner:Marcus Allegood, Clemson University

Determining the Optical Properties of Biological Tissue Samples

MEDICINE AND PUBLIC HEALTH

Winner: Kameelah Abdullah, University of California, Irvine

Natural Genetic Polymorphism in the NS3 Protein of the Hepatitis C

Virus

Honorable Mention: Bethany Hedt, Harvard University

Matrix Pooling Testing Algorithm To Detect Acute HIV Infection and

Estimate Incidence

MOLECULAR AND CELLULAR

Winner: Ernesto Lujan, University of California, Irvine

FRT 42D and ovoD— Using a New Genetic Tool for a Large Scale

Maternal Effect Screens

Honorable Mention: Shih-Wen Lin and Scott Hensley, University of

Pennsylvania

Recombinant AAVs Induce Functionally Impaired Transgene

Product-Speci�c CD8+ T Cells

PHYSICAL SCIENCES

Winner: Kevin Ro, University of California, Los Angeles

Global Analysis of Ubiquitinated Proteins — A Shotgun Proteomics

Approach

Honorable Mention: Andre Paredes and Wei Xu, University of

California, Irvine

Polymer Based Micro Flow Regulator

SCIENCE AND SOCIETY

Winner:Meagan Flenniken, Cornell University

Reindeer Nutrition in the Mongolian Taiga: Advancing Community

Development

Honorable Mention: Brian Pompeii, Arizona State University

Private Water Companies in a Desert City

SOCIAL SCIENCES

Winner: Hugo Reyes-Centeno, Stanford University

The Etruscans: A Geometric Morphometrics Cranial Study

Honorable Mention: Alexandra Glorioso and Shade Shutters,

Arizona State University

International Agreements and Their Effects on Globalization

Bookmark www.aaas.org/meetings for details on

the 2009 AAAS Annual Meeting, 12-16 February. The

Poster Submission site opens on 8 July 2008.

The Student Poster Competition recognizes the individual efforts

of undergraduate and graduate students working toward a degree.

Posters are judged at the meeting. Winners in each category receive

a cash award and framed certi�cate. Postdoctoral scholars who

hold a doctoral degree are not eligible to enter. The AAAS General

Poster Session is open to postdocs and professionals.

What makes a fi rst-class news story?

Jennifer CouzinArticles selected for inclusionin The Best American ScienceWriting 2007 and 2005

2003 Evert Clark/Seth PayneAward for Young ScienceJournalists

Richard Kerr2006Geological Society of America: Public ServiceAward

Colin NormanNews Editor,Sciencemagazine

A first-class editorial team. Award-winning

journalists write for Science—with 12 top awards

in the last four years. That’s why we have the most

compelling stories, and the biggest readership of

any general scientific publication. To see the

complete list of awards go to:

sciencemag.org/newsawards

Constance Holden2004National MentalHealth Association:Media Award

www.sciencemag.org/products SCIENCE VOL 320 9 MAY 2008 815

AAAS/Science Business Office

New Products Focus: Cell/Tissue Culture

LIFE SCIENCE TECHNOLOGIES

High Throughput Transfection SystemCellaxessHT is an automated, high throughput transfection system that enables reagent-freedelivery of small interfering RNA and complementary DNA to a wide range of biologicallyrelevant cell typeswith excellent transfection efficiencies and viabilities. The system featuresa patented capillary electrode array technology for transfection of cells cultured in 384-wellplates and offers a capacity of up to 50,000 wells per day.

CellectriconFor information +46 (0) 31 760 35 15

www.cellectricon.com

RNA StabilizerRNAstable is for preserving RNA at room temperature, whicheliminates the need for cold storage and refrigerated shipping.RNA samples are difficult to work with given their labile natureand tendency to degrade even in cold storage. Exposure to slightlyelevated temperatures for even a short time can compromiseRNA integrity. RNAstable makes use of technology that forms athermostable barrier, securely “shrink wrapping” RNA samples toprotect them from degradation. To store RNA, researchers simplypipette samples directly into a tube or well of RNAstable and letthem dry. Sample recovery requires only adding water, with nofurther purification required. RNAstable preserves RNA at roomtemperature for up to five months and is better at preventingdegradation than freezer storage. The preserved samples can beused in applications such as bioanalyzer and microarray analysis,end-point polymerase chain reaction, gel analysis, reversetranscription, and more without inhibition or interference.BiomatricaFor information 858-550-0308www.biomatrica.com

Profiling ArrayThe T7-oligonucleotide ligation assay-based microRNA (miRNA)profiling array allows the user to access miRNA expression costeffectively. Expression of multiple miRNAs can be determinedsimultaneously in a single, simple assay that does not requirepreisolation of small RNA. The analysis has the power todifferentiate miRNA isoforms with a single nucleotide differenceand to differentiate all miRNA isoforms. Captured miRNAs aresubjected to T7 amplification without introduction of bias inpolymerase chain reaction. Total RNA can be used in the assaydirectly, without preisolation of miRNA.SignosisFor information 877-266-8388www.signosisinc.com

Capillary-Mimicking PlatformThe VenaFlux Platform is a semiautomated, high throughput,microfluidic cell-based assay system. It measures cell adhesionto antibody-coated or endothelial-cell cultured microchannels,producing curves under shear stress conditions that mimicphysiological flows. The VenaFlux is simple to use, reduces costlylayers of animal model studies, and delivers specific, accurate,and reproducible results. Scientists can use the VenaFlux torapidly obtain quantitative analyses and results for potentialdrug candidates in cardiovascular, respiratory, immunological,autoimmune, and oncological diseases. These analyses can reducedrug development costs by accelerating false-lead elimination.

