Genetic correlations and associative networks for CNS transcript abundance and neurobehavioral phenotypes in a recombinant inbred mapping panel Elissa J. Chesler, Jintao Wang, Lu Lu, Jeremy L. Peirce, Yanhua Qu, Kenneth F. Manly, Robert W. Williams University of Tennessee-Memphis Health Science Center
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Genetic Correlations: Relation of trait values in genetically similar individuals
Genetic correlations and associative networks for CNS transcript abundance and neurobehavioral phenotypes in a recombinant inbred mapping panel. Elissa J. Chesler, Jintao Wang, Lu Lu, Jeremy L. Peirce, Yanhua Qu, Kenneth F. Manly, Robert W. Williams University of Tennessee-Memphis - PowerPoint PPT Presentation
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Genetic correlations and associative networks for CNS transcript
abundance and neurobehavioral phenotypes in a recombinant inbred
mapping panel
Elissa J. Chesler, Jintao Wang, Lu Lu, Jeremy L. Peirce, Yanhua Qu, Kenneth F. Manly, Robert W.
Williams
University of Tennessee-MemphisHealth Science Center
Genetic Correlations: Relation of trait values in genetically similar
individuals
• Which traits share a common genetic mediation?
(Genetic Correlations)
• Which genes have expression levels associated with the trait?
(Genetic Correlations)
• Where are the polymorphisms that influence the traits?
(Trait to Marker Correlations - Quantitative Trait Loci)
BEHAVIOR
45
50
55
60
65
70
75
80
mRNA LEVEL
8.4
8.6
8.8
9
9.2
9.4
9.6
9.8
BXD Recombinant Inbred Strains • Several hundred phenotypes have been studied ( > 500 ).
• Brain expression of several thousand genes have been measured using Affymetrix microarrays.
• 750+ genetic marker strain distributions are known.• All data is available at www.webqtl.org• No additional genotyping is necessary to perform genetic analyses.
C57BL/6J (B)C57BL/6J (B) DBA/2J (D)DBA/2J (D)
F1F1
20 generations
brother-sister
matings
20 generations
brother-sister
matings
BXD1BXD1 BXD2BXD2 BXD80BXD80+ … ++ … +
F2F2
BXD RIStrain setBXD RI
Strain set
fullyinbredfully
inbred
isogenicisogenic
hetero-geneoushetero-
geneous
Recombined chromosomes are needed for
mapping
Recombined chromosomes are needed for
mapping
femalefemale malemale
chromosome pairchromosome pair
InbredIsogenicsiblings
InbredIsogenicsiblings
BXDBXD
Search the BXD Published Phenotypes and Gene Expression Databases at
www.webqtl.org
Affymetrix Gene Expression Data in BXD RI mice
High density Marker Map for Re-analysis of BXD Phenotypes
The marker map used for BXD RI expression QTL mapping. The current map has ~750 markers
Pair-wise recombination fractions (upper triangle) and LOD scores for linkage of markers (lower triangle). Warmer colors indicate greater linkage. Plots were created using R/QTL.
C57BL/6J
DBA/2JMarker-Trait Associations
Correlation Analysis Options and Diagnostic Plots
Evaluating Linearity and Univariate Normality
Drd2 expression is determined by genetic variation near the