Genetic Testing Jeopardy! Waterford Union High School.

Genetic Testing Jeopardy!

Waterford Union High School

Rules

• Each team sends one person per turn. They cannot get help from their team

• First to “buzz” in gets 15 seconds to answer. • If the first person to buzz cannot get the answer, the

second team can buzz in. Once they buzz in, they too have 15 seconds. They also cannot get help.

• You either gain or lose points on each turn.• If no team buzzes within 10 seconds, the question

ends and the answer is given.

$100 $100 $100 $100 $100 $100

$200 $200 $200 $200 $200 $200

$300 $300 $300 $300 $300 $300

$400 $400 $400 $400 $400 $400

$500 $500 $500 $500 $500 $500

SangerMethod

PCRElectroph.

DNA FP’s & RFA

SouthernBlotting

ELISA This & That

Sanger Method

• This is a “fake” nucleotide that is dyed and allows DNA to be read letter by letter by stopping the addition of bases at random points

• ddNTPs

Sanger Method

• This separates the DNA fragments from longest to shortest

• Electrophoresis Gel

Sanger Method

• These are the kind of cells we’d use to sequence DNA in most cases

• White blood cells

Sanger Method

• These are the portions of DNA that code for actual genes and have an Open Reading Frame

• Exons

Sanger Method

• This is what is used to replicate the DNA for the Sanger Method

• E. coli

PCR Electrophoresis

• These are the regions of DNA that have random repeats of short sequences of bases

• STRs

PCR Electrophoresis• Based on the information in the table below,

this suspect’s DNA was found at the scene of the crime

• Suspect 2

PCR Electrophoresis

• This is the enzyme taken from hot springs that is used in PCR to make copies of DNA in a heating/cooling environment like that of PCR

• Taq polymerase

PCR Electrophoresis

• DNA is denatured in PCR using this• Heat

PCR Electrophoresis

• This is the term for the process of separating DNA fragments after they’ve been cut and amplified

• Electrophoresis

DNA Fingerprints & Restriction Fragment Analysis

• This is when variations in a person’s DNA are examined to determine what aspects are unique to an individual

• DNA Fingerprinting

DNA Fingerprints & Restriction Fragment Analysis

• This where patterns created by cut DNA in a gel are examined to determine the source of DNA, paternity, or diagnose a disease.

• Restriction Fragment Analysis

DNA Fingerprints & Restriction Fragment Analysis

• This kind of DNA is used to create the DNA Fingerprint because it contains the most variability and STRs

• Introns

DNA Fingerprints & Restriction Fragment Analysis

• Human DNA is ___% the same from person to person

• 99.9

DNA Fingerprints & Restriction Fragment Analysis

• This is necessary to see the DNA after it is run through a gel

• Dying

Southern Blotting

• This is the genetic test we’d use to diagnose a genetic disease

• Southern Blotting

Southern Blotting

• To conduct Southern Blotting, we’d use this to transfer DNA from the gel.

• A membrane

Southern Blotting

• To conduct Southern Blotting, we’d use this to find a specific gene in question due to the fact that this has a complementary sequence to that DNA

• Probe

Southern Blotting

• A probe will usually do this if a gene is present• Glow (bioluminescence)

Southern Blotting

• Southern Blotting does not work for this kind of disease

• Infectious

ELISA

• This is the kind of test we’d use for infectious disease.

• ELISA

ELISA

• ELISA can detect infectious disease by looking for this substance

• Proteins (or antibodies)

ELISA

• These are produced to identify substances in your body

• Antibodies

ELISA

• These are recognized by antibodies and are a sign of a disease in the body

• Antigens

ELISA

• During ELISA, if a disease is present, the blood sample will ______

• Show a color

This & That

• This is the kind of DNA that codes for a protein• Exon

This & That

• This is the kind of DNA that does not code for a protein

• Intron

This & That

• This is how we know if a stretch of DNA is an exon or an intron

• Open Reading Frame

This & That

• This is the term for an antibody generator• Antigen

This & That

• This is the enzyme that cuts DNA• Restriction enzyme