Genetic Basis of Development & Biotechnologies 1. Steps of embryonic development: cell division, morphogenesis, differentiation • Totipotency and pluripotency 2. Plant cloning 3. Animal cloning • Reproductive versus therapeutic cloning • Nuclear transplantation • Stem cells: adult and embryonic • Somatic Cell Reprogramming (2007)/ Induced Pluripotent Stem Cells (iPSC) 4. Molecular mechanisms of embryonic morphogenesis and differentiation 5. Animal body plan: homeotic genes 6. Cell death: necrosis and apoptosis
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Genetic Basis of Development & Biotechnologies 1. Steps of embryonic development: cell division,
morphogenesis, differentiation • Totipotency and pluripotency
4. Molecular mechanisms of embryonic morphogenesis and differentiation
5. Animal body plan: homeotic genes 6. Cell death: necrosis and apoptosis
Genetic Basis of Development
From a diploid zygote to a multi-cellular organism
Sperm cell
Nuclei containing DNA
Egg cell
Fertilized egg with DNA from both parents
Embyro’s cells with copies of inherited DNA
Offspring with traits inherited from both parents
Three processes of embryonic development: • Cell division- increase n cell number • Morphogenesis- “creation of form” • Cell differentiation- specialization in
structure and function
Embryonic Development • a single-celled zygote many different types
of cells • higher-level structures organs arranged in a
particular way in three dimensions • cells-- tissues--- organs--- organ systems–
whole organism
Cell & tissue movement
Growth in size
Animals Necessary for embryonic transformation
Limited to embryonic and juvenile stages
Plants Does not take place
Continues throughout the life of the plant
Morphogenesis
Totipotent cells (any) Pluripotent cells (many
Human morphogenesis disorder Cleft palate- upper wall of the mouth cavity
fails to close completely
Differentiation produces a variety of cell types, each expressing a different combination of genes
Muscle cell Pancreas cells
Blood cells Nerve cell
Plant cloning Used extensively in agriculture
Plant cell remain totipotent and can dedifferentiate.
Cloning of Organisms
Animal Cloning
• Reproductive Organism
• Therapeutic Tissues & Organs
Animal cloning
Nuclear transplantation
-only 2% develop normally from nuclei of differentiated cells
Different types of cell in an organism have the same DNA but they transcribe different genes
Nuclei do change as cells differentiate: DNA sequences do not change Chromatin structure and methylation patterns do
The possibility of cloning humans raises unprecedented ethical issues.
Stem Cell Research Stem cells
– unspecialized cells, continually reproduce can differentiate into specialized cell types.
– can differentiate into multiple cell types are multipotent or pluripotent.
Two types of stem cells 1. Adult stem cells & Cord Blood stem cells 2. Embryonic stem cells
Under the right conditions, cultured stem cells derived from either source can differentiate into specialized cells.
Omnipotent
Adult stem cells • Pluripotent: bone marrow stem cells-
different kinds of blood cells
Embryonic stem cells • Totipotent- immortal
Somatic Cell reprogramming (2007) Induced Pluripotent Stem Cells (iPSC) Oct 20 2009, 11:21 AM EST Induced Pluripotent Stem Cell Technology Used to Generate Hepatocytes from Skin Cells GEN News Highlights http://learn.genetics.utah.edu/content/tech/stemcells/ips/
Molecular Genetic basis of Cancer • Mutations affecting control sequence of genes or coding sequences of genes • Movement of DNA within the genome • Gene amplification
Genetic Testing & Personalized Medicine
(Learning Objectives)
1. Recognize the presence of common mutation within members of the human population (polymorphisms)
2. Recognize that information about such polymorphisms can be used for several purposes, such as:
• Mutational analysis of disease causing genes • Genome –wide scanning for disease predisposition genes • Personalized Medicine
Variations in the DNA sequences of humans affect :
- Disease development - Response to: toxins, drugs, vaccines,
and chemotherapy. http://www.youtube.com/watch?v=dUL5f8nB