Genetic Indicators Genetic Indicators Research Research Molecular Ecology Molecular Ecology Research Branch Research Branch NERL NERL Overview- • Genetic diversity within species • Gene induction (expression) research • Some considerations for transplanting genetically resistant organisms
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Genetic Indicators Research Molecular Ecology Research Branch NERL Overview- Genetic diversity within species Gene induction (expression) research Some.
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Genetic Indicators Genetic Indicators Research Research Molecular Ecology Molecular Ecology
Research BranchResearch Branch
NERLNERL
Overview- • Genetic diversity within species• Gene induction (expression)
research• Some considerations for
transplanting genetically resistant organisms
Research GoalsResearch Goals
GPRA-• Goal 8.1.1 - By 2008, develop indicators
and monitoring designs for a national stream and coastal survey.
• FY00 APM - A draft report on the feasibility of genetic and molecular biomarkers as an indirect measure of pesticide, toxics, and metals exposure to fish in the Eastern Cornbelt Plains ecoregion.
Some considerations Some considerations regarding transplantation regarding transplantation of adapted organisms and of adapted organisms and
communitiescommunities
Transplantation IssuesTransplantation Issues
1. Is the population really adapted to mining impact area?
• Adaptation or acclimation?• Bioavailability differences
between sites?• Adapted to same or different
stressor profiles?
Transplantation IssuesTransplantation Issues
2. Will the mitigation create new problems?
• Will the transplants interbreed with nearby locally adapted populations?
• Will the population need constant supplementation?
• Will an artificial selection program need to be created?
Transplantation IssuesTransplantation Issues
3. Research need: an ability to predict population responses to stressors: acclimation, adaptation, or extinctionDepends on:• Magnitude and novelty of stressor• Number of genes involved in response• Genetic diversity of response genes• Rate of environmental change
Possible contributions by EPA genetics researchers
• Assessment of bioavailability differences between sites
– Metallothionein gene expression• Assessment of population structure/
gene flow within region– Most appropriate transplant populations– Potential for introgression with native