Genomics for Triticeae improvement FP7 European Project.

Genomics for Triticeae improvement

FP7 European Project

OverviewOverview

Achieve significant progress in Triticeae genomics and support efficient

breeding of improved varieties for European agriculture

European Seventh Framework Programme project (financed by the EC)

Project duration: 4 years – From June 2008 to Mai 2012

Budget: 7,5 million Euro (5,3 million Euro supported by the EC)

Project Coordinator: Catherine Feuillet (INRA)

Activities: 5 research WPs, 1 WP for project dissemination and 1 WP for

project management

OverviewOverview 17 partners (including 2 breeders) from 9 countries

Public

Private

Activities and WPsActivities and WPs

WP1: Physical mappingWP1: Physical mapping

Of wheat group 1 and 3 chromosomes

Construction of BAC libraries

Fingerprinting of BAC libraries

Assembly of BAC contigs

BAC libraries pooling from the MTP and rearraying for

BES (by INRA-CNRGV)

BAC end sequencing of the MTPs for marker

development and genome analysis

WP1: Physical mappingWP1: Physical mapping

Assembly of BAC contigs

• Chromosome 3B: assembly by INRA (France)

• Chromosome 3D: assembly of 3DS by IEB (Czech Republic),

and assembly of 3DL by JIC (UK)

• Chromosome 1B: assembly of 1BS by HU (Israel), and

assembly of 1BL by INRA (France)

• Chromosome 1A: assembly of 1AS by UZH (Switzerland), and

assembly of 1AL by SU (Turkey)

WP2: Physical maps anchoringWP2: Physical maps anchoring

Of wheat and barley group 1 and 3 chromosomes

Establishment of a marker repository: including only markers for

which sequences are available

Generating resources for high resolution genetic mapping: For

wheat, creation of a population of CS x Re RILs of about 2000

plants. For barley, creation of Morex x Barke RILs.

Anchoring BAC contigs to chromosomes by high throughput

screening methods: forward and reverse anchoring

Comparative genetic and physical mapping with other grass

genomes

WP3: WP3: Gene isolation and new alleles discoveryGene isolation and new alleles discovery

Isolate five agronomically important genes and QTLs (4 in wheat and 1

in barley)

Trait Species Chr. Partner

Stripe rust resistance YrH52 Wild emmer wheat

1BS HU

Wheat quality (pentosan viscosity) PV-QTL

Bread wheat 1BL INRA

Glume blotch resistance

QSng.sfr–3BS

Bread wheat 3BS UZH

Grain yield QYld.idw-3BS Durum wheat 3BS UNIBO

Plant architecture (tillering) cul4

Barley 3H UMIL

WP3: WP3: Gene isolation and new alleles discoveryGene isolation and new alleles discovery

High-resolution mapping of the genes underlying the

target traits

Identification of candidate genes

Functional validation of candidate genes

New alleles discovery

WP4: Molecular markers for breedingWP4: Molecular markers for breeding

WP leading by the breeding companies

Development of trait specific markers: using markers from

WP1 (BAC end sequencing) and WP2

Establishment of an European diversity panel for

association mapping: panel contains 730 varieties (171 from

UK, 178 from Germany and 381 from France)

The data generated will be useful for all the chromosomes.

WP5: WP5: Bioinformatics tools and databasesBioinformatics tools and databases

For wheat and barley

Development of a web interface integrating genetics and physical

maps

Development of a new analytical framework for contig assembly:

LTC (more robust than FPC)

Development of comparative genome informatics tools: with

Brachypodium, rice, sorghum, etc…

Development of a new version of TriAnnot pipeline for automated

sequence annotation

WP6: WP6: Dissemination, technology transfer and trainingDissemination, technology transfer and training

Organisation of 9 training courses in the different

partners instituts

Development of the Project website

http://www.triticeaegenome.eu/

Dissemination at scientific and technological events:

talks/posters, production of a project leaflet and a poster

WP7: Project ManagementWP7: Project Management

Scientific and technical management

Administrative and financial management: organization of

the different project meetings, monitoring of the project costs

Communication and interactions between partners and

other projects: establishment of a Collaborative Platform,

interaction with the Triticeae Genomics Network (TGN)

Technical and financial reporting to the EC: detailed

reports that describe the work progress, costs incurred and other

justifications for the reimbursement of project costs

UC Davis

Kansas SU

CSIC

Viterbo U

Modena U

CRA-CER Foggia

Murdoch U CSIRO

UC Riverside

Iowa SU

Okayama U

Minnesota SU

CAAS Beijing

CIMMYT

MPI Cologne

ACPFG

A large network of international collaborators

INTA

UMB