Gel electrophoresis L Mathias. Definition Separation of DNA fragments according to size, based on movement through a gel medium when an electric field.

Gel electrophoresis

L Mathias

Definition

• Separation of DNA fragments according to size, based on movement through a gel medium when an electric field is applied.

DNA negatively charged

DNA cut up using restriction endonucleases

Make up the gel which the DNA will be put into

Dye added to the DNA

Buffer solution added to the tank

DNA samples loaded into wells

Electrical current applied to the chamber

DNA is stained using ethidium bromide

This powerpoint was kindly donated to www.worldofteaching.com

http://www.worldofteaching.com is home to over a thousand powerpoints submitted by teachers. This is a completely free site and requires no registration. Please visit and I hope it will help in your teaching.