Genetic Engineering

Review Game

GENETIC ENGINEERING

Best Arnold character

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1. Terminator2. Predator3. Mr. Freeze4. Kindergarten Cop

1.

2.

3.

4.

DNA that is from two or more organisms is called…

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1. Combinant DNA2. Remade DNA3. Recombinant DNA4. Multiple DNA

Fastest Responders (in seconds)4.91 KAYLA COCKRELL5.69 ETHAN WHITE6.09 NORA WAARANIEMI7 EMILY TURNQUIST7.6 MICHAEL GIBBS

Today’s Topics

Genetic Engineering

Stem CellsGel Electrophoresis

GMOs and Cloning

Final Results

Genetic Engineering

Which of these is not an example of a vector used in genetic engineering?

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1. Plasmid2. Yeast3. Virus4. Bacteria

What is used to cut DNA?

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1. Restriction Enzymes2. DNA Ligase3. Scissors4. Recombinant Enzymes

If I wanted to produce human insulin using a transformed bacteria, what would I need to do first?

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1. Recombine the DNA with a vector

2. Cut out the gene of interest

3. Isolate the gene of interest

4. Insert the gene into the bacteria

I have now made my Recombinant DNA. What must take place before I can clone the gene?

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1. Transform the bacteria2. Select and screen3. Gene cloning4. Use DNA Ligase

DNA fragments cut with EcoRI can be combined with DNA cut with

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1. BamHI2. HindIII3. EcoRI4. All of the above5. 1 & 26. 2 & 3

Restriction fragments cut with a restriction enzyme have a single- stranded 4 base tail of AATT. What is this called?

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1. Blunt end2. Sticky end3. Restriction tail4. Ligase

How does the gene get cloned once it has been taken in by the bacteria?

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1. Using PCR2. Do more

transformations3. Bacterial reproduction4. Gel electrophoresis

Once I have selected and screened the bacteria, I can now …

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1. Extract the gene2. Extract the protein3. Give the bacteria to an

ill person4. All of these5. 1 & 26. 1 & 3

Fastest Responders (in seconds)11.89 MICHAEL GIBBS15.57 KATHERINE

SPURLOCK18.42 THOMAS

NETTLETON32.7 SAMUEL KAUTZKY

Team Scores63.33 Terminator53.75 Kindergarten Cop51.1 Mr. Freeze50 Predator

GMOs and CloningFASTEST FINGER

Which is easier to clone?

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1. Animals2. Plants3. Humans

Which of these is not a reason for making crops GMOs?

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1. Better Taste2. Drought Resistance3. Pest Resistant4. All of the above5. Only 2 and 3

If I wanted to clone a sheep 1, using an egg from sheep 2, and having sheep 3 deliver the baby, where do I get the DNA from?

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1. Sheep 12. Sheep 23. Sheep 3

The method of cloning described in the last slide is called?

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1. Nuclear replacement2. Somatic cell nuclear

transplantation3. Artificial twinning4. Egg cell fertilization

Which type of cell can I get the DNA from?

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1. Heart cell2. Skin cell3. Liver cell4. Muscle cell5. None of these6. All of these

Fastest Responders (in seconds)10.77 NORA WAARANIEMI11.11 BRANDON LINGEN12.1 KATHERINE

SPURLOCK12.79 KYLIE JOHNSON19.38 LIESE PETERSON

Team Scores88.2 Terminator78.65 Mr. Freeze77.52 Kindergarten Cop75.77 Predator

Gel ElectrophoresisFASTEST FINGER

Gel Electrophoresis does what?

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1. DNA Fingerprinting2. Paternity testing3. Separates molecules

by size4. Uses electricity

Since everyone on the planet has different DNA, identical twins excluded,

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1. No one has the same DNA fingerprint

2. Most people have a different DNA fingerprint

3. You are less likely to have the same DNA fingerprint as another

In RFLP analysis, with what do we mix our DNA samples before running them through a gel

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1. DNA ligase2. plasmids3. Restriction enzymes4. DNA polymerase

Who is the baby’s father?

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1. Bob 2. Larry3. Neither

Which direction would DNA move while being separated in gel electrophoresis?

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1. Towards the positive2. Towards the negative3. Both directions

depending on the person

Which DNA Fragment is shorter?

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1. A2. B

Who matches the blood stain?

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1. Bob2. Sue3. John4. Lisa

PCRs main function is to

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1. Exponentially Amplify DNA

2. Make a few copies of DNA

3. Catch criminals4. Extract DNA

Fastest Responders (in seconds)3.13 MICHAEL GIBBS5.69 ERIN LOUWAGIE6.01 KEVIN STOWE8.33 NORA WAARANIEMI8.58 CALEB TROUTH

Team Scores117.94 Predator117.78 Terminator110.98 Mr. Freeze102.19 Kindergarten Cop

Stem Cells

Which of these can differentiate into the widest variety of cells?

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1. Totipotent2. Multipotent3. Pluripotent

Embryonic stem cells are

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1. Totipotent2. Pluripotent3. Multipotent

Where do most adult stem cells used in medicine come from?

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1. Kidneys2. Skin3. Blood4. Bone marrow

Adult stem cells are

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1. Totipotent2. Pluripotent3. Multipotent

Which type of stem cell is created from a differentiated somatic cell?

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1. Adult stem cells2. Embryonic stem cells3. Induced pluripotent

stem cells

Fastest Responders (in seconds)4.87 MICHAEL GIBBS

Team Scores142.76 Mr. Freeze137.94 Predator137.78 Terminator132.19 Kindergarten Cop

Team MVPPoints Team Participant179.91 Mr. Freeze MICHAEL GIBBS 147.07 Predator ERIN LOUWAGIE 151.84 Terminator TIFFANY LO 163.27 Kindergarten Cop EMILY TURNQUIST

Participant Scores179.91 MICHAEL GIBBS 129.4 TRISHA MORRISON165.66 BRANDON LINGEN 126.43 TAYLOR HAEFELE163.27 EMILY TURNQUIST 120.2 RISHA SINGH151.84 TIFFANY LO 118.49 KAYLA COCKRELL150.67 CALEB TROUTH 118.43 KYLIE JOHNSON147.07 ERIN LOUWAGIE 115.6 PETER KILIAN141.37 KATHERINE

SPURLOCK112.91 PETER FOMIN

135.43 LIESE PETERSON 112.38 KEVIN STOWE132.76 NORA WAARANIEMI 103.77 THOMAS

NETTLETON132.11 ETHAN WHITE 101.31 LYDIA BOIKE