Newly offered instrumentation, apparatus, and laboratory materials

of interest to researchers in all disciplines in academic, industrial, and

governmental organizations are featured in this space. Emphasis is

given to purpose, chief characteristics, and availabilty of products and

materials. Endorsement by Science or AAAS of any products or materi-

als mentioned is not implied. Additional information may be obtained

from the manufacturer or supplier.

Electronically submit yournewproductdescriptionorproduct literature

information! Go to www.sciencemag.org/products/newproducts.dtl

for more information.

The VenaFlux replicates cell behavior in human capillaries,which is an expanding area of research. The technology providesresearchers with a single platform for executing dynamic studiesto analyze the effects of drugs on cell adhesion, proliferation, andtransmigration under well-defined shear stress protocols thatreplicate physiological conditions.CellixFor information 917-623-4456www.cellixltd.com

RNAi DuplexesPlateSelect RNA interference (RNAi) duplexes offer the ability tocustomize 96-well plates with Invitrogen’s chemically modifiedStealth or standard BLOCK-iT RNAi duplexes. Users develop theircustomized plates through an intuitive web interface, and they canorder from one to 96 different duplexes in a single plate. The RNAiduplexes are synthesized on demand to take advantage of themostup-to-date bioinformatics and are provided at 1 nmol/well in thewell orientation that the customer chooses. The duplexes arrive inready-to-transfect format and are synthesized based on algorithmsdesigned to reduce the chances of off-target effects.InvitrogenFor information 800-955-6288www.invitrogen.com/plateselect

RNA Purification KitThe MasterPure RNA Purification Kit offers increased yields of totalRNA, compared with methods such as spin-column kits. Startingwith tissue, eukaryotic cells, or bacterial culture, the gentle saltprecipitation preserves microRNAs and other small RNAs thatwould be lost with a spin-column kit. Up to 25 µg of RNA can beobtained from 5mg of mouse liver.Epicentre BiotechnologiesFor information 800-284-8474www.EpiBio.com

New Products Focus: RNAi

Get the latest news and research from Science as soon as it is published. Sign

up for our e-alert services and you can know when the latest issue of Science

or Science Express has been posted, peruse the latest table of contents for

Science or Science Signaling, and read summaries of the journal’s research,

news content, or Editors’ Choice column, all from your e-mail inbox. To start

receiving e-mail updates, go to:

Science Alertsin Your Inbox

ScienceMagazine TOCWeekly table of contents

Science Posting NotificationAlert whenweekly issue is posted

ThisWeek inScienceSummaries of research content

Editors’ ChoiceHighlights of therecent literature

ScienceNewsThisWeekBrief summaries of thejournal’s news content

Science ExpressNotificationArticles published inadvance of printScienceNOWWeekly Alert

Weekly headline summary

ScienceNOWDaily AlertDaily headline summary

Science Signaling TOCWeekly table of contents

sciencemag.org/ema

Get daily andweekly E-alerts on the latest breaking news and research!

FREE

withregistration

R&D Systems Tools for Cell Biology Research™

USA & Canada R&D Systems, Inc. Tel: (800) 343-7475 [email protected]

Europe R&D Systems Europe, Ltd. Tel: +44 (0)1235 529449 [email protected]

Selection expanding weekly—visitwww.RnDSystems.com/go/request to sign up for weekly new product updates.

R&D Systems Cell-Based ELISAsMeasure Intracellular Proteins in Intact Cells

Measure total and phospho-proteinssimultaneously in the same well

No specialized equipment needed

No lysate preparation required

Results with as few as 10,000 cells/well

Fixed cell

Substrate F2Substrate F1

Emission

600 nm

Anti-phospho-protein

PHOSPHORYLATED PROTEIN TOTAL PROTEIN

Anti-total-protein

Emission

450 nmHRP AP

Ph

osp

ho

ryla

tio

no

fE

RK

1/E

RK

2(N

orm

aliz

ed

RF

Us)

Concentration of Inhibitor U0126 (µm)

Unstimulated

2000 0.8 4 20 100

1000

600

1400

EGF

EGF-induced ERK1/ERK2 phosphorylation wasmeasured in A431 cells using

thePhospho-ERK1/ERK2(T202/Y204)Cell-BasedELISA(Catalog#KCB1018).

EGF-induced ERK1/ERK2 phosphorylationMOLECULE SPECIES CATALOG #

Akt Human, Mouse KCB887

EGF R/ErbB1 Human KCB1095

ERK1/ ERK2 Human, Mouse, Rat KCB1018

HIF-1α Human, Mouse KCB1935

HSP70 Human, Mouse KCB1663

MEK1/MEK2 Human, Mouse, Rat KCB2506

p38 Human, Mouse KCB869

p70 S6 Kinase Human, Mouse KCB8963

PDGF Rβ Human KCB2316

PDGF Rβ Human KCB1767

STAT6 Human, Mouse KCB3717

Now Available from R&D Systems

For more information visit our website atwww.RnDSystems.com/go/CellBasedELISA